BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0025.p (401 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3BJX2.1 C3BJX2_9BACI Permease (Major facilitator superfamily) 344 1e-92 C3B385.1 C3B385_BACMY Permease (Major facilitator superfamily) 344 1e-92 C3AL28.1 C3AL28_BACMY Permease (Major facilitator superfamily) 344 1e-92 C2YTE4.1 C2YTE4_BACCE Permease (Major facilitator superfamily) 280 2e-73 Q0W1D9.1 Q0W1D9_UNCMA Permease (Major facilitator superfamily) 268 6e-70 >C3BJX2.1 C3BJX2_9BACI Permease (Major facilitator superfamily) Length = 390 Score = 344 bits (882), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 178/360 (49%), Positives = 249/360 (69%) Query: 9 QKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTEIGLLFGSYAIALFIATPVFGA 68 QK++L+VV +A+F DMLIYG++VPILP+YA LGASQTEIG LFGSYAI L +ATP+ G Sbjct: 11 QKASLWVVAVAVFTDMLIYGMIVPILPRYAETLGASQTEIGFLFGSYAITLLLATPILGV 70 Query: 69 LSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLY 128 +SD++GRR PM+ +W+ TWT+GLA+LAD++ Sbjct: 71 VSDKVGRRLPMILGLFGLAAATLLFGLANSFWLLVLARMLQGISAAATWTAGLALLADVF 130 Query: 129 PSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRD 188 P +ERGKAMGLALSGQAAG+LLGP++GG +YQ GGY LPF+ AA +A+ DG+LRI LL + Sbjct: 131 PLQERGKAMGLALSGQAAGMLLGPTIGGALYQWGGYHLPFIIAASIALIDGILRITLLHN 190 Query: 189 LPERKSGTFLSSFGLLRNRSLLLISGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGML 248 P+ S +S +L RSL ++ G++I+GSA+PS LEPTLPL+LQ+ + PG IG+L Sbjct: 191 EPKSSSNQRISYRSILNMRSLFIMIGIIILGSALPSALEPTLPLYLQDTLHLSPGTIGLL 250 Query: 249 FAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGI 308 FA+PTL+Y P IG LS++IG K+ + +G+A+ A+ P + + ++ L +LGI Sbjct: 251 FAVPTLAYGFTAPIIGTLSTKIGRKQTMAVGIALAALCFPFTVIVSHTALEIFVLAILGI 310 Query: 309 SMGMVLAPCLPALADISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLSY 368 S ++LAP LP L +++++G+ +YGI FAIYNTAYS GM GP+ SG L+D G ++ Sbjct: 311 SFSLLLAPALPELTYLADKNGVHAYGILFAIYNTAYSIGMFFGPMLSGSLSDLFGFTNAF 370 >C3B385.1 C3B385_BACMY Permease (Major facilitator superfamily) Length = 390 Score = 344 bits (882), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 178/360 (49%), Positives = 249/360 (69%) Query: 9 QKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTEIGLLFGSYAIALFIATPVFGA 68 QK++L+VV +A+F DMLIYG++VPILP+YA LGASQTEIG LFGSYAI L +ATP+ G Sbjct: 11 QKASLWVVAVAVFTDMLIYGMIVPILPRYAETLGASQTEIGFLFGSYAITLLLATPILGV 70 Query: 69 LSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLY 128 +SD++GRR PM+ +W+ TWT+GLA+LAD++ Sbjct: 71 VSDKVGRRLPMILGLFGLAAATLLFGLANSFWLLVLARMLQGISAAATWTAGLALLADVF 130 Query: 129 PSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRD 188 P +ERGKAMGLALSGQAAG+LLGP++GG +YQ GGY LPF+ AA +A+ DG+LRI LL + Sbjct: 131 PLQERGKAMGLALSGQAAGMLLGPTIGGALYQWGGYHLPFIIAASIALIDGILRITLLHN 190 Query: 189 