BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0024.p (463 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D976.1 B1D976_9BACL MATE efflux family protein 591 e-167 B3K6X2.1 B3K6X2_9BACI MATE efflux family protein 578 e-163 C1PA22.1 C1PA22_BACCO MATE efflux family protein 454 e-126 C2BXR7.1 C2BXR7_LISGR MATE efflux family protein 390 e-106 C3RHV7.1 C3RHV7_9MOLU MATE efflux family protein 255 5e-66 >B1D976.1 B1D976_9BACL MATE efflux family protein Length = 465 Score = 591 bits (1523), Expect = e-167, Method: Compositional matrix adjust. Identities = 289/461 (62%), Positives = 360/461 (78%), Gaps = 1/461 (0%) Query: 3 EPKVNVKQGRLFLDKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXX 62 P++ K FL+K+F+G SIDYRQII+LF+PIL+DQAF+VGLN+VNT Sbjct: 5 RPRLRGKH-EAFLEKHFTGVSIDYRQIIALFIPILIDQAFLVGLNLVNTAMVSSSGVDAV 63 Query: 63 XXXNMIDSLNIFLISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGL 122 NM+DSLNIFLISVFIAVSTGGTVVVAQYKGSGN M L+AFAI L Sbjct: 64 SAVNMVDSLNIFLISVFIAVSTGGTVVVAQYKGSGNHQMVSKSAASSVSSVTLVAFAISL 123 Query: 123 FGIVFHGPLLHLLFGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLV 182 +VFH +L+LLFGAASPEV RTYLIGS LSY+GIA+MEAVCGALRGIGR+R SL+ Sbjct: 124 IVVVFHSQMLNLLFGAASPEVFQEGRTYLIGSGLSYVGIAMMEAVCGALRGIGRSRASLM 183 Query: 183 LSLIMNFSYVLLNFVFINGLHMGVFGMTIAINASRYLAAVCSLIYLFRLDSSLRVKIRDL 242 LSL+MN +YVLLN VFIN ++MGV G+ I++N SRY AA+ +++YL ++D+SLR+++RD+ Sbjct: 184 LSLVMNLTYVLLNIVFINLMNMGVLGLAISVNISRYAAAILAIVYLVKIDTSLRLRLRDM 243 Query: 243 LALNWTMIKRIMFIGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIP 302 L++N++M+++I+F+GLPFAAEQMFFNGGKI+TQ F+V LGT+AIATNAIS SLA V+QIP Sbjct: 244 LSVNFSMLRKILFVGLPFAAEQMFFNGGKIVTQTFVVGLGTFAIATNAISGSLANVLQIP 303 Query: 303 ANALSLTLITVVGQCMGSRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFHPP 362 +NAL++T ITV+GQCMG RN +DARKF + P+F PLV LFHPP Sbjct: 304 SNALAITAITVIGQCMGQRNIEDARKFTRSFLWLSTACLFVMACLMLPLFYPLVGLFHPP 363 Query: 363 EEIVRDIFIVFLINSIAQIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGYIL 422 EIV DI I+ +IN++ Q+ WS+SFI PSALRAAGDSKFTSVVS+LSMWLFR+VLGY+L Sbjct: 364 AEIVDDILIITIINALMQVVFWSISFIMPSALRAAGDSKFTSVVSLLSMWLFRIVLGYLL 423 Query: 423 GIVLNMGIIGVWLAMNCEWGVRGFIFLRRFLGEKWVQHKVI 463 IV + GIIGVWLAMN EWGVRG IFL RF G+KW +H++I Sbjct: 424 AIVFDFGIIGVWLAMNLEWGVRGAIFLWRFRGKKWYRHRLI 464 >B3K6X2.1 B3K6X2_9BACI MATE efflux family protein Length = 453 Score = 578 bits (1489), Expect = e-163, Method: Compositional matrix adjust. Identities = 281/449 (62%), Positives = 346/449 (77%) Query: 15 LDKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXNMIDSLNIF 74 L + SGES+DYRQI +LF PIL+DQAF++GLN+VNT NM+DSLNIF Sbjct: 5 LQHHLSGESMDYRQIFALFFPILIDQAFIIGLNLVNTAMISSSGVSAVSAVNMVDSLNIF 64 Query: 75 LISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIVFHGPLLHL 134 LI+VF+AV+TGGTVVVAQYKGSGN +M ++A I L I H P+L+L Sbjct: 65 LINVFVAVATGGTVVVAQYKGSGNAMMVSRASAATVTSVAMIALVIALLIIGLHQPVLNL 124 Query: 135 LFGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLIMNFSYVLL 194 LFG+ASPEV+ +AR YLIGSS S+LGIA+++AVCGALRGIG+TR SL LSLIMN YVLL Sbjct: 125 LFGSASPEVLESARIYLIGSSTSFLGIAIVQAVCGALRGIGKTRASLALSLIMNLLYVLL 184 Query: 195 NFVFINGLHMGVFGMTIAINASRYLAAVCSLIYLFRLDSSLRVKIRDLLALNWTMIKRIM 254 N VFI L+MGV GMT+AIN +R+ A C+L YLF++D++L +++RDL +M+++IM Sbjct: 185 NIVFITLLNMGVMGMTLAINIARFAGAACALYYLFKVDATLHIRLRDLFHFPISMLRKIM 244 Query: 255 FIGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIPANALSLTLITVV 314 FIGLPFAAEQMFFNGGK+LTQ+FIVSLGTYAIATNAI SSLA V QIPA+ALSLT++TVV Sbjct: 245 FIGLPFAAEQMFFNGGKLLTQVFIVSLGTYAIATNAIGSSLAAVFQIPASALSLTIVTVV 304 Query: 315 GQCMGSRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFHPPEEIVRDIFIVFL 374 GQC+G N DARKF+K P++ PLV LF+PP EIV D+F+V L Sbjct: 305 GQCIGRGNIADARKFIKSFLWLGAISLALMGLILMPLYRPLVGLFNPPPEIVNDLFVVLL 364 Query: 375 INSIAQIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGYILGIVLNMGIIGVW 434 IN++ Q+PLWS+SFI PSALRAAGDS+FTS+ SML+MWLFRV+LGYI+GIVL GI+GVW Sbjct: 365 INTLVQVPLWSISFILPSALRAAGDSRFTSITSMLTMWLFRVILGYIMGIVLGWGILGVW 424 Query: 435 LAMNCEWGVRGFIFLRRFLGEKWVQHKVI 463 LAMNCEWGVRG +FL RF G+KW HK+I Sbjct: 425 LAMNCEWGVRGAVFLWRFRGKKWYAHKLI 453 >C1PA22.1 C1PA22_BACCO MATE efflux family protein Length = 464 Score = 454 bits (1169), Expect = e-126, Method: Compositional matrix adjust. Identities = 227/459 (49%), Positives = 305/459 (66%), Gaps = 1/459 (0%) Query: 6 VNVKQGRLF-LDKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXX 64 V +KQ F L++Y +G+SIDYR++I + +PILVDQ F++ ++ +NT Sbjct: 6 VKLKQKSTFYLNRYLTGDSIDYREVIGIIIPILVDQTFLILMSFLNTAMISSAGVSAVSA 65 Query: 65 XNMIDSLNIFLISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFG 124 NM+DSLNIFL++VFIA++TGGTV+VAQYKG GN+ M + + + +F Sbjct: 66 VNMVDSLNIFLVNVFIAIATGGTVIVAQYKGIGNKEMVSKTATQAISAVAIFSVVLAVFV 125 Query: 125 IVFHGPLLHLLFGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLS 184 IVF+ P L+LLFG A V NAR YLIGS LSY IA+ +AV G LRG+G T+ L LS Sbjct: 126 IVFNTPTLNLLFGKADAAVFHNARIYLIGSCLSYPFIAIFQAVLGGLRGVGETKPCLGLS 185 Query: 185 LIMNFSYVLLNFVFINGLHMGVFGMTIAINASRYLAAVCSLIYLFRLDSSLRVKIRDLLA 