BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0012.p (274 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D5Y0.1 C5D5Y0_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 359 1e-97 A4IKN3.1 A4IKN3_GEOTN Oxidoreductase, AldO/KetO reductase family 351 5e-95 B4BRY0.1 B4BRY0_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 351 5e-95 A7Z8R2.1 A7Z8R2_BACA2 YvgN 342 3e-92 O32210.1 GR_BACSU Glyoxal reductase EC=1.1.1.- EC=1.1.1.283 341 5e-92 >C5D5Y0.1 C5D5Y0_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 Length = 275 Score = 359 bits (922), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 169/275 (61%), Positives = 207/275 (75%), Gaps = 1/275 (0%) Query: 1 MKHIADVTILNNGIAMPRFGLGTYKAE-GEEVARAVATALELGYRSIDTAAVYGNEEEVG 59 MKH+ D L+NG+ MP GLG YK + GEEV AV TALE+GYR IDTAA Y NEE VG Sbjct: 1 MKHLQDRVTLHNGVQMPWLGLGVYKVKNGEEVINAVRTALEIGYRHIDTAAFYENEEGVG 60 Query: 60 RSIAASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKA 119 +++ SG+PR+ +F+TTKVWNSDQGY+ TL+AFETS +KLGLD +DLYL+HWP KYK Sbjct: 61 QAVRESGIPREEIFITTKVWNSDQGYETTLKAFETSLQKLGLDYVDLYLVHWPVKGKYKE 120 Query: 120 TWRALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCAS 179 T++ALE+LY +GRVRAIGVSNFQIHHLE+L D E P VNQVE HPR QKELH +C Sbjct: 121 TYKALEKLYKDGRVRAIGVSNFQIHHLEDLMADCEIKPMVNQVEYHPRLTQKELHAFCKQ 180 Query: 180 RQIQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKE 239 IQ+EAW+PLM+G + L I K+GKT +QV+LRW LQN +V IPKSVT RIKE Sbjct: 181 HGIQLEAWSPLMRGEILQEPTLVEIGRKYGKTPAQVVLRWDLQNEVVTIPKSVTPQRIKE 240 Query: 240 NSEIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 N+ +FDFEL+AEE++ I L+ +RIG DPD F Sbjct: 241 NANVFDFELTAEEMAKIDALNLNKRIGPDPDNFDF 275 >A4IKN3.1 A4IKN3_GEOTN Oxidoreductase, AldO/KetO reductase family Length = 275 Score = 351 bits (900), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 165/275 (60%), Positives = 205/275 (74%), Gaps = 1/275 (0%) Query: 1 MKHIADVTILNNGIAMPRFGLGTYKA-EGEEVARAVATALELGYRSIDTAAVYGNEEEVG 59 M + D T+L+NG+ MP GLG YK EGEEV AV TALE+GYR IDTAA Y NEE VG Sbjct: 1 MNSLQDCTVLHNGVKMPWVGLGVYKVKEGEEVKGAVRTALEVGYRHIDTAAFYENEEGVG 60 Query: 60 RSIAASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKA 119 ++I SG+PR+ +FVTTKVWN+DQGY+ TL+AF TS KKLG D +DLYL+HWP KYK Sbjct: 61 QAIRESGIPREQVFVTTKVWNTDQGYETTLKAFNTSLKKLGFDYVDLYLVHWPVKGKYKE 120 Query: 120 TWRALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCAS 179 T++ALE+LY +G VRAIGVSNFQIHHL+++ D E P VNQVE HPR QKELH +C Sbjct: 121 TYKALEKLYKDGYVRAIGVSNFQIHHLQDVLADCEIKPMVNQVEYHPRLTQKELHAFCRE 180 Query: 180 RQIQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKE 239 IQ+EAW+PLM+G + + + I K+GKT +QV+LRW LQNG+V IPKSVT +RIKE Sbjct: 181 NGIQLEAWSPLMRGEILNEPTIVEIGKKYGKTPAQVVLRWDLQNGVVTIPKSVTPARIKE 240 Query: 240 NSEIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 N+ IFDF L+ EE+ I L+ +RIG+DPD F Sbjct: 241 NATIFDFSLTDEEMRQIDALNLNKRIGADPDNFNF 275 >B4BRY0.1 B4BRY0_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 Length = 275 Score = 351 bits (900), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 165/275 (60%), Positives = 205/275 (74%), Gaps = 1/275 (0%) Query: 1 MKHIADVTILNNGIAMPRFGLGTYKA-EGEEVARAVATALELGYRSIDTAAVYGNEEEVG 59 M + D T+L+NG+ MP GLG YK EGEEV AV TALE+GYR IDTAA Y NEE VG Sbjct: 1 MNSLQDCTVLHNGVKMPWVGLGVYKVKEGEEVKGAVRTALEVGYRHIDTAAFYENEEGVG 60 Query: 60 RSIAASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKA 119 ++I SG+PR+ +FVTTKVWN+DQGY+ TL+AF TS KKLG D +DLYL+HWP KYK Sbjct: 61 QAIRESGIPREQVFVTTKVWNTDQGYETTLKAFNTSLKKLGFDYVDLYLVHWPVKGKYKE 