BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0040.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9VNJ6.1 A9VNJ6_BACWK Major facilitator superfamily MFS_1 191 6e-47 C2SI95.1 C2SI95_BACCE Permease 191 6e-47 C2PU83.1 C2PU83_BACCE Permease 191 7e-47 C3A462.1 C3A462_BACMY Permease 191 8e-47 C2YPV4.1 C2YPV4_BACCE Permease 189 2e-46 >A9VNJ6.1 A9VNJ6_BACWK Major facilitator superfamily MFS_1 Length = 422 Score = 191 bits (486), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 103/296 (34%), Positives = 171/296 (57%), Gaps = 18/296 (6%) Query: 12 SNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFAGV 71 + RN++L GK+ S+LG+G+YTF +GL++LK TGSG FA T++CG +PR++ P AG Sbjct: 16 ATRNIILMMIGKMTSLLGAGIYTFAMGLYVLKTTGSGMGFATTLICGSIPRMICGPIAGA 75 Query: 72 TADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVSSS 131 ADR+NRR L+I +D SLL +Y S LLS+C++FY ++++SS Sbjct: 76 VADRVNRRWLVIGTDLLSSLTMLIMFILATIFGPSLLFIYVSAALLSICASFYSVSLTSS 135 Query: 132 LLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLMSC 191 + LV++E+IQ+A +LNQ A S+ N+LAP++GG+++ +K F LLN+ F ++ ++ Sbjct: 136 IPNLVDEERIQKASALNQTASSLSNILAPIIGGVVFGFFSIKTFFLLNSITFFLAVIVQL 195 Query: 192 GLRFKPSTVTGVQTELPDSVPGGDARRVQTKVLAELRSSLAGGISYVFKKPVIRAXXXXX 251 + F + EL +S +S+ G SYV ++ I Sbjct: 196 FIVFDLY-----KKELAESKE-------------HFLTSIKEGFSYVKRQHEIYGLMKIA 237 Query: 252 XXXXXXXXSLNVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLLTVRRQKTSP 307 +L V +P++ +K+L LSS Q G + M+A G+L +++L+VR++ +P Sbjct: 238 VGVNFFASALFVSLPYIIIKNLHLSSKQLGVVEGMLAVGMLIGAIVLSVRKEVNNP 293 >C2SI95.1 C2SI95_BACCE Permease Length = 422 Score = 191 bits (486), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 103/296 (34%), Positives = 171/296 (57%), Gaps = 18/296 (6%) Query: 12 SNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFAGV 71 + RN++L GK+ S+LG+G+YTF +GL++LK TGSG FA T++CG +PR++ P AG Sbjct: 16 ATRNIILMMIGKMTSLLGAGIYTFAMGLYVLKTTGSGMGFATTLICGSIPRMICGPIAGA 75 Query: 72 TADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVSSS 131 ADR+NRR L+I +D SLL +Y S LLS+C++FY ++++SS Sbjct: 76 VADRVNRRWLVIGTDLLSSLTMLIMFILATIFGPSLLFIYVSAALLSICASFYSVSLTSS 135 Query: 132 LLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLMSC 191 + LV++E+IQ+A +LNQ A S+ N+LAP++GG+++ +K F LLN+ F ++ ++ Sbjct: 136 IPNLVDEERIQKASALNQTASSLSNILAPIIGGVVFGFFSIKTFFLLNSITFFLAVIVQL 195 Query: 192 GLRFKPSTVTGVQTELPDSVPGGDARRVQTKVLAELRSSLAGGISYVFKKPVIRAXXXXX 251 + F + EL +S +S+ G SYV ++ I Sbjct: 196 FIVFDLY-----KKELAESKE-------------HFLTSIKEGFSYVKRQHEIYGLMKIA 237 Query: 252 XXXXXXXXSLNVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLLTVRRQKTSP 307 +L V +P++ +K+L LSS Q G + M+A G+L +++L+VR++ +P Sbjct: 238 VGVNFFASALFVSLPYIIIKNLHLSSKQLGVVEGMLAVGMLIGAIVLSVRKEVNNP 293 >C2PU83.1 C2PU83_BACCE Permease Length = 422 Score = 191 bits (485), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 103/296 (34%), Positives = 171/296 (57%), Gaps = 18/296 (6%) Query: 12 SNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFAGV 71 + RN++L GK+ S+LG+G+YTF +GL++LK TGSG FA T++CG +PR++ P AG Sbjct: 16 ATRNIILMMIGKMTSLLGAGIYTFAMGLYVLKTTGSGMGFATTLICGSIPRMICGPIAGA 75 Query: 72 TADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVSSS 131 ADR+NRR L+I +D SLL +Y S LLS+C++FY ++++SS Sbjct: 76 VADRVNRRWLVIGTDLLSSLTMLIMFILATIFGPSLLFIYVSAALLSICASFYSVSLTSS 