BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0015.p (482 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9ZNW7.1 B9ZNW7_9GAMM Glycerol kinase 566 e-159 Q9X1E4.2 GLPK2_THEMA Glycerol kinase 2 EC=2.7.1.30 557 e-157 B1LBG8.1 B1LBG8_THESQ Glycerol kinase 556 e-156 B7RB01.1 B7RB01_9THEM Glycerol kinase EC=2.7.1.30 555 e-156 A5IMF5.1 A5IMF5_THEP1 Glycerol kinase 555 e-156 >B9ZNW7.1 B9ZNW7_9GAMM Glycerol kinase Length = 505 Score = 566 bits (1458), Expect = e-159, Method: Compositional matrix adjust. Identities = 275/480 (57%), Positives = 339/480 (70%), Gaps = 1/480 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 LFD++A ++ Q E Q +P PGWVEHD EIW TQ+ AAR + G A+++AIGI Sbjct: 20 LFDRDAAIIGVAQREFTQHYPEPGWVEHDAMEIWGTQIGAAREVLETRGIKPAQLAAIGI 79 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWD+ TGKPI+PAIVWQDRRTA C++LK RGL + I ++TGLVVDAYFS T Sbjct: 80 TNQRETTVVWDRETGKPIHPAIVWQDRRTASFCDELKARGLEEMIRSRTGLVVDAYFSGT 139 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 KL W+LDHV GAR RAE+GELL GT+D WLIW LT G H TDV+NASRT L+N+HE W Sbjct: 140 KLRWLLDHVDGARERAERGELLFGTMDTWLIWNLTRGERHVTDVSNASRTQLFNIHELDW 199 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGV-EIPIRSVLGDQQAALFGHTCLEAG 239 D +IPRA+LPEVR S G G+E FG EIPI V GDQ AALFG TC G Sbjct: 200 DDTLLDALAIPRAMLPEVRASSEVLGHTGTEMFGASEIPIAGVAGDQHAALFGQTCFGRG 259 Query: 240 SAKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEG 299 AKNTYGTGCF+LMNTGT+ VAS GLLTTVAW + ++ YALEGS+F+AGAA+QWL + Sbjct: 260 MAKNTYGTGCFLLMNTGTKPVASHSGLLTTVAWSVDGQVEYALEGSIFIAGAAIQWLRDE 319 Query: 300 LGVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRA 359 L +I+ DSE A PAF GLGAPYWDMYARGA+FGLTRGT H+ RA Sbjct: 320 LKLIDSAEDSEYYASKVEDSDGVYVVPAFAGLGAPYWDMYARGAIFGLTRGTRKEHITRA 379 Query: 360 TLESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXX 419 TL+SLA+Q++DVI AME+D+G+ L GLRVDGGAV N+++MQFQAD+LG V R + E Sbjct: 380 TLDSLAYQTKDVIDAMEQDSGIALKGLRVDGGAVANNVMMQFQADMLGVNVERPVVTEST 439 Query: 420 XXXXXXXXXXXSGIWNREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKHE 479 G W R++LE ++ +++F P M+A R+R Y W+ AV RTMGW + + Sbjct: 440 ALGAAYLAGLAVGFWTRDELEQKSQLDRVFEPQMDAATRDRYYRGWKKAVKRTMGWARED 499 >Q9X1E4.2 GLPK2_THEMA Glycerol kinase 2 EC=2.7.1.30 Length = 496 Score = 557 bits (1436), Expect = e-157, Method: Compositional matrix adjust. Identities = 265/479 (55%), Positives = 339/479 (70%), Gaps = 1/479 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ +VS+ E RQ +PRPGWVEHDP EIWE+Q+ A+ AI E+G +I+AIGI Sbjct: 18 VFDEKGNVVSKVNKEFRQIYPRPGWVEHDPVEIWESQIEVAKKAIEEAGIKPEDIAAIGI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C++LK +G ++ I +TGLV+DAYFS T Sbjct: 78 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDELKEKGYSEFIRERTGLVIDAYFSGT 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 138 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGRVHVTDYSNASRTMIFNIHKLDW 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPRA+LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 198 DDEILELLNIPRAMLPQVMPSSHVYGYTAKDIFGVEIPIAGDAGDQQAALFGQACFQPGM 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 258 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 318 KIISNAAETEELATKVPDNGGVFFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q+RDV+ MEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 378 LESIAYQTRDVVEVMEKDSDIKVETLRVDGGAVVNNFLMQFQADILGVPVERPVVNETTA 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W ++E++ S + ++ F PSM +EERER Y W++AVSR++GWEK Sbjct: 438 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLYSKWKEAVSRSLGWEKR 496 >B1LBG8.1 B1LBG8_THESQ Glycerol kinase Length = 496 Score = 556 bits (1433), Expect = e-156, Method: Compositional matrix adjust. Identities = 265/479 (55%), Positives = 338/479 (70%), Gaps = 1/479 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ ++S+ E +Q +PRPGWVEHDP EIWE+Q+ A AI E+G EI+AIGI Sbjct: 18 VFDEKGNVISKVNKEFKQIYPRPGWVEHDPMEIWESQIEVAERAIEEAGIKPEEIAAIGI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C++LK +G A+ I +TGLV+DAYFS T Sbjct: 78 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDELKEKGYAEFIRERTGLVIDAYFSGT 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 138 