BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0019.p (517 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL69.1 B3KL69_9BACI ResB family protein 740 0.0 B1DJ93.1 B1DJ93_9BACL ResB family protein 612 e-173 P35161.1 RESB_BACSU Cytochrome c biogenesis protein resB 389 e-106 Q9KCJ3.1 Q9KCJ3_BACHD Cytochrome c biogenesis 387 e-105 A7Z663.1 A7Z663_BACA2 ResB 384 e-105 >B3KL69.1 B3KL69_9BACI ResB family protein Length = 567 Score = 740 bits (1910), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/516 (67%), Positives = 419/516 (81%), Gaps = 1/516 (0%) Query: 1 MRYDGVARRSQRVNPGVIDLVWNFFSSVKIAIYLIVLTLLGSMLGTIFPQESTFLNIDAS 60 MRYDGVARRSQ+ NPG+ID VWNFFSSVK+AIYLI+LTL+GS LGTIFPQESTFLNID S Sbjct: 46 MRYDGVARRSQKENPGIIDRVWNFFSSVKVAIYLILLTLIGSSLGTIFPQESTFLNIDPS 105 Query: 61 TYYPEKYGTAGEIYYKLGLSHTYESWWFVLLLVLIGASLVICSLDRVLPLYKALTRQKIR 120 YY E+YG G IYY LGLSHTY SWWFVLLLV+IGASLVICSLDRVLPLYKAL+RQ+I Sbjct: 106 VYYKEEYGQLGHIYYLLGLSHTYTSWWFVLLLVMIGASLVICSLDRVLPLYKALSRQRIP 165 Query: 121 KHRQFLTRQKVALVAELKEEPEAWVARMVEPLQKKGYRVRTDGGALLAEKHRFSRWGPYV 180 KH FLTRQKV +++E+ WV R+ ++KKGYRVRTDGG LLAEK RFSRWGPYV Sbjct: 166 KHMSFLTRQKVVYQGQVQEDGADWVERIKPIIKKKGYRVRTDGGTLLAEKQRFSRWGPYV 225 Query: 181 IHIGLIIFLLAVLARGLPGLNMDQHLAFPQGDTVRIPDTTYYLKNEKFTVEFYTEEEMPK 240 +HIGLIIFLLAVL R LPGL+MD+H+AFPQ + V I +T+YYL N+ F VEFYTE++MP+ Sbjct: 226 LHIGLIIFLLAVLGRNLPGLHMDEHMAFPQDEPVHIKNTSYYLLNDGFKVEFYTEDDMPE 285 Query: 241 EFRG-TKILPKLYETKAVLYECTADCTDPSKEPQLAEVARHNIQVNSPLSYNGLKAYQFD 299 EF+G K+LPK ++T+A+LY C A+C D KEPQL E+ RHNI+VN PL+Y GLKAYQFD Sbjct: 286 EFKGKNKVLPKEFQTQAILYMCEANCDDLDKEPQLVELTRHNIEVNHPLNYKGLKAYQFD 345 Query: 300 YDLTPVLRSVQPELINAKSGEVYGKFKLDMNNPKRTFQAGPYTLSLKEKYMDFGLNEEGQ 359 YDLTP+LR+V P LI++KSG+ YGK L +N+P+R +QAG YTL+L + + DFGL + G+ Sbjct: 346 YDLTPILRTVSPVLIDSKSGKEYGKITLSINDPQREYQAGEYTLTLTQSFTDFGLGDNGK 405 Query: 360 PVSKSPYPNAPAFLFLITGPGLPAGGQQYFYFPKQVDKEKFQMTAINDKLGGSGRFLELE 419 PV+ SP PNAPAFLFLI GP LP G QYFYFPKQ+DKE+F AIN +L G ++L+ Sbjct: 406 PVNLSPNPNAPAFLFLIKGPDLPKEGVQYFYFPKQIDKERFNQEAINAQLAGGEALVKLD 465 Query: 420 VGDMSDVDFSESTTYLNIRVDRAMPFVWIGAGIVMLGLILGFYWQHRRIWLAANHGELVL 479 V M DVDFSESTTYLNIRVD+AMPF+W+G I