BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0002.p (278 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL76.1 B3KL76_9BACI Metallophosphoesterase 203 2e-50 B7GHL8.1 B7GHL8_ANOFW Calcineurin-like phosphohydrolase 134 6e-30 Q2B642.1 Q2B642_9BACI Phosphoesterase-related protein 127 1e-27 C3BI64.1 C3BI64_9BACI Phosphoesterase 122 5e-26 C3B1F5.1 C3B1F5_BACMY Phosphoesterase 122 5e-26 >B3KL76.1 B3KL76_9BACI Metallophosphoesterase Length = 272 Score = 203 bits (516), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 110/241 (45%), Positives = 149/241 (61%), Gaps = 4/241 (1%) Query: 36 EEIFLEALPKEFDGFRILFISDIHRRRLPFRRLSPMKGEIDAVFLGGDLTEKGASMNRLY 95 EE+ E +P+ FDGFR+LFI+DIHRR+L +L +D V LGGD+TEKG RL Sbjct: 33 EEVVSERIPRSFDGFRMLFITDIHRRQLSEEKLLSNISSVDCVLLGGDITEKGVPFERLR 92 Query: 96 DNMTXXXXXXXXXXVHGNHDYRANITLVDNIIRGSGVKLLQDESITIQKNGSSLLLTGVD 155 NM+ V GNHD A V ++R SGV LL D+++T++++G L+L+GV Sbjct: 93 HNMSVLARLAPVYAVLGNHDLYAGKDAVRKVLRESGVILLNDKTVTLKRDGECLVLSGVR 152 Query: 156 FPRTGGKRAYRPLPPLSSAEAPFCRIILVHDPLWLTQQSEVPADLVLAGHTHGGQVVLPF 215 P K Y ++AE + IILVHDP+WL + E DL+LAGHTHGGQ+VLP Sbjct: 153 QP-ASRKHPYTKFRG-NAAEDQY-HIILVHDPIWLKGKEETHGDLLLAGHTHGGQIVLPI 209 Query: 216 VGHRHVDPFYRQYNAGHYVIPRN-DGSGKKAGLLISRGFGTAHFPLRWGSRAEMHVLTLR 274 G ++ FY+++ +G Y +PR S + LLISRGFGT+H PLR AE HV+TLR Sbjct: 210 AGAVRLERFYKKFKSGWYTLPRKASESVHSSRLLISRGFGTSHIPLRLKCPAEYHVITLR 269 Query: 275 R 275 + Sbjct: 270 K 270 >B7GHL8.1 B7GHL8_ANOFW Calcineurin-like phosphohydrolase Length = 262 Score = 134 bits (338), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 92/251 (36%), Positives = 131/251 (52%), Gaps = 24/251 (9%) Query: 28 AFRNHIIAEEIFLEALPKEFDGFRILFISDIHRRRLPFRRLSPMKGEIDAVFLGGDLTEK 87 A RN +I + P+ F FRI FISDIHRRR+ + +KG++D V +GGDLTEK Sbjct: 28 AHRNRVIFHSLPFPTFPESFSRFRIFFISDIHRRRVSKTLIGSLKGKVDLVIIGGDLTEK 87 Query: 88 GASMNRLYDNMTXXXXXXXXXXVHGNHDYRANITLVDNIIRGSGVKLLQDESITIQ-KNG 146 G +R+ N+ V GN+DY + +D ++ +GVK+L + ++T + + G Sbjct: 88 GVPFSRVKRNIELLKQLGPVYFVWGNNDYEVDYHELDALLLENGVKILDNTAVTFESEQG 147 Query: 147 SSLLLTGVDFPRTGGKRAYRPLPPLSSAEAPFCRIILVHDPLWLTQ---QSEVPADLVLA 203 + L GVD KR R LS AE RI++ HDP + + Q E+ LVL+ Sbjct: 148 DRIALLGVD---DMNKRRDRLELALSDAEHTTFRILVSHDPRIIEKIKPQHEIA--LVLS 202 Query: 204 GHTHGGQVVLPFVGHRHVDPFYRQYNAGHYVIPRNDGSGKKAGLLISRGFGTAHFPLRWG 263 GHTHGGQ+ L G Y G RN LL+S G+GT P R+G Sbjct: 203 GHTHGGQIRLGPFG---------LYEKG---CLRNING---TMLLVSNGYGTTTLPFRFG 247 Query: 264 SRAEMHVLTLR 274 +RAE H++ L+ Sbjct: 248 ARAETHLIELK 258 >Q2B642.1 Q2B642_9BACI Phosphoesterase-related protein Length = 260 Score = 127 bits (319), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 87/250 (34%), Positives = 130/250 (52%), Gaps = 20/250 (8%) Query: 28 AFRNHIIAEEIFLEALPKEFDGFRILFISDIHRRRLPFRRLSPMKGEIDAVFLGGDLTEK 87 AF++ +I E+ E P F I FISDIHRR++ + L +KG+ D V +GGDL EK Sbjct: 27 AFQDRVIHNELEFEDFPAGFGSVTIFFISDIHRRKVSDKLLEQVKGKADLVIIGGDLAEK 86 Query: 88 GASMNRLYDNMTXXXXXXXXXXVHGNHDYRANITLVDNIIRGSGVKLLQDESITIQ-KNG 146 G + + +N+ V GN+DY + +D ++ SGVK+L + +++ + +NG Sbjct: 87 GVPFSLVKENIEKLKTVGPAYFVWGNNDYELDHRELDALLLESGVKILDNTAVSFEAENG 146 Query: 147 SSLLLTGVDFPRTGGKRAYRPLPPLSSAEAPFCRIILVHDPLWLTQ-QSEVPADLVLAGH 