BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0006.p (181 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P45900.1 YQAC_BACSU Uncharacterized protein yqaC 206 1e-51 A7Z6Z6.1 A7Z6Z6_BACA2 YqaC 204 2e-51 A5FEV5.1 A5FEV5_FLAJ1 Adenylate kinase and related kinase-like p... 134 3e-30 A8I5U5.1 A8I5U5_AZOC5 Adenylate kinase 129 9e-29 A8TMX4.1 A8TMX4_9PROT Putative uncharacterized protein 129 1e-28 >P45900.1 YQAC_BACSU Uncharacterized protein yqaC Length = 178 Score = 206 bits (523), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 94/172 (54%), Positives = 121/172 (70%) Query: 1 MYSRIHIMGASGAGTSTLGRALAGHLPHVHLDSDDYFWEHKYTQQTELTERLRTIQRDLD 60 M IHI+GASG GTSTLG AL+ LPH HLD+D+Y+W K+T++ E+ ER + +++DL Sbjct: 1 MKKNIHILGASGVGTSTLGAALSKRLPHTHLDTDNYYWLDKFTKKREIPERRKLLEKDLT 60 Query: 61 QQEPYILSGAVCGWGDGLRPRFDLVVFLWIPPXXXXXXXXXXXXXXYGADSLPGGSMYQE 120 E +ILSGAVCGWGD L+ FDLVVFLWIP YG + L GGS Y++ Sbjct: 61 INEKWILSGAVCGWGDNLKSYFDLVVFLWIPQDIRLERLRHREFQRYGNEVLAGGSKYEQ 120 Query: 121 VQTFMEWAALYDTAGPEVRSRVLHEEWMSHLQCPVLRLEEDMTVAARVEAVL 172 +TF+EWA+LYD AG EVRSR LHE WM+ L CPVL++E+D +V RV+ VL Sbjct: 121 SKTFLEWASLYDNAGMEVRSRALHEHWMADLSCPVLKIEDDCSVNERVDRVL 172 >A7Z6Z6.1 A7Z6Z6_BACA2 YqaC Length = 177 Score = 204 bits (520), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 92/170 (54%), Positives = 122/170 (71%) Query: 3 SRIHIMGASGAGTSTLGRALAGHLPHVHLDSDDYFWEHKYTQQTELTERLRTIQRDLDQQ 62 + IHI+GASG GTSTLG AL+ HLPH+HLD+DDY+W K+T++ E+ ER + +++DL Sbjct: 2 NNIHILGASGVGTSTLGVALSKHLPHMHLDTDDYYWTDKFTKKREIPERRKLLEKDLTIN 61 Query: 63 EPYILSGAVCGWGDGLRPRFDLVVFLWIPPXXXXXXXXXXXXXXYGADSLPGGSMYQEVQ 122 E +ILSGAVCGWGD L+ FDLV+FLWIP YG + L GGS Y++ + Sbjct: 62 EKWILSGAVCGWGDDLKSYFDLVIFLWIPQDIRLERLRHREFQRYGNEVLAGGSKYEQSK 121 Query: 123 TFMEWAALYDTAGPEVRSRVLHEEWMSHLQCPVLRLEEDMTVAARVEAVL 172 TF+EWA+LYD AG EVRS+ LHE WM+ L CP+L++E D +V RV+ VL Sbjct: 122 TFLEWASLYDNAGMEVRSKALHENWMADLSCPLLKIEGDYSVNERVDRVL 171 >A5FEV5.1 A5FEV5_FLAJ1 Adenylate kinase and related kinase-like protein Length = 183 Score = 134 bits (338), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 67/179 (37%), Positives = 97/179 (54%), Gaps = 4/179 (2%) Query: 4 RIHIMGASGAGTSTLGRALAGHLPHVHLDSDDYFWEHK---YTQQTELTERLRTIQRDLD 60 +IHI GASG+G +T G ALA L + + DSDDYFWE +T + ER I+ DL+ Sbjct: 2 KIHIFGASGSGVTTTGNALAEKLAYRYFDSDDYFWEKSDVPFTLRRNPEERNSKIKSDLE 61 Query: 61 