BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0003.p (418 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I4D7.1 B8I4D7_CLOCE Putative uncharacterized protein 249 3e-64 B2V221.1 B2V221_CLOBA Putative uncharacterized protein 221 7e-56 C0GJJ7.1 C0GJJ7_9FIRM Putative uncharacterized protein 217 2e-54 A8MJQ7.1 A8MJQ7_ALKOO Putative uncharacterized protein 214 1e-53 B2TLU0.1 B2TLU0_CLOBB Putative FliB family protein 213 2e-53 >B8I4D7.1 B8I4D7_CLOCE Putative uncharacterized protein Length = 414 Score = 249 bits (636), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 135/411 (32%), Positives = 234/411 (56%), Gaps = 13/411 (3%) Query: 19 QKEYLTPQYIRQFQCTGAVCEDTCCSLWEITIDRSTYEKYKQIPDASLHEEINKNLVRNK 78 +K +L PQY R+F+C G+ CED+CC W+++ID+ TY+KY+ + + L ++K + R + Sbjct: 5 KKVFLAPQYFRKFKCIGSECEDSCCKGWKVSIDKETYKKYRCVQNVELRPLLDKYVTRER 64 Query: 79 KS---IESYASFKMNQKTGNCSMLCDG-LCTIQAKVGETFLSQTCSTYPRILNEADE-IE 133 K+ + +YA KM + G C L D LC IQ + GE +LS+ C+TYPR D IE Sbjct: 65 KNGGDLVNYAKIKMLDEEGICPFLSDKKLCRIQLEYGEGYLSKVCTTYPRNAIRVDNTIE 124 Query: 134 VSALLSCPEIARLVLLSEDAMSFTRTNELHTPNLRIS-----QLGRATNKKKQCIQNLRD 188 + LSCPE A+L LL + M F ++ P+L S Q + N+ ++ +LR Sbjct: 125 MVTSLSCPEAAKLALLDPEIMEFDEYEDIPNPSLMGSCAFNTQDKQFVNRPQRYFWDLRI 184 Query: 189 ITLEVLSDRQFSLQHRLIIIGVLLDNTDELLRSGAYDHVLSFVDEFRGELVTNEELRDYD 248 T+++L +R + + RLII+G+ ++ ++ + + + + + + D Sbjct: 185 FTIDILQNRNYFMWERLIILGLFIEKLNKSVDENKINDIPDIIQSYNKMIEEGTFKGALD 244 Query: 249 VFPSNDQIQFQVLNNTLMAHLSEFLWNARYNECKNEYLKGIQ-SNGSSLNESLHTYRSVR 307 P+ ++Q Q++ + + ++ + N Y EC +LKGI + ++ E ++ Y+ Sbjct: 245 SIPAECKVQLQLIKDIIDIRVNSGVLNKIYMECYEHFLKGINYTEEATKEEIINNYQKAF 304 Query: 308 EEIYEPYLREKEFIFENYAINHVYQNFMTDIYAGGSLFDFFVKLVADYSFIRLHLIGMAA 367 EE Y+PY+ E+I ENY +N+V+ ++ S+FD ++ LV Y+ I++HLIGMA Sbjct: 305 EEYYKPYMDSHEYILENYLVNYVFSRLFP--FSSESIFDAYMLLVLHYAIIKVHLIGMAG 362 Query: 368 YRQELNDDMVVKLIQSYTKNYKFSNKFLNAILKDIKDQGYYTLGGMSLLIK 418 Y + L DDMV+KLI ++TK + FL+ I K +K++ + TL M+++IK Sbjct: 363 YNKGLEDDMVIKLISTFTKIVDHNTVFLSTITKQLKEKRFNTLAYMTIMIK 413 >B2V221.1 B2V221_CLOBA Putative uncharacterized protein Length = 405 Score = 221 bits (564), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 129/409 (31%), Positives = 225/409 (55%), Gaps = 24/409 (5%) Query: 23 LTPQYIRQFQCTGAVCEDTCCSLWEITIDRSTYEKYKQIPDASLHEEINKNLVRNKKS-- 80 L P+Y++ F CT C+D CC W++T+D++TY+K + + S+ E++K ++RNK S Sbjct: 7 LIPEYMKNFHCTSVDCKDDCCYGWKVTVDKATYKKCRNVQKTSMRNELSKYILRNKDSKI 66 Query: 81 IESYASFKMNQKTGNCSMLC-DGLCTIQAKVGETFLSQTCSTYPRILNEADEI-EVSALL 138 YA + Q C C + LC I +GE +LS TC YPR N+ D I EVS + Sbjct: 67 FNDYAYIPIEQDKS-CPFQCEEKLCKIHRDLGEEYLSLTCKNYPRFYNKVDSIFEVSLDM 125 Query: 139 SCPEIARLVLLSEDAMSFTRTNELHTPNLRISQLGRATNKKKQCIQN----LRDITLEVL 194 SCPE+ +LVL +++ ++F E T + +G A + K I N +R + +L Sbjct: 126 SCPEVVKLVLFNKELLAFE---EAETDKDLNNSIGIAMDTKSISIYNYFWDIRVGVITIL 182 Query: 195 SDRQFSLQHRLIIIGVLLDNTDELLRSGAYDHVLSFVDEFRGELVTNEELRDYDVFPSND 254 +R+ S++ RLI++G++++ +E ++G + + ++ + ++ E D P+N Sbjct: 183 QNRKLSIEQRLILVGLMINKIEESKKNGNLESIPDHIEYYLNKIDIYELRELIDSLPNNL 242 Query: 255 QIQFQVLNNTLMAHLSEFLWNARYNECKNEYLKGIQ-----SNGSSLNESLHTYRSVREE 309 ++Q + L+ + F +N E E++K + + +NE + Y+ R+E Sbjct: 243 EVQMK-----LLEQIISFSYNGTLRENYAEHIKKFKQGLKIQDEIQINECIKAYKKSRKE 297 Query: 310 IYEPYLREKEFIFENYAINHVYQNFMTDIYAGGSLFDFFVKLVADYSFIRLHLIGMAAYR 369 YEPY++E+E+IFENY +N V+Q Y + + ++ LV +YS IRL L GMA+ Sbjct: 298 YYEPYIKEREYIFENYLVNDVFQRMFP--YRDDNFLNEYMLLVMNYSIIRLVLTGMASCD 355 Query: 370 QELNDDMVVKLIQSYTKNYKFSNKFLNAILKDIKDQGYYTLGGMSLLIK 418 ++L D MVV LI S+++ +N F+ + + + + + TL M +LIK Sbjct: 356 EQLTDTMVVDLIYSFSRAVLHNNAFIKSFIDALNENNFNTLSHMIILIK 404 >C0GJJ7.1 C0GJJ7_9FIRM Putative uncharacterized protein Length = 413 Score = 217 bits (552), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 134/406 (33%), Positives = 211/406 (51%), Gaps = 12/406 (2%) Query: 23 LTPQYIRQFQCTGAVCEDTCCSLWEITIDRSTYEKYKQIPDASLHEEINKNLVR--NKKS 80 L P+Y++ F C G+ CED+CC W + +D+ TY YK++ L I K + R N KS Sbjct: 9 LQPEYLKNFHCIGSDCEDSCCIGWRVDLDKKTYLAYKKLNQPELRLTIEKMVNRKHNDKS 68 Query: 81 IESYASFKMNQKTGNCSML-CDGLCTIQAKVGETFLSQTCSTYPRILNEAD-EIEVSALL 138 Y KM+ + G C L + LC I GE +LS TC+ YPR + + D E SA Sbjct: 69 NACYGKIKMD-RNGKCPFLNKENLCKIYIFAGEEYLSNTCTFYPRDVKKIDGAFERSATP 127 Query: 139 SCPEIARLVLLSEDAMSFTRTNELHTPNLRI-----SQLGRATNKKKQCIQNLRDITLEV 193 SCPEI RL +LS + +SF + E ++I +Q NK ++ +R +L + Sbjct: 128 SCPEIVRLGILSLEGISFEQVEENAEETIQIHGTFDTQGHLFLNKPQRFFWEIRMFSLCL 