205 +++L GVD GK R L A RI+ H+P + + + LVL+GH Sbjct: 147 DTMILMGVD---DVGKGRDRLDLALQDAGPSGFRILACHNPSIIGKIEKRHEIRLVLSGH 203 Query: 206 THGGQVVLPFVGHRHVDPFYRQYNAGHYVIPRNDGSGKKAGLLISRGFGTAHFPLRWGSR 265 THGGQ+ H+ Y Y G + +A LLIS G+GT PLR G+R Sbjct: 204 THGGQI--------HI-LGYSPYEKGKV------KTMDRATLLISNGYGTTALPLRLGAR 248 Query: 266 AEMHVLTLRR 275 AE H++ L R Sbjct: 249 AETHLVRLGR 258 >C3BI64.1 C3BI64_9BACI Phosphoesterase Length = 257 Score = 122 bits (305), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 87/273 (31%), Positives = 132/273 (48%), Gaps = 22/273 (8%) Query: 4 VWLLGIAGICVXXXXXXXXXXXAAAFRNHIIAEEIFLEALPKEFDGFRILFISDIHRRRL 63 +W+LG IC+ A RN ++ + P+ F RI FISDIHRR + Sbjct: 2 LWILGGTFICIGFICLFMMYR--EALRNTLLEHTLSFVNFPESFGKVRIFFISDIHRRIV 59 Query: 64 PFRRLSPMKGEIDAVFLGGDLTEKGASMNRLYDNMTXXXXXXXXXXVHGNHDYRANITLV 123 + +KG+ D V +GGDL EKG S++++ N+ V GN+DY + + Sbjct: 60 SPSLIENVKGKADFVIIGGDLAEKGVSLSQIAANVQTLSKIGPIYFVWGNNDYEIDYHEL 119 Query: 124 DNIIRGSGVKLLQDESITIQKN-GSSLLLTGVDFPRTGGKRAYRPLPPLSSAEAPFCRII 182 D ++ GVK+L + + + + G L L GVD G +R L E F RI+ Sbjct: 120 DALLLEHGVKILDNTRVLFESDSGDKLCLLGVD--DVGLERDRLDLALADCQENGF-RIL 176 Query: 183 LVHDPLWLTQQS-EVPADLVLAGHTHGGQVVLPFVGHRHVDPFYRQYNAGHYVIPRNDGS 241 + H+P+ + + S + LVL+GHTHGGQ+ L F R G Y P Sbjct: 177 ISHNPVIVNKMSGQEQISLVLSGHTHGGQIRLF--------HFKRYLKGGVYKYP----- 223 Query: 242 GKKAGLLISRGFGTAHFPLRWGSRAEMHVLTLR 274 L +S G+GT PLR+ + A+ H++TL+ Sbjct: 224 --NTTLFVSNGYGTTLIPLRFRAPAQTHLITLQ 254 >C3B1F5.1 C3B1F5_BACMY Phosphoesterase Length = 257 Score = 122 bits (305), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 87/273 (31%), Positives = 132/273 (48%), Gaps = 22/273 (8%) Query: 4 VWLLGIAGICVXXXXXXXXXXXAAAFRNHIIAEEIFLEALPKEFDGFRILFISDIHRRRL 63 +W+LG IC+ A RN ++ + P+ F RI FISDIHRR + Sbjct: 2 LWILGGTFICIGFICLFMMYR--EALRNTLLEHTLSFVNFPESFGKVRIFFISDIHRRVV 59 Query: 64 PFRRLSPMKGEIDAVFLGGDLTEKGASMNRLYDNMTXXXXXXXXXXVHGNHDYRANITLV 123 + +KG+ D V +GGDL EKG S++++ N+ V GN+DY + + Sbjct: 60 SPSLIENVKGKADFVIIGGDLAEKGVSLSQIAANVQTLSKIGPIYFVWGNNDYEIDYHEL 119 Query: 124 DNIIRGSGVKLLQDESITIQKN-GSSLLLTGVDFPRTGGKRAYRPLPPLSSAEAPFCRII 182 D ++ GVK+L + + + + G L L GVD G +R L E F RI+ Sbjct: 120 DALLLEHGVKILDNTRVLFESDSGDKLCLLGVD--DVGLERDRLDLALADCQENGF-RIL 176 Query: 183 LVHDPLWLTQQS-EVPADLVLAGHTHGGQVVLPFVGHRHVDPFYRQYNAGHYVIPRNDGS 241 + H+P+ + + S + LVL+GHTHGGQ+ L F R G Y P Sbjct: 177 ISHNPVIVNKMSGQEQISLVLSGHTHGGQIRLF--------HFKRYLKGGVYKYP----- 223 Query: 242 GKKAGLLISRGFGTAHFPLRWGSRAEMHVLTLR 274 L +S G+GT PLR+ + A+ H++TL+ Sbjct: 224 --NTTLFVSNGYGTTLIPLRFRAPAQTHLITLQ 254 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.142 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,622,915,945 Number of extensions: 65851018 Number of successful extensions: 161451 Number of sequences better than 1.0e-05: 700 Number of HSP's gapped: 160637 Number of HSP's successfully gapped: 700 Length of query: 278 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 144 Effective length of database: 1,798,280,193 Effective search space: 258952347792 Effective search space used: 258952347792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)