BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0031.p (413 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1T903.1 C1T903_9FIRM Sporulation protein YhbH 403 e-110 A4J620.1 A4J620_DESRM Putative uncharacterized protein 394 e-108 C0Z6A3.1 C0Z6A3_BREBN Putative uncharacterized protein yhbH 394 e-107 Q5WHZ3.1 Y1477_BACSK UPF0229 protein ABC1477 376 e-102 B7GM22.1 B7GM22_ANOFW Uncharacterized conserved protein 369 e-100 >C1T903.1 C1T903_9FIRM Sporulation protein YhbH Length = 381 Score = 403 bits (1036), Expect = e-110, Method: Compositional matrix adjust. Identities = 206/404 (50%), Positives = 261/404 (64%), Gaps = 27/404 (6%) Query: 4 MNNRLFMIARDDWSLHRKGEIDQERHKRKVKEAIRHNLADLVSEESIIMPQGDKIIKVPI 63 M+ + ++RDDW+LHRKGEID++RH+ KV+EAI+ NLAD+VSEESIIM G IIKVP+ Sbjct: 1 MSGTKYTVSRDDWTLHRKGEIDRQRHREKVREAIKKNLADIVSEESIIMSDGQNIIKVPV 60 Query: 64 RSLDEPKFRYNYQNEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEQPGEDYYEA 123 RSLDE FR+N++ + E+PG DYYEA Sbjct: 61 RSLDEYHFRFNFKKQ--KHAGQGNGKSKVGDVLGSDPQGAKQGKEKGGAGEEPGNDYYEA 118 Query: 124 ELTIDELAELVFEDLKLPRLKPKAAPDLSIEDIRFDDVRKQGMMSNVDKRRTLFEAIKRQ 183 ++TIDELAE++FEDL LP L PK P+LS + + F D+RK+G+ SN+D++RTL+EA+KR Sbjct: 119 DITIDELAEMIFEDLGLPNLDPKKKPELSSDSVEFKDIRKKGISSNIDRKRTLYEALKRN 178 Query: 184 ALGGGQYMSHAAAVRSDWNPPPEPQYVSSGTMLGPITGDDLRYKTWEDIRKPQSSAVVLA 243 AL G +RS IT +DLRYKTW+ K +SSAVVLA Sbjct: 179 ALSG------KPGLRS-------------------ITQEDLRYKTWDMTVKYESSAVVLA 213 Query: 244 MMDTSGSMGIFEKYVARSFFFWMTRFLRTKYENVQIRFLSHSTDAKEVDEESFFHKGESG 303 MMDTSGSMG FEKY+ARSFFFWM RFLRTKY+NVQI FL+H A E EE FF KG SG Sbjct: 214 MMDTSGSMGPFEKYIARSFFFWMVRFLRTKYQNVQILFLAHHALAHETSEEEFFTKGNSG 273 Query: 304 GTKCSSVYELALRILEADYPPGQHNAYAFHFSDGDNLDSDNPVTVRLAEELLERVNMLGY 363 GTKCSSVYELAL++++ Y P +N YAFHFSDGDNL SDN VRL +E+L+ NM+GY Sbjct: 274 GTKCSSVYELALKVIQERYSPADYNIYAFHFSDGDNLSSDNEKCVRLVQEMLKVCNMVGY 333 Query: 364 GEIRQHVYGGRQLWESMSGIASPAFSRSILKEKEDVLKTLQAFF 407 GEI Y L + + I +P F +++K V L+ FF Sbjct: 334 GEIEGPYYYTSTLRTAFNKINNPEFVTVSIRDKSGVYPALKKFF 377 >A4J620.1 A4J620_DESRM Putative uncharacterized protein Length = 382 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 207/399 (51%), Positives = 258/399 (64%), Gaps = 29/399 (7%) Query: 9 