187 Query: 194 LSDRQFSLQHRLIIIGVLLDNTDELLRSGAYDHVLSFVDEFRGELVTNEELRDYDVFPSN 253 L +R +SL RL+I+G++ + + G + ++ + + P Sbjct: 188 LQNRDYSLAERLVILGIVYKKIEVIQLEGRVRDIPLMLETMNSMIEAGNFKSELAKIPIE 247 Query: 254 DQIQFQVLNNTLMAHLSEFLWNARYNECKNEYLKGIQ-SNGSSLNESLHTYRSVREEIYE 312 ++Q QV S + + RY EC E +GI + + Y +E Sbjct: 248 SEVQMQVAKTIANHKFSLGISSKRYMECLKETFQGIGFVETDDIEQVKDKYDRNYQEFLR 307 Query: 313 PYLREKEFIFENYAINHVYQNFMTDIYAGGSLFDFFVKLVADYSFIRLHLIGMAAYRQEL 372 PYL+EKE I ENY +N ++ M + S++D ++ L Y+ I+ H+IGMA Y +EL Sbjct: 308 PYLKEKEHILENYLVNEFFKEMMP-FGSYQSIWDSYIFLCLLYAMIKFHMIGMAGYHKEL 366 Query: 373 NDDMVVKLIQSYTKNYKFSNKFLNAILKDIKDQGYYTLGGMSLLIK 418 ND++V+K+IQS+TK +N+++ I+K +KD GY TL MS+L+K Sbjct: 367 NDELVIKIIQSFTKVVLHNNQYIQQIIKLLKDSGYDTLAYMSILVK 412 >A8MJQ7.1 A8MJQ7_ALKOO Putative uncharacterized protein Length = 413 Score = 214 bits (546), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 133/408 (32%), Positives = 230/408 (56%), Gaps = 20/408 (4%) Query: 23 LTPQYIRQFQCTGAVCEDTCCSLWEITIDRSTYEKYKQIPDASLHEEINKNLVR---NKK 79 L P+Y++QF+C G+ CED+CC W +++D+ Y+ Y++ + L + I K++VR N K Sbjct: 9 LMPEYMKQFRCIGSKCEDSCCIGWTVSLDKKRYQNYRKNNNMEL-KPIFKSMVRRSHNNK 67 Query: 80 SIESYASFKMNQKTGNCSMLCDG-LCTIQAKVGETFLSQTCSTYPRILNEAD-EIEVSAL 137 + E Y M++ G C L + LC IQ +GE +LS C++YPR + D ++E SA Sbjct: 68 TDEFYGRIVMDE-LGRCPFLDESKLCKIQDILGEEYLSNICASYPRFTSRIDGKLERSAT 126 Query: 138 LSCPEIARLVLLSEDAMSFTRTNELHTPNLRI-------SQLGRATNKKKQCIQNLRDIT 190 SCPEIARL LL+ + + F + E +LRI ++ NK ++ ++R Sbjct: 127 TSCPEIARLALLNPNGICFEQIQE--DEDLRIMINSSFDTEGYSLYNKPEKYFWDIRLFC 184 Query: 191 LEVLSDRQFSLQHRLIIIGVLLDNTDELLRSGAYDHVLSFVDEFRGELVTNEELRD-YDV 249 + +L DR +SL R++++G++ +N E L H + V E ++++ R+ D Sbjct: 185 ISLLQDRDYSLSERVMLLGIV-NNRIEGLYKMQKTHDIPKVLESLAKMISAGNFREELDK 243 Query: 250 FPSNDQIQFQVLNNTLMAHLSEFLWNARYNECKNEYLKGIQ-SNGSSLNESLHTYRSVRE 308 P+ +IQ ++L + L N RY EC NE L G++ + + ++ Y + Sbjct: 244 VPTQVEIQLKILETMINERLLIGTLNERYIECLNETLLGLENTEDEKIETTIKKYEEAYD 303 Query: 309 EIYEPYLREKEFIFENYAINHVYQNFMTDIYAGGSLFDFFVKLVADYSFIRLHLIGMAAY 368 + YL+EKE+I EN+ +N ++ +M S++D ++ + Y I+LHL+GMA Y Sbjct: 304 SYLKDYLKEKEYILENFLVNEYFR-WMMPFGGFTSIWDSYIFICVVYGMIKLHLVGMAEY 362 Query: 369 RQELNDDMVVKLIQSYTKNYKFSNKFLNAILKDIKDQGYYTLGGMSLL 