BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0247.0037.p
         (138 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B4BKQ7.1 B4BKQ7_9BACI Large conductance mechanosensitive channel...   142   9e-33
B7GLN3.1 MSCL_ANOFW Large-conductance mechanosensitive channel        141   2e-32
A4IMP7.1 MSCL_GEOTN Large-conductance mechanosensitive channel        141   2e-32
A7GTU7.1 MSCL_BACCN Large-conductance mechanosensitive channel        140   3e-32
B3K5L7.1 B3K5L7_9BACI Large conductance mechanosensitive channel...   139   8e-32

>B4BKQ7.1 B4BKQ7_9BACI Large conductance mechanosensitive channel protein
          Length = 149

 Score =  142 bits (358), Expect = 9e-33,   Method: Compositional matrix adjust.
 Identities = 74/131 (56%), Positives = 90/131 (68%), Gaps = 1/131 (0%)

Query: 4   IEMWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLKGLSFA 63
           I MW EFKKFA++GNV+DLAV V+IG AFGKIVSSLV DIIMPLVG+L GGID   LS+ 
Sbjct: 17  IRMWNEFKKFAIRGNVIDLAVGVIIGGAFGKIVSSLVNDIIMPLVGLLLGGIDFSNLSWK 76

Query: 64  YGDAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNR-FKRXXXXXXXXXXXXXXXXXXXXXX 122
            G AVVKYG F+Q+VVDF IIAFSIF+ VKL+N+ ++R                      
Sbjct: 77  VGKAVVKYGAFIQTVVDFLIIAFSIFLFVKLINKLYERVKKQEEMEETEPTLTKEEELLT 136

Query: 123 XIRDLLRTDKQ 133
            IRDLL+  ++
Sbjct: 137 EIRDLLKQQRE 147


>B7GLN3.1 MSCL_ANOFW Large-conductance mechanosensitive channel
          Length = 131

 Score =  141 bits (355), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 73/129 (56%), Positives = 89/129 (68%), Gaps = 1/129 (0%)

Query: 6   MWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLKGLSFAYG 65
           MW+EFKKFA++GNV+DLAV V+IG AFGKIVSSLV DIIMPLVG++ GGID  GLS+  G
Sbjct: 1   MWQEFKKFAVRGNVIDLAVGVIIGGAFGKIVSSLVNDIIMPLVGLILGGIDFSGLSWKVG 60

Query: 66  DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNRF-KRXXXXXXXXXXXXXXXXXXXXXXXI 124
           +A VKYG FLQ+VVDF +IAFSIF+ VKL+N   +R                       I
Sbjct: 61  EAEVKYGAFLQTVVDFLVIAFSIFLFVKLLNNLHERIKKQEETKQTAPTMTKEQQLLTEI 120

Query: 125 RDLLRTDKQ 133
           RDLL+  K+
Sbjct: 121 RDLLKQQKE 129


>A4IMP7.1 MSCL_GEOTN Large-conductance mechanosensitive channel
          Length = 131

 Score =  141 bits (355), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 73/129 (56%), Positives = 89/129 (68%), Gaps = 1/129 (0%)

Query: 6   MWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLKGLSFAYG 65
           MW EFKKFA++GNV+DLAV V+IG AFGKIVSSLV DIIMPLVG+L GGID   LS+  G
Sbjct: 1   MWNEFKKFAIRGNVIDLAVGVIIGGAFGKIVSSLVNDIIMPLVGLLLGGIDFSNLSWKVG 60

Query: 66  DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNR-FKRXXXXXXXXXXXXXXXXXXXXXXXI 124
            AVVKYG F+Q+VVDF IIAFSIF+ VKL+N+ ++R                       I
Sbjct: 61  KAVVKYGAFIQTVVDFLIIAFSIFLFVKLINKLYERVKKQEEMEETEPTLTKEEELLTEI 120

Query: 125 RDLLRTDKQ 133
           RDLL+  ++
Sbjct: 121 RDLLKQQRE 129


>A7GTU7.1 MSCL_BACCN Large-conductance mechanosensitive channel
          Length = 132

 Score =  140 bits (354), Expect = 3e-32,   Method: Compositional matrix adjust.
 Identities = 68/92 (73%), Positives = 80/92 (86%)

Query: 6  MWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLKGLSFAYG 65
          MW EFKKFALKGNV+DLAV VVIG AFGKIVSSLV+D+IMPLVG+L GG++  GLSF +G
Sbjct: 1  MWNEFKKFALKGNVMDLAVGVVIGGAFGKIVSSLVSDVIMPLVGLLLGGVNFTGLSFTFG 60

Query: 66 DAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNR 97
           AVVKYG F+Q+VVDF IIAFSIF+ +KL N+
Sbjct: 61 KAVVKYGAFIQTVVDFLIIAFSIFLFIKLFNK 92


>B3K5L7.1 B3K5L7_9BACI Large conductance mechanosensitive channel protein
          Length = 132

 Score =  139 bits (350), Expect = 8e-32,   Method: Compositional matrix adjust.
 Identities = 71/133 (53%), Positives = 90/133 (67%), Gaps = 2/133 (1%)

Query: 4   IEMWEEFKKFALKGNVLDLAVAVVIGAAFGKIVSSLVTDIIMPLVGILTGGIDLKGLSFA 63
           + + +EFK FALKGNVLDLA+ V+IGAAFGKIVSSLV+DIIMP++G+L GG+DL GL   
Sbjct: 1   MNLLKEFKTFALKGNVLDLAIGVIIGAAFGKIVSSLVSDIIMPVIGLLLGGVDLSGLKAT 60

Query: 64  YGDAVVKYGVFLQSVVDFFIIAFSIFMVVKLVNRFKRXXXXXXXXXXXXXXXXXXXXXXX 123
            GDA + YGVFLQ+VVDF I++FSIFM ++ +NRFKR                       
Sbjct: 61  IGDATLTYGVFLQTVVDFLIVSFSIFMFIRTLNRFKR--KEETKAEEPPAPSKEEVLLAE 118

Query: 124 IRDLLRTDKQGQK 136
           IRDLL+   Q  +
Sbjct: 119 IRDLLKQQNQASE 131


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.328    0.146    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 620,736,630
Number of extensions: 20668948
Number of successful extensions: 90442
Number of sequences better than 1.0e-05: 1065
Number of HSP's gapped: 89661
Number of HSP's successfully gapped: 1065
Length of query: 138
Length of database: 3,060,696,203
Length adjustment: 102
Effective length of query: 36
Effective length of database: 2,099,752,673
Effective search space: 75591096228
Effective search space used: 75591096228
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 125 (52.8 bits)