FMIARDDWSLHRKGEIDQERHKRKVKEAIRHNLADLVSEESIIMPQGDKIIKVPIRSLDE 68 F+I R+DWSLHRKGE+DQ RH+ KV+EAI+ NLAD+VSEESII+ G +++KVPIRSLD+ Sbjct: 5 FIITREDWSLHRKGEVDQHRHREKVREAIKKNLADIVSEESIILSDGRRVVKVPIRSLDQ 64 Query: 69 PKFRYNYQNEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEQPGEDYYEAELTID 128 +FRY+ + + E+ G DYYEA++TID Sbjct: 65 YRFRYDARKQ----KHAGQGDGKSKVGDVLGSDPQQSPGNGPGAGEEAGHDYYEADVTID 120 Query: 129 ELAELVFEDLKLPRLKPKAAPDLSIEDIRFDDVRKQGMMSNVDKRRTLFEAIKRQALGGG 188 E+ ++VFEDL LP LK K L+ E I F DVRK G+ SNVD++RT+ E +KR AL G Sbjct: 121 EIEKIVFEDLGLPNLKEKKNKQLASESIEFRDVRKYGIQSNVDRKRTILEVLKRNALKG- 179 Query: 189 QYMSHAAAVRSDWNPPPEPQYVSSGTMLGPITGDDLRYKTWEDIRKPQSSAVVLAMMDTS 248 NP + IT DDLRYKTWE I K +SSAVVLAMMDTS Sbjct: 180 -------------NPG-----------IHGITPDDLRYKTWEVIPKYESSAVVLAMMDTS 215 Query: 249 GSMGIFEKYVARSFFFWMTRFLRTKYENVQIRFLSHSTDAKEVDEESFFHKGESGGTKCS 308 GSMG FEKY+ARSFFFWM RFLRTKY+NV+I FL+H T+AKEV E+ FF KGESGGT+CS Sbjct: 216 GSMGPFEKYIARSFFFWMVRFLRTKYQNVEIVFLAHHTEAKEVSEDEFFTKGESGGTRCS 275 Query: 309 SVYELALRILEADYPPGQHNAYAFHFSDGDNLDSDNPVTVRLAEELLERVNMLGYGEIRQ 368 SVY+LAL ++ YP +N YAFHFSDGDNL SDN +RL EELL + N++GYGEI Sbjct: 276 SVYQLALDLINTRYPKEDYNIYAFHFSDGDNLSSDNEKCIRLIEELLNKCNLVGYGEIEG 335 Query: 369 HVYGGRQLWESMSGIASPAFSRSILKEKEDVLKTLQAFF 407 Y L S+ I SP+F +++K V L+AFF Sbjct: 336 PYYYTSTLNSSLKKINSPSFVSVTIRDKTGVYPALKAFF 374 >C0Z6A3.1 C0Z6A3_BREBN Putative uncharacterized protein yhbH Length = 385 Score = 394 bits (1011), Expect = e-107, Method: Compositional matrix adjust. Identities = 202/404 (50%), Positives = 264/404 (65%), Gaps = 27/404 (6%) Query: 9 FMIARDDWSLHRKGEIDQERHKRKVKEAIRHNLADLVSEESIIMPQGDKIIKVPIRSLDE 68 F+++R+DWSLHRKG DQ RH+ KVKEAI+ NL DLVSEE+IIM G ++IK+PIRSLDE Sbjct: 7 FIVSREDWSLHRKGYQDQNRHQEKVKEAIKKNLPDLVSEENIIMSNGREVIKIPIRSLDE 66 Query: 69 PKFRYNYQNEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEQPGEDYYEAELTID 128 + R+N+Q +QPG DYYEAE++++ Sbjct: 67 YRLRFNFQKGKHGGQGKGNSKVGDVVARDGDPAQGPGKGQGAG--DQPGVDYYEAEISVE 124 Query: 129 ELAELVFEDLKLPRLKPKAAPDLSIEDIRFDDVRKQGMMSNVDKRRTLFEAIKRQALGGG 188 EL E++F +L+LP L+ K + +E+ RF+DVRK+G+M N+DK+RTL AI+R AL G Sbjct: 125 ELQEMLFAELELPNLQQKDEEQIVVEETRFNDVRKKGLMGNIDKKRTLIAAIRRNALAGY 184 Query: 189 