M+GL++GFY+ HRRIWL ++G L L Sbjct: 466 VLSMDDVDFSESTTYLNIRVDKAMPFIWVGCFIGMIGLVMGFYFTHRRIWLRIDNGTLTL 525 Query: 480 GGHTNKNWFGFRREIVSILAKTDLTVDEKSLDNGGG 515 G HTNKNWFG R+EI IL + ++ VDEKSL+N G Sbjct: 526 GAHTNKNWFGLRKEISGILKQMNIEVDEKSLENNRG 561 >B1DJ93.1 B1DJ93_9BACL ResB family protein Length = 520 Score = 612 bits (1577), Expect = e-173, Method: Compositional matrix adjust. Identities = 299/515 (58%), Positives = 386/515 (74%), Gaps = 6/515 (1%) Query: 5 GVARRSQRVNPGVIDLVWNFFSSVKIAIYLIVLTLLGSMLGTIFPQESTFLN-IDASTYY 63 GVARRSQ+ NP ID +WNFFSSVKIA+YLIV+TL+ +MLGTI+PQE +FLN S YY Sbjct: 10 GVARRSQKSNPNPIDKIWNFFSSVKIAVYLIVITLVTAMLGTIYPQEGSFLNNFKPSVYY 69 Query: 64 PEKYGTAGEIYYKLGLSHTYESWWFVLLLVLIGASLVICSLDRVLPLYKALTRQKIRKHR 123 E YG G+IYYKLGLSHTY+SWWF+ LLV+IG SLVICSLDRVLPLY+AL++Q+IRKH Sbjct: 70 EETYGLPGKIYYKLGLSHTYDSWWFITLLVMIGTSLVICSLDRVLPLYRALSKQQIRKHL 129 Query: 124 QFLTRQKVALVAELKEEPEAWVARMVEPLQKKGYRVRTDGGALLAEKHRFSRWGPYVIHI 183 +F+TRQK ++++ + E WV + L+KK Y++ TDG A+LAEK+RFSRWGPY+ HI Sbjct: 130 RFITRQKAVYNSQVEGDAEEWVKQFSAHLKKKRYKIHTDGTAMLAEKNRFSRWGPYINHI 189 Query: 184 GLIIFLLAVLARGLPGLNMDQHLAFPQGDTVRIPDTTYYLKNEKFTVEFYTEEEMPKEFR 243 GLI+FLLA+LAR LPG MD +++ P GDTV+I T Y++KNEKFTVE+YT++E+P E + Sbjct: 190 GLILFLLAILARSLPGWQMDSYVSIPDGDTVQIEGTNYFVKNEKFTVEYYTDDELPPELK 249 Query: 244 GTKILPKLYETKAVLYECTADCTD-PSKEPQLAEVARHNIQVNSPLSYNGLKAYQFDYDL 302 GT + K YETKAVLY+CT +C D + EP+L +V I+VN PL Y+G+K YQFDYD+ Sbjct: 250 GT-VRAKRYETKAVLYKCTDNCGDNATGEPELKQVKEQEIRVNHPLKYDGIKLYQFDYDV 308 Query: 303 TPVLRSVQPELINAKSGEVYGKFKLDMNNPKRTFQAGPYTLSLKEKYMDFGLNEEGQPVS 362 TP L+SV P L+N ++GE YG F+L M + + GPY + L YM+FG+ E+G+PV+ Sbjct: 309 TPKLKSVNPVLMNKETGESYGPFELPMKDAPLDYTLGPYKMKLAANYMEFGIGEDGEPVT 368 Query: 363 KSPYPNAPAFLFLITGPGLPAGGQQYFYFPKQVDKEKFQMTAINDKLGGSGRFLELEVGD 422 S PNAPAF+FLITGPGLP GQ Y YFP Q DKEKF +N + LE++V Sbjct: 369 LSRDPNAPAFIFLITGPGLPEKGQPYIYFPMQKDKEKFDQDKLNASIADK---LEIKVNG 425 Query: 423 MSDVDFSESTTYLNIRVDRAMPFVWIGAGIVMLGLILGFYWQHRRIWLAANHGELVLGGH 482 M +V F+E++TYLN+R D+A+ +VW GA I MLGL+LG YWQHRRIWL + G+L LG H Sbjct: 426 MENVHFAEASTYLNVRKDKALTYVWYGAAISMLGLLLGSYWQHRRIWLRIDDGKLSLGAH 485 Query: 483 TNKNWFGFRREIVSILAKTDLTVDEKSLDNGGGLA 