120 Query: 120 TWRALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCAS 179 T++ALE+LY +G VRAIGVSNFQIHHL+++ D E P VNQVE HPR QKELH +C Sbjct: 121 TYKALEKLYKDGYVRAIGVSNFQIHHLQDVLADCEIKPMVNQVEYHPRLTQKELHAFCRE 180 Query: 180 RQIQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKE 239 IQ+EAW+PLM+G + + + I K+GKT +QV+LRW LQNG+V IPKSVT +RIKE Sbjct: 181 NGIQLEAWSPLMRGEILNEPTIVEIGKKYGKTPAQVVLRWDLQNGVVTIPKSVTPARIKE 240 Query: 240 NSEIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 N+ IFDF L+ EE+ I L+ +RIG+DPD F Sbjct: 241 NATIFDFSLTDEEMRQIDALNLNKRIGADPDNFNF 275 >A7Z8R2.1 A7Z8R2_BACA2 YvgN Length = 276 Score = 342 bits (876), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 169/274 (61%), Positives = 211/274 (77%), Gaps = 1/274 (0%) Query: 2 KHIADVTILNNGIAMPRFGLGTYKAE-GEEVARAVATALELGYRSIDTAAVYGNEEEVGR 60 K + D L+NG+ MP FGLG +K E G A +V A+ GYRSIDTAA+Y NEE VG Sbjct: 3 KSLKDTVKLHNGVEMPWFGLGVFKVENGSVAADSVEAAIRNGYRSIDTAAIYKNEEGVGE 62 Query: 61 SIAASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKAT 120 I A+GV R+ LF+T+KVWN DQGY+ TL AFE S ++LGLD +DLYLIHWPG DKYK T Sbjct: 63 GIRAAGVAREELFITSKVWNEDQGYETTLDAFEKSLERLGLDYLDLYLIHWPGKDKYKDT 122 Query: 121 WRALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCASR 180 WRALE+LY +G++RAIGVSNFQ+HHLEEL KD+E P VNQ+E HPR QKE+ D+C ++ Sbjct: 123 WRALEKLYKDGKIRAIGVSNFQVHHLEELLKDAEIKPMVNQIEFHPRLTQKEVRDFCKAQ 182 Query: 181 QIQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKEN 240 IQ+EAW+PLM+G+L DNE+L IA KH K+V+QVILRW LQN +V IPKS+ RI EN Sbjct: 183 GIQVEAWSPLMQGQLLDNEVLSQIAEKHNKSVAQVILRWDLQNEVVTIPKSIKEHRIIEN 242 Query: 241 SEIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 ++IFDFELS EE+ IS L+ ER+G +PD+LLF Sbjct: 243 ADIFDFELSQEEMEKISALNKDERVGPNPDELLF 276 >O32210.1 GR_BACSU Glyoxal reductase EC=1.1.1.- EC=1.1.1.283 Length = 276 Score = 341 bits (874), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 168/272 (61%), Positives = 208/272 (76%), Gaps = 1/272 (0%) Query: 4 IADVTILNNGIAMPRFGLGTYKAE-GEEVARAVATALELGYRSIDTAAVYGNEEEVGRSI 62 + D L+NG+ MP FGLG +K E G E +V A++ GYRSIDTAA+Y NEE VG I Sbjct: 5 LKDTVKLHNGVEMPWFGLGVFKVENGNEATESVKAAIKNGYRSIDTAAIYKNEEGVGIGI 64 Query: 63 AASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKATWR 122 SGV R+ LF+T+KVWN DQGY+ TL AFE S ++L LD +DLYLIHWPG DKYK TWR Sbjct: 65 KESGVAREELFITSKVWNEDQGYETTLAAFEKSLERLQLDYLDLYLIHWPGKDKYKDTWR 124 Query: 123 ALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCASRQI 182 ALE+LY +G++RAIGVSNFQ+HHLEEL KD+E P VNQVE HPR QKEL DYC + I Sbjct: 125 ALEKLYKDGKIRAIGVSNFQVHHLEELLKDAEIKPMVNQVEFHPRLTQKELRDYCKGQGI 184 Query: 183 QIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKENSE 242 Q+EAW+PLM+G+L DNE+L IA KH K+V+QVILRW LQ+G+V IPKS+ RI EN++ Sbjct: 185 QLEAWSPLMQGQLLDNEVLTQIAEKHNKSVAQVILRWDLQHGVVTIPKSIKEHRIIENAD 244 Query: 243 IFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 IFDFELS E++ I L+ ER+G +PD+LLF Sbjct: 245 IFDFELSQEDMDKIDALNKDERVGPNPDELLF 276 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,594,066,145 Number of extensions: 63895242 Number of successful extensions: 193611 Number of sequences better than 1.0e-05: 6193 Number of HSP's gapped: 183193 Number of HSP's successfully gapped: 6275 Length of query: 274 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 140 Effective length of database: 1,798,280,193 Effective search space: 251759227020 Effective search space used: 251759227020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)