BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0007.p (463 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3J102.1 C3J102_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 586 e-165 B7Y9V3.1 B7Y9V3_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 586 e-165 Q5L011.1 Q5L011_GEOKA Malic enzyme EC=1.1.1.38 584 e-165 B4BKJ0.1 B4BKJ0_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 584 e-165 P16468.1 MAOX_BACST NAD-dependent malic enzyme EC=1.1.1.38 582 e-164 >C3J102.1 C3J102_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 478 Score = 586 bits (1510), Expect = e-165, Method: Compositional matrix adjust. Identities = 290/457 (63%), Positives = 344/457 (75%), Gaps = 1/457 (0%) Query: 1 MIIRLEIRKSVATFGDVASRLAAVGGDIVAIDVIRAGKDVTTRDITVNVQDAAN-EEIIT 59 + IRL+ K + +FGD+A+ + GGDIV IDVI + K T RDITV+ D + II Sbjct: 11 ITIRLQFEKDIVSFGDIAAAIGKAGGDIVGIDVISSSKVHTVRDITVSALDTKQCDLIIE 70 Query: 60 VLKDMPGIKVINISDRTFLAHIGGKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPG 119 LK + G+K+IN+SDRTFL HIGGKIE KIP+K R+DLS VYTPGVARVC AIAEDP Sbjct: 71 ALKKIQGVKIINVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPR 130 Query: 120 RAYSLTMKRNTVAVVTDGTAVLGLGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKD 179 +AYSLT+KRNTVAVV+DGTAVLGLGDIGP AAMPVMEGKAMLFK+ A +DAFP+CL TKD Sbjct: 131 KAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLNTKD 190 Query: 180 PEEIIRVVKAVTAGLGGINLEDISSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXX 239 EEII++VKA+ GGINLEDIS+PRCFEIE+RL ELDIPVFHDDQHGT Sbjct: 191 TEEIIQIVKAIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLL 250 Query: 240 XXXXXXGKRLEDCRXXXXXXXXXXXSICRLLLAAGARKVYAVDRAGILHKGQTYDNAEWS 299 K+LED + + ++LLAAG R + VDR G +H+ +TY+N W Sbjct: 251 NALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQ 310 Query: 300 WLAEATNPEGLTGGLTEAMQGADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEP 359 A+ TNP+ L G L++ + GADVFIGVS+ GIL + +K MA D IVFAMANP PEI+P Sbjct: 311 EYAQITNPDNLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDP 370 Query: 360 ELAESYARVVATGRSDYPNQINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHP 419 ELAE Y RV+ATGRSDYPNQINNVLCFPGIFRGALDCRA+ +N EMKLAAA+AIASVV Sbjct: 371 ELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTE 430 Query: 420 DELNEQYIIPSIFNEKVVEGVREAVIRAAIATGMARR 456 DELNE YIIPS+FN KVVE VR+AVI AA TG+AR+ Sbjct: 431 DELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 467 >B7Y9V3.1 B7Y9V3_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 478 Score = 586 bits (1510), Expect = e-165, Method: Compositional matrix adjust. Identities = 290/457 (63%), Positives = 344/457 (75%), Gaps = 1/457 (0%) Query: 1 MIIRLEIRKSVATFGDVASRLAAVGGDIVAIDVIRAGKDVTTRDITVNVQDAAN-EEIIT 59 + IRL+ K + +FGD+A+ + GGDIV IDVI + K T RDITV+ D + II Sbjct: 11 ITIRLQFEKDIVSFGDIAAAIGKAGGDIVGIDVISSSKVHTVRDITVSALDTKQCDLIIE 70 Query: 60 VLKDMPGIKVINISDRTFLAHIGGKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPG 119 LK + G+K+IN+SDRTFL HIGGKIE KIP+K R+DLS VYTPGVARVC AIAEDP Sbjct: 71 ALKKIQGVKIINVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPR 130 Query: 120 RAYSLTMKRNTVAVVTDGTAVLGLGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKD 179 +AYSLT+KRNTVAVV+DGTAVLGLGDIGP AAMPVMEGKAMLFK+ A +DAFP+CL TKD Sbjct: 131 KAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLNTKD 190 Query: 180 PEEIIRVVKAVTAGLGGINLEDISSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXX 239 EEII++VKA+ GGINLEDIS+PRCFEIE+RL ELDIPVFHDDQHGT Sbjct: 191 TEEIIQIVKAIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLL 