BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0020.p (619 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGU0.1 B1DGU0_9BACL Histidine kinase internal region 896 0.0 C0D692.1 C0D692_9CLOT Putative uncharacterized protein 231 2e-58 Q5WBZ1.1 Q5WBZ1_BACSK Two-component sensor histidine kinase 196 7e-48 C0C1J1.1 C0C1J1_9CLOT Putative uncharacterized protein 191 2e-46 A9KIC4.1 A9KIC4_CLOPH Multi-sensor signal transduction histidine... 183 5e-44 >B1DGU0.1 B1DGU0_9BACL Histidine kinase internal region Length = 605 Score = 896 bits (2315), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/603 (71%), Positives = 508/603 (84%) Query: 1 MEQKHWTRRIVVRIRNMKLQNKLMLGYVLVCVIPLLSVSAFIFQQSAKGLEDSSQEFASL 60 ME++ W R+ VV IRNM+ QNKLM GY+L CVIPLL VSA IF +SA+GLE SSQEFASL Sbjct: 1 MERRGWLRKAVVLIRNMRFQNKLMAGYLLACVIPLLGVSALIFHKSAEGLEQSSQEFASL 60 Query: 61 YTSQIRTTLNEFLKEYEEVTKSVLVDNDIIYSLGEAENLPFDKLIDQRLAVQRLLMRVAL 120 YTSQIRTTLNEF+ EY++VTKSVLVD+++I LGE + L D+LI QR+A+QRLLMR+ L Sbjct: 61 YTSQIRTTLNEFIDEYDKVTKSVLVDSEVISKLGEEQTLSMDELIIQRVAIQRLLMRITL 120 Query: 121 LKSEMSSVMLVSRDDSVYQFSTSTNKVNENALLAQEWYTGLRDSNETFFITGLHDRSYYE 180 LK + + +L+S++++VYQ++T+T++VNE LL+++WY LR S +TFFITGLHDRSYYE Sbjct: 121 LKPAIGTAILISKENNVYQYTTTTSRVNEGMLLSRDWYKQLRSSEDTFFITGLHDRSYYE 180 Query: 181 DKGEGAVVTAGRVLFSSDGAYAGMLLIDLDPFTLLQLDHEFILARDKYGISVVITNRQQQ 240 DKG GAVVT GRVLFSSDGAYAGMLLIDLDPF LLQLD +FILARDKYGISV+I+NR+ + Sbjct: 181 DKGAGAVVTVGRVLFSSDGAYAGMLLIDLDPFALLQLDPDFILARDKYGISVIISNREHE 240 Query: 241 IVYHSDAASGQLSWTQVLESGEEYIQDKGDKDHIVLLGSTSQGELFIRTDIPRSRLLQKI 300 IVYHSDAASG+LSW QVL+SGE YIQD I+L G+TS+ +LFI+T+IPRS+LLQKI Sbjct: 241 IVYHSDAASGRLSWEQVLDSGERYIQDDSSHGKIILTGTTSREKLFIKTEIPRSKLLQKI 300 Query: 301 NQIKVMTVIVILMSCLIITLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQANDEI 360 NQIK MTVI ILMSCL+ITL S GLS+TIT+PIKALRR MKQAE GQYL IE+ Q+NDEI Sbjct: 301 NQIKAMTVIAILMSCLVITLISLGLSYTITKPIKALRRRMKQAETGQYLPIERVQSNDEI 360 Query: 361 GSLVHSYNKMITTIRTLIEDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKD 420 GSLV SYNKMITTI TLIEDVYI EIK+RQAK ALQNQINPHMLYNTLESIRMK+L+KD Sbjct: 361 GSLVRSYNKMITTIGTLIEDVYIAEIKRRQAKLAALQNQINPHMLYNTLESIRMKSLMKD 420 Query: 421 DYETADMIKILARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEM 480 D ETADMIKILARMFRL L KEG+ HSV+HELEYTANYLQLQNIRFD+ F L IK+P EM Sbjct: 421 DEETADMIKILARMFRLTLGKEGRHHSVRHELEYTANYLQLQNIRFDDSFHLHIKVPDEM 480 Query: 481 LECSIIPLVFQPIVENSINHGFENYSRIMDIVIEGNWTEQGEMLFRITDNGVGMSLDKQS 