BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0019.p (241 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A0RCQ2.1 A0RCQ2_BACAH Possible permease 303 1e-80 B3ZPE3.1 B3ZPE3_BACCE Cdd2 303 1e-80 B3KJI6.1 B3KJI6_9BACI Putative MrsE protein 303 1e-80 C2NG87.1 C2NG87_BACCE Permease 301 3e-80 Q4MHS9.1 Q4MHS9_BACCE Cdd2 294 5e-78 >A0RCQ2.1 A0RCQ2_BACAH Possible permease Length = 243 Score = 303 bits (775), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 149/240 (62%), Positives = 187/240 (77%) Query: 1 MLRSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGE 60 M RS++AEW KLRHSR+GLV+A+LP+IS+LIG NFY NQ ALQNGWYSLWTQVSLFYG Sbjct: 1 MKRSISAEWLKLRHSRVGLVIAALPIISMLIGSANFYLNQGALQNGWYSLWTQVSLFYGV 60 Query: 61 FFLPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILY 120 FFLPILIAICC+++CRLEH+NRNWNMI+ +PV + ++FLAKL IV ILI AQ F+ LY Sbjct: 61 FFLPILIAICCSYICRLEHLNRNWNMIMTSPVSVANVFLAKLIIVSILILFAQGLFMGLY 120 Query: 121 WTAGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXX 180 W G + S+ FP+E I WT RGW AS++I+ALQLGLS+RIRSFATPIGISLC VFI Sbjct: 121 WLIGTLFSLPGSFPVETIEWTIRGWYASLSISALQLGLSLRIRSFATPIGISLCGVFIGL 180 Query: 181 XXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 FP+SLL IGM V++Q LT ++LLF+++N ++++ + SIRRL+ RDV Sbjct: 181 GMYIGKFGMLFPFSLLNIGMSVMSQDKLTNMQSLLFWVINMAFVIIFASRSIRRLKNRDV 240 >B3ZPE3.1 B3ZPE3_BACCE Cdd2 Length = 243 Score = 303 bits (775), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 149/240 (62%), Positives = 187/240 (77%) Query: 1 MLRSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGE 60 M RS++AEW KLRHSR+GLV+A+LP+IS+LIG NFY NQ ALQNGWYSLWTQVSLFYG Sbjct: 1 MKRSISAEWLKLRHSRVGLVIAALPIISMLIGSANFYLNQGALQNGWYSLWTQVSLFYGV 60 Query: 61 FFLPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILY 120 FFLPILIAICC+++CRLEH+NRNWNMI+ +PV + ++FLAKL IV ILI AQ F+ LY Sbjct: 61 FFLPILIAICCSYICRLEHLNRNWNMIMTSPVSVANVFLAKLIIVSILILFAQGLFMGLY 120 Query: 121 WTAGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXX 180 W G + S+ FP+E I WT RGW AS++I+ALQLGLS+RIRSFATPIGISLC VFI Sbjct: 121 WLIGTLFSLPGSFPVETIEWTIRGWYASLSISALQLGLSLRIRSFATPIGISLCGVFIGL 180 Query: 181 XXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 FP+SLL IGM V++Q LT ++LLF+++N ++++ + SIRRL+ RDV Sbjct: 181 GMYIGKFGMLFPFSLLNIGMSVMSQDKLTNMQSLLFWVINMAFVIIFASRSIRRLKNRDV 240 >B3KJI6.1 B3KJI6_9BACI Putative MrsE protein Length = 243 Score = 303 bits (775), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 146/240 (60%), Positives = 190/240 (79%) Query: 1 MLRSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGE 60 M RS++AEW KLRHSR+GL++A LP++SLLIGC N+YFNQ+ALQNGWYSLWTQV+LFYG Sbjct: 1 MKRSISAEWLKLRHSRIGLIIAVLPILSLLIGCANYYFNQDALQNGWYSLWTQVALFYGV 60 Query: 61 FFLPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILY 120 FFLPILI+ICC+++ RLEH+NRNWN+++ +PV I S+FLAK +VG+LI AQV F+ LY Sbjct: 61 FFLPILISICCSYIGRLEHLNRNWNIVMTSPVSIASVFLAKWIVVGVLILFAQVLFIGLY 120 Query: 121 WTAGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXX 180 W AG + S+ PFP+EII W RGW A ++I ALQLGLS+RIRSFATPIGISLCAVFI Sbjct: 121 WLAGTLFSLPGPFPVEIIGWIIRGWYACMSITALQLGLSLRIRSFATPIGISLCAVFIGL 180 Query: 181 XXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 FP+SLL IGM V++Q LT +N+LF+I+N +I++ + +IRRL+ +D+ Sbjct: 181 GMYIAKLGLFFPFSLLNIGMSVMSQDPLTGMQNILFWIINLAFIIIFASMTIRRLKNKDI 240 >C2NG87.1 C2NG87_BACCE Permease Length = 243 Score = 301 bits (771), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 148/240 (61%), Positives = 186/240 (77%) Query: 1 MLRSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGE 60 M RS++AEW KLRHSR+GLV+A+LP+IS+LIG NFY NQ ALQNGWYSLWTQVSLFYG Sbjct: 1 MKRSISAEWLKLRHSRVGLVIAALPIISMLIGSANFYLNQGALQNGWYSLWTQVSLFYGV 60 Query: 61 FFLPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILY 120 FFLPILIAICC+++CRLEH+NRNWNMI+ +PV + ++FLAKL IV ILI AQ F+ Y Sbjct: 61 FFLPILIAICCSYICRLEHLNRNWNMIMTSPVSVANVFLAKLIIVSILILFAQGLFMGFY 120 Query: 121 WTAGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXX 180 W G + S+ FP+E I WT RGW AS++I+ALQLGLS+RIRSFATPIGISLC VFI Sbjct: 121 WLIGTLFSLPGSFPVETIEWTIRGWYASLSISALQLGLSLRIRSFATPIGISLCGVFIGL 180 Query: 181 XXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 FP+SLL IGM V++Q LT ++LLF+++N ++++ + SIRRL+ RDV Sbjct: 181 GMYIGKFGMLFPFSLLNIGMSVMSQDKLTNMQSLLFWVINMAFVIIFASRSIRRLKNRDV 240 >Q4MHS9.1 Q4MHS9_BACCE Cdd2 Length = 243 Score = 294 bits (752), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 145/240 (60%), Positives = 182/240 (75%) Query: 1 MLRSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGE 60 M RS+ AEW KLRHSR+GLV+A LP+IS+LIG N+ NQ ALQNGW+SLWTQVSLFYG Sbjct: 1 MKRSIFAEWLKLRHSRIGLVIAVLPIISMLIGSANYNLNQGALQNGWFSLWTQVSLFYGV 60 Query: 61 FFLPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILY 120 FFLPILIAICC+++CRLEH+NRNWNMI+ +P+ + ++FLAKL IV ILI AQ F+ LY Sbjct: 61 FFLPILIAICCSYICRLEHLNRNWNMIMTSPISVANVFLAKLIIVSILILFAQCLFIGLY 120 Query: 121 WTAGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXX 180 W G + S+ PFP+E I WT RGW ASI+I++LQLGLS+RIRSFATPIGISLC VFI Sbjct: 121 WLIGTLFSLPGPFPLETIEWTLRGWYASISISSLQLGLSLRIRSFATPIGISLCGVFIGL 180 Query: 181 XXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 FP+SLL IGM V+ Q LT + LF+++N +I++ + SIRRL+ RD+ Sbjct: 181 GMYIGKFGMLFPFSLLNIGMSVMNQDKLTNMQGFLFWMINMAFIIIFASRSIRRLKNRDI 240 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.333 0.145 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,298,284,324 Number of extensions: 48295464 Number of successful extensions: 219494 Number of sequences better than 1.0e-05: 94 Number of HSP's gapped: 219422 Number of HSP's successfully gapped: 94 Length of query: 241 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 109 Effective length of database: 1,817,122,223 Effective search space: 198066322307 Effective search space used: 198066322307 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 129 (54.3 bits)