250 Query: 240 XXXXXXGKRLEDCRXXXXXXXXXXXSICRLLLAAGARKVYAVDRAGILHKGQTYDNAEWS 299 K+LED + + ++LLAAG R + VDR G +H+ +TY+N W Sbjct: 251 NALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQ 310 Query: 300 WLAEATNPEGLTGGLTEAMQGADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEP 359 A+ TNP+ L G L++ + GADVFIGVS+ GIL + +K MA D IVFAMANP PEI+P Sbjct: 311 EYAQITNPDNLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDP 370 Query: 360 ELAESYARVVATGRSDYPNQINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHP 419 ELAE Y RV+ATGRSDYPNQINNVLCFPGIFRGALDCRA+ +N EMKLAAA+AIASVV Sbjct: 371 ELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTE 430 Query: 420 DELNEQYIIPSIFNEKVVEGVREAVIRAAIATGMARR 456 DELNE YIIPS+FN KVVE VR+AVI AA TG+AR+ Sbjct: 431 DELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 467 >Q5L011.1 Q5L011_GEOKA Malic enzyme EC=1.1.1.38 Length = 478 Score = 584 bits (1506), Expect = e-165, Method: Compositional matrix adjust. Identities = 289/457 (63%), Positives = 343/457 (75%), Gaps = 1/457 (0%) Query: 1 MIIRLEIRKSVATFGDVASRLAAVGGDIVAIDVIRAGKDVTTRDITVNVQDAAN-EEIIT 59 + IRL+ K + +F D+A+ + GGDIV IDVI + K T RDITV+ D + II Sbjct: 11 ITIRLQFEKDIVSFSDIAAAIGKAGGDIVGIDVISSSKVHTVRDITVSALDTKQCDLIIE 70 Query: 60 VLKDMPGIKVINISDRTFLAHIGGKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPG 119 LK + G+K+IN+SDRTFL HIGGKIE KIP+K R+DLS VYTPGVARVC AIAEDP Sbjct: 71 ALKKIQGVKIINVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPR 130 Query: 120 RAYSLTMKRNTVAVVTDGTAVLGLGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKD 179 +AYSLT+KRNTVAVV+DGTAVLGLGDIGP AAMPVMEGKAMLFK+ A +DAFP+CL TKD Sbjct: 131 KAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLNTKD 190 Query: 180 PEEIIRVVKAVTAGLGGINLEDISSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXX 239 EEII++VKA+ GGINLEDIS+PRCFEIE+RL ELDIPVFHDDQHGT Sbjct: 191 TEEIIQIVKAIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLL 250 Query: 240 XXXXXXGKRLEDCRXXXXXXXXXXXSICRLLLAAGARKVYAVDRAGILHKGQTYDNAEWS 299 K+LED + + ++LLAAG R + VDR G +H+ +TY+N W Sbjct: 251 NALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQ 310 Query: 300 WLAEATNPEGLTGGLTEAMQGADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEP 359 A+ TNP+ L G L++ + GADVFIGVS+ GIL + +K MA D IVFAMANP PEI+P Sbjct: 311 EYAQITNPDNLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDP 370 Query: 360 ELAESYARVVATGRSDYPNQINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHP 419 ELAE Y RV+ATGRSDYPNQINNVLCFPGIFRGALDCRA+ +N EMKLAAA+AIASVV Sbjct: 371 ELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTE 430 Query: 420 DELNEQYIIPSIFNEKVVEGVREAVIRAAIATGMARR 456 DELNE YIIPS+FN KVVE VR+AVI AA TG+AR+ Sbjct: 431 DELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 467 >B4BKJ0.1 B4BKJ0_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 470 Score = 584 bits (1505), Expect = e-165, Method: Compositional matrix adjust. Identities = 290/457 (63%), Positives = 342/457 (74%), Gaps = 1/457 (0%) Query: 1 MIIRLEIRKSVATFGDVASRLAAVGGDIVAIDVIRAGKDVTTRDITVNVQDAAN-EEIIT 59 + IRL+ K + +F D+A+ + GGDIV IDVI + K T RDITV+ D + II Sbjct: 3 ITIRLQFEKDIVSFSDIAAAIGKAGGDIVGIDVISSSKVHTVRDITVSALDTKQCDLIIE 62 Query: 60 VLKDMPGIKVINISDRTFLAHIGGKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPG 119 LK + G+K++N+SDRTFL HIGGKIE KIP+K R+DLS VYTPGVARVC AIAEDP Sbjct: 63 ALKKIRGVKIVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPR 122 Query: 120 RAYSLTMKRNTVAVVTDGTAVLGLGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKD 179 +AYSLT+KRNTVAVV+DGTAVLGLGDIGP