LPERKSGTFLSSFGLLRNRSLLLISGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGML 248 P+ S +S +L RSL ++ G++I+GSA+PS LEPTLPL+LQ+ + PG IG+L Sbjct: 191 EPKSSSNQRISYRSILNMRSLFIMIGIIILGSALPSALEPTLPLYLQDTLHLSPGTIGLL 250 Query: 249 FAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGI 308 FA+PTL+Y P IG LS++IG K+ + +G+A+ A+ P + + ++ L +LGI Sbjct: 251 FAVPTLAYGFTAPIIGTLSTKIGRKQTMAVGIALAALCFPFTVIVSHTALEIFVLAILGI 310 Query: 309 SMGMVLAPCLPALADISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLSY 368 S ++LAP LP L +++++G+ +YGI FAIYNTAYS GM GP+ SG L+D G ++ Sbjct: 311 SFSLLLAPALPELTYLADKNGVHAYGILFAIYNTAYSIGMFFGPMLSGSLSDLFGFTNAF 370 >C3AL28.1 C3AL28_BACMY Permease (Major facilitator superfamily) Length = 390 Score = 344 bits (882), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 178/360 (49%), Positives = 249/360 (69%) Query: 9 QKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTEIGLLFGSYAIALFIATPVFGA 68 QK++L+VV +A+F DMLIYG++VPILP+YA LGASQTEIG LFGSYAI L +ATP+ G Sbjct: 11 QKASLWVVAVAVFTDMLIYGMIVPILPRYAETLGASQTEIGFLFGSYAITLLLATPILGV 70 Query: 69 LSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLY 128 +SD++GRR PM+ +W+ TWT+GLA+LAD++ Sbjct: 71 VSDKVGRRLPMILGLFGLAAATLLFGLANSFWLLVLARMLQGISAAATWTAGLALLADVF 130 Query: 129 PSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRD 188 P +ERGKAMGLALSGQAAG+LLGP++GG +YQ GGY LPF+ AA +A+ DG+LRI LL + Sbjct: 131 PLQERGKAMGLALSGQAAGMLLGPTIGGALYQWGGYHLPFIIAASIALIDGILRITLLHN 190 Query: 189 LPERKSGTFLSSFGLLRNRSLLLISGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGML 248 P+ S +S +L RSL ++ G++I+GSA+PS LEPTLPL+LQ+ + PG IG+L Sbjct: 191 EPKSSSNQRISYRSILNMRSLFIMIGIIILGSALPSALEPTLPLYLQDTLHLSPGTIGLL 250 Query: 249 FAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGI 308 FA+PTL+Y P IG LS++IG K+ + +G+A+ A+ P + + ++ L +LGI Sbjct: 251 FAVPTLAYGFTAPIIGTLSTKIGRKQTMAVGIALAALCFPFTVIVSHTALEIFVLAILGI 310 Query: 309 SMGMVLAPCLPALADISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLSY 368 S ++LAP LP L +++++G+ +YGI FAIYNTAYS GM GP+ SG L+D G ++ Sbjct: 311 SFSLLLAPALPELTYLADKNGVHAYGILFAIYNTAYSIGMFFGPMLSGSLSDLFGFTNAF 370 >C2YTE4.1 C2YTE4_BACCE Permease (Major facilitator superfamily) Length = 400 Score = 280 bits (716), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 143/357 (40%), Positives = 215/357 (60%) Query: 11 SALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTEIGLLFGSYAIALFIATPVFGALS 70 + L V+ +A+F+DML+Y LVVPI+P Y S GASQT +G+L YA TP+ G +S Sbjct: 13 TGLLVIAMAVFIDMLLYSLVVPIVPLYVSKFGASQTTVGILISCYAFTFIFFTPILGVVS 72 Query: 71 DRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLYPS 130 D+ GRRG MLW ++ TWT+GLA++ DLYP+ Sbjct: 73 DKFGRRGSMLWGMVVLIASTLMFAFANNMYILILARLLQGIAAAATWTAGLALITDLYPA 132 Query: 131 EERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRDLP 190 +RGKA+G ++ +AG LLG +GG +Y+ GGY+LPFL AA +A+ DG+ R+FLL