BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0014.p (419 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q187K2.1 Q187K2_CLOD6 Putative membrane protein 251 7e-65 C1UE37.1 C1UE37_9ACTN Putative uncharacterized protein 196 3e-48 B6GBF5.1 B6GBF5_9ACTN Putative uncharacterized protein 181 1e-43 B1B7Q0.1 B1B7Q0_CLOBO Putative membrane protein 166 5e-39 A0PYE0.1 A0PYE0_CLONN Membrane protein, putative 163 3e-38 >Q187K2.1 Q187K2_CLOD6 Putative membrane protein Length = 413 Score = 251 bits (642), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 146/421 (34%), Positives = 232/421 (55%), Gaps = 10/421 (2%) Query: 1 MNNLFKYEFMKIAKKRMNIXXXX-XXXXXXXXXXIMPVKNYIALERDGSQVNGTAAIRLE 59 M+ L K+E KI K++ I +MP++ A + G + G AI L+ Sbjct: 1 MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEK-ANDESGKEYRGLQAIELK 59 Query: 60 KEYAKELEGTLTEERVAKDIKAYQALFSNPENVVKD-GDQVNLKDDIYARSVLPHFAYFK 118 K+ +K +G +T+++V I+ YQ +F+NP N+ D G N K DIY + P+ A++ Sbjct: 60 KQQSKLNDGYITDKKVENSIREYQKMFNNPNNITTDEGGFSNFKPDIYWKFFYPNKAFYN 119 Query: 119 MIDEAFVKPQVTDNGLTALRDMRLNKDTAGFYQARADKVKSYLDMDYSDWKYSEQEKQFW 178 I E++ P D ++ M L +D FY+ R +KV + L+M+Y D Y+++EK FW Sbjct: 120 FIIESYSNPN--DYNPEIIKSMPL-EDGVNFYKFRNEKVNTILNMEYPDKNYTQKEKDFW 176 Query: 179 NTHNSQVETPYEYGYHAGWKSLFQCFEXXXXXXXXXXXXXXPVFAGEYQSGADSVILSTR 238 + N +E PY GY+ GW + VF+GEYQSGADSV+L+++ Sbjct: 177 LSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIMAVCISIASVFSGEYQSGADSVVLTSK 236 Query: 239 YGKSKLIQAKISAAFLFALLVFTVNIVLAVGIQLYAFGTGGWNLPLQILNVTVPYNLSML 298 YGK+K+I AKI ++FLF ++F +N ++++GI L +FG GWNLPLQ+ ++PY + L Sbjct: 237 YGKTKVITAKIISSFLFGTIIFVINTIVSIGILLLSFGIDGWNLPLQLTTSSIPYPYTFL 296 Query: 299 EAAVVCVSILYLVLLGMVAVTLLLSAKMKTAFXXXXXXXXXXXXXXXXGLSGTNGILNHI 358 +A ++ V I+Y++L+ +++ TLLLS+ MK+ F S TN + N I Sbjct: 297 QAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITNIILLFVPIFFKYSETNKLYNQI 356 Query: 359 LMLLPYMAVQPVFPADYTSYFSYPLPGLTLSIITVRIVLYVVICSVCLPFVGRVFKRHQV 418 L +LP A+ +T Y Y L +++ T+ I +V+ V LPFV FK+HQV Sbjct: 357 LRILPSNAIS----FSFTDYIDYKFGNLIVTLPTMIIFTCLVLTVVFLPFVKNNFKKHQV 412 Query: 419 Q 419 Sbjct: 413 N 413 >C1UE37.1 C1UE37_9ACTN Putative uncharacterized protein Length = 421 Score = 196 bits (499), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 