517 TNKNW+G R ++ L K+ + VD KSLDNGG A Sbjct: 486 TNKNWYGMRADVAYALRKSGIDVDPKSLDNGGNNA 520 >P35161.1 RESB_BACSU Cytochrome c biogenesis protein resB Length = 542 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 221/530 (41%), Positives = 312/530 (58%), Gaps = 48/530 (9%) Query: 1 MRYDGVARRSQRVNPGVIDLVWNFFSSVKIAIYLIVLTLLGSMLGTIFPQESTFL-NIDA 59 MRYDG ARRSQ N +ID +WNFFSSVK+ I+LIV+TL S GTIFPQE+ A Sbjct: 36 MRYDGSARRSQTYNKTIIDKIWNFFSSVKVGIWLIVITLAASAFGTIFPQEAYLPPGAQA 95 Query: 60 STYYPEKYGTAGEIYYKLGLSHTYESWWFVLLLVLIGASLVICSLDRVLPLYKALTRQKI 119 TYY E+YGT G++YY LG H Y SWW++LL+ IG SLVICSLDRV+PLY+AL Q + Sbjct: 96 DTYYKEQYGTFGQLYYLLGFHHLYGSWWYLLLIASIGISLVICSLDRVIPLYRALKNQGV 155 Query: 120 RKHRQFLTRQKV--ALVAELKEEPEAWVARMVEPLQKKGYRVRTDGGALLAEKHRFSRWG 177 R+ FL RQ++ V L E + ++V L+KK YR+R G++LAEK RFSRWG Sbjct: 156 RRSPAFLRRQRLFSETVTVLNGESK---EKIVTLLKKKHYRIREKEGSILAEKGRFSRWG 212 Query: 178 PYVIHIGLIIFLLAVLARGLPGLNMDQHLAFPQGDTVRIP--DTTYYLKNEKFTVEFYTE 235 PYV HIGLIIFL+ + R +PG+ +D+ L +G+T IP D YYLKN +F+VE Y Sbjct: 213 PYVNHIGLIIFLIGAMLRFVPGMYVDETLWVREGETAAIPGTDGKYYLKNNQFSVETYNS 272 Query: 236 EEMPKEFR------GTKILPKLYETKAVLYECTADCTDPSKEPQLAEVARHNIQVNSPLS 289 + K F G + K ++T AVLY+ K P+L +V +I+VN PL Sbjct: 273 KTEKKVFADAIDRVGDGRVAKNFQTDAVLYKREGKIVYGEK-PKLEKVTEEDIRVNQPLR 331 Query: 290 YNGLKAYQFDYDLTPVLRSVQPELINAKSGEVYGKFKLDMNNPKRTFQAGP-YTLSLKEK 348 ++ YQ DY + + V +LI+ K+ + +G K+++ +P + G Y + + Sbjct: 332 FDSFSVYQVDYKENQLDQMV-FQLIDKKTKKSFGSLKINLLDPDSVYDLGNGYKVEIASY 390 Query: 349 YMDFGLNEEGQPVSKSPYPNAPAFLFLITGPGLPAGGQQYFYFPKQVDKEKFQMTAINDK 408 DF N++G+P +K+ PN PAF+F I P P G + + AI + Sbjct: 391 LPDFYFNQDGEPSTKTKIPNNPAFVFNIITPDKPKGEKSF--------------VAIQET 436 Query: 409 LGGSG------RFLELEVGDMSDVDFSESTTYLNIRVDRAMPFVWIGAGIVMLGLILGFY 462 + GSG +F +E +++ T L +R D + + +G I M+G+I G Y Sbjct: 437 IEGSGNNKYKLKFDHVE---------TKNITGLTVRKDLTLWVLAVGGAIFMIGVIQGMY 487 Query: 463 WQHRRIWLAANHGELVLGGHTNKNWFGFRREIVSILAKTDLT--VDEKSL 510 WQHRRIWL + G +++ GHTNKNWFG ++++ ILA + LT VD+K L Sbjct: 488 WQHRRIWLHSQDGAVMVAGHTNKNWFGLKKDLAFILADSGLTEPVDQKEL 537 >Q9KCJ3.1 Q9KCJ3_BACHD Cytochrome c biogenesis Length = 556 Score = 387 bits (994), Expect = e-105, Method: Compositional matrix adjust. Identities = 216/514 (42%), Positives = 308/514 (59%), Gaps = 35/514 (6%) Query: 1 MRYDGVARRSQRVNPGVIDLVWNFFSSVKIAIYLIVLTLLGSMLGTIFPQESTF-LNIDA 59 MRY+GVARRSQ ID +W FFSSVK+ I++IVLTL+ S+ GTIFPQE N+ Sbjct: 38 MRYEGVARRSQTYTTTFIDKIWMFFSSVKVGIWIIVLTLVASIFGTIFPQEMYIPSNVRP 97 Query: 60 STYYPEKYGTAGEIYYKLGLSHTYESWWFVLLLVLIGASLVICSLDRVLPLYKALTRQKI 119 T+Y E+YG G+IYY+LG + Y SWW++ L+ +G SL+ICSLDRV+PLY+AL Q++ Sbjct: 98 ETHYAEEYGVLGQIYYQLGFHNLYGSWWYMALIAALGVSLIICSLDRVVPLYRALKTQRV 157 Query: 120 RKHRQFLTRQKVALVAELKEEPEAWVARMVEPLQKKGYRVRTDGGALLAEKHRFSRWGPY 179 +H FL RQ++ + + + + + L++K Y+VR + G LLAEK RFSRWGPY Sbjct: 158 TRHENFLKRQRLFGTSNVN-NVDGVLETVKARLEEKKYKVREENGNLLAEKGRFSRWGPY 216 Query: 180 VIHIGLIIFLLAVLARGLPGLNMDQHLAFPQGDTVRIPDTT--YYLKNEKFTVEFYTEE- 236 V HIGLIIFL+ + R PG+ +D+++ +G+ + +P T YYL NE FT+E Y EE Sbjct: 217 VNHIGLIIFLMGCMFRFFPGMYVDENMWIREGEVMVVPGTNSKYYLGNESFTLELYDEED 276 Query: 237 EMPKEF--RGTKILPKLYETKAVLYECTADCTDPSKEPQLAEVARHNIQVNSPLSYNGLK 294 E+ E R + K YET+AVLYE T +L EVARHNI+VN PLSY GL Sbjct: 277 ELFAEAIERAGGAVAKNYETEAVLYERDPGAT-VGATSELQEVARHNIRVNDPLSYEGLS 335 Query: 295 AYQFDYDLTPVLRSVQPELINAK-SGEVYGKFKLDMNNPKRTFQAGP-YTLSLKEKYMDF 352 YQ DY L SV +N K +GE G+ +D+NNP+ + G + L+E + D+ Sbjct: 336 IYQIDYKLHEF--SVMSFTLNKKETGESLGQIDVDLNNPEDEYDLGNGAKVILREYFPDY 393 Query: 353 GLNEEGQPVSKSPYPNAPAFLFLITGPGLPAGGQQYFYFPKQVD---KEKFQMTAINDKL 409 LNE+ P ++S P+ P F+F + P P G + + ++ + +++M Sbjct: 394 ELNEQNVPSTRSRVPDNPGFIFEMITPETPEGEISFVAVQQNLEPLGENQYEM------- 446 Query: 410 GGSGRFLELEVGDMSDVDFSESTTYLNIRVDRAMPFVWIGAGIVMLGLILGFYWQHRRIW 469 +F +LE + T L +R D +PF+++G I M+GLI G YW HRR+W Sbjct: 447 ----KFADLE---------TTHVTGLTVRKDYTLPFLFVGGFIFMVGLIQGSYWAHRRVW 493 Query: 470 LAANHGELVLGGHTNKNWFGFRREIVSILAKTDL 503 L +GE+ + GHTNKNW +EI +A+T L Sbjct: 494 LQRVNGEVWIAGHTNKNWSSLHKEIDEAIAETGL 527 >A7Z663.1 A7Z663_BACA2 ResB Length = 542 Score = 384 bits (987), Expect = e-105, Method: Compositional matrix adjust. Identities = 211/522 (40%), Positives = 310/522 (59%), Gaps = 32/522 (6%) Query: 1 MRYDGVARRSQRVNPGVIDLVWNFFSSVKIAIYLIVLTLLGSMLGTIFPQESTFL-NIDA 59 MRYDG ARRSQ N ++D VWNFFSSVK+ I+LIV+TL S