540 L CSIIPLVFQPIVENSINHGFE+YSR ++I IEG WTE+GE+L RITD+G G+S K Sbjct: 481 LSCSIIPLVFQPIVENSINHGFEDYSRPLNIEIEGIWTEEGEILIRITDDGAGISAGKHK 540 Query: 541 ELRALLDGADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEI 600 EL LL+ A + K++L +GLGL+NIAERIKLHYG+RYYLTI P +G +EI Sbjct: 541 ELLHLLEEAGAGKHRLADAELPENEGLGLKNIAERIKLHYGERYYLTIRPGQDRGTIVEI 600 Query: 601 LIP 603 LIP Sbjct: 601 LIP 603 >C0D692.1 C0D692_9CLOT Putative uncharacterized protein Length = 589 Score = 231 bits (589), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 161/608 (26%), Positives = 298/608 (49%), Gaps = 44/608 (7%) Query: 16 NMKLQNKLMLGYVLVCVIPLLSVSAFIFQQSAKGLEDSSQEFASLYTSQIRTTLNEFLKE 75 +M+L+ KL++ Y+L VIP+L + I + S K + ++++A + +I + F++ Sbjct: 14 DMRLERKLLVSYILFAVIPILVIGTVICRVSFKIIMRQTRQYADMMIERINNDADAFVEN 73 Query: 76 YEEVTKSVLVDNDIIYSLGEAENLPFDKLIDQRLAVQRLLMRVALL-KSEMSSVMLVSRD 134 E V + +I L A+ F +L D + + + L+ + + K + S + + Sbjct: 74 IERQAYVVYTEPEIQDILEHADRYTFSQLQDGKEMIHKRLIGMWVTEKYIVGSYLCGAEG 133 Query: 135 DSVYQFSTSTNKVNENALLAQE-WYTGLRDSNETFFITGLHDRSYYEDKGEGAVVTAGRV 193 +S Y N ++ + + E WY + + +TG+H Y D +++ Sbjct: 134 NSYYTNINERNDIDPAQVFSGESWYATITEGARKGVLTGVHRDDKYSDSSSLQLISYVHA 193 Query: 194 LFS-SDGAYAGMLLIDLDPFTLLQLDHEFILAR-DKYGISVVITNRQQQIVYHSDAASGQ 251 + S G Y G L+ID+D L E + R + G + I + + ++Y + Sbjct: 194 VNSIGTGKYLGFLVIDIDARVL----GEIVGNRAENLGGYITILDNENNVMYTT------ 243 Query: 252 LSWTQVLESGEEYIQDKGDKDHIVLLGSTSQGELF-IRTDIPRSRLLQKINQIKVMTVIV 310 ++ G ++ + S G + +R +IP SR ++I I ++T +V Sbjct: 244 ------------LPEETGHLGQVICMEHVSPGTGWTVRMEIPWSRFAKEIVNIMLLTAVV 291 Query: 311 ILMSCLIITLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQANDEIGSLVHSYNKM 370 + L+ S +S +I+ PI+ L+ +M + E G + + +DEIG L S+N M Sbjct: 292 GALCFLVFIWLSRKISRSISAPIRELQTAMARVEEGDMEVVVRADRDDEIGRLAGSFNHM 351 Query: 371 ITTIRTLIEDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKDDYETADMIKI 430 ++ I+ LIE VY+ ++ +++A+F ALQ+QINPH +YNTLE++ M A++ YE +D++ Sbjct: 352 VSNIKDLIEKVYLTQLGKKEAEFNALQSQINPHFMYNTLETVNMMAILDGSYEISDILTA 411 Query: 431 LARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEMLECSIIPLVF 490 ++ R+ L+++ + ELEY NYL + IR +F E+ E +C I L Sbjct: 412 FGKILRVNLDQKHNIIPLGRELEYIRNYLMIVQIRDPELFTFELNASSEAEKCLIPKLTL 471 Query: 491 QPIVENSINHGFENYSRIMDIVIEGNWTEQGEMLFRITDNGVGMSLDKQSELRALLDGAD 550 QPIVENSI HGF+ + + E+G ++ + D+G GM+ +K +R L+ Sbjct: 472 QPIVENSILHGFKEKMSRRRLTVNAA-VERGRLVITVEDDGKGMTGEKLEYVRQSLE--- 527 Query: 551 SHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEILIPGF----- 605 LE + G + +GL N++ER +L++G+ + + I G + + Sbjct: 528 -----LEESLGRS-ASIGLNNVSERCQLYFGESFSMEIHSRRGTGTRVALNAAAIYGEGE 581 Query: 606 --DASGHF 611 D SGH+ Sbjct: 582 ENDTSGHY 589 >Q5WBZ1.1 Q5WBZ1_BACSK Two-component sensor histidine kinase Length = 577 Score = 196 bits (497), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 150/597 (25%), Positives = 285/597 (47%), Gaps = 32/597 (5%) Query: 17 MKLQNKLMLGYVLVCVIPLLSVSAFIFQQSAKGLEDSSQEFASLYTSQIRTTLNEFLKEY 76 M L+ K++L ++ + ++PL + + +K LED + + Q+ +N ++ E Sbjct: 1 MSLRPKIILLFLSIVLVPLNLLGMITYAYFSKTLEDQTYHYTVQVIGQVNQNVNAYIDEM 60 Query: 77 EEVTKSVLVDNDIIYSLGEAENL--PFDKLIDQRLAVQRLLMRVALLKSEMSSVMLVSRD 134 ++ L D DI+ L ++ + ++ + L ++ + E+S + +++RD Sbjct: 61 HRLSLLPLYDRDILAILKNRSDVHHSYYPQTEEFERMSSFLSTLSYNRQELSGIHIITRD 120 Query: 135 DSVYQFSTSTNKVNENALLAQEWYTGLRDSNETFFITGLHDRSYYEDKGEGAVVTAGRVL 194 S++ S V+ A +W + N + H SY E +V + GR+L Sbjct: 121 GSLFSDLGSPRTVHRLAEGTSDWQEAIEAGNGASLLLPTHSPSYVLG-AEKSVFSVGRLL 179 Query: 195 FSSDGAYA-GMLLIDLDPFTLLQLDHEFILARDKYGISVVITNRQQQIVYHSDAA----- 248 DG GM+ +D++ + L + L+ D S+ + +R + +Y + A Sbjct: 180 RDPDGFRPLGMIKVDIELSFIESLLEDVDLSEDA---SITMIDRNGEPIYETGKAIYPLL 236 Query: 249 --SGQLSWTQVLESGEEYIQDKGDKDHIVLLGSTSQGELFIRTDIPRSRLLQKINQIKVM 306 G+ W Q+ E E I G D++ ++ + S G++ +P ++ +++ Sbjct: 237 KGKGEDVW-QLAE--RESIAVDG-VDYLPVIRNDSSGDVQTIVFLPEHHIVGASQSLRLF 292 Query: 307 TVIVILMSCLIITLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQANDEIGSLVHS 366 T+ ++L ++ + + ++T+PI LR+ M AE G++ + DE+G L Sbjct: 293 TIGLMLAVSAVVIMLALVAERSLTKPIFELRQVMALAEKGEFHHRMNRSSKDELGQLAIG 352 Query: 367 YNKMITTIRTLIEDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKDDYETAD 426 +N M+ I LI VY EI+++ A+ ALQ Q+NPH +YNTLE I M A+ K YE +D Sbjct: 353 FNHMMKQINELIAKVYQTEIREKDAEIKALQTQMNPHFIYNTLEHINMMAITKQAYELSD 412 Query: 427 MIKILARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEMLECSII 486 M+ L R+ R ++++ + ++ EL + +Y+ +Q R + I +P M SI Sbjct: 413 MVASLGRLIRYSIDQKTRFVPLREELAFVESYITIQQKRLEGKVTFSIAVPEAMRSLSIP 472 Query: 487 PLVFQPIVENSINHGFENYSRIMDIVIEGNWTEQGEMLFRITDNGVGMSLDKQSELRALL 546 L+ QP++EN+I HG ++ +I I+G W + + D+G GM KQ + + Sbjct: 473 KLILQPLIENAIEHGHQHGEWPGEISIKG-WQDDAYSYIEVRDSGQGM---KQETIERM- 527 Query: 547 DGADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEILIP 603 K + E G+ + L NI+ER+ L G++ L I +G + + +P Sbjct: 528 ------KEEPERGAGNQ---VALGNISERLLLLLGEQARLMIDSELGRGTKVTVRLP 575 >C0C1J1.1 