140/431 (32%), Positives = 210/431 (48%), Gaps = 24/431 (5%) Query: 1 MNNLFKYEFMKIAKKRMNIXXXXXXXXXXXXXXIMPVKNYIALERDGSQVNGTAAIRLEK 60 M +L ++E K+ +R ++ + + G +NGTAAI +K Sbjct: 1 MLDLVRFELKKLLVRRTSLIACAGILVMLCGIMTLNIVQTKTASDTGEVLNGTAAIAHQK 60 Query: 61 EYAKELEGTLTEERVAKDIKAYQAL-FSNPENVVKDGDQVNLKDDIYARSVLPHFAY--- 116 E A EG LT ERV ++ AYQAL FS V D L D+ A L AY Sbjct: 61 EKAAAHEGVLTVERVQDEVVAYQALAFSK----VDPADVAGLSDE--AAYALMIQAYDED 114 Query: 117 -FKMIDEAFVKPQVTDNGLTALR----DMRLNKDTA-GFYQARADKVKSYLDMDYSD-WK 169 F+ I +++ ++ + AL +L+ A GFY+A A K++ LD D W Sbjct: 115 EFRAIYDSYYAYLLSPWKVGALEPYQVAAQLDDGAADGFYEAVAGKLQRTLDNDMGGTWT 174 Query: 170 YSEQEKQFWNTHNSQVETPYEYGYHAGWKSLFQCFEXXXXXXXXXXXXXXPVFAGEYQSG 229 YS+ E+ +W V P YGY GW + C PVF+GEYQ Sbjct: 175 YSDAERAYWTEKQGGVGEPLAYGYAGGWSDVLACLGFFAFAMVAVCIALTPVFSGEYQDR 234 Query: 230 ADSVILSTRYGKSKLIQAKISAAFLFALLVFTVNIVLAVGIQLYAFGTGGWNLPLQILNV 289 D+V+LS+RYG+SKL+ AKI AA FA F + ++ +G L FG G +LP+Q+L++ Sbjct: 235 TDAVLLSSRYGRSKLVAAKIVAALAFATAYFALATLIIMGAALAFFGAEGGDLPVQVLSL 294 Query: 290 TVPYNLSMLEAAVVCVSILYLVLLGMVAVTLLLSAKMKTAFXXXXXXXXXXXXXXXXGLS 349 ++PY+L+M EA V I YL+ LG+ +TLLLS+K+++ S Sbjct: 295 SIPYDLTMAEATWTAVGIAYLMTLGLAGLTLLLSSKLRSQLAIFAACAALIFLTGMIP-S 353 Query: 350 GTNGILNHILMLLPYMAV--QPVFPADYTSYFSYPLPGLTLSIITVRIVLYVVICSVCLP 407 G N +L H L L P + Q +F S SYP+ + ++ + +Y ++ + C+P Sbjct: 354 GDNAVLLHALYLFPINGLNDQVLF----NSLVSYPVGSFAIDLVGLLACVYALVLAACVP 409 Query: 408 FVGRVFKRHQV 418 R F+RHQV Sbjct: 410 LAARAFRRHQV 420 >B6GBF5.1 B6GBF5_9ACTN Putative uncharacterized protein Length = 422 Score = 181 bits (458), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 121/424 (28%), Positives = 190/424 (44%), Gaps = 9/424 (2%) Query: 1 MNNLFKYEFMKIAKKRMNIXXXXXXXXXXXXXXIMPVKNYIALERDGSQVNGTAAIRLEK 60 M +L K+E K+ +R+ + + V G ++G AAI + Sbjct: 1 MRSLIKFELKKMLTRRVAVAVNLGVIAMLLVVMTLNVVQAKTEGNIGEILSGPAAIAHRR 60 Query: 61 EYAKELEGTLTEERVAKDIKAYQALFS---NPENVVKDGDQVNLKDDIYARSVLPHFAYF 117 E A+ G LT ERVA DI YQ + +PE + + + A + Sbjct: 61 EVAEVHAGELTPERVAADIAHYQDIAYEKLDPEELAEMSGAAAYEATADAYDEATRLELY 120 Query: 118 KMIDEAFVKPQVTDNGLTALRDMRLNKDTA-GFYQARADKVKSYLD--MDYSDWKYSEQE 174 +KP R+ + A FY A AD +++ LD MD W YS+ E Sbjct: 121 DPYYVTILKPWAVSGQEPYQYASRVTPEMALDFYGALADSLQTTLDEGMD-GRWVYSDAE 179 