BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0004.p (263 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGC2.1 B1DGC2_9BACL Putative uncharacterized protein 139 2e-31 A9GT92.1 A9GT92_SORC5 Putative membrane protein 130 1e-28 C1TFC4.1 C1TFC4_9FIRM ABC-type uncharacterized transport system,... 129 3e-28 A7NKA1.1 A7NKA1_ROSCS Putative uncharacterized protein 125 3e-27 A9KKD4.1 A9KKD4_CLOPH Putative uncharacterized protein 123 2e-26 >B1DGC2.1 B1DGC2_9BACL Putative uncharacterized protein Length = 260 Score = 139 bits (351), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 81/259 (31%), Positives = 140/259 (54%), Gaps = 3/259 (1%) Query: 7 IYFKYLRMHLLSGMEYK-GWWLMLIQVLVVVISDPIATVLLFSRFGNIGEWTVAHIILVY 65 +Y KY+ + S +Y+ +WL+ + + ++ +F++FG + WT L Y Sbjct: 3 LYLKYISILFKSQAQYRLSFWLLTVGQALTPLTVLAGLYFMFAQFGQVKGWTFYEAALCY 62 Query: 66 ALAVASFGLAESLCRGFDYFPWQMLRSGDFDRLLLRPRSLFVQVAASRFHLHRLVRPVTG 125 A+ S+ L+E+ RGFD F ++ SGDFDRLL+RPRS +QV S+F RL R + Sbjct: 63 AIIGLSYALSETFARGFDMFS-SLVGSGDFDRLLVRPRSTILQVLGSKFEFARLGRMLQS 121 Query: 126 ICAMGWALSELGVSLTPGKLLVLAMALAGGCVMYCGVFVLTSGLAFFTIKGLDWIYLLTN 185 + + W+LS+L + LT KL L + + G +++ G+F+ T+ L+F+TI+GL+ + T+ Sbjct: 122 LIVLLWSLSKLPIELTLPKLFTLMLMIVCGVLIFSGLFIATASLSFWTIQGLEVANVFTD 181 Query: 186 ASYQITRCPEPYMPRALKSMFSFVLPMLFISFYPAATVCG-WDYPQWXXXXXXXXXXXXX 244 ++ + P + + + F+FV+P+ +++ P V G D + Sbjct: 182 GGREMAKYPLSIYEKWVTNFFTFVIPLGCVNYLPLLYVLGRTDGNRLLYMLSPLAGVVFI 241 Query: 245 XXXXXVWRFGVRHYKSTGS 263 +W GVRHY+STGS Sbjct: 242 LPCLLLWHIGVRHYRSTGS 260 >A9GT92.1 A9GT92_SORC5 Putative membrane protein Length = 268 Score = 130 bits (327), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 89/264 (33%), Positives = 133/264 (50%), Gaps = 6/264 (2%) Query: 5 SKIYFKYLRMHLLSGMEYKGWWLM-LIQVLVVVISDPIATVLLFSRFGNIGEWTVAHIIL 63 +++Y + L + M+Y+ ++M + + V + + LF RFG + W A + L Sbjct: 6 TRLYLRLAAASLRAQMQYRASFVMWSVGQFITVGIELLGAWALFDRFGAVRGWRFAEVAL 65 Query: 64 VYALAVASFGLAESLCRGFDYFPWQMLRSGDFDRLLLRPRSLFVQVAASRFHLHRLVRPV 123 ++ L SF LAES RGFD F M++SGDFDRLLLRPRS QVA F R R Sbjct: 66 LFGLVNVSFALAESFGRGFDTFS-AMVKSGDFDRLLLRPRSTAFQVATGEFQFLRAGRLA 124 Query: 124 TGICAMGWALSELGVSLTPGKLLVLAMALAGGCVMYCGVFVLTSGLAFFTIKGLDWIYLL 183 G+ + WA S L V T KL +LA A+ GG ++ G+ VL + L F+T++ L+ + L Sbjct: 125 VGLVMLLWAGSALSVGWTAAKLALLAGAIVGGACVFYGLLVLQATLCFWTVESLEIMNAL 184 Query: 184 TNASYQITRCPEPYMPRALKSMFSFVLPMLFISFYPAATVCGW----DYPQWXXXXXXXX 239 T + + P + F+FV+P+ F+S+ PA + P+ Sbjct: 185 TYGGTEAAQYPLTLYRDWFRRFFTFVVPLAFVSYIPAGALLDRPTVPALPEAARWASPLV 244 Query: 240 XXXXXXXXXXVWRFGVRHYKSTGS 263 +WRFG R Y+STGS Sbjct: 245 GVAFLLVSLRIWRFGERRYQSTGS 268 >C1TFC4.1 C1TFC4_9FIRM ABC-type uncharacterized transport system, permease component Length = 258 Score = 129 bits (324), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 76/258 (29%), Positives = 133/258 (51%), Gaps = 3/258 (1%) Query: 7 IYFKYLRMHLLSGMEYK-GWWLMLIQVLVVVISDPIATVLLFSRFGNIGEWTVAHIILVY 65 +Y K+ MHL S M+YK ++L +V + + +F+RF + + ++L + Sbjct: 3 LYLKFFSMHLKSQMQYKVSFFLTAAGQFLVSFTSLLGVYFMFTRFNEVEGFIFQQVLLCF 62 Query: 66 ALAVASFGLAESLCRGFDYFPWQMLRSGDFDRLLLRPRSLFVQVAASRFHLHRLVRPVTG 125 A ++ +F LAE RGFD FP M+ +G+FDR+L+RPR QV +S+ L R+ R + Sbjct: 63 ATSLMAFSLAECFGRGFDVFPL-MIGNGEFDRVLVRPRQEIFQVLSSKIELTRIGRLLQS 