135 Query: 132 LLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLMSC 191 + LV++E+IQ+A +LNQ A S+ N+LAP++GG+++ +K F LLN+ F ++ ++ Sbjct: 136 IPNLVDEERIQKASALNQTASSLSNILAPIIGGVVFGFFSIKTFFLLNSITFFLAVIVQL 195 Query: 192 GLRFKPSTVTGVQTELPDSVPGGDARRVQTKVLAELRSSLAGGISYVFKKPVIRAXXXXX 251 + F + EL +S +S+ G SYV ++ I Sbjct: 196 FIVFDLY-----KKELAESKE-------------HFLTSIKEGFSYVKRQHEIYGLMKIA 237 Query: 252 XXXXXXXXSLNVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLLTVRRQKTSP 307 +L V +P++ +K+L LSS Q G + M+A G+L +++L+VR++ +P Sbjct: 238 VGVNFFASALFVSLPYIIIKNLHLSSKQLGVVEGMLAVGMLIGAIVLSVRKEVNNP 293 >C3A462.1 C3A462_BACMY Permease Length = 422 Score = 191 bits (485), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 104/296 (35%), Positives = 173/296 (58%), Gaps = 18/296 (6%) Query: 12 SNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFAGV 71 + RN++L GK+ S+LG+G+YTF +GL++LK TGSG FA T++CG +PR++ P AG Sbjct: 16 ATRNIILMMIGKMTSLLGAGIYTFAMGLYVLKTTGSGMGFATTLICGSIPRMICGPIAGA 75 Query: 72 TADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVSSS 131 ADR+NRR L+I +D SLL +Y S LLS+C++FY ++++SS Sbjct: 76 VADRVNRRWLVIGTDLLSSLTMLIMFILATIFGPSLLFIYVSAALLSICASFYSVSLTSS 135 Query: 132 LLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLMSC 191 + LV++E+IQ+A +LNQ A S+ N+LAP++GG+++ +K F LLN+ F ++ ++ Sbjct: 136 IPNLVDEERIQKASALNQTASSLSNILAPIIGGVVFGFFSIKTFFLLNSITFFLAVIVQL 195 Query: 192 GLRFKPSTVTGVQTELPDSVPGGDARRVQTKVLAELRSSLAGGISYVFKKPVIRAXXXXX 251 + F + EL V++K +S+ G SYV ++ I Sbjct: 196 FIVFDLY-----KKEL-----------VESK--EHFLTSIKEGFSYVKRQHEIYGLMKIA 237 Query: 252 XXXXXXXXSLNVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLLTVRRQKTSP 307 +L V +P++ +K+L LSS Q G + M+A G+L +++L+VR++ +P Sbjct: 238 VGVNFFASALFVSLPYIIIKNLHLSSKQLGVVEGMLAVGMLIGAIVLSVRKEVNNP 293 >C2YPV4.1 C2YPV4_BACCE Permease Length = 422 Score = 189 bits (481), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 104/300 (34%), Positives = 168/300 (56%), Gaps = 18/300 (6%) Query: 12 SNRNMLLFFTGKLASVLGSGMYTFVVGLFILKLTGSGGSFAVTMVCGLLPRILLAPFAGV 71 + RN++L GK+ S+LG+G+YTF +GL++LK TGSG FA T+VCG LPR++ P AG Sbjct: 16 ATRNIILMMIGKMTSLLGAGIYTFAMGLYVLKTTGSGMGFATTLVCGSLPRMICGPIAGA 75 Query: 72 TADRMNRRKLLICSDXXXXXXXXXXXXXXXXXXXSLLPVYASLVLLSVCSTFYGIAVSSS 131 ADR+NR+ L+I +D SLL +Y S LLS+C++FY +A++SS Sbjct: 76 VADRVNRKWLVIGTDLLSSLTMLIMFILATIFGPSLLFIYVSAALLSICASFYSVALTSS 135 Query: 132 LLQLVEDEQIQRAGSLNQIAGSIGNLLAPVLGGMLYALVPLKLFMLLNAAGFAVSTLMSC 191 + LV +E+IQ+A +LNQ A S+ N+L P++GG+++ +K F LLN+ F ++ ++ Sbjct: 136 IPSLVNEERIQKASALNQTAASLSNILGPIIGGVVFGFFSIKSFFLLNSITFFLAVILQL 195 Query: 192 GLRFKPSTVTGVQTELPDSVPGGDARRVQTKVLAELRSSLAGGISYVFKKPVIRAXXXXX 251 + F V+++ +S+ G SYV ++ I Sbjct: 196 FIVFDLYKKEMVESK------------------EHFLTSIKEGFSYVKRQHEIYGLMKIA 237 Query: 252 XXXXXXXXSLNVVMPFVAVKSLSLSSSQYGTINAMMAAGILAMSLLLTVRRQKTSPAGSL 311 L V +P++ V +L LSS Q GT+ M+A G+L ++ L+VR++ +P S+ Sbjct: 238 LWVNFFACGLTVALPYIIVHTLHLSSRQLGTVEGMLAVGMLMGAIALSVRKEVNNPFRSI 297 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,476,028,085 Number of extensions: 50179122 Number of successful extensions: 210898 Number of sequences better than 1.0e-05: 1439 Number of HSP's gapped: 210445 Number of HSP's successfully gapped: 1439 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)