BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0036.p (366 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL55.1 B3KL55_9BACI Riboflavin biosynthesis protein RibD EC=1.... 505 e-141 B1DJB0.1 B1DJB0_9BACL Riboflavin biosynthesis protein RibD EC=1.... 447 e-123 Q9KCL7.1 Q9KCL7_BACHD Riboflavin specific deaminase(Diaminohydro... 355 4e-96 Q8CXN3.1 Q8CXN3_OCEIH Riboflavin specific deaminase (Diaminohydr... 332 3e-89 Q65HW4.1 Q65HW4_BACLD Riboflavin-specific deaminase 330 9e-89 >B3KL55.1 B3KL55_9BACI Riboflavin biosynthesis protein RibD EC=1.1.1.193 EC=3.5.4.26 Length = 369 Score = 505 bits (1301), Expect = e-141, Method: Compositional matrix adjust. Identities = 247/365 (67%), Positives = 288/365 (78%), Gaps = 1/365 (0%) Query: 1 MDTMNDEFYMSLALDMAERAQGQTGINPVVGCVVVKDGAMIGLGTHLQRGTGHAEVHALN 60 MD +NDEFYMSLALD+AERAQGQTGINPVVGCVVVKDGA+ G+GTHL+RGT HAEVHALN Sbjct: 4 MDIVNDEFYMSLALDLAERAQGQTGINPVVGCVVVKDGALAGVGTHLERGTPHAEVHALN 63 Query: 61 MAAGKAQGSTAYVTLEPCSHYGKTPPCSQRLIDEGVARVVVACEDPNPQVAGRGITMLRE 120 MA KA GST YVTLEPCSHYG TPPCS+RLI EGV RVVVACEDPNP VAGRGI MLR Sbjct: 64 MAGPKAAGSTVYVTLEPCSHYGATPPCSERLIAEGVRRVVVACEDPNPLVAGRGIQMLRA 123 Query: 121 QGIEVEVGLLRSRALRLNEKFIKYILTKQPFVTLKSASTLDGKLATRTGDSKWISNGQAR 180 GIEVE G+L+ RALRLNE F+K+I T +PFVTLK+ASTLDGK+A++TGDSKWISN ++R Sbjct: 124 AGIEVETGILKDRALRLNEAFVKFITTGRPFVTLKTASTLDGKIASKTGDSKWISNEKSR 183 Query: 181 EIVHTLRHRHQGIMVGVNTVIADNPSLTTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQ 240 E+VHTLRHRHQGIMVGV+TVIADNPSLTTR+ V GL+PVRI++DS+LRIPLDS VV DG Sbjct: 184 ELVHTLRHRHQGIMVGVSTVIADNPSLTTRLGVEGLHPVRIIVDSSLRIPLDSHVVSDGL 243 Query: 241 APTIVVTTESADPVKRAALLDAGVQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXX 300 APT ++ TE AD KR+ L + G Q+ G GPRVDLK A+ Sbjct: 244 APTWILATEQADDTKRSELEELGAQVFPCGPGPRVDLKVALLQLGQKGIASILLEGGGTL 303 Query: 301 NGAMLENGLVDRVVLFFAPKIVGGGSASPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCI 360 NG+MLE LVDR++ F APKI+GG A PG + F G E M A++L GLE E LGDN+ + Sbjct: 304 NGSMLEQKLVDRLIQFIAPKIIGGYDA-PGNYMFSGYEQMNQAISLTGLETERLGDNIYV 362 Query: 361 SGTPI 365 +G P+ Sbjct: 363 TGIPV 367 >B1DJB0.1 B1DJB0_9BACL Riboflavin biosynthesis protein RibD EC=1.1.1.193 EC=3.5.4.26 Length = 368 Score = 447 bits (1149), Expect = e-123, Method: Compositional matrix adjust. Identities = 213/365 (58%), Positives = 276/365 (75%), Gaps = 1/365 (0%) Query: 1 MDTMNDEFYMSLALDMAERAQGQTGINPVVGCVVVKDGAMIGLGTHLQRGTGHAEVHALN 60 M+ +ND +YMSLAL++A +A GQTGINPVVGCVVVKDG +IG+GTHL+RGTGHAEVHAL Sbjct: 1 MEILNDSYYMSLALELAAKASGQTGINPVVGCVVVKDGRIIGVGTHLKRGTGHAEVHALQ 60 Query: 61 MAAGKAQGSTAYVTLEPCSHYGKTPPCSQRLIDEGVARVVVACEDPNPQVAGRGITMLRE 120 MA +A+G+T YVTLEPCSHYGKTPPC +R+I+ ARVVVA DPNPQVAGRGI LRE Sbjct: 61 MAGDEAEGATVYVTLEPCSHYGKTPPCCERIINAKAARVVVATTDPNPQVAGRGIARLRE 120 Query: 121 QGIEVEVGLLRSRALRLNEKFIKYILTKQPFVTLKSASTLDGKLATRTGDSKWISNGQAR 180 +GIEVEVGLL ++ +NEKF KYI ++ P+VTLK+ASTLDGK+A+ TGDS+WI+ AR Sbjct: 121 EGIEVEVGLLEEQSRSMNEKFNKYITSRLPYVTLKTASTLDGKIASHTGDSRWITGSTAR 