GT+FPQE+ Sbjct: 36 MRYDGSARRSQTYNKTIVDKVWNFFSSVKVGIWLIVITLAASAFGTVFPQEAYLPPGAQP 95 Query: 60 STYYPEKYGTAGEIYYKLGLSHTYESWWFVLLLVLIGASLVICSLDRVLPLYKALTRQKI 119 TYY ++YG G++YY LG H Y SWW++LL+ IG SLVICSLDRV+PLY+AL Q + Sbjct: 96 DTYYEQQYGIIGQLYYLLGFHHLYGSWWYMLLIASIGISLVICSLDRVIPLYRALKNQGV 155 Query: 120 RKHRQFLTRQKVALVAELKEEPEAWVARMVEPLQKKGYRVRTDGGALLAEKHRFSRWGPY 179 R++ FL RQ++ E++ +A + L+KK YRVR G++LAEK RFSRWGPY Sbjct: 156 RRNPVFLKRQRLYSRTEIELSGDA-ADSIRTRLKKKHYRVREQEGSILAEKGRFSRWGPY 214 Query: 180 VIHIGLIIFLLAVLARGLPGLNMDQHLAFPQGDTVRIP--DTTYYLKNEKFTVEFYTEEE 237 V HIGLIIFL+ + R +PG+ +D+ L +G+T +IP D YYLKN +FT++ Y ++ Sbjct: 215 VNHIGLIIFLIGAMLRFVPGMYVDETLWVREGETAQIPGTDGKYYLKNNRFTMQTYNKDT 274 Query: 238 MPKEF------RGTKILPKLYETKAVLYECTADCTDPSKEPQLAEVARHNIQVNSPLSYN 291 K F G + K + T AVLY+ D K P+L V +NI+VN PL ++ Sbjct: 275 EKKVFADAIDRAGNGKVAKSFTTDAVLYKREGDIVFGEK-PKLKRVKENNIRVNEPLRFD 333 Query: 292 GLKAYQFDYDLTPVLRSVQPELINAKSGEVYGKFKLDMNNPKRTFQAGP-YTLSLKEKYM 350 YQ DY + + V +LI+ K+ + +GK K+++ +P + G Y + + Sbjct: 334 SYSVYQVDYKENQLDQMV-FQLIDQKTKQSFGKLKINLLDPDTDYDLGNGYKVQIASYLP 392 Query: 351 DFGLNEEGQPVSKSPYPNAPAFLFLITGPGLPAGGQQYFYFPKQVDKEKFQMTAINDKLG 410 DF N++G+P +K+ PN PAF+F IT P P G + + + V+ + + Sbjct: 393 DFYFNKDGEPSTKTKVPNNPAFVFKITAPDKPKGEKSFVAIQQTVEGSESNKYKL----- 447 Query: 411 GSGRFLELEVGDMSDVDFSESTTYLNIRVDRAMPFVWIGAGIVMLGLILGFYWQHRRIWL 470 +F +E +++ T L +R D + + +G I M+G+I G YWQHRRIW+ Sbjct: 448 ---KFDHVE---------TKNITGLTVRKDLTLWVLAVGGAIFMIGVIQGMYWQHRRIWI 495 Query: 471 AANHGELVLGGHTNKNWFGFRREIVSILAKTDLT--VDEKSL 510 E+++ GHTNKNWFG +++I +L++T + D+K L Sbjct: 496 KTGDHEVLVAGHTNKNWFGLKKDIEFVLSETGIQEPADQKEL 537 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,319,017,653 Number of extensions: 147633733 Number of successful extensions: 349846 Number of sequences better than 1.0e-05: 355 Number of HSP's gapped: 348974 Number of HSP's successfully gapped: 357 Length of query: 517 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 377 Effective length of database: 1,741,754,103 Effective search space: 656641296831 Effective search space used: 656641296831 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)