BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0006.p (530 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL73.1 B3KL73_9BACI D-3-phosphoglycerate dehydrogenase 894 0.0 B1DJ88.1 B1DJ88_9BACL D-3-phosphoglycerate dehydrogenase EC=1.1.... 840 0.0 C0ZC90.1 C0ZC90_BREBN D-3-phosphoglycerate dehydrogenase EC=1.1.... 670 0.0 Q8EN61.1 Q8EN61_OCEIH Phosphoglycerate dehydrogenase EC=1.1.1.95 558 e-157 B7DRL0.1 B7DRL0_9BACL D-3-phosphoglycerate dehydrogenase EC=1.1.... 556 e-156 >B3KL73.1 B3KL73_9BACI D-3-phosphoglycerate dehydrogenase Length = 530 Score = 894 bits (2309), Expect = 0.0, Method: Compositional matrix adjust. Identities = 430/529 (81%), Positives = 484/529 (91%) Query: 1 MFKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKII 60 MFKVLVSDPISDLGIQQLMDA+DV VDKKTGLSEDELTAIIGEYDGLLVRSQT VTE+I+ Sbjct: 1 MFKVLVSDPISDLGIQQLMDAADVQVDKKTGLSEDELTAIIGEYDGLLVRSQTRVTERIM 60 Query: 61 AAGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQA 120 AGT LKV+GRAGVGVDNI LEAAT+ G+VVINAPDGNTITT EH FAMM+ALARHIPQA Sbjct: 61 NAGTNLKVVGRAGVGVDNIDLEAATKRGIVVINAPDGNTITTAEHTFAMMIALARHIPQA 120 Query: 121 YAKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAE 180 YAKT+ G WDRK+FLGVELR KTLGV+GMGRIGSEVAKRAKAFGMNILA+DPFLT +RA+ Sbjct: 121 YAKTVGGTWDRKSFLGVELRNKTLGVMGMGRIGSEVAKRAKAFGMNILAFDPFLTEERAD 180 Query: 181 KLEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMA 240 KL VKL+SVD+I+R ADFITVHTPLTPETRHMI+RPQFEVMK+GMRIVNCARGG+IDE A Sbjct: 181 KLGVKLSSVDNIIRNADFITVHTPLTPETRHMIARPQFEVMKRGMRIVNCARGGIIDERA 240 Query: 241 LVEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQV 300 LVEAID GIVAGAAFDVFE EPP++DHPFL+HPK+IVTPHLGASTVEAQENVAIDVSEQV Sbjct: 241 LVEAIDEGIVAGAAFDVFEHEPPESDHPFLNHPKVIVTPHLGASTVEAQENVAIDVSEQV 300 Query: 301 LHILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLS 360 LHILRNEPF NAVN P VA SVMNKLQPYF+LGEKLGS A+Q+T A++EIHV+YAG+L+ Sbjct: 301 LHILRNEPFKNAVNFPLVAASVMNKLQPYFSLGEKLGSVAAQITAHAVKEIHVDYAGELA 360 Query: 361 DVDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTLK 420 +VDTQ LTRYIVKGV RH G +VNIVNSMHLAK+RD++VVV++ KTK FTNL+TVTLK Sbjct: 361 EVDTQALTRYIVKGVLERHLGSEVNIVNSMHLAKSRDLHVVVSQTPKTKDFTNLVTVTLK 420 Query: 421 AEPDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQND 480 + ER+VAGTLL GYGERIV+++KFPVDI+PEGH I+ISHNDKPGIIG VGTLLG+ND Sbjct: 421 TQNGLERIVAGTLLLGYGERIVRLDKFPVDISPEGHLILISHNDKPGIIGRVGTLLGEND 480 Query: 481 VNIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVL 529 VNIASMQVGRK++GG AIMLLTVDK VP+ VL+KL GL E+NTA++I L Sbjct: 481 VNIASMQVGRKVIGGEAIMLLTVDKDVPKDVLIKLTGLSELNTAQQIAL 529 >B1DJ88.1 B1DJ88_9BACL D-3-phosphoglycerate dehydrogenase EC=1.1.1.95 Length = 530 Score = 840 bits (2169), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/531 (75%), Positives = 467/531 (87%), Gaps = 2/531 (0%) Query: 1 MFKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKII 60 MFKVLVSDPISDLGIQQL+DA DV VDKK GLSEDEL +IIGEYD LLVRSQT VT +I+ Sbjct: 1 MFKVLVSDPISDLGIQQLVDADDVAVDKKPGLSEDELVSIIGEYDALLVRSQTRVTPRIM 60 Query: 61 AAGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQA 120 AG +LKV+GRAGVGVDNI LEAATQ G++VINAPDGNTITTCEH FAMMMA+ARHIPQA Sbjct: 61 EAGKQLKVVGRAGVGVDNIDLEAATQRGIIVINAPDGNTITTCEHTFAMMMAVARHIPQA 120 Query: 121 YAKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAE 180 Y KT+ GVWDRK+FLGVELR KTLGVLGMGRIGSEVAKRAKAFGM I+ YDPF+T +RAE Sbjct: 121 YLKTVGGVWDRKSFLGVELRNKTLGVLGMGRIGSEVAKRAKAFGMEIMGYDPFMTEERAE 180 Query: 181 KLEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMA 240 KL +KLA+VD+IVR ADF+TVHTPLTPETRHMI + QFEVMK GMRI+NCARGG+IDE+A Sbjct: 181 KLGIKLATVDEIVRNADFMTVHTPLTPETRHMIGKKQFEVMKPGMRIINCARGGIIDELA 240 Query: 241 LVEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQV 300 LVEAID+GIVAGAAFDVFE EPP DHPFL+HPKIIVTPHLGASTVEAQENVAIDVSEQV Sbjct: 241 LVEAIDAGIVAGAAFDVFEVEPPTEDHPFLTHPKIIVTPHLGASTVEAQENVAIDVSEQV 300 Query: 301 LHILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLS 360 LHILRNEPF NAVN+PP+ ++ +KLQPY++LGEKLGS +Q+T GA++EI V Y+G+L+ Sbjct: 301 LHILRNEPFSNAVNMPPIPANLQSKLQPYYSLGEKLGSLVAQITEGAVQEITVRYSGELA 360 Query: 361 DVDTQPLTRYIVKGVFTRHFGGD-VNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTL 419 +VDTQPLTRYIVKGV + H G D VN+VNSMHLAK RDVN+V K+ +K FTNL+TV+L Sbjct: 361 EVDTQPLTRYIVKGVLSHHLGSDQVNVVNSMHLAKVRDVNIVQQKSQASKDFTNLVTVSL 420 Query: 420 KAEPDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQN 479 + + EER+VAGTLL GYGER+VQ++KFPVD+ PEG+ I+ISHNDKPGIIG VGTLLG N Sbjct: 421 RTK-QEERIVAGTLLTGYGERVVQIDKFPVDVTPEGNLILISHNDKPGIIGRVGTLLGNN 479 Query: 480 DVNIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVLL 530 DVNIA+MQVGR++VGG+AIM+LTVDK + +L +L L ++NTA+EI L Sbjct: 480 DVNIATMQVGRQLVGGSAIMVLTVDKNAGKDILEQLTSLSDLNTAKEITLF 530 >C0ZC90.1 C0ZC90_BREBN D-3-phosphoglycerate dehydrogenase EC=1.1.1.95 Length = 527 Score = 670 bits (1729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/529 (60%), Positives = 418/529 (79%), Gaps = 2/529 (0%) Query: 1 MFKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKII 60 M+KVL++DP+S+ GIQQL+DASDV V ++T LS EL +IG+YD LLVRSQT VT +++ Sbjct: 1 MYKVLITDPLSEFGIQQLLDASDVEVVRQTNLSPAELIDVIGDYDALLVRSQTQVTAEVL 60 Query: 61 AAGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQA 120 +AG +LK +GRAGVGVDNI + AATQ G+ VINAPDGNTI+T EH+FAM+MA+AR+IPQA Sbjct: 61 SAGKRLKAVGRAGVGVDNIDINAATQAGIPVINAPDGNTISTAEHSFAMLMAVARNIPQA 120 Query: 121 YAKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAE 180 + K + G WDRK+F GVEL K LGV+GMGRIGSEVAKRAKAFGM ++ +DPF+T +RA+ Sbjct: 121 HKKLVDGTWDRKSFQGVELNNKVLGVIGMGRIGSEVAKRAKAFGMTVMGFDPFMTEERAQ 180 Query: 181 KLEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMA 240 K+ V A+VD+I R ADFITVHTPLT ET H+IS +F MK G+R++NCARGG+IDE A Sbjct: 181 