416 +ELND++ +KLIQS++K +N+++ +I+ IK+ G TL M++L Sbjct: 363 HKELNDELTLKLIQSFSKVILHNNQYIQSIINLIKENGLDTLAYMTIL 410 >B2TLU0.1 B2TLU0_CLOBB Putative FliB family protein Length = 405 Score = 213 bits (543), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 124/405 (30%), Positives = 225/405 (55%), Gaps = 16/405 (3%) Query: 23 LTPQYIRQFQCTGAVCEDTCCSLWEITIDRSTYEKYKQIPDASLHEEINKNLVRNKKS-- 80 L P+Y++ F CT C+D CC W++T+D++TY+K + + S+ E++K ++RNK S Sbjct: 7 LVPEYMKNFHCTSLGCKDDCCYGWKVTVDKATYKKCRNVQKTSVRNELSKYILRNKDSKI 66 Query: 81 IESYASFKMNQKTGNCSMLC-DGLCTIQAKVGETFLSQTCSTYPRILNEADEI-EVSALL 138 YA + Q C C + LC I +GE +LS TC YPR N+ D I EVS + Sbjct: 67 FNDYAYISIEQDKS-CPFQCEEKLCKIHRDLGEEYLSLTCKNYPRFYNKVDSIFEVSLDM 125 Query: 139 SCPEIARLVLLSEDAMSFTRTNELHTPNLRISQLGRATNKKKQCIQN----LRDITLEVL 194 SCPE+ +LVL +++ ++F N + LG + K I N +R + +L Sbjct: 126 SCPEVVKLVLFNKELLAFEEVEADKDLN---NSLGVVMDTKSISICNYFWDIRVGVITIL 182 Query: 195 SDRQFSLQHRLIIIGVLLDNTDELLRSGAYDHVLSFVDEFRGELVTNEELRDYDVFPSND 254 +R+ S++ RLI++G++++ +E +S + + +++ + ++ +E D P+N Sbjct: 183 QNRKLSIEQRLILVGLMINKIEESKKSSNLESIPDYIEYYVNKIDIDELRELIDSLPNNL 242 Query: 255 QIQFQVLNNTLMAHLSEFLWNARYNECKNEYLKGIQ-SNGSSLNESLHTYRSVREEIYEP 313 ++Q ++L + + L Y E ++ KG++ + +++ + Y+ +E YEP Sbjct: 243 EVQMKLLEQIIAFSYNSTL-RENYVEHIEKFKKGLKIQDEIQIDKCIKAYKESVKEYYEP 301 Query: 314 YLREKEFIFENYAINHVYQNFMTDIYAGGSLFDFFVKLVADYSFIRLHLIGMAAYRQELN 373 Y++E+++IFENY +N V+Q Y + F+ ++ LV +YS IRL L G+A+ ++L Sbjct: 302 YIKERKYIFENYLVNDVFQRIFP--YRDDNFFNEYMLLVINYSIIRLVLTGIASCDKQLT 359 Query: 374 DDMVVKLIQSYTKNYKFSNKFLNAILKDIKDQGYYTLGGMSLLIK 418 D MVV LI S+++ +N F+ + + + + + TL M +LIK Sbjct: 360 DAMVVDLIYSFSRAVLHNNAFIKSFIDALNENNFNTLSHMIILIK 404 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,367,712,420 Number of extensions: 94577304 Number of successful extensions: 243984 Number of sequences better than 1.0e-05: 157 Number of HSP's gapped: 243603 Number of HSP's successfully gapped: 157 Length of query: 418 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 280 Effective length of database: 1,760,596,133 Effective search space: 492966917240 Effective search space used: 492966917240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)