BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0007.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65LG6.1 Q65LG6_BACLD Short-chain dehydrogenase/reductase SDR 336 2e-90 C5CKP2.1 C5CKP2_VARPD Short-chain dehydrogenase/reductase SDR 271 4e-71 Q7NI29.1 Q7NI29_GLOVI Glr2355 protein 262 3e-68 Q1D9A0.1 Q1D9A0_MYXXD Oxidoreductase, short chain dehydrogenase/... 219 2e-55 A0ADE2.1 A0ADE2_STRAM Putative oxidoreductase 214 5e-54 >Q65LG6.1 Q65LG6_BACLD Short-chain dehydrogenase/reductase SDR Length = 277 Score = 336 bits (861), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 161/279 (57%), Positives = 209/279 (74%), Gaps = 2/279 (0%) Query: 7 MTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRSRGLSQEDRRKGTIDSVLDEIR 66 M L+GK+ALVTGGSRGAGR IA EL KAGA VYITGRS + ++ GTID + +I Sbjct: 1 MKPLKGKIALVTGGSRGAGRAIAAELGKAGATVYITGRSIRGASTNQWPGTIDDTVSQIE 60 Query: 67 AAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPTDH 126 A+GG+ AVRCDHT D+ETEAVI +I EQG+LDIL+NNVWG ++ + +KPFWEL + Sbjct: 61 ASGGKAIAVRCDHTNDAETEAVIAKIREEQGKLDILINNVWGAHDLGVEAKPFWELSLKN 120 Query: 127 WSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTTYWDHYKYIDNFYYDLAKNALVRM 186 W MF AGVRAQL TN++AVPL+R N++ +IIHTT+WD KY FYYDLAKNA+VRM Sbjct: 121 WDTMFAAGVRAQLATNHFAVPLLRENKQ--ALIIHTTFWDENKYTGQFYYDLAKNAIVRM 178 Query: 187 AFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQDVAELQTSESTAYIGRAVVR 246 A+GLS+EL D IAVI +SPG+MRTELVL+ +DE HW++ +L+ +E+ Y+GR + Sbjct: 179 AYGLSLELKRDNIAVIAVSPGFMRTELVLKYHESDEAHWRESEDLRRTETPYYVGRGITA 238 Query: 247 LAADPEVLAMTGIPQQVGSLAEKYGFTDVDGRLVPVFKV 285 LA DP+V+ +G +VG LA++Y FTDVDGR +P F + Sbjct: 239 LAKDPDVMEKSGRALRVGDLAKEYQFTDVDGRYIPPFTI 277 >C5CKP2.1 C5CKP2_VARPD Short-chain dehydrogenase/reductase SDR Length = 298 Score = 271 bits (694), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 142/292 (48%), Positives = 190/292 (65%), Gaps = 17/292 (5%) Query: 9 NLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRS-RG-----------LSQEDRRKG 56 L+G+VA+VTG SRGAGRGIALEL AGA VY+TGRS RG LS+ + G Sbjct: 9 QLKGRVAVVTGASRGAGRGIALELGAAGATVYVTGRSTRGQPANTYGQLLALSELEALPG 68 Query: 57 TIDSVLDEIRAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNER--SI 114 +ID DE+ GG G AVRCDHT++ E + + ++ EQGR+D+LVNN WGG+E + Sbjct: 69 SIDETADEVTRLGGRGIAVRCDHTQEDEVKQLFAQVQREQGRVDLLVNNAWGGHETFTGV 128 Query: 115 ASKPFWELPTDHWSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTTYWDHYKYI-DN 173 PFWE P +W MFD GVR L+ + +A PLM + + G +I+ TT+WD Y+ N Sbjct: 129 FEAPFWEHPLSNWDSMFDRGVRNHLLASRFAAPLMVAQKSG--LIVTTTFWDRGNYLRGN 186 Query: 174 FYYDLAKNALVRMAFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQDVAELQT 233 +YDLAK+A+ R+AFG++ EL D +A + +SPGWMRTE VL + TDE HW++ L Sbjct: 187 LFYDLAKSAMTRLAFGMAQELRPDGVASLAVSPGWMRTEFVLAAHKTDEAHWREHPALAR 246 Query: 234 SESTAYIGRAVVRLAADPEVLAMTGIPQQVGSLAEKYGFTDVDGRLVPVFKV 285 +ES Y+GRAV LA D VL +G +V LA +YGFTD+DGR V F++ Sbjct: 247 TESPRYLGRAVAALAGDARVLEKSGGVHRVADLAREYGFTDIDGRQVEAFEL 298 >Q7NI29.1 Q7NI29_GLOVI Glr2355 protein Length = 282 Score = 262 bits (669), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 145/282 (51%), Positives = 185/282 (65%), Gaps = 12/282 (4%) Query: 7 MTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRS-RGLSQED-RRKGTIDSVLDE 64 M L G+VA+VTG SRGAGRGIAL L GA VY+TGR+ RG S GTI+ + Sbjct: 1 MGLLSGRVAVVTGASRGAGRGIALALGDTGATVYVTGRTVRGGSLPFIGAPGTIEDTAEG 60 Query: 65 IRAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPT 124 + A GG G AVRCDHT D ++ A+ R++ EQGRLD+LVNN WGGNE S A PFWE P Sbjct: 61 VTARGGRGIAVRCDHTDDIQSAALFERVAHEQGRLDLLVNNAWGGNEGSFAEVPFWEEPL 120 Query: 125 DHWSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTTYWDHYKYIDNFYYDLAKNALV 184 +W MF AGVRA L + +AVP+M + G G+I+ TT++D ++I N YYDLAK ++ Sbjct: 121 TNWDCMFTAGVRAHLAASRFAVPVML--KAGCGLIVCTTFYDEGRFIGNLYYDLAKASIN 178 Query: 185 RMAFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQDVAELQTSESTAYIGRAV 244 R+AFGL+ +L IA + LSPG+MRTELVL ++ D + V E EST YIGRAV Sbjct: 179 RLAFGLACQLRPHGIAAVALSPGFMRTELVL--MHPDPQ----VGE--PGESTEYIGRAV 230 Query: 245 VRLAADPEVLAMTGIPQQVGSLAEKYGFTDVDGRLVPVFKVP 286 LAADP +L +G VG LA +YG D+DG P F++P Sbjct: 231 AALAADPHILERSGRVLVVGELAREYGLCDIDGSQPPPFRLP 272 >Q1D9A0.1 Q1D9A0_MYXXD Oxidoreductase, short chain dehydrogenase/reductase family Length = 320 Score = 219 bits (559), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 124/276 (44%), Positives = 166/276 (60%), Gaps = 7/276 (2%) Query: 7 MTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRS-RGLSQEDRRKGTIDSVLDEI 65 M L+G+VALV G +RGAGRGIA L +AGA VY TGRS RG R TI+ + + Sbjct: 4 MKPLEGRVALVAGATRGAGRGIATMLGEAGATVYCTGRSVRGRPATGSRPETIEETAEIV 63 Query: 66 RAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPTD 125 A GG G AVR DH+ +SE EA+ RI EQGRLDILVN++WGG+ + FW+ Sbjct: 64 DAKGGRGIAVRVDHSVESEVEALCARIQREQGRLDILVNDIWGGDGLTEFGPAFWKQSAA 123 Query: 126 HWSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTTYWDHYKYIDNFYYDLAKNALVR 185 MF+ V L+T+ +AVPLM G+I+ T D + Y N +YDL K +++R Sbjct: 124 KGQQMFERAVFTHLLTSRHAVPLMLGGER--GLIVEITDGDSFGYRGNLWYDLVKMSVIR 181 Query: 186 MAFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQDVA----ELQTSESTAYIG 241 +AFG+S EL K+ + L+PG++R+E +LE+ E +W++ A SES Y+G Sbjct: 182 LAFGMSWELRRTKVTALALTPGFLRSEEMLENFGVTEANWREGAAKDPHFIASESPCYVG 241 Query: 242 RAVVRLAADPEVLAMTGIPQQVGSLAEKYGFTDVDG 277 RAV LAADP V A G +LA +YGFTD DG Sbjct: 242 RAVAALAADPNVGAKAGRVFSSWALAREYGFTDADG 277 >A0ADE2.1 A0ADE2_STRAM Putative oxidoreductase Length = 304 Score = 214 bits (546), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 119/283 (42%), Positives = 167/283 (59%), Gaps = 15/283 (5%) Query: 6 MMTNLQGKVALVTGGSRGAGRGIALELAKAGAYVYITGRS-RGLSQEDRRKGTIDSVLDE 64 M +L+GKVALV G +RGAGRGIA+EL AGA VY+TGRS R E R TI+ D Sbjct: 1 MSRSLEGKVALVAGATRGAGRGIAVELGAAGATVYVTGRSTRARRSEYDRPETIEDTADL 60 Query: 65 IRAAGGEGEAVRCDHTRDSETEAVIGRISSEQGRLDILVNNVWGGNERSIASKPFWELPT 124 + AGG G AV DH ++ ++ R++ EQ RLD+LVN++WGG P WE Sbjct: 61 VTEAGGHGIAVPTDHLDPAQVAVLVDRVAREQNRLDVLVNDIWGGENLFAWDSPVWEHDL 120 Query: 125 DHWSHMFDAGVRAQLMTNYYAVPLMRSNREGGGIIIHTT------YWDHYKYIDNFYYDL 178 D+ + V +T+++A+PL+ R GG+++ T DHY+ +F+YDL Sbjct: 121 DNGLRLLRLAVETHAVTSHHALPLLL--RHPGGLVVEVTDGTDDYNRDHYRV--SFFYDL 176 Query: 179 AKNALVRMAFGLSVELAADKIAVIPLSPGWMRTELVLESLNTDEEHWQD----VAELQTS 234 AK +++RMAF L EL + L+PGW+R+E++L+ EE+W+D V S Sbjct: 177 AKTSVLRMAFALGHELGPRGATAVALTPGWLRSEIMLDHFGVREENWRDALDRVPHFAIS 236 Query: 235 ESTAYIGRAVVRLAADPEVLAMTGIPQQVGSLAEKYGFTDVDG 277 E+ Y+GRAV LAADP+V G G LA++YGFTD+DG Sbjct: 237 ETPRYVGRAVTALAADPDVARFNGRSLSSGGLAQEYGFTDLDG 279 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,700,423,677 Number of extensions: 70076698 Number of successful extensions: 268652 Number of sequences better than 1.0e-05: 12411 Number of HSP's gapped: 262989 Number of HSP's successfully gapped: 12421 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)