180 Query: 181 EIVHTLRHRHQGIMVGVNTVIADNPSLTTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQ 240 E VHTLRH+H+ IMVG+ T++AD+P LTTR VP ++PVRI++DS LR+PLD+ VV DG Sbjct: 181 EQVHTLRHQHEAIMVGIGTLLADDPQLTTRAAVPAIHPVRIIVDSQLRLPLDARVVTDGL 240 Query: 241 APTIVVTTESADPVKRAALLDAGVQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXX 300 A TIV+ + ADP + AL +AGV+++ G G +V L AM Sbjct: 241 ARTIVLASAKADPSREQALTEAGVEVIRCGDGEQVVLAEAMRKLGELEIGSILLEGGGKL 300 Query: 301 NGAMLENGLVDRVVLFFAPKIVGGGSASPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCI 360 NGAMLE GL+D+++++ A KI+GG SPGTF F G E M A+ L+ ++VE+ G+++CI Sbjct: 301 NGAMLEAGLIDKIMIYMAVKIIGGLD-SPGTFTFAGFEKMSEAIELDHVKVEMAGEDICI 359 Query: 361 SGTPI 365 SG P+ Sbjct: 360 SGYPV 364 >Q9KCL7.1 Q9KCL7_BACHD Riboflavin specific deaminase(Diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase) EC=3.5.4.- Length = 368 Score = 355 bits (911), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 186/357 (52%), Positives = 243/357 (68%), Gaps = 4/357 (1%) Query: 4 MNDEFYMSLALDMAERAQGQTGINPVVGCVVVKDGAMIGLGTHLQRGTGHAEVHALNMAA 63 M DE YM LAL +AE A+GQT NP+VG VVVKDG ++G+G HL+ G HAEVHA+ MA Sbjct: 1 MTDEQYMKLALALAESARGQTSPNPLVGAVVVKDGQIVGMGAHLKAGEPHAEVHAIRMAG 60 Query: 64 GKAQGSTAYVTLEPCSHYGKTPPCSQRLIDEGVARVVVACEDPNPQVAGRGITMLREQGI 123 KA+G+TAYVTLEPCSHYGKTPPC+ LI+ GVARVV+A EDP P+V+GRGI L++ GI Sbjct: 61 EKAKGATAYVTLEPCSHYGKTPPCADLLIEAGVARVVIATEDPFPKVSGRGIRKLQQAGI 120 Query: 124 EVEVGLLRSRALRLNEKFIKYILTKQPFVTLKSASTLDGKLATRTGDSKWISNGQAREIV 183 EVEVG+ + AL LN F ++ TK+P+VT+KSA+TLDGK AT G+SKWI++ QARE V Sbjct: 121 EVEVGICKQEALDLNRIFFHFVRTKRPYVTIKSATTLDGKTATVQGNSKWITSEQAREDV 180 Query: 184 HTLRHRHQGIMVGVNTVIADNPSLTTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQAPT 243 H RH H I+VGV TV+ADNPSLTTR+ GLNPVR+++D LR PLD V+ DG+APT Sbjct: 181 HRYRHEHDAILVGVGTVLADNPSLTTRLAAGGLNPVRVILDRRLRTPLDRNVITDGKAPT 240 Query: 244 IVVTTESADPVKRAALLDAGVQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGA 303 + T E AD K+ AL + GVQ+ + P V ++A + NG+ Sbjct: 241 WIFTEERADKAKKKALEEKGVQVF---SLPNVSVEAVLETLREKNILSVFVEGGATVNGS 297 Query: 304 MLENGLVDRVVLFFAPKIVGGGSASPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCI 360 ++ G VD+V+ + APK+ GG+A+P GV + A+ E EV +G +V I Sbjct: 298 FVKAGTVDQVITYLAPKVF-GGAAAPTAIGGDGVIAVADALPFEVQEVIQIGPDVKI 353 >Q8CXN3.1 Q8CXN3_OCEIH Riboflavin specific deaminase (Diaminohydroxyphosphoribosylaminopyrimidine deaminase : 5-amino-6-(5-phosphoribosylamino) uracil reductase) Length = 367 Score = 332 bits (852), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 164/361 (45%), Positives = 240/361 (66%), Gaps = 2/361 (0%) Query: 5 NDEFYMSLALDMAERAQGQTGINPVVGCVVVKDGAMIGLGTHLQRGTGHAEVHALNMAAG 64 +D+ YM LA++ A+ +GQT NP+VG V+V ++G+G+HL+ G HAE+HAL MA Sbjct: 3 DDKHYMKLAIENAKAMKGQTDPNPLVGAVIVNHNRIVGVGSHLKAGEPHAEIHALQMAGE 62 Query: 65 KAQGSTAYVTLEPCSHYGKTPPCSQRLIDEGVARVVVACEDPNPQVAGRGITMLREQGIE 124 A+G+T YVTLEPCSH G+T PC+ L++ GV RVV+A DPNP VAG G+ +L + GIE Sbjct: 63 