244 L MN + +LN VFI HMGV G+ +I +R + S+ Y+ + + ++R KI++ L Sbjct: 186 LFMNLTNTVLNVVFITIFHMGVVGLVTSILCARIFGMIASIFYVMKYNETIRFKIKNALH 245 Query: 245 LNWTMIKRIMFIGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIPAN 304 L+++++K++MFIG+PFAAEQ+FFNGGK+LTQ FIV LGT A+ NAI+SS+ + QI N Sbjct: 246 LDFSLLKKVMFIGIPFAAEQIFFNGGKLLTQTFIVQLGTLALTENAIASSITLLFQIGPN 305 Query: 305 ALSLTLITVVGQCMGSRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFHPPEE 364 ALS+ ++T+VGQCMG RN DARKF K P F ++ LF+PP+ Sbjct: 306 ALSIAIVTIVGQCMGRRNIDDARKFAKSFIVLSSLLYIVTTAVLLPFFPVMMQLFNPPDS 365 Query: 365 IVRDIFIVFLINSIAQIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGYILGI 424 I+ IF + LI++ Q LW SFI P+ LRAAGDS FTSV S+LSMWL RV+LGYILGI Sbjct: 366 IIPTIFTIVLISAFGQPLLWPQSFIMPAVLRAAGDSTFTSVASLLSMWLLRVILGYILGI 425 Query: 425 VLNMGIIGVWLAMNCEWGVRGFIFLRRFLGEKWVQHKVI 463 GIIGVW+AM EWGVRG IF+ RF G KW HK++ Sbjct: 426 TFGFGIIGVWVAMIAEWGVRGLIFMNRFKGAKWYAHKLV 464 >C2BXR7.1 C2BXR7_LISGR MATE efflux family protein Length = 457 Score = 390 bits (1002), Expect = e-106, Method: Compositional matrix adjust. Identities = 202/448 (45%), Positives = 280/448 (62%) Query: 16 DKYFSGESIDYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXNMIDSLNIFL 75 + S E ++++ L +PI++DQ FV+G+ ++NT NM++SLNIF Sbjct: 10 KNFISSELFSSKEVVQLTIPIVIDQGFVIGMALLNTAMISSAGIAAVSAVNMVESLNIFF 69 Query: 76 ISVFIAVSTGGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIVFHGPLLHLL 135 ISVFIA+STGGTVVVAQ +G +EL+ +A + + ++FH LL L Sbjct: 70 ISVFIALSTGGTVVVAQARGKRDELLAERSAVATVVAVFGIAIVLAIIVLIFHRALLEGL 129 Query: 136 FGAASPEVMSNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLIMNFSYVLLN 195 FG +S VM NA YLIGS LSY +A++E+ CG LRG+ TR SL LS + N SYV LN Sbjct: 130 FGGSSEAVMKNASVYLIGSMLSYPALAIVESACGVLRGVADTRTSLFLSALTNLSYVALN 189 Query: 196 FVFINGLHMGVFGMTIAINASRYLAAVCSLIYLFRLDSSLRVKIRDLLALNWTMIKRIMF 255 +FI H+G+ GM IA+N +R L S+ YL + S+ LL N+ ++K++M Sbjct: 190 LLFIQVFHLGIVGMIIALNIARLLGGCVSIWYLTKRTQSIHFSFSRLLPFNFDIMKKVMS 249 Query: 256 IGLPFAAEQMFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIPANALSLTLITVVG 315 IG PFAAEQ+FFNGGKILTQIFIV LGT A+ NAI +SL +++I +L L L+T+VG Sbjct: 250 IGFPFAAEQLFFNGGKILTQIFIVQLGTLALTANAIGNSLTMLLEIIPASLGLALVTIVG 309 Query: 316 QCMGSRNTKDARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFHPPEEIVRDIFIVFLI 375 Q +G+ +TK RKF V+ L+ LFH P + + +F++ L+ Sbjct: 310 QSIGAGDTKSVRKFWLSFLALSSITTLLTSVILITVYPWLIDLFHVPASVEKQVFVLLLM 369 Query: 376 NSIAQIPLWSVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGYILGIVLNMGIIGVWL 435 S+A+I W +SFITPSALRAAGD+ FTSVVS+++MW R+VLGYILGI L G++GVW Sbjct: 370 VSVARIIAWPLSFITPSALRAAGDATFTSVVSLITMWSIRIVLGYILGITLGYGLLGVWA 429 Query: 436 AMNCEWGVRGFIFLRRFLGEKWVQHKVI 463 AM EW VRG IF R KW++ +++ Sbjct: 430 AMCFEWFVRGIIFAVRLYRGKWMRKRLV 457 >C3RHV7.1 C3RHV7_9MOLU MATE efflux family protein Length = 442 Score = 255 bits (652), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 145/439 (33%), Positives = 238/439 (54%) Query: 25 DYRQIISLFLPILVDQAFVVGLNVVNTXXXXXXXXXXXXXXNMIDSLNIFLISVFIAVST 84 D + + L +P++V+Q V+ + +T +++D++NI LI++F A+ T Sbjct: 4 DNKALKKLIIPLVVEQLLVITVGFADTVMVASVGEAAVSAVSLVDAINILLINIFAALGT 63 Query: 85 GGTVVVAQYKGSGNELMXXXXXXXXXXXXXLMAFAIGLFGIVFHGPLLHLLFGAASPEVM 144 GG VV AQ+ G G++ L++ I + I F+ PLLH++FG +VM Sbjct: 64 GGAVVAAQFIGQGSKDKAKYSAKQLILITALLSVIIMVIVIAFNVPLLHMVFGNVEVDVM 123 Query: 145 SNARTYLIGSSLSYLGIAVMEAVCGALRGIGRTRVSLVLSLIMNFSYVLLNFVFINGLHM 204 NA Y + S++SY IA+ + R IG +++S++ S +MN ++LN +FI Sbjct: 124 KNAEIYFLFSAMSYPFIALYNSGAALFRAIGNSKISMINSAVMNVINIILNAIFIFVFKW 183 Query: 205 GVFGMTIAINASRYLAAVCSLIYLFRLDSSLRVKIRDLLALNWTMIKRIMFIGLPFAAEQ 264 GVFG +A +R +A + L L D+ + V ++ IK+I+ IG+P E Sbjct: 184 GVFGAVLATLIARAVACIVILKMLSHRDNDVCVNDYLHWKFDFMYIKKILAIGIPSGLEN 243 Query: 265 MFFNGGKILTQIFIVSLGTYAIATNAISSSLAGVMQIPANALSLTLITVVGQCMGSRNTK 324 F GKIL Q I + GTY+IA NA+S++LA + IP A+SL ++TVVGQC+G+ + K Sbjct: 244 GMFQLGKILVQSLIATFGTYSIAANAVSNNLAQMQIIPGMAMSLAMVTVVGQCVGANDYK 303 Query: 325 DARKFVKXXXXXXXXXXXXXXXXXXPVFNPLVSLFHPPEEIVRDIFIVFLINSIAQIPLW 384 A+ +VK +++ + +E + +++I +W Sbjct: 304 QAKYYVKKLLKITYLSMAGLIVVLIIATPSILTFYSLSKETTDLAYQCIFLHAIIAALIW 363 Query: 385 SVSFITPSALRAAGDSKFTSVVSMLSMWLFRVVLGYILGIVLNMGIIGVWLAMNCEWGVR 444 SF P+ALRAA D+KFT +VS SM +FR+ Y+LG +N+G+IGVW+AM +WGVR Sbjct: 364 PTSFTFPNALRAANDAKFTMIVSAASMLIFRLCFSYVLGQYMNLGLIGVWIAMFIDWGVR 423 Query: 445 GFIFLRRFLGEKWVQHKVI 463 F+ R+ KW+ +++ Sbjct: 424 CIFFIWRYFSGKWMNRQLV 442 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.143 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,265,457,996 Number of extensions: 80043559 Number of successful extensions: 257975 Number of sequences better than 1.0e-05: 2123 Number of HSP's gapped: 255840 Number of HSP's successfully gapped: 2136 Length of query: 463 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 324 Effective length of database: 1,751,175,118 Effective search space: 567380738232 Effective search space used: 567380738232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 133 (55.8 bits)