QYMSHAAAVRSDWNPPPEPQYVSSGTMLGPITGDDLRYKTWEDIRKPQSSAVVLAMMDTS 248 M LG IT DDLR+KTWE+I KP S+AV++AMMDTS Sbjct: 185 SDME-----------------------LG-ITDDDLRFKTWEEIIKPHSNAVIIAMMDTS 220 Query: 249 GSMGIFEKYVARSFFFWMTRFLRTKYENVQIRFLSHSTDAKEVDEESFFHKGESGGTKCS 308 GSMG+FEKY+ARSFFFWM RFLRTKYE V+I F++H TDAKEV E++FF KGESGGT CS Sbjct: 221 GSMGVFEKYIARSFFFWMVRFLRTKYEKVEIVFIAHHTDAKEVTEDTFFSKGESGGTICS 280 Query: 309 SVYELALRILEADYPPGQHNAYAFHFSDGDNLDSDNPVTVRLAEELLERVNMLGYGEIRQ 368 S Y+ AL I+++ YP Q+N Y FHFSDGDNL SDN V+L +EL+ER NM GYGE+ Q Sbjct: 281 SAYKKALEIIDSRYPTSQYNIYPFHFSDGDNLTSDNERCVKLVKELIERCNMFGYGEVNQ 340 Query: 369 HVYGGRQLWESMSGIASPAFSRSILKEKEDVLKTLQAFFGEELA 412 + L + I P F +++EK +V K L+ FFG++ A Sbjct: 341 YNRHS-TLMSAYRHIKEPKFMHCVIREKGEVYKALRTFFGKKEA 383 >Q5WHZ3.1 Y1477_BACSK UPF0229 protein ABC1477 Length = 390 Score = 376 bits (965), Expect = e-102, Method: Compositional matrix adjust. Identities = 195/403 (48%), Positives = 246/403 (61%), Gaps = 26/403 (6%) Query: 5 NNRLFMIARDDWSLHRKGEIDQERHKRKVKEAIRHNLADLVSEESIIMPQGDKIIKVPIR 64 N R F I++++WSLHRKG DQ RH+ KV++AI+ NL DLVSEE+I+M G +IK+PIR Sbjct: 5 NGRQFTISQENWSLHRKGFQDQRRHQEKVRDAIKKNLPDLVSEENIVMSNGKDVIKIPIR 64 Query: 65 SLDEPKFRYNYQNEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEQPGEDYYEAE 124 SLDE K RYNY +Q GEDY EAE Sbjct: 65 SLDEYKIRYNYDKNQHVGQGKGDSKIGDIVARDPNGDKQAGAGKGSGAGDQAGEDYNEAE 124 Query: 125 LTIDELAELVFEDLKLPRLKPKAAPDLSIEDIRFDDVRKQGMMSNVDKRRTLFEAIKRQA 184 ++I EL E++F +L LP L+ K +L +EDI F+D+RK+G+M NVDKRRT+ AIKR A Sbjct: 125 VSIVELEEMLFSELALPNLQKKEEQELVVEDIAFNDIRKKGLMGNVDKRRTILAAIKRNA 184 Query: 185 LGGGQYMSHAAAVRSDWNPPPEPQYVSSGTMLGPITGDDLRYKTWEDIRKPQSSAVVLAM 244 L G + PI DDLR+KTW + +P+S AVV+AM Sbjct: 185 LNGK-------------------------ANIMPIYNDDLRFKTWTETIRPESKAVVIAM 219 Query: 245 MDTSGSMGIFEKYVARSFFFWMTRFLRTKYENVQIRFLSHSTDAKEVDEESFFHKGESGG 304 MDTSGSMG FEKY+ARSFFFWMTRFLRTKYE V+I F++H T+AK V EE FF KGESGG Sbjct: 220 MDTSGSMGRFEKYMARSFFFWMTRFLRTKYETVEIEFIAHHTEAKVVSEEDFFSKGESGG 279 Query: 305 TKCSSVYELALRILEADYPPGQHNAYAFHFSDGDNLDSDNPVTVRLAEELLERVNMLGYG 364 T CSS Y AL +++ Y P +N Y FHFSDGDNL SDN ++L +L++R N+ GYG Sbjct: 280 TICSSAYRKALELIDEKYNPRAYNIYPFHFSDGDNLTSDNARCLKLVNQLMDRSNLFGYG 339 Query: 365 EIRQHVYGGRQLWESMSGIASPAFSRSILKEKEDVLKTLQAFF 