Query: 175 KQFWNTHNSQVETPYEYGYHAGWKSLFQCFEXXXXXXXXXXXXXXPVFAGEYQSGADSVI 234 + +W + V P YG+ GW ++ C P+FAGEY+ G D+V+ Sbjct: 180 RAYWTEKEAGVSEPLAYGWAGGWDNILDCGAFLVLVAFAACVTVTPLFAGEYRDGTDAVL 239 Query: 235 LSTRYGKSKLIQAKISAAFLFALLVFTVNIVLAVGIQLYAFGTGGWNLPLQILNVTVPYN 294 LS RYG+ +L+ AK+ A L+ +F V + G+ L +G G LP+Q + PY+ Sbjct: 240 LSARYGRGRLVAAKLLVALLYTTALFVVGAAIICGVSLVFYGAEGAALPIQNYALISPYS 299 Query: 295 LSMLEAAVVCVSILYLVLLGMVAVTLLLSAKMKTAFXXXXXXXXXXXXXXXXGLSGTNGI 354 L+M++A + + I YL+ +GMVA TL LSA + + +G +G+ Sbjct: 300 LTMVQACAMFIGIFYLITIGMVAFTLALSACLSSTLAIIVTDVAILFVSGMLP-TGGSGL 358 Query: 355 LNHILMLLPYMAVQPVFPADYTSYFSYPLPGLTLSIITVRIVLYVVICSVCLPFVGRVFK 414 LNHI L P +Q F + FSYPL + + +I + ++Y + V +P + Sbjct: 359 LNHIFTLFPMGNIQSPFVV-FAELFSYPLGPIVIDLIGMAALVYCALMVVGVPVAWFAWS 417 Query: 415 RHQV 418 RHQV Sbjct: 418 RHQV 421 >B1B7Q0.1 B1B7Q0_CLOBO Putative membrane protein Length = 412 Score = 166 bits (419), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 123/422 (29%), Positives = 199/422 (47%), Gaps = 15/422 (3%) Query: 1 MNNLFKYEFMKIAKKRMNIXXXXXXXXXXXXXXIMPVKNYIALERDGSQVNGTAAIRLEK 60 M L +YE KI K++ I +K+ ++ G +NG +I L+K Sbjct: 1 MGTLIRYELRKIFKRKSTKISLGVILLWLLCLGIFTIKDQDYTDKSGKMINGLKSIELKK 60 Query: 61 EYAKELEGTLTEERVAKDIKAYQALFSNPENVVKDGDQVNLKDDIYARSVLPHFAYFKMI 120 E + EG L++E++ YQ + + +N + LK++ +A+ V + +++ Sbjct: 61 EGVTKHEGYLSKEKLTNVFNDYQKIIKDSKNYQPNSKW--LKNEAFAKYVDKN----EIL 114 Query: 121 DEAFVKPQVTDNGLTALRDMRLNKDTAGFYQARADKVKSYLDMDYSDWKYSEQEKQFWNT 180 D D + D +KD FY+ R +KVK L+ YS YS+ EK + + Sbjct: 115 DLLRQDFSYADKYNYDIIDSLHSKDIKDFYKRRDEKVKEILNKKYSHGSYSKSEKDYVIS 174 Query: 181 HNSQVETPYEYGYHAGW----KSLFQCFEXXXXXXXXXXXXXXPVFAGEYQSGADSVILS 236 N ++ P+ + Y GW + LFQ P+FA EYQ+GADSVILS Sbjct: 175 LNQKISKPFYFNYFNGWEEILRRLFQIM--AMLIALVISICVAPIFALEYQTGADSVILS 232 Query: 237 TRYGKSKLIQAKISAAFLFALLVFTVNIVLAVGIQLYAFGTGGWNLPLQILNVTVPYNLS 296 TRYGK+KLI AKI A FL A V+ I++ L+ +G GWN Q++++ Y S Sbjct: 233 TRYGKNKLIIAKILAVFLCATFVYFGLILIVTLGMLWCYGIEGWNAAFQVMSIKSVYPYS 292 Query: 297 MLEAAVVCVSILYLVLLGMVAVTLLLSAKMKTAFXXXXXXXXXXXXXXXXGLSGTNGILN 356 +LE ++ V+I YLV+L + +LLS+K+KT+ N LN Sbjct: 293 VLEVYLLGVAIGYLVILAFSSFIMLLSSKIKTS-AIMVISGLLIFVPILVRRDKENLALN 351 Query: 357 HILMLLPYMAVQPVFPADYTSYFSYPLPGLTLSIITVRIVLYVVICSVCLPFVGRVFKRH 