BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0011.p (510 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6CP74.1 A6CP74_9BACI Acetolactate synthase large subunit 407 e-111 A6LX01.1 A6LX01_CLOB8 Thiamine pyrophosphate protein, central re... 396 e-108 Q5WGG1.1 Q5WGG1_BACSK Acetolactate synthase large subunit EC=2.2... 377 e-102 A9A4J0.1 A9A4J0_NITMS Acetolactate synthase EC=2.2.1.6 241 1e-61 B3TCK9.1 B3TCK9_9ARCH Putative Thiamine pyrophosphate enzyme, N-... 240 3e-61 >A6CP74.1 A6CP74_9BACI Acetolactate synthase large subunit Length = 549 Score = 407 bits (1047), Expect = e-111, Method: Compositional matrix adjust. Identities = 225/512 (43%), Positives = 312/512 (60%), Gaps = 10/512 (1%) Query: 6 IEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGH 65 I F+ HE +G+EA+GYAL LGVA GT+GPGGTNLLTAA AKA +LPVLFITGH Sbjct: 41 INFVLSSHEGGAGYEASGYALSKNTLGVAIGTTGPGGTNLLTAAGQAKAYHLPVLFITGH 100 Query: 66 QSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPV 125 Q I+ G Q QDSS++ DL MF P TL+S VER + F HAI A++G++GPV Sbjct: 101 QPIQSTGKAQGQDSSSFGTDLTKMFEPVTLFSARVERSEMFPLYLRHAIEKAVTGKKGPV 160 Query: 126 HLCIPFDVQTEVLEECRIVIPEREHLVSAGNLERVLSAINESSHPLIIAGKGVNRSGAHE 185 HL IP+DV E +E +P V + ++++V S +N + P++ GKGV S A+E Sbjct: 161 HLSIPYDVYMEEIESFYYPLPADIGTVISPDVDQVPSILNNAKKPVLFLGKGVGISNAYE 220 Query: 186 ELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGC-KHGEDLLNRSDVFIVLGSRL 244 E+ +AE +NIPV+T+P GKGA HPL G G+GG K E L + DV IV+G++L Sbjct: 221 EVGLVAEHWNIPVITTPCGKGAFPSVHPLSLGGFGLGGTEKSAEYLKSGIDVMIVVGTKL 280 Query: 245 SDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNFDSAAIKK 304 SDM++ A+ HP ++QFD D TF GK L T + GD + NL + + A + Sbjct: 281 SDMSLAGFSADMHPDLILQFDCDLTFAGKSLPVPTKVLLGDAKANLK---KLIEIAGCQP 337 Query: 305 HETETDVHYAEELPVLPK------LSLASVMDALSDLIPYNNTIFVDDGSHGFNAAKWYK 358 + +V L P L+ + L +P +F DDGSH F A + Y Sbjct: 338 NPALAEVAATSSLAKEPSAGRDGMLTSEQAVKTLRSHLPKEAILFGDDGSHTFYAVRHYD 397 Query: 359 VKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINTAVCKDIP 418 + +PG+ FD F MG++IG +IGAK+ASPE+ ++C+TGDGC +M GTE TA +P Sbjct: 398 IYQPGTFFFDDVFGAMGHAIGFSIGAKIASPEKPVVCLTGDGCTLMHGTEFITAKNNRVP 457 Query: 419 VIFIVVNNMQLDMALKGMEKTTGRIDGTIFEVPMDAVKFAESLGATGYRCETLEQFTSAI 478 V+F+V+NN QLDM KGM +TGR GT ++ P++A KFAE++G T +RC E+ AI Sbjct: 458 VLFVVLNNGQLDMVDKGMSFSTGRSVGTTYQTPLNAAKFAEAMGVTSFRCHDEEELAEAI 517 Query: 479 HAAVASNRVAVIELLTDRTEVPPTAHRTLNLN 510 +++ ++ VIE + D EVPPT R + L Sbjct: 518 QSSLQADGPVVIEAIVDPAEVPPTLSRVVTLK 549 >A6LX01.1 A6LX01_CLOB8 Thiamine pyrophosphate protein, central region Length = 543 Score = 396 bits (1018), Expect = e-108, Method: Compositional matrix adjust. Identities = 216/506 (42%), Positives = 309/506 (61%), Gaps = 9/506 (1%) Query: 6 IEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGH 65 I FI +HES +G+ AAGY+L KLGVA TSGPGGTN LTAA AKA ++PVLFITG Sbjct: 40 INFILSKHESGAGYAAAGYSLINKKLGVALVTSGPGGTNALTAAGQAKAYHVPVLFITGQ 99 Query: 66 QSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPV 125 QS+ +G QDS+ + DL MF T +S VER D F + HA+ A SG +GPV Sbjct: 100 QSMHNVGKAIGQDSTIFGTDLVRMFESVTKFSARVERADQFQILLKHALEKAYSGAKGPV 159 Query: 126 HLCIPFDVQTEVLEECRIVIPEREHLVSAGNLERVLSAINESSHPLIIAGKGVNRSGAHE 185 HL IP D+ E ++E + +P + + L+ +S +N + P+I+AGKGV+ S A+E Sbjct: 160 HLSIPLDILDEEIDEFELDLPNNMDKLISSRLDEFVSKLNNARKPVILAGKGVHSSLAYE 219 Query: 186 ELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGC-KHGEDLLNRSDVFIVLGSRL 244 E+ +AE ++IPV+T+PGGKG + +HPL G +G+GG K E + N +D+ IV+GS+L Sbjct: 220 EVKNIAEIWDIPVMTTPGGKGTFSENHPLSLGALGLGGTDKSSEYIKNDTDLMIVIGSKL 279 Query: 245 SDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNFDSAAIKK 304 SDM++ + +N+PK ++ D D TF+ K L +T+ + GD++ NL L D A K+ Sbjct: 280 SDMSLVGISPDNYPKEVVHLDYDNTFIEKALPVKTLPIIGDIKANLHLVL---DKAKDKR 336 Query: 305 HETETDVHYAEELPVLPK-----LSLASVMDALSDLIPYNNTIFVDDGSHGFNAAKWYKV 359 + T + E K LS A M+ + ++P + +F DDGSH F A +++ + Sbjct: 337 RKEYTLLEEIYEFKNNIKENNKYLSSAHAMEIIRKVLPDDAVVFGDDGSHSFYAIRYFNI 396 Query: 360 KKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINTAVCKDIPV 419 +KPG+ FD F MG++IG +IGA++A+ I+CITGDGC M G EI+TA +IPV Sbjct: 397 RKPGTFFFDDVFGAMGHAIGYSIGAQLANYNSRIVCITGDGCTFMQGAEISTAANYNIPV 456 Query: 420 IFIVVNNMQLDMALKGMEKTTGRIDGTIFEVPMDAVKFAESLGATGYRCETLEQFTSAIH 479 FIV+NN +LDM K M K G+ GT + VP+D KF ESLG YRC + A+ Sbjct: 457 TFIVINNGRLDMPEKAMIKFFGKTVGTNYNVPLDGKKFGESLGVKSYRCCNELELKEALE 516 Query: 480 AAVASNRVAVIELLTDRTEVPPTAHR 505 + +IE++ D E+PPT R Sbjct: 517 ISNLHKEAILIEVMVDPNEIPPTMKR 542 >Q5WGG1.1 Q5WGG1_BACSK Acetolactate synthase large subunit EC=2.2.1.6 Length = 544 Score = 377 bits (968), Expect = e-102, Method: Compositional matrix adjust. Identities = 213/512 (41%), Positives = 305/512 (59%), Gaps = 10/512 (1%) Query: 1 MVDYGIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVL 60 M + G+ F+ RHE +GF A GYAL+TG LGVA GTSGPGGTN++TAAA A+A + PVL Sbjct: 35 MEESGVSFVLARHEGGAGFMATGYALQTGGLGVAIGTSGPGGTNMITAAAQAQAFHAPVL 94 Query: 61 FITGHQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSG 120 FITGH + + G+ QDSS + DL +F+P T +S VE HA AL+G Sbjct: 95 FITGHPPLSQSGMALGQDSSMFGTDLVALFKPVTKFSAKVESKGMLKRYLQHAEEQALTG 154 Query: 121 QRGPVHLCIPFDVQTEVLEECRIV-IPEREHLVSAGNLERVLSAINESSHPLIIAGKGVN 179 +GPVHL +P D+ E ++E + +P ++H+ S GN++ + A+N + PL++ GKG + Sbjct: 155 AKGPVHLSVPLDILIEEVDEFTLSRLPVQDHIRS-GNIQLAIKALNRAKRPLMLVGKGAH 213 Query: 180 RSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGV----GGCKHGEDLLNRSD 