C0C1J1_9CLOT Putative uncharacterized protein Length = 601 Score = 191 bits (485), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 156/609 (25%), Positives = 297/609 (48%), Gaps = 29/609 (4%) Query: 11 VVRIRNMKLQNKLMLGYVLV--CVIPLLSVSAFIF-QQSAKG-LEDSSQEFASLYTSQIR 66 +++ +++K++NKL +G++LV ++ LL + F +QS + + DS QE + I Sbjct: 3 IIKYKDLKMKNKLFIGFILVIAVLVSLLGYFSMTFSEQSIRTQMLDSIQETMNQMGENIE 62 Query: 67 TTLNEFLKEYEEVTKSVLVDNDIIYSLGEAENLPFDKLIDQRLAVQRLLMRVALLKSEMS 126 + + L +V+ V ++ ++ L E + ++ + ++++L + Sbjct: 63 GEIQKVL----DVSAQVCLNEELCTVLQEEKPGTIEEYYSKDKTMRQILNESYAAYFSVE 118 Query: 127 SVMLVSRDDSVYQFSTSTNKVNENA---LLAQEWYTGLRDSNETFFITGLHDRSYYEDKG 183 V + S + SVY + NK++ A W+ ++ S I +D S Y G Sbjct: 119 DVFVCSYNGSVY--GSDKNKLSLTADYDFTRTGWFADMKQSGAGVSILSQYDSSSYISTG 176 Query: 184 EGAVVTA--GRVLFSSDGAYAGMLLIDLDPFTLLQLDHEFILARDKYGISVVITNRQQQI 241 E + + ++ G G L++ ++ L + + ++Y ++I N ++ I Sbjct: 177 EPQKLFSIVKKIYNYRSGKEIGCLIVHMNSDILGNVLQS--VNSNEYQQCLIIDNNKK-I 233 Query: 242 VYHSDA-------ASGQLSWTQVLESGEEYIQDKGDKDHIVLLGSTSQGELFIRTDIPRS 294 +YH D+ ++S +E+G Y++ +K ++V S G I + Sbjct: 234 IYHPDSDYIGTQYREARVSELLTMENG--YMESLSEKGYLVFSTSAVTGWSVISV-VDNK 290 Query: 295 RLLQKINQIKVMTVIVILMSCLIITLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKE 354 ++ IN++++ ++ + ++ F+ +S TI+ P+ L+R M Q G + Sbjct: 291 YIMDSINRLRLFLILTAVFCVVLSVYFASYISKTISDPVARLQRKMYQVGEGDFDIRAAT 350 Query: 355 QANDEIGSLVHSYNKMITTIRTLIEDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRM 414 A+DEIG L S++KM+ + LIE+VY EI Q++A+ ALQ QINPH LYNTL++I M Sbjct: 351 GASDEIGQLTLSFDKMVERTKQLIENVYQSEIYQKEAELNALQAQINPHFLYNTLQTIDM 410 Query: 415 KALIKDDYETADMIKILARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMFKLEI 474 A + E +D + L+++FR +N+ + V+ E+ + NY+ +Q +RF ++ Sbjct: 411 MAEEEGADEISDACQALSKIFRYSINRGQEFVHVQDEIVHIENYMLIQKLRFGGKLEVRY 470 Query: 475 KIPGEMLECSIIPLVFQPIVENSINHGFENYSRIMDIVIEGNWTEQGEMLFRITDNGVGM 534 I E E I+ L+ QP+VEN++ HG EN + I + ++ + + DNG G+ Sbjct: 471 DIQNECRELYIVKLLIQPMVENAVVHGMENVLDKCYVTIRV-YRKEDCLYAEVEDNGTGI 529 Query: 535 SLDKQSELRALLDGADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQ 594 + + EL L + K E + + +GL+N RIKL++G Y +TI Sbjct: 530 APGQLMELNEKLARSAEQKMVHEVDYVKNHRSIGLENTNARIKLYFGQEYGITITSEEGT 589 Query: 595 GVTIEILIP 603 G + I +P Sbjct: 590 GTKVCIKLP 598 >A9KIC4.1 A9KIC4_CLOPH Multi-sensor signal transduction histidine kinase Length = 585 Score = 183 bits (465), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 149/561 (26%), Positives = 278/561 (49%), Gaps = 32/561 (5%) Query: 53 SSQEFASLYTSQIRTTLNEFLKEYEEVTKSVLVDNDIIYSLGEAENLPFDKLIDQRLAVQ 112 +S E+ S +Q+ + ++ ++ E +++ L+D+D+ L N +++L ++ A + Sbjct: 42 NSMEYTSELINQMNSDVDSYIGYMENISQLALLDSDVHTFL----NSDYEELDSRQFAKE 97 Query: 113 RLLMRVAL---LKSEMSSVMLVSRDDSVYQFSTSTNKVNENALLAQ-EWYTGLRDSNETF 168 R++ + L+S+++++ ++ + Y + +++N + Q EWY N Sbjct: 98 RVMNQFGTILDLRSDITNLAIIGTNGR-YILNRGEDEINPYLDVKQKEWYKKAIAGNGKI 156 Query: 169 FITGLHDRSYYEDKGEGAVVTAGRVLFSSDGAYAGMLLIDLDPFTLLQLDHEFILARDKY 228 +I+ H ++ K + V + + G LL+DL+ + L + L Y Sbjct: 157 YISSSHVQNIVNGKYQWVVSLSRAIKDKESDEMIGALLVDLNYNVINNLCKKINLGYKGY 216 Query: 229 GISV-----VITNRQQQIVYHSDAASGQLSWTQVLESGEEYIQDKG-DKDHIVLLGSTSQ 282 + +I + +QQ++Y+ L +V E E Y +G D+ V+ S Sbjct: 217 IFLLDEEGNIIYHPKQQLIYNGLINEKTL---EVKECKESYFLTEGTDRKLYVISRSEKT 273 Query: 283 GELFIRTDIPRSRLLQKINQIKVMTVIVILMSCLIITLFSFGLSFTITRPIKALRRSMKQ 342 G + LQ+ Q + + +I + LI L S +S IT+PIK LR SMK+ Sbjct: 274 GWTAVGVANVSELFLQR-EQTQRLYLISTVAILLIAMLLSILISRAITKPIKKLRDSMKE 332 Query: 343 AEVGQYLAIEKEQANDEIGSLVHSYNKMITTIRTLIEDVYIGEIKQRQAKFIALQNQINP 402 E G + + +DEI +L SYN MI T+ L+E + ++R+++ ALQ QINP Sbjct: 333 VEKGNFHEKVETSGSDEIANLGKSYNIMIQTVDELMEARVKDQEQKRKSELRALQAQINP 392 Query: 403 HMLYNTLESIRMKALIKDDYETADMIKILARMFRLVLNKEGKQHSVKHELEYTANYLQLQ 462 H LYNTL+SI A + E M LA++FR ++ E ++ +++ E++Y NYL +Q Sbjct: 393 HFLYNTLDSIVWMAEEGKNQEVVTMTASLAKLFRQSISNEAEEFTLRQEVDYVRNYLVIQ 452 Query: 463 NIRFDNMFKLEIKIPGEMLECSIIPLVFQPIVENSINHGFENYSRIMDIVIEGNWTEQGE 522 +R+ +I++P +L +I L+ QP+VEN++ HG + + I ++G TE Sbjct: 453 KMRYKEQLNYDIQVPESILNYYVIKLILQPLVENALYHGVKYKDGVGTIFVDGYETE-ST 511 Query: 523 MLFRITDNGVGMSLDKQSELRALLDGADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGD 582 ++ ++ D+G+GM + EL SH ++ + T + + ++NI RI+LHYG Sbjct: 512 VVLQVIDDGIGMD---EEEL--------SHLFEKRKSTHKTSR-VAIRNIHNRIQLHYGK 559 Query: 583 RYYLTIVPMGAQGVTIEILIP 603 + L+ G + I +P Sbjct: 560 EFGLSFESKKGYGTKVMITLP 580 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,344,916,941 Number of extensions: 136247126 Number of successful extensions: 395182 Number of sequences better than 1.0e-05: 2121 Number of HSP's gapped: 392262 Number of HSP's successfully gapped: 2121 Length of query: 619 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 477 Effective length of database: 1,722,912,073 Effective search space: 821829058821 Effective search space used: 821829058821 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)