KMGVTNATVDEICRKADFITVHTPLTKETHHIISTREFAKMKDGVRLINCARGGIIDEKA 240 Query: 241 LVEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQV 300 L EAI +G VAGAA DVFE+EPP D+P + P+++ TPHLGAST+EAQENVA+DVSE++ Sbjct: 241 LYEAITTGKVAGAALDVFEEEPP-VDNPLVGLPQVVTTPHLGASTIEAQENVAVDVSEEI 299 Query: 301 LHILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLS 360 L +LRNEPF NAVN+P + VM K+QPYFTLGEKLG F +Q+T G+I EI ++Y+G+L+ Sbjct: 300 LKVLRNEPFKNAVNLPSIPAHVMEKVQPYFTLGEKLGHFLAQVTVGSISEISIKYSGELT 359 Query: 361 DVDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTLK 420 DVDT PLTR ++KGV + G +VN VN+ LAK RD+ V K ++ KGFTNL+TV+LK Sbjct: 360 DVDTSPLTRTVLKGVLSFRLGEEVNYVNAPILAKVRDITVTEQKTAQNKGFTNLLTVSLK 419 Query: 421 AEPDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQND 480 E R VAGT L GYG RIV+++ F +D+APEG+ + I HND+PG+IG VG++LG+N Sbjct: 420 TT-QETRTVAGTRLNGYGARIVKIDDFAIDVAPEGYLLYIHHNDRPGVIGRVGSILGENS 478 Query: 481 VNIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVL 529 VNIA+MQVGR+ +GG AIM+L+VDK + ++L + L E+ + +I L Sbjct: 479 VNIATMQVGRRDIGGDAIMMLSVDKPLTPELLDTMGELAEVKSVTQIEL 527 >Q8EN61.1 Q8EN61_OCEIH Phosphoglycerate dehydrogenase EC=1.1.1.95 Length = 528 Score = 558 bits (1439), Expect = e-157, Method: Compositional matrix adjust. Identities = 273/528 (51%), Positives = 379/528 (71%), Gaps = 2/528 (0%) Query: 2 FKVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKIIA 61 F VL+SDP+S+ G++ L +A ++ V G +E EL+ I +D +LVRSQT VT +I Sbjct: 3 FHVLISDPLSEEGLKPLQEAENIEVVINPGWNEQELSDQIDSFDAILVRSQTQVTRALIE 62 Query: 62 AGTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQAY 121 + LK+IGRAGVGVDNI LEAAT++GV+V+NAP+GNT + EH AM+MAL+R+IPQAY Sbjct: 63 KASNLKIIGRAGVGVDNIDLEAATENGVIVVNAPNGNTNSAAEHTMAMIMALSRNIPQAY 122 Query: 122 AKTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAEK 181 WDRK F+GVEL+ KTLG++G+GRIG+EVA RAK MN++AYDPF T ++AE+ Sbjct: 123 HALKQKQWDRKRFVGVELKQKTLGIVGLGRIGAEVAARAKGQRMNVIAYDPFFTEEKAEQ 182 Query: 182 LEVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMAL 241 + V+ +++D++R DFITVHTPL ET+H+I++ F++MK G++IVNCARGG+IDE AL Sbjct: 183 MGVQYGTLEDVLRAGDFITVHTPLLKETKHLINKDAFDLMKDGVQIVNCARGGIIDEDAL 242 Query: 242 VEAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQVL 301 +AI SG VAGAA DVFE+EP +H L+ P+++ TPHLGASTVEAQE VAIDVS VL Sbjct: 243 YDAIQSGKVAGAALDVFEQEPF-TEHKLLTLPEVVATPHLGASTVEAQEVVAIDVSHDVL 301 Query: 302 HILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLSD 361 + E N VN+P V +M K++PYF L EKLG+F + LT + E+ + YAGDL+D Sbjct: 302 RFSQGEAVRNPVNMPSVPSEIMYKIEPYFHLAEKLGTFITDLTKEVVSEVKITYAGDLAD 361 Query: 362 VDTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTLKA 421 ++ PLTR VKG+ R+ G +N VN++ LA + + V +K S KGFTNLIT+ + Sbjct: 362 IEIAPLTRNAVKGLLKRYLGSRINDVNALFLADRKGIEVSESKTSSKKGFTNLITIEVSG 421 Query: 422 EPDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQNDV 481 + R VAGTLL G G R+V+V+++ VDI PEGH +VI H D+PG+IG +G+ + ++D+ Sbjct: 422 KFG-TRKVAGTLLNGLGPRLVRVDEYRVDITPEGHMVVIQHRDQPGVIGRMGSTIAKHDI 480 Query: 482 NIASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVL 529 NIA+MQV R +GG AIM+LT+D+ + + L ++ L EI + I L Sbjct: 481 NIATMQVDRSDIGGDAIMILTIDRHLADEALNEIESLDEIKSVTAIDL 528 >B7DRL0.1 B7DRL0_9BACL D-3-phosphoglycerate dehydrogenase EC=1.1.1.95 Length = 529 Score = 556 bits (1433), Expect = e-156, Method: Compositional matrix adjust. Identities = 279/527 (52%), Positives = 370/527 (70%), Gaps = 1/527 (0%) Query: 3 KVLVSDPISDLGIQQLMDASDVTVDKKTGLSEDELTAIIGEYDGLLVRSQTTVTEKIIAA 62 K+LV+D IS GI L V +T L+ DEL I + D L+VRSQT VT +I + Sbjct: 4 KILVTDDISQAGIDILSGLEGAEVVVRTNLASDELKEAIADADALVVRSQTRVTRDVIES 63 Query: 63 GTKLKVIGRAGVGVDNIKLEAATQHGVVVINAPDGNTITTCEHAFAMMMALARHIPQAYA 122 KLKVIGRAGVGVDNI LEAAT+ G++VINAPDGNTI EH FAMM++LARHIP A+ Sbjct: 64 AKKLKVIGRAGVGVDNIDLEAATRRGILVINAPDGNTIAAAEHTFAMMISLARHIPAAHR 123 Query: 123 KTISGVWDRKTFLGVELRGKTLGVLGMGRIGSEVAKRAKAFGMNILAYDPFLTADRAEKL 182 + G W+RK ++GVELRGKTL VLGMGRIG+EVAKRAKAFGM++L YDPFLT +RA+ L Sbjct: 124 DLLQGHWNRKKWIGVELRGKTLAVLGMGRIGTEVAKRAKAFGMHVLGYDPFLTEERAQSL 183 Query: 183 EVKLASVDDIVRGADFITVHTPLTPETRHMISRPQFEVMKKGMRIVNCARGGVIDEMALV 242 V +D +R ADFITVHTPLT ET HMI + +MK+G+RI+NCARGG+IDE+AL Sbjct: 184 GVTRTDLDTAIRQADFITVHTPLTKETHHMIDVGRIALMKEGVRIINCARGGIIDEVALA 243 Query: 243 EAIDSGIVAGAAFDVFEKEPPQADHPFLSHPKIIVTPHLGASTVEAQENVAIDVSEQVLH 302 EA+++G VAGAA DVFE+EP DHP P +++TPHLGASTVEAQENVAI V+E+++ Sbjct: 244 EALEAGRVAGAAIDVFEQEPLPMDHPLRRCPNVVLTPHLGASTVEAQENVAIQVAEEIVQ 303 Query: 303 ILRNEPFINAVNIPPVAPSVMNKLQPYFTLGEKLGSFASQLTGGAIREIHVEYAGDLSDV 362 +LR++ F +AVN+P ++ +L PY L E+LG FA+QL GA + V YAGD +D Sbjct: 304 VLRDDTFEHAVNLPSLSQRQKERLAPYLALAEQLGLFAAQLAQGAPSSMTVRYAGDAADP 363 Query: 363 DTQPLTRYIVKGVFTRHFGGDVNIVNSMHLAKTRDVNVVVTKASKTKGFTNLITVTLKAE 422 D LTR ++KG F+ + G+VN VN++ A+ + V + S+ + +TN + +++ + Sbjct: 364 DGGYLTRTVLKGFFSFQYNGEVNYVNALRYAEDAGLRVQEVRESRGRVYTNEVEISVATD 423 Query: 423 PDEERLVAGTLLQGYGERIVQVNKFPVDIAPEGHQIVISHNDKPGIIGLVGTLLGQNDVN 482 R V GT+L YG RIV+++ +P+D +G I H D+PG+IG +GTLLG D+N Sbjct: 424 NGTHR-VTGTVLGEYGPRIVELDGYPIDTPIQGILIYTRHEDRPGMIGRIGTLLGDRDIN 482 Query: 483 IASMQVGRKIVGGAAIMLLTVDKAVPQQVLVKLAGLPEINTAEEIVL 529 IA MQVGR+ GG A+MLL+VDK+VPQ V+ ++A P I I L Sbjct: 483 IAGMQVGRRETGGEAVMLLSVDKSVPQDVIDEIAKHPGIRLVRSIEL 529 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,017,002,585 Number of extensions: 126947644 Number of successful extensions: 385608 Number of sequences better than 1.0e-05: 7260 Number of HSP's gapped: 371493 Number of HSP's successfully gapped: 7287 Length of query: 530 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 389 Effective length of database: 1,732,333,088 Effective search space: 673877571232 Effective search space used: 673877571232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)