BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0009.p (416 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGS5.1 B3KGS5_9BACI Chemotaxis sensory transducer 504 e-141 B1DJ56.1 B1DJ56_9BACL Methyl-accepting chemotaxis sensory transd... 431 e-119 B7GLS1.1 B7GLS1_ANOFW Methyl-accepting chemotaxis protein 157 2e-36 B4AFE5.1 B4AFE5_BACPU Sensor histidine kinase 156 3e-36 Q2RIE3.1 Q2RIE3_MOOTA Methyl-accepting chemotaxis sensory transd... 155 9e-36 >B3KGS5.1 B3KGS5_9BACI Chemotaxis sensory transducer Length = 416 Score = 504 bits (1297), Expect = e-141, Method: Compositional matrix adjust. Identities = 257/416 (61%), Positives = 319/416 (76%) Query: 1 MDWMNKLPLKQRIVAGCYLIAALFAVPVLITLLIMGKXXXXXXXXXXXXXXTFPLSRFIE 60 M W N+LP+ Q+I A CYLIAALFAVP+L+ ++MGK TFPLSR IE Sbjct: 1 MAWFNRLPMSQKITAACYLIAALFAVPMLLAFILMGKAIIGVVIVVVLTGLTFPLSRLIE 60 Query: 61 RTLTSSFDDISNVTHTIAKGDFTNRADENGSMGDISRSFNTMIDKLKKILTDASQITRQV 120 +TLT+SF +IS+V+ IAKGDFT + +E G M D+SR+FN+M+DKL+KIL + S ITRQV Sbjct: 61 KTLTNSFAEISSVSAKIAKGDFTFQVEEVGGMNDLSRTFNSMVDKLRKILQETSDITRQV 120 Query: 121 MDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTESIKDIETKVSNYTSST 180 M +S I KNQ L VM QV+ SSNELA+GANEIS D+++MT SI++IE KVSNYT ST Sbjct: 121 MTSSSSISIKNQELIQVMNQVSLSSNELAVGANEISEDVSEMTHSIREIEEKVSNYTHST 180 Query: 181 KEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALSQNARGITMITKTIT 240 +EMN RS+ TL LVE+GR SV Q+ GM++NI+AT KV+++I+AL+Q+ARGIT IT+TI+ Sbjct: 181 QEMNTRSIETLGLVEQGRDSVAKQSAGMQRNIEATAKVSESIDALAQSARGITKITETIS 240 Query: 241 EIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTKEVFSLVRSIENDIK 300 E+A+QTNLLSLNASIEAARAGEHG GFAVVAQEVRKLAEEST+ST+EVF+LVRSIE+DIK Sbjct: 241 ELADQTNLLSLNASIEAARAGEHGAGFAVVAQEVRKLAEESTSSTREVFNLVRSIESDIK 300 Query: 301 QAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASFSAESDLMLESALKISG 360 A +NI INEEVV+ Q EMI E +F +IV SVQYI+EQIA+FS ES ML+SA IS Sbjct: 301 HASQNIKINEEVVRQQTEMIREAELVFMEIVNSVQYISEQIAAFSKESQSMLDSAQSISS 360 Query: 361 AIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMTQAVFQLQKTIHIFKF 416 AI+NIS MNE I ++Q +AEE E M AVF+LQKTI+IFKF Sbjct: 361 AIQNISAITQQSAAGTEQVSASMNEGISSIQKMAEEAEAMNNAVFRLQKTINIFKF 416 >B1DJ56.1 B1DJ56_9BACL Methyl-accepting chemotaxis sensory transducer Length = 421 Score = 431 bits (1109), Expect = e-119, Method: Compositional matrix adjust. Identities = 224/416 (53%), Positives = 296/416 (71%), Gaps = 3/416 (0%) Query: 3 WMNKLPLKQRIVAGCYLIAALFAVPV-LITLLIMGKXXXXXXXXXXXXXXTFPLSRFIER 61 W L ++ +++ Y L A+ + L+ L G +P FIE+ Sbjct: 4 WYYSLSVRSKLMTSIYFNLVLVAIALNLVILFSGGSILAGLIVTVVLAILAYPTVGFIEK 63 Query: 62 TLTSSFDDISNVTHTIAKGDFTNRAD--ENGSMGDISRSFNTMIDKLKKILTDASQITRQ 119 L SSF+++SN I+KGDFT R D N S+G++ +FN+M+DKL+ ILT+ S ITR Sbjct: 64 ALNSSFEEMSNAAFRISKGDFTQRIDTAANASLGEVGHAFNSMVDKLRDILTETSNITRH 123 Query: 120 VMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTESIKDIETKVSNYTSS 179 V + SR I DKN +++I M QVA+SSNELA GANEIS D+ADM+ESI +IE KV+NY + Sbjct: 124 VSETSRNIFDKNNHIQIAMEQVAASSNELATGANEISEDVADMSESIAEIEKKVANYAHA 183 Query: 180 TKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALSQNARGITMITKTI 239 TKEMN RS TL LV+KGR +++ Q+EGMR+N++AT+KV++TI L++ A+GIT +T I Sbjct: 184 TKEMNERSEFTLGLVDKGRGALEAQSEGMRRNVEATEKVSETITDLARKAQGITAMTTAI 243 Query: 240 TEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTKEVFSLVRSIENDI 299 ++IAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEE+T STKEVFSLV+SI+ I Sbjct: 244 SDIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEEATVSTKEVFSLVKSIDQGI 303 Query: 300 KQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASFSAESDLMLESALKIS 359 KQ I ++ +NEEVV++Q E I E+ +FA+IVQSVQ+IT+QIA+F++ESD MLESA KIS Sbjct: 304 KQTINHMKVNEEVVKIQTERIRESEMVFAEIVQSVQFITQQIAAFASESDQMLESARKIS 363 Query: 360 GAIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMTQAVFQLQKTIHIFK 415 AI+NIS MNEQI ++Q V +ETE+M QLQ+TI +FK Sbjct: 364 DAIQNISAITEESAAGTQQVSASMNEQISSVQGVVQETERMLNMSTQLQRTIQVFK 419 >B7GLS1.1 B7GLS1_ANOFW Methyl-accepting chemotaxis protein Length = 550 Score = 157 bits (398), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 113/362 (31%), Positives = 189/362 (52%), Gaps = 9/362 (2%) Query: 61 RTLTSSFDDISNVTHTIAKGDF------TNRADENGSMGDISRSFNTMIDKLKKILTDAS 114 R +T+ I+ IA G+ N DE + D++ +F TM D+L +L Sbjct: 191 RKITTPLKKIAENAKQIASGNLRIEPVKYNGKDE---LADLNAAFTTMADQLNGLLQSVD 247 Query: 115 QITRQVMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTESIKDIETKVS 174 +++QV ++ I+++ + L + QVA S+NEL+ GA IS D+ + I+ +E V Sbjct: 248 TVSKQVETFAKEIDEETKMLTEINNQVAVSTNELSAGAQSISEDLQSAVQLIERMEGTVQ 307 Query: 175 NYTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALSQNARGITM 234 + T++ R S +E + G+ ++ Q + +N +AT + + E + A I Sbjct: 308 QNVARTEQAARYSDEAVEAIVHGQAALAEQKTFIDENAKATASIEQSTEQFAAYASKIED 367 Query: 235 ITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTKEVFSLVRS 294 + K +++IA QTNLL+LNA+IEAARAGE G+GFAVVA+EVRKLAEES+ +T ++F +V+ Sbjct: 368 MAKAVSDIATQTNLLALNAAIEAARAGEAGKGFAVVAEEVRKLAEESSKATNQIFEMVQL 427 Query: 295 IENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASFSAESDLMLES 354 I++ + E + + + Q + I T FAQI VQ+IT + + D E Sbjct: 428 IQSGLTNVTEAVRRGVNIAEAQTKAIETTTEAFAQIESKVQHITTDMQALVIGMDESKEL 487 Query: 355 ALKISGAIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMTQAVFQLQKTIHIF 414 + +E+IS M EQ+ A + ++E+ + Q LQ+T+ F Sbjct: 488 GKNVLQNVESISAVVEQSAAGSEEIAASMVEQLEAFKKMSEKVTTLRQLTDDLQQTMAKF 547 Query: 415 KF 416 + Sbjct: 548 QM 549 >B4AFE5.1 B4AFE5_BACPU Sensor histidine kinase Length = 549 Score = 156 bits (395), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 107/362 (29%), Positives = 195/362 (53%), Gaps = 9/362 (2%) Query: 61 RTLTSSFDDISNVTHTIAKGDFT------NRADENGSMGDISRSFNTMIDKLKKILTDAS 114 R++ + +S IA+G+ R DE +G ++ SFN M D+L ++ + S Sbjct: 190 RSIVTPIKSLSYRAKQIAEGNLAVERMDIQRKDE---IGGLNESFNQMTDQLISLIKEIS 246 Query: 115 QITRQVMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTESIKDIETKVS 174 ++ QV S ++D+N+ L QV+ S++E+A G+ IS D+ I+ ++ Sbjct: 247 NVSSQVETFSIQLDDENKKLMESANQVSVSTDEMANGSQAISEDLQHGVSLIEKMDQHGR 306 Query: 175 NYTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALSQNARGITM 234 + ++ + + + +E VE G+ ++ + KN AT+++ + +Q A GI+ Sbjct: 307 KNSERSQTVIQSTGDAIEAVESGKHTLTETKTAIEKNTHATRQIEQSAGEFTQYASGISA 366 Query: 235 ITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTKEVFSLVRS 294 + KT+++IA+QTNLLSLNA+IEAARAGE G+GFAVVA E+RKLA+ES+ +T+++F +V Sbjct: 367 MAKTVSDIADQTNLLSLNAAIEAARAGEAGKGFAVVADEIRKLADESSNATRQIFDMVSH 426 Query: 295 IENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASFSAESDLMLES 354 IE I+ + + ++ Q + + +T++ F I Q+I ++A + + E Sbjct: 427 IERGIQSISQTVKEGVKLSLQQQDAMDKTSHSFEDIETKAQHIKREMAVLNDQIVQSTEL 486 Query: 355 ALKISGAIENISXXXXXXXXXXXXXXXXMNEQIHALQSVAEETEKMTQAVFQLQKTIHIF 414 ++ +IENIS NEQ+ + + ++ E++ +L +T+H F Sbjct: 487 GGQVLNSIENISAVVEETAAGSEEISASANEQLQSFHQMNKQVEELMSMTARLNETVHRF 546 Query: 415 KF 416 K Sbjct: 547 KL 548 >Q2RIE3.1 Q2RIE3_MOOTA Methyl-accepting chemotaxis sensory transducer Length = 659 Score = 155 bits (391), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 105/319 (32%), Positives = 181/319 (56%), Gaps = 14/319 (4%) Query: 53 FPLSRFIERTLTSSFDDISNVTHTIAKGDFTNRADEN--GSMGDISRSFNTMIDKLK--- 107 F +SR I R + + ++ +A G+ + N G +G + RS TM+ L+ Sbjct: 298 FYISRKIARPIIT----LAGQADVLATGNLQVDINTNFYGELGTLGRSLKTMVTNLRSIV 353 Query: 108 -KILTDASQITRQVMDASRGIEDKNQNLKIVMAQVASSSNELALGANEISVDIADMTESI 166 K+ A+QI + S E+ +++++ QVA++ ++A GAN+ + ++ E + Sbjct: 354 QKVQDSANQIASSAQEFSASTEEASRSVE----QVANAIQDMARGANDQATQSQNIAELV 409 Query: 167 KDIETKVSNYTSSTKEMNRRSVHTLELVEKGRQSVDTQAEGMRKNIQATQKVADTIEALS 226 +I + + + + R S T ELV+ G S++ Q + M +N+QA Q V++ I L+ Sbjct: 410 NNITGAIGSTRDRVEALARYSEQTGELVDDGLASMENQNDKMAENLQAAQAVSEAINKLA 469 Query: 227 QNARGITMITKTITEIAEQTNLLSLNASIEAARAGEHGRGFAVVAQEVRKLAEESTASTK 286 + AR + I +TIT IA+QTNLL+LNA+IEAARAGEHGRGFAVVA+EVRKLAE S + Sbjct: 470 RGAREVGQILETITSIADQTNLLALNAAIEAARAGEHGRGFAVVAEEVRKLAEGSAQAAS 529 Query: 287 EVFSLVRSIENDIKQAIENIAINEEVVQVQNEMIIETANIFAQIVQSVQYITEQIASFSA 346 E+ +V+ I+++ + A+ + + +V Q + + +F I Q V+ + + I +A Sbjct: 530 EIGQIVQKIQDEAQGAVAEMDKAKVIVDAQQDAVNHANEVFQNISQKVKAMVKGIEEIAA 589 Query: 347 ESDLMLESALKISGAIENI 365 ++ + A KI+ AI+ + Sbjct: 590 ATEQINNEARKITEAIQGV 608 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.126 0.323 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,649,500,450 Number of extensions: 52252712 Number of successful extensions: 324306 Number of sequences better than 1.0e-05: 12746 Number of HSP's gapped: 301011 Number of HSP's successfully gapped: 12869 Length of query: 416 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 278 Effective length of database: 1,760,596,133 Effective search space: 489445724974 Effective search space used: 489445724974 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 132 (55.5 bits)