BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0007.p (296 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3A7.1 B3K3A7_9BACI Binding-protein-dependent transport system... 551 e-155 B1D7W4.1 B1D7W4_9BACL Binding-protein-dependent transport system... 416 e-114 B1DAC3.1 B1DAC3_9BACL Binding-protein-dependent transport system... 252 4e-65 B3KA94.1 B3KA94_9BACI Binding-protein-dependent transport system... 249 2e-64 B1DBH8.1 B1DBH8_9BACL Binding-protein-dependent transport system... 249 3e-64 >B3K3A7.1 B3K3A7_9BACI Binding-protein-dependent transport systems inner membrane component Length = 293 Score = 551 bits (1419), Expect = e-155, Method: Compositional matrix adjust. Identities = 268/293 (91%), Positives = 285/293 (97%) Query: 4 VKTSWMKRQQHLGYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTKFVGLDNIQR 63 +KTSWMKRQQ+LGYLFIGPNM+GV+LFFI+PA+YSFYLMFTDYKFMS DTKFVGL NIQ+ Sbjct: 1 MKTSWMKRQQYLGYLFIGPNMVGVMLFFIVPALYSFYLMFTDYKFMSSDTKFVGLANIQK 60 Query: 64 MLGDEVFYIAIKNTLMFLLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAV 123 MLGDEVFYI+IKNTL FLLSVP+SIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAV Sbjct: 61 MLGDEVFYISIKNTLFFLLSVPISIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAV 120 Query: 124 AFVWMLLFQPNNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAA 183 AFVWMLLF PNNGPINGIL++ GI NPPGWLSTM+SSMYAIDIIWIWFMLGYNMIIYLAA Sbjct: 121 AFVWMLLFHPNNGPINGILKSFGITNPPGWLSTMDSSMYAIDIIWIWFMLGYNMIIYLAA 180 Query: 184 LQEVSGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGG 243 LQEVS ELLEAAKIDGAR WQTVRSILWPLVSPTTFLLLITGLIM+IKQFGIIQAITQGG Sbjct: 181 LQEVSTELLEAAKIDGARAWQTVRSILWPLVSPTTFLLLITGLIMTIKQFGIIQAITQGG 240 Query: 244 PGNSTTVLSLFIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 PGNSTTVLSLFIYQNAFRYYEMGYASA+SWALF+IIMIFTV+QW+GQKRWVHY Sbjct: 241 PGNSTTVLSLFIYQNAFRYYEMGYASAISWALFLIIMIFTVIQWIGQKRWVHY 293 >B1D7W4.1 B1D7W4_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 416 bits (1068), Expect = e-114, Method: Compositional matrix adjust. Identities = 194/292 (66%), Positives = 246/292 (84%) Query: 5 KTSWMKRQQHLGYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTKFVGLDNIQRM 64 + SW+++Q +GY F+ PNM+G++ FFI+PA+YSF +MFTDY+F +P+ KF G+DNI R+ Sbjct: 3 RKSWIQKQGWIGYAFVLPNMLGIVAFFILPALYSFVMMFTDYQFANPNWKFTGMDNINRL 62 Query: 65 LGDEVFYIAIKNTLMFLLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVA 124 GD+ FY ++K+T +FL SVPVS+GLAF+VA+VLNRSVYLK LLR ++F+PYI+SGVAVA Sbjct: 63 FGDDQFYASLKHTAIFLFSVPVSLGLAFLVALVLNRSVYLKGLLRGMFFLPYISSGVAVA 122 Query: 125 FVWMLLFQPNNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAAL 184 FVWMLLFQP+ GPIN +LR IGI +PPGW + +S+MYAID+I IWFMLGYNMIIYLAAL Sbjct: 123 FVWMLLFQPSQGPINEVLRFIGIVSPPGWFADTKSAMYAIDVIQIWFMLGYNMIIYLAAL 182 Query: 185 QEVSGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGGP 244 QE+S E LEAA+IDGA + I+WPLVSPTTF+LLITGLIM++K F +IQAIT GGP Sbjct: 183 QEISTEQLEAARIDGAGSATITWRIVWPLVSPTTFMLLITGLIMTVKSFSLIQAITGGGP 242 Query: 245 GNSTTVLSLFIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 STT+LSLF+Y+ AF YYEMGYAS +SW LFV I+I T++QW+GQKRWVHY Sbjct: 243 SGSTTILSLFVYKTAFSYYEMGYASTISWFLFVFILIITIIQWVGQKRWVHY 294 >B1DAC3.1 B1DAC3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 252 bits (643), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 127/289 (43%), Positives = 191/289 (66%), Gaps = 2/289 (0%) Query: 9 MKRQQHLGYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTKFVGLDNIQRMLGDE 68 ++R + GY F+ PN++G ++F P SF + FT++ + KF G N R+ D+ Sbjct: 6 LRRNRLTGYTFLLPNILGFLIFICFPVCASFLISFTEWNGFG-EIKFTGFSNYARLWSDD 64 Query: 69 VFYIAIKNTL-MFLLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVAFVW 127 F I++ N+L M +SVPV++ LA + AV LNR + KL R F+P+IT+ +AVA VW Sbjct: 65 TFRISLINSLVMTAVSVPVTLMLAILAAVALNRGIKGVKLFRTAIFLPHITATIAVAVVW 