BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0026.p (400 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL63.1 B3KL63_9BACI Serine-type D-Ala-D-Ala carboxypeptidase E... 441 e-122 B1DJ99.1 B1DJ99_9BACL Serine-type D-Ala-D-Ala carboxypeptidase E... 404 e-111 C0ZC78.1 C0ZC78_BREBN Peptidase S11 family protein EC=3.4.16.4 330 2e-88 A6CMY5.1 A6CMY5_9BACI D-alanyl-D-alanine carboxypeptidase (Penic... 283 2e-74 C5D3M0.1 C5D3M0_9BACI Serine-type D-Ala-D-Ala carboxypeptidase E... 278 7e-73 >B3KL63.1 B3KL63_9BACI Serine-type D-Ala-D-Ala carboxypeptidase EC=3.4.16.4 Length = 394 Score = 441 bits (1133), Expect = e-122, Method: Compositional matrix adjust. Identities = 223/356 (62%), Positives = 261/356 (73%), Gaps = 3/356 (0%) Query: 37 TTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAK 96 + HA+AAALIDV SGR+LYS GDE + IASLTKIMTA+VAIE+G L +VKV KNAFAK Sbjct: 38 SNHAQAAALIDVTSGRMLYSKNGDERLRIASLTKIMTAIVAIEHGKLDDRVKVSKNAFAK 97 Query: 97 EGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQH 156 EGSS+FLK GEEM LE++LYGLMLRSGNDAATAIAEHVGG+EEGFV LMN KA LG+ + Sbjct: 98 EGSSIFLKLGEEMTLENLLYGLMLRSGNDAATAIAEHVGGTEEGFVLLMNEKAASLGMTN 157 Query: 157 THFTNPHGLDAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNKN 216 +HF NPHGLD + HYSSA+D+A LTAYA+ NPVF +IVKT K A NP E WDYKW NKN Sbjct: 158 SHFMNPHGLDHDEHYSSAHDVAKLTAYALKNPVFSEIVKTPTKKAPNPNEAWDYKWDNKN 217 Query: 217 KMLRLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFNHY 276 KMLR YEGADGVKTGYTKKA RCLVSSATR GQQ+ AVTLNDGNDWNDH+ +LDYGF H+ Sbjct: 218 KMLRFYEGADGVKTGYTKKAFRCLVSSATRNGQQIAAVTLNDGNDWNDHSNMLDYGFAHF 277 Query: 277 PLKTLVERGEGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNTRRSSFGIKGALV 336 PLK + E+G+ I GY V +F Y EEARLV +L L +T SFG++G + Sbjct: 278 PLKKVTEKGQKIQGYPLVTGSSFTYAFAHDEEARLVKRLVL--HKASTPDLSFGLRGHIE 335 Query: 337 LQLGGKEIGRVPVYMPDRLPPEQSPYEKRFQHAGAAAYPADNWLQALGSALRALFQ 392 L L IG+VPVY PE ++ + A W Q+ G LR LF+ Sbjct: 336 LLLDNVSIGKVPVYAEGSKLPEAPEALQKSRSALGPVGSLSLW-QSAGEVLRHLFR 390 >B1DJ99.1 B1DJ99_9BACL Serine-type D-Ala-D-Ala carboxypeptidase EC=3.4.16.4 Length = 393 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 205/362 (56%), Positives = 255/362 (70%), Gaps = 13/362 (3%) Query: 38 THARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAKE 97 T A +AL+DV SGR+LYS GD+ M IASLTKIMTA+VAIE+G L +VK A KE Sbjct: 37 TSANGSALVDVKSGRILYSENGDQSMRIASLTKIMTAIVAIEHGKLSERVKTSSRAVGKE 96 Query: 98 GSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQHT 157 GSS++L+ GEEM L +MLYGLMLRSGNDAATAIAEHVGGSEEGFV+LMN KAE LGL HT Sbjct: 97 GSSIYLQLGEEMTLLNMLYGLMLRSGNDAATAIAEHVGGSEEGFVHLMNEKAEMLGLTHT 156 Query: 158 HFTNPHGLDAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNKNK 217 F NPHGLD EGHYSSANDLA LTAYA+ NPVF +IV