BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0035.p (222 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL56.1 B3KL56_9BACI Riboflavin synthase, alpha subunit 323 8e-87 B1DJA9.1 B1DJA9_9BACL Riboflavin synthase, alpha subunit 263 1e-68 C0EYF3.1 C0EYF3_9FIRM Putative uncharacterized protein 233 8e-60 C0ZKW3.1 C0ZKW3_BREBN Riboflavin synthase alpha subunit EC=2.5.1.9 230 6e-59 C1QFI0.1 C1QFI0_9SPIR Riboflavin synthase, alpha subunit 223 1e-56 >B3KL56.1 B3KL56_9BACI Riboflavin synthase, alpha subunit Length = 222 Score = 323 bits (827), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 154/221 (69%), Positives = 184/221 (83%) Query: 1 MFTGLIEEVGVLRGVTSGGEMMVLNIGASLIMDDLKIGDSVSVNGVCLTATSIGDHSFTV 60 MFTGL+EE+G +R + GE M+L+I A+ IMDDLK+GDSVSVNGVCLTAT+I FTV Sbjct: 1 MFTGLVEEIGEMRRIGRQGEAMLLSISANRIMDDLKLGDSVSVNGVCLTATAIDKGVFTV 60 Query: 61 DVMPQTYRNSTLKELRSGSRMNLERAMAAGGRFGGHIVQGHVDGTGEIRSVKRDQNAVVF 120 DVMP+TYRN+ LKEL+ G ++NLERAMAAGGRFGGHIVQGHVDG G IRSVK DQNAVV+ Sbjct: 61 DVMPETYRNTNLKELQPGGKVNLERAMAAGGRFGGHIVQGHVDGIGVIRSVKADQNAVVY 120 Query: 121 EIAPERTSLFKFIIPKGSITIDGISLTVVDTSASAFTVSIIPHTLGETVLAHKRTGDRIN 180 EI+P +FK+IIPKGS+T+DGISLTVV T+ S+FTVSIIPHTL ETVLA+K+ GD IN Sbjct: 121 EISPANGDVFKYIIPKGSVTLDGISLTVVKTTDSSFTVSIIPHTLAETVLAYKKAGDTIN 180 Query: 181 LECDVLGKYVDHLLNYGSRAGNAEEANSSRISRDFLAANGF 221 +ECD+LGKYVDHLL+YGS + SRISRD+LA +GF Sbjct: 181 IECDILGKYVDHLLHYGSEGAGSRSGTKSRISRDYLAEHGF 221 >B1DJA9.1 B1DJA9_9BACL Riboflavin synthase, alpha subunit Length = 223 Score = 263 bits (671), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 130/223 (58%), Positives = 161/223 (72%), Gaps = 1/223 (0%) Query: 1 MFTGLIEEVGVLRGVTSGGEMMVLNIGASLIMDDLKIGDSVSVNGVCLTATSIGDHSFTV 60 MFTGL+EEVG LR V GE MVL + AS + + + +GDS+SVNGVCLT S SF Sbjct: 1 MFTGLVEEVGTLRRVKKQGEAMVLTVEASKVTEGVALGDSISVNGVCLTVISFDRTSFDA 60 Query: 61 DVMPQTYRNSTLKELRSGSRMNLERAMAAGGRFGGHIVQGHVDGTGEIRSVKRDQNAVVF 120 DVMP+TYR S L +LR G +NLERAM AGGRFGGHIVQGHVDG G I + D NAVVF Sbjct: 61 DVMPETYRKSNLHQLRPGQDVNLERAMLAGGRFGGHIVQGHVDGVGMITGRETDANAVVF 120 Query: 121 EIAPERTSLFKFIIPKGSITIDGISLTVVDTSASAFTVSIIPHTLGETVLAHKRTGDRIN 180 I + +++IPKGSITIDGISLTVV T+A+ F+VS+IPHTLGET L +K+ GD +N Sbjct: 121 AIRLSDDEMMRYVIPKGSITIDGISLTVVSTNATGFSVSVIPHTLGETALMNKKAGDSVN 180 Query: 181 LECDVLGKYVDHLLNYGSRAGNAEEANS-SRISRDFLAANGFV 222 +ECDV+GKYVDHLL+Y + + S++S FL+ NGF+ Sbjct: 181 IECDVIGKYVDHLLHYKPAGSSGSSGRTESKLSAAFLSENGFL 223 >C0EYF3.1 C0EYF3_9FIRM Putative uncharacterized protein Length = 235 Score = 233 bits (594), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 120/223 (53%), Positives = 155/223 (69%), Gaps = 3/223 (1%) Query: 1 MFTGLIEEVGVLRGVTSGGEMMVLNIGASLIMDDLKIGDSVSVNGVCLTATSIGDHSFTV 60 MFTG+IEEVG ++ + G V+ I A+ ++ DL +GDSV++NGVCLTATSIG ++++V Sbjct: 14 MFTGIIEEVGEIQSIKKGANSAVITIKANTVLGDLHLGDSVALNGVCLTATSIGKNNYSV 73 Query: 61 DVMPQTYRNSTLKELRSGSRMNLERAMAAGGRFGGHIVQGHVDGTGEIRSVKRDQNAVVF 120 DVM +T R + L EL+SGS++NLERAMAA