121 Query: 126 ICAMGWALSELGVSLTPGKLLVLAMALAGGCVMYCGVFVLTSGLAFFTIKGLDWIYLLTN 185 I +A+ GV T K+L L + + G +++ +F++ + +FFTI+GL+++ + T+ Sbjct: 122 IIVFCYAIPASGVIWTWDKILTLFLMIFCGSIIFFCLFLIYASFSFFTIEGLEFMNIFTD 181 Query: 186 ASYQITRCPEPYMPRALKSMFSFVLPMLFISFYPAATVCGWDYPQWXXXXXXXXXXXXXX 245 + R P + + + F+F++P+ +YP + Sbjct: 182 GGKEFGRYPFSIYGKKVLNFFTFIVPLALFQYYPLLYLLDMK-TSVSYMLTPLLGLLFII 240 Query: 246 XXXXVWRFGVRHYKSTGS 263 WRFG+R YKSTGS Sbjct: 241 PSYSFWRFGLRRYKSTGS 258 >A7NKA1.1 A7NKA1_ROSCS Putative uncharacterized protein Length = 265 Score = 125 bits (315), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 87/262 (33%), Positives = 134/262 (51%), Gaps = 5/262 (1%) Query: 7 IYFKYLRMHLLSGMEYK-GWWLMLIQVLVVVISDPIATVLLFSRFGNIGEWTVAHIILVY 65 I + + + S M+YK +WL L+ V + + LL RF IG W + I L+Y Sbjct: 4 ISLRLIGARIRSQMQYKTSFWLDLVGTAVSTGMEFVVLFLLLQRFTLIGGWRLHEIGLLY 63 Query: 66 ALAVASFGLAESLCRGFDYFPWQMLRSGDFDRLLLRPRSLFVQVAASRFHLHRLVRPVTG 125 L SFGLAE + RGFD ++++ GDFDR+L RP S FVQV AS L RL R V G Sbjct: 64 GLTSVSFGLAEMIGRGFDSPFERLIQHGDFDRVLTRPLSSFVQVLASEVQLRRLGRAVQG 123 Query: 126 ICAMGWALSELGVSLTPGKLLVLAMALAGGCVMYCGVFVLTSGLAFFTIKGLDWIYLLTN 185 + ++L+++ TP LL+L +++ G ++Y G+ V+ + + F+TIK + I + T Sbjct: 124 AGVLIFSLAQVEAFWTPVLLLLLLLSILSGALIYVGLIVIGATICFWTIKTPEVINVFTF 183 Query: 186 ASYQITRCPEPYMPRALKSMFSFVLPMLFISFYPAATVCGWDYPQ----WXXXXXXXXXX 241 Q+T P R ++++F + P+ F S+ A + G P W Sbjct: 184 GGQQLTSLPLSIYDRWIRAVFLSIAPVAFASYPAALVILGRSDPHGLPAWIAWCAPVVAS 243 Query: 242 XXXXXXXXVWRFGVRHYKSTGS 263 W+ GVR Y+STG+ Sbjct: 244 VFFAAVLGFWQIGVRRYQSTGT 265 >A9KKD4.1 A9KKD4_CLOPH Putative uncharacterized protein Length = 258 Score = 123 bits (308), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 66/259 (25%), Positives = 136/259 (52%), Gaps = 3/259 (1%) Query: 6 KIYFKYLRMHLLSGMEYK-GWWLMLIQVLVVVISDPIATVLLFSRFGNIGEWTVAHIILV 64 K+Y KY +H S M+YK + L I +V + + LF+RF ++ +T ++L Sbjct: 2 KLYKKYFGIHFRSAMQYKVSFLLTTIGQFLVSFNIFLGIYFLFARFSSVKGYTYQEVMLC 61 Query: 65 YALAVASFGLAESLCRGFDYFPWQMLRSGDFDRLLLRPRSLFVQVAASRFHLHRLVRPVT 124 +A+ + ++ LAE RGFD FP ++ +G+FDR+L+RP+ + +QV S+ R+ R + Sbjct: 62 FAVNIMAYSLAEMFARGFDMFP-SIISNGEFDRILVRPKGILLQVLCSKIEFTRIGRMIQ 120 Query: 125 GICAMGWALSELGVSLTPGKLLVLAMALAGGCVMYCGVFVLTSGLAFFTIKGLDWIYLLT 184 + + + G++ + K++ + + GG +Y +F+L + ++FFTI+G++ + ++T Sbjct: 121 AVIMFFYGVKTSGITWSIDKVITVGFMVIGGTTLYSALFLLYAAVSFFTIEGIEVMNIVT 180 Query: 185 NASYQITRCPEPYMPRALKSMFSFVLPMLFISFYPAATVCGWDYPQWXXXXXXXXXXXXX 244 + + + + P + + + ++++P +YP + G + Sbjct: 181 DGAREYGKYPFDTYGKTVLKIVTYLIPFALFQYYPFLYLLGRN-TNPVLIILPLASAFFY 239 Query: 245 XXXXXVWRFGVRHYKSTGS 263 ++ G++HYKSTGS Sbjct: 240 LPCYLFFQIGLKHYKSTGS 258 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.143 0.479 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,427,853,266 Number of extensions: 53238707 Number of successful extensions: 150350 Number of sequences better than 1.0e-05: 257 Number of HSP's gapped: 150005 Number of HSP's successfully gapped: 257 Length of query: 263 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 130 Effective length of database: 1,807,701,208 Effective search space: 235001157040 Effective search space used: 235001157040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)