QQEPYILSGAVCGWGDGLRPRFDLVVFLWIPPXXXXXXXXXXXXXXYGADSLPGGSMYQE 120 E +IL G++ WG+ + P FDLVV+LWIPP YG ++ Sbjct: 62 NSENWILGGSIFQWGENVFPDFDLVVYLWIPPQIRMERLKKREFERYGNVIYTNPDRIKQ 121 Query: 121 VQTFMEWAALYD-TAGPEVRSRVLHEEWMSHLQCPVLRLEEDMTVAARVEAVLREYAAK 178 + FMEWAA YD G R+ HE W++ + PVL++ D+T+ R++ ++ K Sbjct: 122 FEDFMEWAADYDNNTGIASRNFQAHENWLNSIDFPVLKISGDLTLQQRIDLIIENINKK 180 >A8I5U5.1 A8I5U5_AZOC5 Adenylate kinase Length = 183 Score = 129 bits (325), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 73/171 (42%), Positives = 96/171 (56%), Gaps = 3/171 (1%) Query: 5 IHIMGASGAGTSTLGRALAGHLPHVHLDSDDYFW---EHKYTQQTELTERLRTIQRDLDQ 61 IHI GASG+GTSTLG A+A L HLD+D +FW E +T E++ RL +Q++ Sbjct: 4 IHITGASGSGTSTLGAAVAEVLGIRHLDTDRFFWMPTEPPFTTPREVSARLALLQQEALP 63 Query: 62 QEPYILSGAVCGWGDGLRPRFDLVVFLWIPPXXXXXXXXXXXXXXYGADSLPGGSMYQEV 121 ++LSG+ W L P +DL+VFL + P YG PGG M + Sbjct: 64 DVGWVLSGSALKWATPLEPLYDLIVFLHLDPAVRMERIRLREEQRYGERIRPGGDMAEVS 123 Query: 122 QTFMEWAALYDTAGPEVRSRVLHEEWMSHLQCPVLRLEEDMTVAARVEAVL 172 + F+EWA YDTAGPE RSR HE+W+ PVLRL+ V A + AVL Sbjct: 124 RAFLEWAESYDTAGPERRSRAAHEDWLQRQSAPVLRLDSVEPVEALLAAVL 174 >A8TMX4.1 A8TMX4_9PROT Putative uncharacterized protein Length = 213 Score = 129 bits (324), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 70/173 (40%), Positives = 100/173 (57%), Gaps = 4/173 (2%) Query: 4 RIHIMGASGAGTSTLGRALAGHLPHVHLDSDDYFW---EHKYTQQTELTERLRTIQRDLD 60 RIHI+GASG+GT+TLGR LAG + H D+DDYFW + +T++ +RLR + Sbjct: 25 RIHILGASGSGTTTLGRVLAGEMAVPHHDTDDYFWLPTDPPFTEKRPEADRLRLMTELFT 84 Query: 61 QQEPYILSGAVCGWGDGLRPRFDLVVFLWIPPXXXXXXXXXXXXXXYGADSL-PGGSMYQ 119 + ++LSG++ GWGD L P FDLVVFL P YG ++ GG+ ++ Sbjct: 85 PRAAWVLSGSLVGWGDPLIPLFDLVVFLQTPAATRLARLRDREVLRYGQAAVGSGGTHHE 144 Query: 120 EVQTFMEWAALYDTAGPEVRSRVLHEEWMSHLQCPVLRLEEDMTVAARVEAVL 172 + F+ WAA YD G E RS HEEW++ L CP +RL+ + V ++L Sbjct: 145 ATEGFLNWAAGYDGGGLEGRSLRRHEEWLAGLPCPAVRLDGSRPIPELVASLL 197 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,034,398,986 Number of extensions: 37938263 Number of successful extensions: 165140 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 165133 Number of HSP's successfully gapped: 28 Length of query: 181 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 54 Effective length of database: 1,864,227,298 Effective search space: 100668274092 Effective search space used: 100668274092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)