124 Query: 128 MLLFQPNNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAALQEV 187 LL+ P GPING LR++GIA+PP WL++ + ++ ++ I+ IW +GY M++YLA LQ + Sbjct: 125 QLLYNPTMGPINGFLRSLGIAHPPAWLASTQWALISVIIVSIWHSIGYYMVLYLAGLQGI 184 Query: 188 SGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGGPGNS 247 +L EAA+IDGA R+I P++SP F +I G+I S K F ++ +T+GGPG S Sbjct: 185 PKDLYEAAEIDGAGKVSQFRNITLPMLSPVIFFTVIMGIINSFKVFDLVYVLTKGGPGRS 244 Query: 248 TTVLSLFIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 T +L IY AF+ YE GYASA+++ LF II++ T++Q+ GQKRWV+Y Sbjct: 245 THMLVYDIYDTAFKRYEYGYASAMAYVLFAIILVITLIQFRGQKRWVNY 293 >B3KA94.1 B3KA94_9BACI Binding-protein-dependent transport systems inner membrane component Length = 315 Score = 249 bits (637), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 125/298 (41%), Positives = 199/298 (66%), Gaps = 3/298 (1%) Query: 2 NDVKTSWMKRQQHL-GYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMS--PDTKFVGL 58 D ++R ++L G F+ P +IGVI+ + P + + L F D+ F+ ++VG Sbjct: 18 EDKSRGSLQRGENLWGIAFVSPMLIGVIILVLFPILATLVLGFADWNFVQGWDGIQWVGF 77 Query: 59 DNIQRMLGDEVFYIAIKNTLMFLLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYIT 118 N +++L D++F +++N ++FLL+VPV + ++ +A++++R VY+K + YFMPYI+ Sbjct: 78 QNFRQLLEDDMFIKSVRNNILFLLTVPVYMMISMTLAILIDRFVYMKGYFKVAYFMPYIS 137 Query: 119 SGVAVAFVWMLLFQPNNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMI 178 + VAVA VW +LFQP+ GPIN ILR +G++NPP W++ ++ +I +I IW +G+N+I Sbjct: 138 NIVAVAVVWQVLFQPSYGPINEILRTLGMSNPPKWIADPNFALISIMLISIWISIGFNLI 197 Query: 179 IYLAALQEVSGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQA 238 IY+A LQ + +L EAA IDGA W R I PL+SPT+F LL+TG+I + K F II Sbjct: 198 IYIAGLQSIPKDLYEAADIDGANGWTKFRRITLPLLSPTSFFLLVTGIISTFKVFDIIAV 257 Query: 239 ITQGGPGNSTTVLSLFIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 +TQGGP STT++ ++Y AF ++GYAS+++ LF +M+ T+ QW QK+WV+Y Sbjct: 258 MTQGGPIGSTTMMVWYLYDTAFVNLKVGYASSIAAVLFGFVMLITLGQWAAQKKWVNY 315 >B1DBH8.1 B1DBH8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 310 Score = 249 bits (635), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 120/283 (42%), Positives = 190/283 (67%), Gaps = 2/283 (0%) Query: 16 GYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMS--PDTKFVGLDNIQRMLGDEVFYIA 73 G+LF+ P +IGV + ++P + + L F D+ F+ +VG N +++ D+ F + Sbjct: 28 GWLFVSPMLIGVCILVLLPIVATIVLSFADWNFVQGWKGLDWVGFGNFKKLFHDDQFIRS 87 Query: 74 IKNTLMFLLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVAFVWMLLFQP 133 ++N +FLL+VP+ + ++ ++AV+++R VYLK + YFMPYI+S VAVA VW +LFQP Sbjct: 88 VRNNFIFLLAVPIYMLISMVLAVIIDRHVYLKSYFKVAYFMPYISSIVAVAIVWQVLFQP 147 Query: 134 NNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAALQEVSGELLE 193 + GPIN IL G++NPP W++ ++ +I +I +W +G+NMIIY+A LQ + +L E Sbjct: 148 SYGPINQILHTFGVSNPPKWIADPHFALASIMMIVVWTSIGFNMIIYIAGLQSIPKDLYE 207 Query: 194 AAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGGPGNSTTVLSL 253 AA IDGA W I +PL+SPT+F LL+TG+I + K F II +TQGGP ST+++ Sbjct: 208 AADIDGANGWTKFARITFPLLSPTSFFLLVTGIISTFKVFDIIAVMTQGGPIGSTSLMVW 267 Query: 254 FIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 ++Y AF ++GYAS+++ LF+ +++ T QWL QK+WV+Y Sbjct: 268 YLYDTAFVNLKVGYASSIAAMLFIFVLLITFGQWLAQKKWVNY 310 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.143 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,795,838,380 Number of extensions: 70960393 Number of successful extensions: 303755 Number of sequences better than 1.0e-05: 6420 Number of HSP's gapped: 293601 Number of HSP's successfully gapped: 6431 Length of query: 296 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 161 Effective length of database: 1,788,859,178 Effective search space: 288006327658 Effective search space used: 288006327658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)