AAMPVMEGKAMLFKQ A +DAFP+CL TKD Sbjct: 123 KAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKQFAGVDAFPVCLDTKD 182 Query: 180 PEEIIRVVKAVTAGLGGINLEDISSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXX 239 EEII++VKA+ GGINLEDIS+PRCFEIE RL ELDIPVFHDDQHGT Sbjct: 183 TEEIIQIVKAIAPAFGGINLEDISAPRCFEIEARLKEELDIPVFHDDQHGTAVVLLAGLL 242 Query: 240 XXXXXXGKRLEDCRXXXXXXXXXXXSICRLLLAAGARKVYAVDRAGILHKGQTYDNAEWS 299 K+LED + + ++LLAAG R + VDR G +H+ +TY+N W Sbjct: 243 NALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRYGAIHRDETYENPYWQ 302 Query: 300 WLAEATNPEGLTGGLTEAMQGADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEP 359 A+ TNP+ L G L++ + GADVFIGVS+ GIL + +K MA D IVFAMANP PEI+P Sbjct: 303 EYAQITNPDNLKGSLSDVIVGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPVPEIDP 362 Query: 360 ELAESYARVVATGRSDYPNQINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHP 419 ELAE Y RV+ATGRSDYPNQINNVLCFPGIFRGALDCRAK +N EMKLAAA+AIASVV Sbjct: 363 ELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAKEINEEMKLAAAKAIASVVTE 422 Query: 420 DELNEQYIIPSIFNEKVVEGVREAVIRAAIATGMARR 456 DELNE YIIPS+FN KVVE VR+AVI AA TG+AR+ Sbjct: 423 DELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 459 >P16468.1 MAOX_BACST NAD-dependent malic enzyme EC=1.1.1.38 Length = 478 Score = 582 bits (1500), Expect = e-164, Method: Compositional matrix adjust. Identities = 287/457 (62%), Positives = 343/457 (75%), Gaps = 1/457 (0%) Query: 1 MIIRLEIRKSVATFGDVASRLAAVGGDIVAIDVIRAGKDVTTRDITVNVQDAAN-EEIIT 59 + IRL+ K + +F D+A+ + GGDIV IDVI + K T RDITV+ D + II Sbjct: 11 ITIRLQFEKDIVSFSDIAAAIGKAGGDIVGIDVISSSKVHTVRDITVSALDTKQCDLIIE 70 Query: 60 VLKDMPGIKVINISDRTFLAHIGGKIEITPKIPIKNREDLSLVYTPGVARVCMAIAEDPG 119 LK + G+K++N+SDRTFL HIGGKIE KIP+K R+DLS VYTPGVARVC AIAEDP Sbjct: 71 ALKKIRGVKIVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPR 130 Query: 120 RAYSLTMKRNTVAVVTDGTAVLGLGDIGPEAAMPVMEGKAMLFKQLADIDAFPLCLGTKD 179 +AYSLT+KRNTVAVV+DGTAVLGLGDIGP AAMPVMEGKAMLFK+ A +DAFP+CL TKD Sbjct: 131 KAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLDTKD 190 Query: 180 PEEIIRVVKAVTAGLGGINLEDISSPRCFEIERRLSAELDIPVFHDDQHGTXXXXXXXXX 239 EEII++VKA+ GGINLEDIS+PRCFEIE+RL ELDIPVFHDDQHGT Sbjct: 191 TEEIIQIVKAIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLL 250 Query: 240 XXXXXXGKRLEDCRXXXXXXXXXXXSICRLLLAAGARKVYAVDRAGILHKGQTYDNAEWS 299 K+LED + + ++LLAAG R + VDR G +H+ +TY+N W Sbjct: 251 NALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYWQ 310 Query: 300 WLAEATNPEGLTGGLTEAMQGADVFIGVSSGGILSEDHLKSMAEDNIVFAMANPTPEIEP 359 A+ TNP+ L G L++ + GADVFIGVS+ GIL + +K MA D IVFAMANP PEI+P Sbjct: 311 EYAQLTNPDNLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEIDP 370 Query: 360 ELAESYARVVATGRSDYPNQINNVLCFPGIFRGALDCRAKVVNLEMKLAAAQAIASVVHP 419 ELAE Y RV+ATGRSDYPNQINNVLCFPGIFRGALDCRA+ +N EMKLAAA+AIASVV Sbjct: 371 ELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTE 430 Query: 420 DELNEQYIIPSIFNEKVVEGVREAVIRAAIATGMARR 456 DELNE YIIPS+FN KVVE VR+AV+ AA TG+AR+ Sbjct: 431 DELNETYIIPSVFNSKVVERVRQAVVEAAYRTGVARK 467 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,485,340,760 Number of extensions: 97439049 Number of successful extensions: 283062 Number of sequences better than 1.0e-05: 1912 Number of HSP's gapped: 278805 Number of HSP's successfully gapped: 1920 Length of query: 463 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 324 Effective length of database: 1,751,175,118 Effective search space: 567380738232 Effective search space used: 567380738232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)