BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0030.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GEX1.1 B7GEX1_ANOFW Stage V sporulation protein R required for... 384 e-105 Q5WHX0.1 Q5WHX0_BACSK Stage V sporulation protein R 376 e-102 C0Z7A3.1 C0Z7A3_BREBN Stage V sporulation protein R 374 e-102 Q67Q88.1 Q67Q88_SYMTH Stage V sporulation protein R 351 4e-95 B1I269.1 B1I269_DESAP SpoVR family protein 337 8e-91 >B7GEX1.1 B7GEX1_ANOFW Stage V sporulation protein R required for dehydratation of the spore core and assembly of the coat Length = 472 Score = 384 bits (985), Expect = e-105, Method: Compositional matrix adjust. Identities = 189/373 (50%), Positives = 247/373 (66%), Gaps = 54/373 (14%) Query: 1 MTDNETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKA 60 M +E LE+++ +T +A GLDF+ MRY++CP +++Y+ GAYGMPTRF+HWSFGK Sbjct: 1 MKRDEQKALEKAIAEITEIAEGFGLDFYEMRYEICPADIIYTFGAYGMPTRFSHWSFGKQ 60 Query: 61 YHRMKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGG 120 +H+MK YD GLSKIYELVINSDPCYAFLL++NTL+QNKLIVAHVL HSDFFKNN F Sbjct: 61 FHKMKLHYDLGLSKIYELVINSDPCYAFLLNTNTLIQNKLIVAHVLAHSDFFKNNVRFSN 120 Query: 121 TDRNMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSAR------------ 168 T R+M+ MA AERI + EYG EVE+FLDA +AIQEHIDP R Sbjct: 121 TKRDMVESMAATAERIKHYEHEYGKQEVEKFLDAVLAIQEHIDPSLLRPKLAWTLADTEV 180 Query: 169 ------SGRPGGFREIGG--------------------EVKDVIGYIAAESRYLEEWQRE 202 + +P + ++ KD++ +I SR L+EWQR+ Sbjct: 181 YEEDDEAPKPSPYDDLWSLDEKRKTPSTRKKRRKFPPQPEKDLLLFIEEYSRELDEWQRD 240 Query: 203 ILYMLRAEMLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPG 262 IL M+R EMLYFWPQ+ETKIMNEGWA+YWH +++RE+DL+ E IEF+K+N+GV+QPS Sbjct: 241 ILTMMREEMLYFWPQLETKIMNEGWATYWHQRILREMDLTSDEAIEFAKLNAGVVQPSRT 300 Query: 263 RINPYYLGLMLLRHIEKR----------------EGLQALFEIREIESDVSFIRNYLTRE 306 INPYYLGL + IEKR G +FE+RE+ESD+SF+RNYLT+E Sbjct: 301 GINPYYLGLKIFEDIEKRWDNPTEEMKKLGVKPGSGRAKIFEVRELESDISFLRNYLTKE 360 Query: 307 LAEEMDLFVFKKE 319 L D+++F+K+ Sbjct: 361 LVMREDMYLFQKQ 373 >Q5WHX0.1 Q5WHX0_BACSK Stage V sporulation protein R Length = 463 Score = 376 bits (965), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/365 (50%), Positives = 243/365 (66%), Gaps = 47/365 (12%) Query: 1 MTDNETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKA 60 M+DN +L+ ++E +T +A GLDF+ MRY++CP +++Y+ GAYGMPTR+ HWSFGK Sbjct: 1 MSDNRK-ELQYAIEEITEIAKGFGLDFYEMRYEICPADIIYTFGAYGMPTRYNHWSFGKQ 59 Query: 61 YHRMKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGG 120 YH+MK +YD GLSKIYELVINSDPCYAFLLDSN+L+QNKLIVAHVL H DFFKNN F Sbjct: 60 YHKMKLQYDLGLSKIYELVINSDPCYAFLLDSNSLIQNKLIVAHVLAHCDFFKNNIRFSN 119 Query: 121 TDRNMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDP----------FSARSG 170 T R+M+ M+ AER+ + +G ++VE FLDA +AIQEHIDP + Sbjct: 120 TRRDMVESMSATAERVAHYEHIHGKEQVENFLDAVLAIQEHIDPGLVKPVESSELTFEKP 179 Query: 171 RPGGFREI-------------------GGEVKDVIGYIAAESRYLEEWQREILYMLRAEM 211 R + ++ KDV+ +I SR LE WQR+IL M+R EM Sbjct: 180 RASEYEDLWNLEEQPKKEAAPKKKRFPESPEKDVLRFIEHHSRELEPWQRDILTMMREEM 239 Query: 212 LYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPGRINPYYLGL 271 LYFWPQ+ETKIMNEGWAS+WH +++RE+DL+ E +EF+K+N+ V+QPS INPYYLGL Sbjct: 240 LYFWPQLETKIMNEGWASFWHARIIREMDLTSEEAVEFAKLNAAVVQPSKTSINPYYLGL 299 Query: 272 MLLRHIEKR-----------------EGLQALFEIREIESDVSFIRNYLTRELAEEMDLF 314 + IE+R G + +FE+REIESD SFIRNYLT+EL D++ Sbjct: 300 KIFEDIEQRYNEPTEEMQRKQGIKPGSGREKIFEVREIESDTSFIRNYLTKELVHREDMY 359 Query: 315 VFKKE 319 +F+K+ Sbjct: 360 LFQKQ 364 >C0Z7A3.1 C0Z7A3_BREBN Stage V sporulation protein R Length = 473 Score = 374 bits (961), Expect = e-102, Method: Compositional matrix adjust. Identities = 183/374 (48%), Positives = 251/374 (67%), Gaps = 55/374 (14%) Query: 1 MTDNETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKA 60 MT++E +LE S++ +T +A LDF+ MRY++CP +++Y+ GAYGMPTRF+HWSFGK+ Sbjct: 1 MTNDERKELERSIDEITEIAKGFNLDFYPMRYEICPSDIIYTFGAYGMPTRFSHWSFGKS 60 Query: 61 YHRMKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGG 120 ++RMK +YD LSKIYELVINS+PCYAFLLD N+L+QNKLIVAHVL H DFFKNN F Sbjct: 61 FYRMKLQYDLNLSKIYELVINSNPCYAFLLDGNSLIQNKLIVAHVLAHCDFFKNNVRFSN 120 Query: 121 TDRNMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPF--------------- 165 T+R+M+ MA ERI ++ EYG + VE LDA +AIQEHIDP Sbjct: 121 TNRDMVESMAASGERIRQYEIEYGKEAVENLLDASLAIQEHIDPSLLRPKLRWKREPEEA 180 Query: 166 --SARSGRPGGFREIGG---------------------EVKDVIGYIAAESRYLEEWQRE 202 R+ R + ++ G KD++ +I S L++WQR+ Sbjct: 181 NKKTRNERNSPYDDLWGLDRKAKEEAEPVKAPRKFPPEPEKDLLLFIMEYSNILDDWQRD 240 Query: 203 ILYMLRAEMLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPG 262 +L ++R EMLYFWPQMETKIMNEGWA+YWH++++RE++L++SE IEF+K++S VIQPS Sbjct: 241 VLTIIRDEMLYFWPQMETKIMNEGWATYWHMRILREMELTESETIEFAKLHSSVIQPSTT 300 Query: 263 RINPYYLGLMLLRHIEKR-----------------EGLQALFEIREIESDVSFIRNYLTR 305 INPY+LGL +L IEKR EG +FE+RE+ESD+SFIRN+LT+ Sbjct: 301 SINPYHLGLKILEDIEKRWDNPTKEEQERFGRKPGEGRAKIFEVRELESDISFIRNFLTK 360 Query: 306 ELAEEMDLFVFKKE 319 +L ++DL++F K+ Sbjct: 361 DLVSDLDLYMFGKQ 374 >Q67Q88.1 Q67Q88_SYMTH Stage V sporulation protein R Length = 408 Score = 351 bits (901), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 163/312 (52%), Positives = 226/312 (72%), Gaps = 9/312 (2%) Query: 8 QLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKAYHRMKSE 67 +L E+VE + A GLDFF +R+++CPP+VLY+ GAYGMPTRF+HWSFGKAYH +KS+ Sbjct: 7 RLGEAVEAVVRRARAAGLDFFPLRFELCPPDVLYTFGAYGMPTRFSHWSFGKAYHLLKSQ 66 Query: 68 YDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGGTDRNMIN 127 GL+KIYELVINSDPC+AFLL+SN+LL+N L+ AHVL H+DFFK+N F T+R M++ Sbjct: 67 AKLGLTKIYELVINSDPCHAFLLESNSLLENTLVAAHVLAHADFFKHNWRFQSTNRRMLH 126 Query: 128 RMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSARSGRPGGFREIGGEVKDVIG 187 M+ FAER+ R+ + G VERFLDA +A+ E IDPF P +D+ Sbjct: 127 SMSAFAERVRRYEERLGRGPVERFLDAALAVAEQIDPFPGPRPAPQ---------RDLPL 177 Query: 188 YIAAESRYLEEWQREILYMLRAEMLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEII 247 ++ + L +W+R+IL LR E LYF PQMETKI+NEGWA++WH++L+RELDL ++ + Sbjct: 178 FLLERAPELADWERDILASLREEALYFRPQMETKILNEGWATFWHVRLLRELDLPPAQAV 237 Query: 248 EFSKMNSGVIQPSPGRINPYYLGLMLLRHIEKREGLQALFEIREIESDVSFIRNYLTREL 307 EF++M++ VI P P ++NPY LG LL IE R G + LFE+R +E+DVS +RNYLT EL Sbjct: 238 EFARMHAAVIAPHPHQLNPYRLGYHLLESIEARYGRERLFEVRTLETDVSLVRNYLTPEL 297 Query: 308 AEEMDLFVFKKE 319 + ++L V+ ++ Sbjct: 298 CQALNLHVYAQQ 309 >B1I269.1 B1I269_DESAP SpoVR family protein Length = 419 Score = 337 bits (864), Expect = 8e-91, Method: Compositional matrix adjust. Identities = 163/322 (50%), Positives = 224/322 (69%), Gaps = 7/322 (2%) Query: 1 MTDNETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKA 60 M D E+ LE+ +E + A ++GLD M +++CPPEVLYS GAY MPTRF HWSFGKA Sbjct: 1 MHDMES--LEKDIEEIMAAARQYGLDHRDMHFEICPPEVLYSFGAYRMPTRFGHWSFGKA 58 Query: 61 YHRMKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGG 120 Y R+K+EYDH LS++YE+VIN+DPCYAFLL NT +QN+L++AH L HSDFFK+NA F Sbjct: 59 YQRIKTEYDHNLSRMYEMVINTDPCYAFLLQGNTRIQNRLVIAHALAHSDFFKHNAYFAR 118 Query: 121 T-DRNMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHID-PFSARSGR--PGGFR 176 T R ++ M + A RI + Q YG + VE+FLDA +A+QEH+D S +GR P G Sbjct: 119 TAPRTVLESMTVAAGRIRNYEQAYGRERVEQFLDAALAVQEHVDYHHSPGTGRRYPAG-E 177 Query: 177 EIGGEVKDVIGYIAAESRYLEEWQREILYMLRAEMLYFWPQMETKIMNEGWASYWHLKLV 236 DV+G+IA LE WQR+IL ++R E LYF PQ+ TK+ NEGWA++WHLK++ Sbjct: 178 SASHSKNDVLGFIARFGHGLESWQRDILAVVREESLYFRPQLATKLCNEGWATFWHLKIM 237 Query: 237 RELDLSDSEIIEFSKMNSGVIQPSPGRINPYYLGLMLLRHIEKREGLQALFEIREIESDV 296 L+L++ E ++F++M++ ++QPS R+NPY LGL + I++R G +FE R D Sbjct: 238 HHLELTEEETVDFARMHAQLVQPSRLRVNPYLLGLSIFSDIDRRWGRDTVFEARATCDDP 297 Query: 297 SFIRNYLTRELAEEMDLFVFKK 318 +RNYLT EL +MDLFVF++ Sbjct: 298 GLLRNYLTEELVRKMDLFVFRR 319 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,995,118,323 Number of extensions: 86699938 Number of successful extensions: 181366 Number of sequences better than 1.0e-05: 532 Number of HSP's gapped: 180107 Number of HSP's successfully gapped: 943 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)