BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0027.p (172 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL62.1 B3KL62_9BACI Sporulation protein YtfJ 200 5e-50 C0ZH16.1 C0ZH16_BREBN Putative uncharacterized protein ytfJ 158 2e-37 Q2B3D5.1 Q2B3D5_9BACI Putative uncharacterized protein 155 1e-36 Q65G62.1 Q65G62_BACLD YtfJ 154 2e-36 B1DJA0.1 B1DJA0_9BACL Sporulation protein YtfJ 153 5e-36 >B3KL62.1 B3KL62_9BACI Sporulation protein YtfJ Length = 149 Score = 200 bits (508), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 103/145 (71%), Positives = 113/145 (77%), Gaps = 3/145 (2%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVE- 59 M+EHPIQGLMQTAMENIKGMVDVNTIVGDPV TPDGSVILPISKVAFGFAAGGSDY VE Sbjct: 1 MAEHPIQGLMQTAMENIKGMVDVNTIVGDPVNTPDGSVILPISKVAFGFAAGGSDYNVEA 60 Query: 60 --DDAPGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIID 117 NG + LPF VSIRPIAFLVVG+EGVHIVPLDNQTHLFE+IID Sbjct: 61 GGTHHNTANGTHTDHNALPFGGGSGGGVSIRPIAFLVVGKEGVHIVPLDNQTHLFERIID 120 Query: 118 ATPGLIDKIQTMFPSSGSSQNDASP 142 ATP L+D+IQ+MF S ++ +P Sbjct: 121 ATPYLLDQIQSMFQSGSNAAGSTTP 145 >C0ZH16.1 C0ZH16_BREBN Putative uncharacterized protein ytfJ Length = 161 Score = 158 bits (399), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 76/134 (56%), Positives = 99/134 (73%), Gaps = 1/134 (0%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVED 60 M++HPIQGLM+TAMEN+K MVDVNTI+GDPVETPDGSVILPISKV FGFAAGGS+++ + Sbjct: 1 MADHPIQGLMRTAMENLKQMVDVNTIIGDPVETPDGSVILPISKVGFGFAAGGSEFQYDH 60 Query: 61 DAPGINGAGSGVKML-PFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIIDAT 119 G PF VSI P+AFLVVG++G+ +PL+N THL+++I+D+ Sbjct: 61 HHNGHQHTQQHEHTANPFGGGSGGGVSITPVAFLVVGKQGIRSIPLENTTHLYDRILDSV 120 Query: 120 PGLIDKIQTMFPSS 133 P ++DK+Q MF S Sbjct: 121 PQIVDKVQGMFTKS 134 >Q2B3D5.1 Q2B3D5_9BACI Putative uncharacterized protein Length = 168 Score = 155 bits (392), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 84/149 (56%), Positives = 99/149 (66%), Gaps = 6/149 (4%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVED 60 MS+HPIQGLM TAMEN+K M+DVNTI+GDPVETPDGSVIL +SKV FGFAAGGS++ +E Sbjct: 7 MSDHPIQGLMTTAMENLKEMIDVNTIIGDPVETPDGSVILTVSKVGFGFAAGGSEFMLE- 65 Query: 61 DAPGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIIDATP 120 G G G PF VSI PIAFL+V GV +V LD THL+EKI+D P Sbjct: 66 ---GSGREGEGKPKHPFGGGSGGGVSITPIAFLIVSSHGVKMVHLDESTHLYEKILDLAP 122 Query: 121 GLIDKIQTMFPSSGSSQNDASPQNGTPVG 149 +DKIQ M + S N S Q+G G Sbjct: 123 QAVDKIQQMMSKNNGSGN--SSQDGKSEG 149 >Q65G62.1 Q65G62_BACLD YtfJ Length = 152 Score = 154 bits (390), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 79/152 (51%), Positives = 104/152 (68%), Gaps = 7/152 (4%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVED 60 M+EHPIQGLM+TAMEN+K M+DVNTI+GDPVETPDGSVIL +S+V FGFAAGGS++ Sbjct: 1 MAEHPIQGLMKTAMENLKEMIDVNTIIGDPVETPDGSVILTVSRVGFGFAAGGSEFG--G 58 Query: 61 DAPGINGAGSGVK-----MLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKI 115 + P + A G + LPF VSI PIAFL+VG GV ++ LD TH+ +KI Sbjct: 59 NKPAESKASDGDRERKESKLPFGGGSGGGVSITPIAFLIVGSSGVKVLHLDENTHVIDKI 118 Query: 116 IDATPGLIDKIQTMFPSSGSSQNDASPQNGTP 147 +D+ P I++IQ MF + + + + QN TP Sbjct: 119 LDSAPQTIERIQHMFKKNDKQKQNMNRQNQTP 150 >B1DJA0.1 B1DJA0_9BACL Sporulation protein YtfJ Length = 157 Score = 153 bits (387), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 87/147 (59%), Positives = 101/147 (68%), Gaps = 8/147 (5%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVE- 59 M EHPIQGLMQTAMENIK MVDVNTIVGDPV+TPDGS+I+PISKV FGF AGGSD R + Sbjct: 1 MPEHPIQGLMQTAMENIKEMVDVNTIVGDPVQTPDGSIIMPISKVGFGFVAGGSDIRFDG 60 Query: 60 ------DDAPGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFE 113 + + + PF VSI PIAFLVVG GV IVPLDNQTHL E Sbjct: 61 SASHDHSSSSDGHNQHNAGAAGPFGGGSGGGVSITPIAFLVVGTHGVKIVPLDNQTHLME 120 Query: 114 KIIDATPGLIDKIQTMF-PSSGSSQND 139 +IID+ P + DK+Q MF S+G ++D Sbjct: 121 RIIDSAPVVFDKVQHMFRTSAGKHEHD 147 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,055,182,568 Number of extensions: 44528834 Number of successful extensions: 78585 Number of sequences better than 1.0e-05: 318 Number of HSP's gapped: 78246 Number of HSP's successfully gapped: 318 Length of query: 172 Length of database: 3,060,696,203 Length adjustment: 126 Effective length of query: 46 Effective length of database: 1,873,648,313 Effective search space: 86187822398 Effective search space used: 86187822398 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 126 (53.1 bits)