GRFGGHIV GHVDGTG I S+K+D NAV Sbjct: 74 DVMHETLRRTNLGELKSGSKVNLERAMAANGRFGGHIVAGHVDGTGTISSMKKDDNAVWI 133 Query: 121 EIAPERTSLFKFIIPKGSITIDGISLTVVDTSASAFTVSIIPHTLGETVLAHKRTGDRIN 180 I ++ K+II KGSITIDG+SLTV +F VS+IPHT T L K+ GD +N Sbjct: 134 TI-DTSAAILKYIISKGSITIDGVSLTVAKVDNKSFAVSVIPHTGQNTTLLDKKPGDTVN 192 Query: 181 LECDVLGKYVDHLLNYGS--RAGNAEEANSSRISRDFLAANGF 221 LE D++GKYV+ LL YGS + I+ DFL NGF Sbjct: 193 LENDMVGKYVEKLLQYGSIPAEEEEGTTEKTGITMDFLIQNGF 235 >C0ZKW3.1 C0ZKW3_BREBN Riboflavin synthase alpha subunit EC=2.5.1.9 Length = 218 Score = 230 bits (587), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 121/221 (54%), Positives = 156/221 (70%), Gaps = 4/221 (1%) Query: 1 MFTGLIEEVGVLRGVTSGGEMMVLNIGASLIMDDLKIGDSVSVNGVCLTATSIGDHSFTV 60 MFTGL+EEVGVL + + L I A +++ +K+GDS+SVNG+CLT TS + F+V Sbjct: 1 MFTGLVEEVGVLEAIQGNEQARRLVIRAQQVLEGVKLGDSISVNGICLTVTSYTNRQFSV 60 Query: 61 DVMPQTYRNSTLKELRSGSRMNLERAMAAGGRFGGHIVQGHVDGTGEIRSVKRDQNAVVF 120 DVMP+T ++L++LRSG R+NLERAM G RFGGHIV GHVDGTG + S + + NAVVF Sbjct: 61 DVMPETMNKTSLRQLRSGQRVNLERAMRLGDRFGGHIVSGHVDGTGMVASREVNANAVVF 120 Query: 121 EIAPERTSLFKFIIPKGSITIDGISLTVVDTSASAFTVSIIPHTLGETVLAHKRTGDRIN 180 I + L K++I +GSI IDGISLTVV+ + S FTVSIIPHTL T LA +R GD +N Sbjct: 121 RIEA-KPQLLKYVIARGSICIDGISLTVVEVNDSGFTVSIIPHTLANTSLADRRPGDLVN 179 Query: 181 LECDVLGKYVDHLLNYGSRAGNAEEANSSRISRDFLAANGF 221 LE DV+GKYV+ LL Y + G+ E+ S +S FL NGF Sbjct: 180 LETDVIGKYVERLLGYQNH-GSTEK--SGGLSLAFLQENGF 217 >C1QFI0.1 C1QFI0_9SPIR Riboflavin synthase, alpha subunit Length = 217 Score = 223 bits (567), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 108/222 (48%), Positives = 153/222 (68%), Gaps = 6/222 (2%) Query: 1 MFTGLIEEVGVLRGVTSGGEMMVLNIGASLIMDDLKIGDSVSVNGVCLTATSIGDHSFTV 60 MFTG++EE+G+++ V S V+ I AS I DDL +GDSV+VNG CLT +S + F Sbjct: 1 MFTGIVEEIGIVKSVQSK----VITIEASKIFDDLHLGDSVAVNGTCLTVSSFDNKIFNA 56 Query: 61 DVMPQTYRNSTLKELRSGSRMNLERAMAAGGRFGGHIVQGHVDGTGEIRSVKRDQNAVVF 120 DV +T + L L++GSR+NLERAM GRFGGHIV GH+DG G I+S+K+D NA++ Sbjct: 57 DVTQETLNRTNLGSLKNGSRVNLERAMTLSGRFGGHIVSGHIDGVGSIKSMKKDDNAIIL 116 Query: 121 EIAPERTSLFKFIIPKGSITIDGISLTVVDTSASAFTVSIIPHTLGETVLAHKRTGDRIN 180 I R L K+I+ KGS+ +DGISLTV + + F++++IPHTL ETVL +K+ GD++N Sbjct: 117 TIEVPR-QLMKYIVEKGSVAVDGISLTVASLTDNTFSIAVIPHTLKETVLYYKKEGDKVN 175 Query: 181 LECDVLGKYVDHLLNYGS-RAGNAEEANSSRISRDFLAANGF 221 +E DV+GKYV+ LL + N+ ++ S I+ +FL NGF Sbjct: 176 IENDVIGKYVERLLTFKEDDKENSNDSKKSNITMEFLLKNGF 217 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,243,607,060 Number of extensions: 49128963 Number of successful extensions: 130500 Number of sequences better than 1.0e-05: 1538 Number of HSP's gapped: 124941 Number of HSP's successfully gapped: 1538 Length of query: 222 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 91 Effective length of database: 1,826,543,238 Effective search space: 166215434658 Effective search space used: 166215434658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)