407 E+ Q+ L + I P F ILKEK DV L+ FF Sbjct: 340 EVNQYSRHS-TLMSAYKNITDPRFMHYILKEKGDVYHALKFFF 381 >B7GM22.1 B7GM22_ANOFW Uncharacterized conserved protein Length = 405 Score = 369 bits (946), Expect = e-100, Method: Compositional matrix adjust. Identities = 190/405 (46%), Positives = 249/405 (61%), Gaps = 26/405 (6%) Query: 6 NRLFMIARDDWSLHRKGEIDQERHKRKVKEAIRHNLADLVSEESIIMPQGDKIIKVPIRS 65 +R F+++++DWSLHRKG DQ+RH+ KVKEAI++NL DL++EESIIM G +IK+PIRS Sbjct: 21 SRNFIVSKEDWSLHRKGHDDQKRHEEKVKEAIKNNLGDLITEESIIMSNGRDVIKIPIRS 80 Query: 66 LDEPKFRYNYQNEPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEQPGEDYYEAEL 125 LDE K RYNY + G+DYYEAE+ Sbjct: 81 LDEYKIRYNYDKNKHVGQGDGDSQVGDVIARDGSGDSQKGPEKGQGAGDLAGQDYYEAEV 140 Query: 126 TIDELAELVFEDLKLPRLKPKAAPDLSIEDIRFDDVRKQGMMSNVDKRRTLFEAIKRQAL 185 ++ EL E +F L+LP LK K D ++ I F+D+R+ G+M N+DK++T+ A KR AL Sbjct: 141 SLMELEEALFSQLELPDLKKKQHADHVVQHIEFNDIRRTGLMGNIDKKKTMMAAFKRNAL 200 Query: 186 GGGQYMSHAAAVRSDWNPPPEPQYVSSGTMLGPITGDDLRYKTWEDIRKPQSSAVVLAMM 245 G NP PI +DL++KTW ++ KP S AVV+AMM Sbjct: 201 SG--------------NPS-----------FYPIYPEDLKFKTWNEVIKPDSKAVVIAMM 235 Query: 246 DTSGSMGIFEKYVARSFFFWMTRFLRTKYENVQIRFLSHSTDAKEVDEESFFHKGESGGT 305 DTSGSMG++EKY+ARSFFFWMTRFLRTKYE V I F++H T+AK V EE FF KGESGGT Sbjct: 236 DTSGSMGVWEKYMARSFFFWMTRFLRTKYETVDIVFIAHHTEAKIVTEEEFFTKGESGGT 295 Query: 306 KCSSVYELALRILEADYPPGQHNAYAFHFSDGDNLDSDNPVTVRLAEELLERVNMLGYGE 365 CSS Y AL ++E Y P ++N Y FHFSDGDNL SDN V+L +EL++ NM GYGE Sbjct: 296 ICSSAYRKALEVIETKYSPTKYNIYPFHFSDGDNLTSDNARCVKLVQELMKVSNMFGYGE 355 Query: 366 IRQHVYGGRQLWESMSGIASPAFSRSILKEKEDVLKTLQAFFGEE 410 + Q+ L + I F ILK+K DVL+ ++ FF +E Sbjct: 356 VNQYNRHS-TLMSAYKHIKDEKFRYYILKQKSDVLQAMRTFFRKE 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,378,788,661 Number of extensions: 96561421 Number of successful extensions: 218947 Number of sequences better than 1.0e-05: 513 Number of HSP's gapped: 218582 Number of HSP's successfully gapped: 617 Length of query: 413 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 275 Effective length of database: 1,760,596,133 Effective search space: 484163936575 Effective search space used: 484163936575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)