416 ++ L P AV +++SY +Y + L + +L V+ + +P F+ H Sbjct: 352 RVMNLFPGNAVNTF--VNFSSYDTYSFKNIVLLAPQLITILSVIGICIFVPIAFYNFRNH 409 Query: 417 QV 418 +V Sbjct: 410 EV 411 >A0PYE0.1 A0PYE0_CLONN Membrane protein, putative Length = 412 Score = 163 bits (412), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 121/421 (28%), Positives = 199/421 (47%), Gaps = 13/421 (3%) Query: 1 MNNLFKYEFMKIAKKRMNIXXXXXXXXXXXXXXIMPVKNYIALERDGSQVNGTAAIRLEK 60 M L KYE KI +++ + +K+ ++ G +NG +I L+K Sbjct: 1 MGTLIKYELKKIFRRKSTKVSLVVMLLWLLCWGSIIIKDNDYTDKSGKGINGLKSIELKK 60 Query: 61 EYAKELEGTLTEERVAKDIKAYQALFSNPENVVKDGDQVNLKDDIYARSVLPHFAYFKMI 120 + EG L+++++ YQ + + +N + LK++ +A+ V H +++ Sbjct: 61 VAVTKHEGYLSKDKLTNVFNDYQKIIKDSKNYQPNSKW--LKNEAFAKYVDKH----EIL 114 Query: 121 DEAFVKPQVTDNGLTALRDMRLNKDTAGFYQARADKVKSYLDMDYSDWKYSEQEKQFWNT 180 D D + D NKD FY+ R +KVK L+ Y+ YS+ EK + + Sbjct: 115 DLLRQDFSYADKYNYDIIDSLSNKDVKDFYKRRDEKVKEILNKKYAHGSYSQSEKDYVIS 174 Query: 181 HNSQVETPYEYGYHAGWKSLFQCF--EXXXXXXXXXXXXXXPVFAGEYQSGADSVILSTR 238 N ++ P+ + Y GW+ + + P+FA EYQ+GADSVILSTR Sbjct: 175 LNQKISRPFYFNYFNGWEDILKMLFPVMAMLITLVTSICVAPIFALEYQTGADSVILSTR 234 Query: 239 YGKSKLIQAKISAAFLFALLV-FTVNIVLAVGIQLYAFGTGGWNLPLQILNVTVPYNLSM 297 YGK+KLI AKI A L + FTV I+ +G+ L+ +GT GWN Q++++ Y S+ Sbjct: 235 YGKNKLIIAKILAVLLCVTFIYFTVVIIFTLGM-LWCYGTYGWNASFQVISIKSVYPYSV 293 Query: 298 LEAAVVCVSILYLVLLGMVAVTLLLSAKMKTAFXXXXXXXXXXXXXXXXGLSGTNGILNH 357 LE ++ V+I YLV+L + +LLS+K+KT N LN Sbjct: 294 LEVYLLGVAIGYLVILAFSSFIMLLSSKIKTP-AIMVISGLLIFVPILVRRDKENLALNR 352 Query: 358 ILMLLPYMAVQPVFPADYTSYFSYPLPGLTLSIITVRIVLYVVICSVCLPFVGRVFKRHQ 417 ++ L P AV +++SY +Y + L + +L V+ + +P FK H+ Sbjct: 353 VMNLFPGNAVNAF--VNFSSYDTYVFKNIVLLAPQLITILSVIGICIFVPIAFYNFKNHE 410 Query: 418 V 418 V Sbjct: 411 V 411 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,116,163,314 Number of extensions: 78323835 Number of successful extensions: 199270 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 199216 Number of HSP's successfully gapped: 49 Length of query: 419 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 281 Effective length of database: 1,760,596,133 Effective search space: 494727513373 Effective search space used: 494727513373 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)