T+VK+ NP+E W Y W NKNK Sbjct: 157 QFKNPHGLDVEGHYSSANDLAKLTAYALRNPVFAEIVSTKVKSVPNPHEKWQYSWTNKNK 216 Query: 218 MLRLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFNHYP 277 ML +YEGADGVKTGYTKKALRCLVSSATR GQQL AVTLND +DW DH +LD+GF HYP Sbjct: 217 MLAMYEGADGVKTGYTKKALRCLVSSATRDGQQLAAVTLNDRDDWVDHRQMLDWGFKHYP 276 Query: 278 LKTLVERGEGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNTRRSSFGIKGALVL 337 L + +G+ I+GY +F YPL + E L ++L ++ + T G +GAL Sbjct: 277 LMEVASKGQTIAGYPLAVGNSFYYPLAEEERGSLQSRLIVL--DMATMNYKLGERGALEW 334 Query: 338 QLGGKEIGRVPVY--------MPDRLPPEQSPYEKRFQHAGAAAYPADNWLQALGSALRA 389 L ++IG +PVY +P+R P + ++ A+ ++++ ++ + + A Sbjct: 335 YLNDQKIGSIPVYDIGSARMSLPERKPMSDAALLLDYKK---ASREHESFIHSITAVIGA 391 Query: 390 LF 391 LF Sbjct: 392 LF 393 >C0ZC78.1 C0ZC78_BREBN Peptidase S11 family protein EC=3.4.16.4 Length = 389 Score = 330 bits (845), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 176/336 (52%), Positives = 223/336 (66%), Gaps = 11/336 (3%) Query: 40 ARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAKEGS 99 A AALIDV SGR+LYS G + M IASLTK MTA+VAIE G L ++V V A EGS Sbjct: 38 AEGAALIDVESGRILYSKNGTKKMRIASLTKTMTAIVAIEMGKLDAQVTVPPQAVGVEGS 97 Query: 100 SLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQHTHF 159 S++LK GE++ LE++LYGLMLRSGNDAA IA H+GGS GF YLMN KA +G+++T+F Sbjct: 98 SIYLKNGEKLTLEELLYGLMLRSGNDAAVTIAMHIGGSVPGFTYLMNEKAAMIGMEYTNF 157 Query: 160 TNPHGL-DAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNKNKM 218 TNPHGL D+ HYS+ D+A L+AYA+HNPVF+QIV T+VK E WD + LNKNKM Sbjct: 158 TNPHGLDDSNQHYSTPEDMAKLSAYALHNPVFRQIVSTKVKDISWEGEQWDRRLLNKNKM 217 Query: 219 LRLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFNHYPL 278 L LY GADGVKTGYTK A RCL SSATR G+QL +TLN +DWND A L+D+GF +YPL Sbjct: 218 LHLYNGADGVKTGYTKLAKRCLASSATRNGRQLATITLNASDDWNDSAKLMDWGFANYPL 277 Query: 279 KTLVERGEGI---------SGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNTRRSSF 329 + LV+ G+ + +G V F YPL Q E + K ++ E+ T + + Sbjct: 278 QELVKNGQEVKPDTPVTLEAGTHLVTTNAFQYPL-QTAEIEAIHKRVVMGESTVTSKMNG 336 Query: 330 GIKGALVLQLGGKEIGRVPVYMPDRLPPEQSPYEKR 365 + G L L L K IG+VP+ + P +P R Sbjct: 337 QLAGFLQLYLKEKRIGQVPLLVRVDTPTLAAPQASR 372 >A6CMY5.1 A6CMY5_9BACI D-alanyl-D-alanine carboxypeptidase (Penicillin-binding protein) Length = 384 Score = 283 bits (724), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 149/324 (45%), Positives = 211/324 (65%), Gaps = 11/324 (3%) Query: 37 TTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAK 96 + A+ A +ID +GR+LY P IAS+TKIMTA++AIE+G L VKV NA Sbjct: 32 SVSAQRAIVIDQDTGRVLYEKDAHTPSRIASITKIMTAILAIESGKLEETVKVSNNAVKA 91 Query: 97 EGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQH 156 EGSS++LK GE+++LED++YGLMLRSGND+A AIAEHVGGS +GF++LMN KAEE+G+++ Sbjct: 92 EGSSIYLKPGEKIKLEDLVYGLMLRSGNDSAVAIAEHVGGSLDGFIFLMNKKAEEIGMRN 151 Query: 157 THFTNPHGLDA-EGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNK 215 THF NPHGLD E HYS+A D+A+LT YAM N +K++ T+V T+ +P WD KW NK Sbjct: 152 THFANPHGLDDHEDHYSTAFDMALLTKYAMQNEKYKEVAGTKVHTSPDPEHDWDRKWKNK 211 Query: 216 NKML-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFN 274 N++L LY+ G KTGYTK+A R LV++AT+G Q L+AVTLN +DWNDH + ++GF Sbjct: 212 NRLLTELYKYCTGGKTGYTKRAKRTLVTTATKGEQNLIAVTLNGPDDWNDHIQMFEFGFK 271 Query: 275 HYPLKTLVERG-------EGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNTRRS 327 ++ V G E ++ R L + EE + + +L++ + R Sbjct: 272 NFDTVIAVPEGNVKKIEDEFYKNKVYI-KRDVLLTLTKEEEESVRVEYKLMKPEDSWRDG 330 Query: 328 SF-GIKGALVLQLGGKEIGRVPVY 350 + + G V+ + KE VP++ Sbjct: 331 AVPDVVGKAVIYMNEKEADAVPLF 354 >C5D3M0.1 C5D3M0_9BACI Serine-type D-Ala-D-Ala carboxypeptidase EC=3.4.16.4 Length = 381 Score = 278 bits (711), Expect = 7e-73, Method: Compositional matrix adjust. Identities = 151/331 (45%), Positives = 213/331 (64%), Gaps = 12/331 (3%) Query: 40 ARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAKEGS 99 A++A L++ SGR+L+ IAS+TKIMT ++AIE+G + V V A EGS Sbjct: 33 AQSAILMEQNSGRILFEKDAHTKRRIASITKIMTVILAIESGKMDDTVTVSARAVHTEGS 92 Query: 100 SLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQHTHF 159 S++LK+GE+++L D++YG MLRSGNDAA AIAEHVGGS EGFV+LMN KA E+G+++THF Sbjct: 93 SIYLKEGEKIKLRDLVYGTMLRSGNDAAVAIAEHVGGSVEGFVFLMNQKAAEIGMRNTHF 152 Query: 160 TNPHGL-DAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTAD-NPYEPWDYKWLNKNK 217 NPHGL D+E HYS+A D+A+L YAM N F++I T+V A +P E WD W NKNK Sbjct: 153 ANPHGLDDSENHYSTAYDMALLMQYAMKNKEFRKISGTEVYRAPASPGEDWDRVWRNKNK 212 Query: 218 ML-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFNHY 276 +L LYE G KTGYTK+A R LV++A++GG L++VTL+ +DWNDH + +YGF HY Sbjct: 213 LLTSLYEYCTGGKTGYTKRAKRTLVTTASKGGMDLISVTLDAPDDWNDHIAMYEYGFGHY 272 Query: 277 PL-----KTLVER-GEGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNTRRSSFG 330 + K +V++ + + +R YP+ + E L K+ L+R K+ ++ G Sbjct: 273 MIAKVNRKRMVKKIRDSFYRKNIQASRDLRYPVTEEEIKELHVKIYLLRPKKDWKKDERG 332 Query: 331 IK---GALVLQLGGKEIGRVPVYMPDRLPPE 358 I G VL LG + + VP+ + E Sbjct: 333 IPTVVGKAVLYLGDQAVDEVPLLYKKSVSKE 363 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,222,105,458 Number of extensions: 92803730 Number of successful extensions: 228693 Number of sequences better than 1.0e-05: 3172 Number of HSP's gapped: 222576 Number of HSP's successfully gapped: 3175 Length of query: 400 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 262 Effective length of database: 1,760,596,133 Effective search space: 461276186846 Effective search space used: 461276186846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)