D P Sbjct: 133 NKRGKALGTVITFMSAGTLLGAPVGGLLYEWGGYQLPFLLAAGIALLDGLARMFLLEDPP 192 Query: 191 ERKSGTFLSSFGLLRNRSLLLISGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGMLFA 250 + K + + + ++ +L I GVV++GS+ S+LEPTLP++L E A IG LF Sbjct: 193 KDKETSSVDIAKIFKDFEVLKIFGVVLLGSSAISILEPTLPIYLMENLGASTVHIGFLFG 252 Query: 251 IPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGISM 310 I TL+Y LA+P G LS + G ++IGL ++A+ LP +P+++ ++ L LG + Sbjct: 253 ITTLAYGLASPIAGWLSDKSGTFHIMMIGLLILAVCLPFVTIPNTLVLEGVVLFFLGAAA 312 Query: 311 GMVLAPCLPALADISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLS 367 G+VL P LP LA+ ++ G +Y FA++N AYS GM++GP+ G D + ++ Sbjct: 313 GLVLTPTLPELANSVDRLGGGAYATAFAVFNVAYSVGMMIGPIFGGFFADMFNVSIA 369 >Q0W1D9.1 Q0W1D9_UNCMA Permease (Major facilitator superfamily) Length = 410 Score = 268 bits (686), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 159/388 (40%), Positives = 230/388 (59%), Gaps = 6/388 (1%) Query: 8 KQKSALFVVGIAIFLDMLIYGLVVPILPKYASALGASQTEIGLLFGSYAIALFIATPVFG 67 QK AL VV IAIF DMLIY ++VPILP+YAS LGASQ IGLLF SYAI +A PV G Sbjct: 14 NQKLALLVVFIAIFTDMLIYCMIVPILPEYASGLGASQQMIGLLFASYAITFLLAAPVVG 73 Query: 68 ALSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAILADL 127 LSDR+GR+ PML + M TWT+GLA+L+DL Sbjct: 74 VLSDRVGRKLPMLVGLLGLFGSTLLFAFSSDLTMLFVARALQGVSAGATWTAGLALLSDL 133 Query: 128 YPSEERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLR 187 +P + R +A+G+ +SG AG LLGP GG +Y+ GGY LPFL AA L + DG+ R+ LL+ Sbjct: 134 FPPKMRQQAIGIGISGSFAGTLLGPLFGGALYEYGGYALPFLAAAGLVLIDGIARLLLLK 193 Query: 188 DLPERKSGTFLSSFGLLRNRSLLLISGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGM 247 D P + +S +L+NR++LL++GV+++ SA ++LEP LPL+L + P IG+ Sbjct: 194 DPPRLPAEQQVSLKSMLKNRTILLMAGVIVLVSAATALLEPVLPLYLSDVLKVSPTMIGL 253 Query: 248 LFAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLG 307 LFA+ ++ +A+P K++ R G K+ ++ GL A+ LP AL S ++ + LG Sbjct: 254 LFAVTVVASLIASPLSYKVAERFGRKRVIIAGLICSALILPTLALSGSFILEVVLMAALG 313 Query: 308 ISMGMVLAPCLPALADISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLS 367 + + LA + D+ ++ G YG +AIYN A SFGM+ GP+ G+L + G+ + Sbjct: 314 GVLAIGLASVPLEMTDVIDRLGQGGYGAVYAIYNLALSFGMMAGPIAGGLLAGSFGIT-A 372 Query: 368 YLSVGCA-----ALLYMFIMLPSFRRTE 390 L +G A A+L + +M S + +E Sbjct: 373 GLGIGGAFMIGYAMLLLIVMRGSGKASE 400 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,131,353,747 Number of extensions: 87782236 Number of successful extensions: 447367 Number of sequences better than 1.0e-05: 3787 Number of HSP's gapped: 447681 Number of HSP's successfully gapped: 4101 Length of query: 401 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 263 Effective length of database: 1,760,596,133 Effective search space: 463036782979 Effective search space used: 463036782979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)