235 SGA++E+ Q+AE +++PVVT+PGGKG DHPL G +G+ G + +D D Sbjct: 214 FSGAYKEVEQVAEKWSLPVVTTPGGKGTFVTDHPLSLGSLGLGGTEGAAAYVKD--KPID 271 Query: 236 VFIVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQ 295 + +V+GS+LSDM+ L A P ++ FD FVGK L ++T+ V GD + NL L Sbjct: 272 LLLVVGSKLSDMSTVGLTAGGMPSQVLHFDYQGLFVGKSLPADTLFVQGDAKINLQQLLL 331 Query: 296 NFDSAAIKKHETETDVHYAEELPV--LPKLSLASVMDALSDLIPYNNTIFVDDGSHGFNA 353 ++ + PV +LS A M AL +++P + ++ DDGSH F A Sbjct: 332 EAKEFPQRQQVDLREYRDRNRAPVDGCDRLSTAQTMGALREVLPADTVVYGDDGSHTFYA 391 Query: 354 AKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINTAV 413 K ++ PG+ FD F MG++IG++IGAK+A P+ I C TGDGC M GTEI TAV Sbjct: 392 IKHFRTLVPGTFYFDDVFGAMGHAIGLSIGAKMAQPQRPIACFTGDGCLYMHGTEIATAV 451 Query: 414 CKDIPVIFIVVNNMQLDMALKGMEKTTGRIDGTIFEVPMDAVKFAESLGATGYRCETLEQ 473 + V+F+V NN LDM KGM+ GR GT +E ++A FAESLGA G+RC T+ + Sbjct: 452 DQKTNVLFVVFNNGMLDMVDKGMKHNLGRTAGTRYEQEINAALFAESLGAKGFRCNTVAE 511 Query: 474 FTSAIHAAVASNRVAVIELLTDRTEVPPTAHR 505 A+ V+E++ + E PPT R Sbjct: 512 VKEAVQKGNRFEGPVVVEVMVSKEETPPTLGR 543 >A9A4J0.1 A9A4J0_NITMS Acetolactate synthase EC=2.2.1.6 Length = 566 Score = 241 bits (615), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 170/520 (32%), Positives = 253/520 (48%), Gaps = 44/520 (8%) Query: 6 IEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGH 65 I I RHE S+ A G+ + K GV F TSGPG TN+LT A A+A++ P++ +TG Sbjct: 45 IRHILARHEQSAAHMADGFGRVSRKPGVCFATSGPGATNILTGIATAQADSAPMVAVTGQ 104 Query: 66 QSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPV 125 +G Q+S D+ M P Y+ + IA +G+ GPV Sbjct: 105 VPTPMIGKDAFQES-----DIIGMANPVVKYAFQPRHASEIPEVVKKGFFIAETGRPGPV 159 Query: 126 HLCIPFDVQTEVLEECRIVIPER-----EHLVSAGNLERVLSAINESSH---PLIIAGKG 177 + IP DVQ E +V P+ H + ++ V AI+ H P+I+AG G Sbjct: 160 LIDIPKDVQQN---EAEMVFPDEFKIQGYHPWADPDIVAVEKAIDMLLHAQKPVILAGGG 216 Query: 178 VNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVF 237 S A EL +AE +PVVT+ GKGA +HPL GPIG+ G ++ +D Sbjct: 217 TIISSAFAELQAVAEALMLPVVTTFKGKGAFPENHPLSLGPIGMHGHAEANKIMTEADCV 276 Query: 238 IVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNF 297 + +G+R SD ++ + +I DVDP +GK T++ +AV GD++ NL ++ Sbjct: 277 LAIGTRFSDRSVGTFEEFEKNLKIIHMDVDPAEIGKNQTAQ-LAVVGDVQMNLRIMVKLL 335 Query: 298 DSAAIK--------KHETETDVHYAEELPVLP-KLSLASVMDALSDLIPYNNTIFVDDGS 348 AIK KH ET ++ E L + P ++ A ++ L +++P + I + G Sbjct: 336 LQKAIKKTDETPWVKHVKETKAYWQENLKLHPGEMGAAKILRKLREILPKESIITTEVGQ 395 Query: 349 HGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTE 408 H A+ +Y V +PG+ MG AIGAKVA P+ ++ I GDG F M Sbjct: 396 HQMWASLFYDVIQPGTFFSSTGLGTMGWGFPAAIGAKVAKPDVPVVDIAGDGSFAMTENS 455 Query: 409 INTAVCKDIPVIFIVVNNMQLDMALKGMEKTTGRIDGTIFEVPM----------DAVKFA 458 + TAV +DIPVI