BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0023.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGT8.1 B1DGT8_9BACL Binding-protein-dependent transport system... 490 e-137 B3KKB5.1 B3KKB5_9BACI Binding-protein-dependent transport system... 335 4e-90 B1DFA0.1 B1DFA0_9BACL Binding-protein-dependent transport system... 280 1e-73 B1D8A6.1 B1D8A6_9BACL Binding-protein-dependent transport system... 278 3e-73 B3KCR9.1 B3KCR9_9BACI Binding-protein-dependent transport system... 277 1e-72 >B1DGT8.1 B1DGT8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 319 Score = 490 bits (1262), Expect = e-137, Method: Compositional matrix adjust. Identities = 247/293 (84%), Positives = 264/293 (90%) Query: 1 MKVYWPLYVMAIPGIIFLIVFKFIPLAGAVIAFKDYSVFRGFIDSPWVGFKHFETLLQHP 60 MKVYWPLY MAIPGIIFL+VFK+IPLAGAVIAFKDYSVF+GFIDSPWVGFKHF+TLL HP Sbjct: 27 MKVYWPLYAMAIPGIIFLVVFKYIPLAGAVIAFKDYSVFKGFIDSPWVGFKHFDTLLHHP 86 Query: 61 DFLRVFSNTLLLGLFKLTIVFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIVGG 120 DF RVF NTL+LGL KL +VFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIV G Sbjct: 87 DFARVFGNTLMLGLLKLVLVFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIVAG 146 Query: 121 ILFDFFSLSGMFNIILGWFGAEPVLAMQESAYFRPVYVLASIWKDAGWGTVVYMAAISAI 180 I+FDFFSLSG+FNI+LGWFG EPVLAMQES YFRPVYV++SIW+DAGWGTVVYMAAISAI Sbjct: 147 IVFDFFSLSGLFNIVLGWFGQEPVLAMQESDYFRPVYVISSIWRDAGWGTVVYMAAISAI 206 Query: 181 DPQLYESAMIDGASRFRQIRHIXXXXXXXXXXXXXXXXXGNFLDLGFDQVYNLLTPMTYN 240 DPQLYESAMIDGAS+FRQ+RHI GNFLDLGFDQV+NL+TPMTYN Sbjct: 207 DPQLYESAMIDGASKFRQMRHITFPLLLPTVLVLFLLEIGNFLDLGFDQVFNLITPMTYN 266 Query: 241 VGDILDTYVFRTGIQQGQYSFATAVGLFQSVIGFIMVYTFNKLSNKVSDGGLW 293 VGDILDTYVFRTGIQQ QYSFATAVGLFQSVIGFI+VY FNKLS KVSDGGLW Sbjct: 267 VGDILDTYVFRTGIQQAQYSFATAVGLFQSVIGFILVYMFNKLSKKVSDGGLW 319 >B3KKB5.1 B3KKB5_9BACI Binding-protein-dependent transport systems inner membrane component Length = 321 Score = 335 bits (858), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 155/293 (52%), Positives = 216/293 (73%), Gaps = 1/293 (0%) Query: 1 MKVYWPLYVMAIPGIIFLIVFKFIPLAGAVIAFKDYSVFRGFIDSPWVGFKHFETLLQHP 60 ++ +W LY+M IPGI++ IVFK++PLAG+VIAF+DY +F+G + SPWVG +F + + Sbjct: 30 IRKHWMLYLMIIPGILYYIVFKYVPLAGSVIAFQDYQIFKGILGSPWVGLDNFGFIFSYQ 89 Query: 61 DFLRVFSNTLLLGLFKLTIVFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIVGG 120 DF V NT ++ ++L FP P++LAL+ NEIR K+ +Q+ Y+PHFLSWV+VGG Sbjct: 90 DFYNVLRNTAVIAFYQLVFGFPAPIVLALLFNEIRIMLAKRVVQSMFYLPHFLSWVVVGG 149 Query: 121 ILFDFFSLSGMFNIILGWFGAEPVLAMQESAYFRPVYVLASIWKDAGWGTVVYMAAISAI 180 I+F+ S G+ N + WFG EP+L MQE YFR + VL+SIWK+ GWGT++Y+AAI+ I Sbjct: 150 IVFELLSNQGVVNAVRDWFGYEPILFMQEERYFRGIVVLSSIWKEVGWGTIIYLAAIAGI 209 Query: 181 DPQLYESAMIDGASRFRQIRHIXXXXXXXXXXXXXXXXXGNFLDLGFDQVYNLLTPMTYN 240 +P LYE+A++DGASR++Q +I GNFL+LGFDQ+YNLLTPMTY+ Sbjct: 210 NPNLYEAAVMDGASRWKQTIYITLPTMFPTILVLFLLQIGNFLELGFDQIYNLLTPMTYS 269 Query: 241 VGDILDTYVFRTGIQQGQYSFATAVGLFQSVIGFIMVYTFNKLSNKVSDGGLW 293 VGDI++TYV+R G+ QGQYS TA+GLFQSVIGFI+++ FN+L+ K +D GLW Sbjct: 270 VGDIIETYVYRAGVLQGQYSMTTAIGLFQSVIGFILLWIFNRLARK-TDQGLW 321 >B1DFA0.1 B1DFA0_9BACL Binding-protein-dependent transport systems inner membrane component Length = 319 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 126/290 (43%), Positives = 193/290 (66%) Query: 4 YWPLYVMAIPGIIFLIVFKFIPLAGAVIAFKDYSVFRGFIDSPWVGFKHFETLLQHPDFL 63 Y LYVM +P I FL++F +IP+ GA IAFKD+ + +G + SPWVG +HF+ + F Sbjct: 30 YRMLYVMLLPAIAFLLIFNYIPMYGATIAFKDFWITKGIMGSPWVGLEHFQKIFDTDKFW 89 Query: 64 RVFSNTLLLGLFKLTIVFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIVGGILF 123 VF NT+ + L +L FP P++LA+++NE+R+ LK+ IQT +Y+PHF+SWV + GI+F Sbjct: 90 EVFRNTIEINLLRLVFGFPAPIILAILLNEVRQKFLKRSIQTIIYLPHFVSWVTIAGIIF 149 Query: 124 DFFSLSGMFNIILGWFGAEPVLAMQESAYFRPVYVLASIWKDAGWGTVVYMAAISAIDPQ 183 S G+ N ++ G EP+ + + FRP+ +++ IWK+AGWGT++YMAA++ ++P Sbjct: 150 SLLSTDGLVNKVVVAIGGEPINFLTNNQMFRPLLIISGIWKEAGWGTIIYMAALAGVNPN 209 Query: 184 LYESAMIDGASRFRQIRHIXXXXXXXXXXXXXXXXXGNFLDLGFDQVYNLLTPMTYNVGD 243 L+E+AM+DGA+RF+Q +I G+ + GFDQV+NL M Y GD Sbjct: 210 LFEAAMVDGANRFKQAMYITLPSLLPIISILLILNFGSMMTGGFDQVFNLYNTMVYKSGD 269 Query: 244 ILDTYVFRTGIQQGQYSFATAVGLFQSVIGFIMVYTFNKLSNKVSDGGLW 293 ++DTYV+R G+ QG+YS ATA+GLF +VI F ++ N +S K+S G++ Sbjct: 270 VIDTYVYRIGLTQGEYSMATAIGLFLNVINFTLLMIVNYVSKKLSGQGIY 319 >B1D8A6.1 B1D8A6_9BACL Binding-protein-dependent transport systems inner membrane component Length = 317 Score = 278 bits (712), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 131/288 (45%), Positives = 194/288 (67%), Gaps = 1/288 (0%) Query: 7 LYVMAIPGIIFLIVFKFIPLAGAVIAFKDYSVFRGFIDSPWVGFKHFETLLQHPDFLRVF 66 LYVM +PG+++ ++FK+ P+ G +IAF+D+ F+G S WVGF+HF L PDFL + Sbjct: 30 LYVMVLPGLLYFLIFKYAPMFGLIIAFQDFKPFKGIKGSEWVGFEHFHRLFTEPDFLNIL 89 Query: 67 SNTLLLGLFKLTIVFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIVGGILFDFF 126 +NTL+L F + FPIP++LALM+NE+R +A K+ QT +Y+PHF+SWVI+ I + Sbjct: 90 TNTLVLFGFNILFYFPIPIILALMLNELRLNAFKRLFQTLIYLPHFMSWVIIVSISYVML 149 Query: 127 SLS-GMFNIILGWFGAEPVLAMQESAYFRPVYVLASIWKDAGWGTVVYMAAISAIDPQLY 185 ++ G+ N +L G E + + +FRP Y++ IW++AGWGT++Y+AA++AIDPQLY Sbjct: 150 TMDGGIVNAVLDSLGFEKINFLLSPEWFRPTYIIQVIWREAGWGTIIYLAAMAAIDPQLY 209 Query: 186 ESAMIDGASRFRQIRHIXXXXXXXXXXXXXXXXXGNFLDLGFDQVYNLLTPMTYNVGDIL 245 E+A IDGA R QI HI G+ L+LGF+ VY LL M +V +I+ Sbjct: 210 EAARIDGAGRLHQIWHITLPAIRSVIIVLLILKIGDVLELGFEHVYLLLNSMNRDVAEII 269 Query: 246 DTYVFRTGIQQGQYSFATAVGLFQSVIGFIMVYTFNKLSNKVSDGGLW 293 DTYV+ G++QG++S++TA+GLF+SV+G IMV NKLS K G++ Sbjct: 270 DTYVYTAGLKQGKFSYSTAIGLFKSVVGLIMVVAANKLSKKFGSEGIY 317 >B3KCR9.1 B3KCR9_9BACI Binding-protein-dependent transport systems inner membrane component Length = 320 Score = 277 bits (708), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 128/291 (43%), Positives = 193/291 (66%), Gaps = 1/291 (0%) Query: 4 YWPLYVMAIPGIIFLIVFKFIPLAGAVIAFKDYSVFRGFIDSPWVGFKHFETLLQHPDFL 63 Y LY +++PG++F +FK++PL G+VIAF++Y++F G SPWVG +HF + QH DFL Sbjct: 30 YRALYFISLPGLLFFALFKYVPLMGSVIAFQNYNLFAGVTGSPWVGLEHFGRMFQHYDFL 89 Query: 64 RVFSNTLLLGLFKLTIVFPIPVLLALMINEIRKSALKKGIQTALYIPHFLSWVIVGGILF 123 ++ NTLLLG + + F P++LAL++NE+R+ KK +QT Y PHFLSWVIV GI Sbjct: 90 KILRNTLLLGTYDILFAFTAPIVLALLLNEVRQVVYKKIVQTIFYAPHFLSWVIVSGIFV 149 Query: 124 DFFS-LSGMFNIILGWFGAEPVLAMQESAYFRPVYVLASIWKDAGWGTVVYMAAISAIDP 182 S SG+ N++LGWFG EP+ + E +Y R V V + +W+D GWGT++Y+AA++ I+P Sbjct: 150 GILSPSSGIVNVVLGWFGIEPIYFLGEDSYIRTVLVGSGLWRDVGWGTIIYLAALAGINP 209 Query: 183 QLYESAMIDGASRFRQIRHIXXXXXXXXXXXXXXXXXGNFLDLGFDQVYNLLTPMTYNVG 242 LYE++ IDGA R+RQ I G+F+D GF++V+ P+ Sbjct: 210 DLYEASEIDGAGRWRQTLSITLPALLPVITILFLLKIGDFMDYGFERVFVFQNPLNIQSS 269 Query: 243 DILDTYVFRTGIQQGQYSFATAVGLFQSVIGFIMVYTFNKLSNKVSDGGLW 293 +ILDTY+++ G+QQ QYS+ATA+G+F+SV+G ++ N +S K + L+ Sbjct: 270 EILDTYIYKAGLQQLQYSYATAIGVFKSVVGLFLIAIANTISKKATGESLY 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.146 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,587,104,450 Number of extensions: 61639875 Number of successful extensions: 205958 Number of sequences better than 1.0e-05: 3266 Number of HSP's gapped: 204236 Number of HSP's successfully gapped: 3267 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)