NAKGATIYVTLEPCSHQGRTGPCALALVEAGVRRVVIATLDPNPLVAGNGVKILEDAGIE 122 Query: 125 VEVGLLRSRALRLNEKFIKYILTKQPFVTLKSASTLDGKLATRTGDSKWISNGQAREIVH 184 VEVG+L ++ +NE F K+I KQPF+T+K+ASTLDGK+AT+T SKWI++ ARE VH Sbjct: 123 VEVGVLEDESISMNEVFNKFITHKQPFITMKAASTLDGKVATKTNHSKWITSEAAREDVH 182 Query: 185 TLRHRHQGIMVGVNTVIADNPSLTTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQAPTI 244 LR+ H I+VG+ T+I D+P+LTTR+ G NP+RIV+DS+LRIPLD+ +++D +APT+ Sbjct: 183 HLRNEHAAILVGIQTIIHDDPALTTRIP-NGRNPIRIVLDSSLRIPLDAQIIQDKRAPTM 241 Query: 245 VVTTESADPVKRAALLDAGVQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGAM 304 + T E D K+ L GV+I+ + + D++ + + + Sbjct: 242 IFTKEEVDQKKKDKLESVGVEIINTSGKEQTDIQDVVNHLGNQQISSLLLEGGGNIHASF 301 Query: 305 LENGLVDRVVLFFAPKIVGGGSASPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCISGTP 364 L GLVD+VVL+ APK+ GG +A P F+ G+E M ++ L L+++ +G + G P Sbjct: 302 LAAGLVDKVVLYMAPKLSGGKNA-PTFFEGAGIEQMDDSIDLLNLKIDQIGPDFRFIGYP 360 Query: 365 I 365 I Sbjct: 361 I 361 >Q65HW4.1 Q65HW4_BACLD Riboflavin-specific deaminase Length = 362 Score = 330 bits (847), Expect = 9e-89, Method: Compositional matrix adjust. Identities = 165/363 (45%), Positives = 233/363 (64%), Gaps = 2/363 (0%) Query: 4 MNDEFYMSLALDMAERAQGQTGINPVVGCVVVKDGAMIGLGTHLQRGTGHAEVHALNMAA 63 M D YM+LAL++A + +GQTG NP+VG VVVKDG ++G+G HL+ G HAEVHA+ MA Sbjct: 1 MTDAHYMNLALELARQGEGQTGANPLVGAVVVKDGEVVGMGAHLKYGEAHAEVHAIQMAG 60 Query: 64 GKAQGSTAYVTLEPCSHYGKTPPCSQRLIDEGVARVVVACEDPNPQVAGRGITMLREQGI 123 A+G+ YVTLEPCSHYGKTPPC++ +I G+ RVVVA EDPNP V+GRGI MLR GI Sbjct: 61 RHAEGAVIYVTLEPCSHYGKTPPCAELIIRSGLKRVVVAAEDPNPLVSGRGIEMLRSAGI 120 Query: 124 EVEVGLLRSRALRLNEKFIKYILTKQPFVTLKSASTLDGKLATRTGDSKWISNGQAREIV 183 EVE G+L+ +A LNEKF+ ++ T P+VTLK+A++LDGK AT TGDSKWI++ +AR Sbjct: 121 EVETGVLKEQAEELNEKFMHFMRTGLPYVTLKAAASLDGKTATATGDSKWITSEEARMDA 180 Query: 184 HTLRHRHQGIMVGVNTVIADNPSLTTRMEVPGLNPVRIVIDSTLRIPLDSAVVRDGQAPT 243 H R HQ I+VG T+ AD+PSLT R+ PVR+V+D+TL IPL S V++D +APT Sbjct: 181 HQYRKNHQCILVGSGTIKADDPSLTCRLPGAVNQPVRVVLDTTLSIPLQSKVIQDKEAPT 240 Query: 244 IVVTTESADPVKRAALLDAGVQIVVSGAGPRVDLKAAMXXXXXXXXXXXXXXXXXXXNGA 303 + TT ADP K + GVQ+ V A + ++ + + + Sbjct: 241 WIYTTSKADPEKVRNVEKEGVQVTVLDAD-ELQVRDVLLHLADRGIASVFVEGGATVHAS 299 Query: 304 MLENGLVDRVVLFFAPKIVGGGSASPGTFDFPGVELMQSAVTLEGLEVEVLGDNVCISGT 363 ++ GL +++ + APK++ GG SP G + M+ L+ + +G ++ ++ Sbjct: 300 FVKEGLYQQIIFYLAPKLI-GGLQSPSILSGEGFQSMKDVPLLQFTTITKIGQDIKLAAR 358 Query: 364 PIR 366 PI+ Sbjct: 359 PIK 361 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,047,700,225 Number of extensions: 81837689 Number of successful extensions: 204990 Number of sequences better than 1.0e-05: 1841 Number of HSP's gapped: 202254 Number of HSP's successfully gapped: 1841 Length of query: 366 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 229 Effective length of database: 1,770,017,148 Effective search space: 405333926892 Effective search space used: 405333926892 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)