V+NN L M + T +E M D VK A Sbjct: 456 LATAVLEDIPVIVFVMNNFTLGM--------VAQWQRTFYERRMIGVDQGKWCPDYVKLA 507 Query: 459 ESLGATGYRCETLEQFTSAIHAAVASNRVAVIELLTDRTE 498 ES GA G R +++++ AI A++S+ VI++ D E Sbjct: 508 ESYGAQGIRAQSMDELDKAIKDALSSDVATVIDIPIDPEE 547 >B3TCK9.1 B3TCK9_9ARCH Putative Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain protein Length = 566 Score = 240 bits (612), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 164/506 (32%), Positives = 251/506 (49%), Gaps = 29/506 (5%) Query: 12 RHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGHQSIKEL 71 RHE S+ A G+ + K GV F TSGPG TNLLT A A+A++ P++ ITG +K + Sbjct: 52 RHEQSAAHMADGFGRVSRKPGVCFATSGPGSTNLLTGIATAQADSAPMVAITGQVPVKMI 111 Query: 72 GIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPVHLCIPF 131 G Q+S D+ M P Y+ + + IA +G+ GPV L IP Sbjct: 112 GRDAFQES-----DIIGMSNPCVKYAFQPRTPEEIPEMVKKGFYIAETGRPGPVLLDIPK 166 Query: 132 DVQTEVLEECRIVIPEREHL--------VSAGNLERVLSAINESSHPLIIAGKGVNRSGA 183 DVQ E I PE + + ++ER ++ + S P+I+AG G S A Sbjct: 167 DVQQN---ESEITFPEEVRIPGYHPWSSIDITDIERAINLLLSSEKPIILAGGGTIISSA 223 Query: 184 HEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVFIVLGSR 243 EL +AE IPVVT+ GKG+ +HPL GPIG+ G ++ D + +G+R Sbjct: 224 FAELQSVAEMLMIPVVTTFKGKGSFPENHPLSLGPIGMHGHAEANKIMTEVDCVLAIGTR 283 Query: 244 LSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNFDSAAIK 303 SD ++ + +I DVDP +GK T++ +AV GD++ +L L+ A K Sbjct: 284 FSDRSVGTFEEFERNLKIIHMDVDPAEIGKNQTAQ-VAVVGDVKASLRIMLKLLVKKAFK 342 Query: 304 --------KHETETDVHYAEELPVLP-KLSLASVMDALSDLIPYNNTIFVDDGSHGFNAA 354 KH E ++ L + P ++S A ++ L +++P + + + G H A+ Sbjct: 343 KSEDNKWLKHVNEVKDYWRANLKMHPGEMSAARILRKLREVLPKESIVTTEVGQHQMWAS 402 Query: 355 KWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINTAVC 414 ++ V PG+ MG +IGAKVA P+ +L I G+G F M + T+V Sbjct: 403 LFFDVIHPGTFFSSTGLGTMGWGFPASIGAKVARPDVPVLDIAGEGSFNMTENSLATSVL 462 Query: 415 KDIPVIFIVVNNMQLDMALKGMEKT--TGRIDGTIFEVPMDAVKFAESLGATGYRCETLE 472 +IPVI +VNN L M + ++T R+ G + D VK AES GA G R ++L+ Sbjct: 463 DNIPVIVFLVNNFSLGMVAQ-WQRTFYERRMIGIDLKNCPDYVKLAESYGAQGIRAQSLD 521 Query: 473 QFTSAIHAAVASNRVAVIELLTDRTE 498 + AI ++S+ VI++ D E Sbjct: 522 ELEKAIKTGLSSDVTTVIDIPIDPEE 547 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,088,839,852 Number of extensions: 131029682 Number of successful extensions: 332895 Number of sequences better than 1.0e-05: 5298 Number of HSP's gapped: 319010 Number of HSP's successfully gapped: 5792 Length of query: 510 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 370 Effective length of database: 1,741,754,103 Effective search space: 644449018110 Effective search space used: 644449018110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)