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGKVHVTDYSNASRTMIFNIHKLDW 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPRA+LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 198 DDEILELLNIPRAMLPQVMPSSHVYGYTAKDIFGVEIPIAGDAGDQQAALFGQACFQPGM 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 258 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 318 RIISNAAETEELATKVPDNGGVYFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q+RDV+ MEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 378 LESIAYQTRDVVEVMEKDSDIKVETLRVDGGAVVNNFLMQFQADILGIPVERPVVNETTA 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W ++E++ S + ++ F PSM +EERER Y W++AVSR++GWEK Sbjct: 438 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLYSKWKEAVSRSLGWEKR 496 >B7RB01.1 B7RB01_9THEM Glycerol kinase EC=2.7.1.30 Length = 503 Score = 555 bits (1431), Expect = e-156, Method: Compositional matrix adjust. Identities = 264/479 (55%), Positives = 338/479 (70%), Gaps = 1/479 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ +VS+ E RQ +P+PGWVEHDP EIWE+Q+ A+ AI E+G +I+AIGI Sbjct: 25 VFDEKGNVVSKVNKEFRQIYPKPGWVEHDPMEIWESQIEVAKKAIEEAGIKPQDIAAIGI 84 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C+ LK RG ++ I +TGLV+DAYFS T Sbjct: 85 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDDLKERGYSEFIRERTGLVIDAYFSGT 144 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 145 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGKVHVTDYSNASRTMIFNIHKLDW 204 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPR +LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 205 DDDILELLNIPRPMLPQVMPSSHVYGYTAKDIFGVEIPIAGNAGDQQAALFGQACFQPGM 264 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 265 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 324 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 325 RIISNAAETEELATKVPDNGGVFFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 384 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q++DV+ AMEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 385 LESIAYQTKDVVEAMEKDSDIKVDTLRVDGGAVVNNFLMQFQADILGVPVERPVVNETTA 444 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W ++E++ S + ++ F PSM +EERER Y W++AVSR++GWEK Sbjct: 445 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLYSKWKEAVSRSLGWEKQ 503 >A5IMF5.1 A5IMF5_THEP1 Glycerol kinase Length = 496 Score = 555 bits (1430), Expect = e-156, Method: Compositional matrix adjust. Identities = 264/479 (55%), Positives = 338/479 (70%), Gaps = 1/479 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ +VS+ E RQ +P+PGWVEHDP EIWE+Q+ A+ AI E+G +I+AIGI Sbjct: 18 VFDEKGNVVSKVNKEFRQIYPKPGWVEHDPMEIWESQIEVAKKAIEEAGIKPQDIAAIGI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C+ LK RG ++ I +TGLV+DAYFS T Sbjct: 78 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDDLKERGYSEFIRERTGLVIDAYFSGT 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 138 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGKVHVTDYSNASRTMIFNIHKLDW 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPR +LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 198 DDDILELLNIPRPMLPQVMPSSHVYGYTAKDIFGVEIPIAGNAGDQQAALFGQACFQPGM 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 258 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 318 RIISNAAETEELATKVPDNGGVFFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q++DV+ AMEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 378 LESIAYQTKDVVEAMEKDSDIKVDTLRVDGGAVVNNFLMQFQADILGVPVERPVVNETTA 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W ++E++ S + ++ F PSM +EERER Y W++AVSR++GWEK Sbjct: 438 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLYSKWKEAVSRSLGWEKQ 496 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,771,645,246 Number of extensions: 108080620 Number of successful extensions: 268296 Number of sequences better than 1.0e-05: 3267 Number of HSP's gapped: 261038 Number of HSP's successfully gapped: 3318 Length of query: 482 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 342 Effective length of database: 1,741,754,103 Effective search space: 595679903226 Effective search space used: 595679903226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)