BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0004.p (412 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLA9.1 B3KLA9_9BACI Major facilitator superfamily MFS_1 558 e-157 Q9K9Q3.1 Q9K9Q3_BACHD BH2592 protein 334 1e-89 C0Z656.1 C0Z656_BREBN Probable multidrug resistance protein 291 9e-77 A0AM75.1 A0AM75_LISW6 Complete genome 264 2e-68 B8GI60.1 B8GI60_METPE Major facilitator superfamily MFS_1 262 4e-68 >B3KLA9.1 B3KLA9_9BACI Major facilitator superfamily MFS_1 Length = 434 Score = 558 bits (1437), Expect = e-157, Method: Compositional matrix adjust. Identities = 286/412 (69%), Positives = 318/412 (77%), Gaps = 4/412 (0%) Query: 2 KGSESTLKNWETWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGD-AIGLWAGL 60 K +STL NW K+NLIVLWFGQFLVN+GMTMITPFLTLYLA+DL V GD IG+WAGL Sbjct: 26 KEVKSTLTNW---KINLIVLWFGQFLVNSGMTMITPFLTLYLAQDLNVVGDREIGVWAGL 82 Query: 61 IFAANFMTSFLFQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNG 120 IFAANF+TSF+FQP+WGK+ADKYGRKIMLLRS FGMALVI MGFA PW NG Sbjct: 83 IFAANFLTSFIFQPIWGKVADKYGRKIMLLRSGFGMALVIACMGFATHPWHLLALRLLNG 142 Query: 121 TISGFNPASIALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIV 180 TISGFNPA+I+LVSG TPK RMGFAMG+MQSG VAGTILGPLIGG +A W+G+RPIFYI Sbjct: 143 TISGFNPAAISLVSGTTPKNRMGFAMGIMQSGQVAGTILGPLIGGLMASWVGFRPIFYIT 202 Query: 181 GXXXXXXXXXXXXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMI 240 G VKE F+R AA+ PQ SV+ G KEL+ +PQLPALF VTFLLQFAMI Sbjct: 203 GGLIFTASLLALFLVKENFNRERAAKAPQPSVIAGLKELSHIPQLPALFAVTFLLQFAMI 262 Query: 241 APMTLLPLYVEKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALI 300 +PM LLPLYV+KLHGSAVDIA WAG V AVTG+SNM+ASP+LGKLSDKVGAHRILTYALI Sbjct: 263 SPMALLPLYVQKLHGSAVDIAIWAGFVVAVTGLSNMIASPILGKLSDKVGAHRILTYALI 322 Query: 301 GAALFIIPQAFVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSML 360 G L +IPQAFV SVWQLIIVRF+MGVFMGGLLPSVNALIRSYTPDG ESR+FGFN+S L Sbjct: 323 GTGLTLIPQAFVQSVWQLIIVRFMMGVFMGGLLPSVNALIRSYTPDGMESRSFGFNTSSL 382 Query: 361 ALGNMLGAVXXXXXXXXXXXXXXXXXXXXXXXXNTVWVRLKLYKGAAPRLFR 412 ALGNMLGAV N +WVR KL+ +R Sbjct: 383 ALGNMLGAVIGGFLSGFIGIEGLFITSGCLLIINMLWVRRKLFSHKPSPKYR 434 >Q9K9Q3.1 Q9K9Q3_BACHD BH2592 protein Length = 409 Score = 334 bits (856), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 177/355 (49%), Positives = 242/355 (68%), Gaps = 5/355 (1%) Query: 14 WKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQG-DAIGLWAGLIFAANFMTSFLF 72 WK NL +L QFLV + MTMI PFL LYL ++LGV + LWAG+IFAANF+T+FLF Sbjct: 4 WKRNLYILVVCQFLVVSAMTMIIPFLPLYL-QELGVTDPSEVSLWAGIIFAANFLTAFLF 62 Query: 73 QPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASIAL 132 PLW +LAD+YGRK+M+LRS FGMAL+I L GFAQ P NG ISGF PASI L Sbjct: 63 SPLWSRLADRYGRKMMILRSGFGMALIIGLTGFAQGPLSLLCLRLLNGVISGFIPASIGL 122 Query: 133 VSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXXXX 192 V+ NTPK + G+A+G +Q+G+V+G I GPLIGG +A GYR IF++ G Sbjct: 123 VATNTPKEKAGYALGFLQAGAVSGGICGPLIGGIMAQAFGYRMIFFLTGLCIFIAALVVL 182 Query: 193 XXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYVEK 252 V E+F + + + FK++ + + +L+ V FL+QFA++ L+ L+VE+ Sbjct: 183 LFVNEQFTPNPKEE--KTNTWGEFKKITAIRPIFSLYVVVFLIQFAVMGVNPLISLFVEE 240 Query: 253 LHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQAFV 312 L S V++A +AG+ + TG +NML+S LGKLSDK GAH +L + L+G +F+IPQAFV Sbjct: 241 LV-SGVNVALFAGLAISATGFANMLSSMQLGKLSDKAGAHHVLFFCLLGTVIFMIPQAFV 299 Query: 313 TSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 +WQLI++RF +G+ +GGLLP+ NAL+R + P G ESR +GF++S L LGNM+G Sbjct: 300 QQLWQLIVLRFFVGLSLGGLLPAANALVRMHAPKGMESRTYGFSTSALYLGNMVG 354 Score = 60.5 bits (145), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 45/155 (29%), Positives = 72/155 (46%), Gaps = 3/155 (1%) Query: 26 FLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLFQPLWGKLADKYGR 85 FL+ + + P ++L++ + V G + L+AGL +A + L GKL+DK G Sbjct: 221 FLIQFAVMGVNPLISLFVEEL--VSGVNVALFAGLAISATGFANMLSSMQLGKLSDKAGA 278 Query: 86 KIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNG-TISGFNPASIALVSGNTPKARMGF 144 +L G + ++ F Q WQ G ++ G PA+ ALV + PK Sbjct: 279 HHVLFFCLLGTVIFMIPQAFVQQLWQLIVLRFFVGLSLGGLLPAANALVRMHAPKGMESR 338 Query: 145 AMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYI 179 G S G ++GP +GGA+ +G R +F I Sbjct: 339 TYGFSTSALYLGNMVGPTVGGAVVATVGIRGLFLI 373 >C0Z656.1 C0Z656_BREBN Probable multidrug resistance protein Length = 406 Score = 291 bits (745), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 166/358 (46%), Positives = 234/358 (65%), Gaps = 4/358 (1%) Query: 12 ETWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQG-DAIGLWAGLIFAANFMTSF 70 E W+ NL VL F V M+MI PFL LY+ +D G++ + WAG+IF ANF+T+ Sbjct: 2 EIWRRNLYVLCGSLFFVMVAMSMIMPFLPLYIQQDFGIEDPHQVTAWAGIIFGANFLTAG 61 Query: 71 LFQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASI 130 L P+WG LADK+GRKIM+LRS F M++ + + GFA + WQ NG + G PAS Sbjct: 62 LVSPIWGNLADKHGRKIMILRSGFFMSITMAMTGFAGSLWQLLALRLLNGLVGGIIPAST 121 Query: 131 ALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXX 190 ALV+ + PK R+G+A GL+QS AGTI+GPL GG LA+ IG+R IF I G Sbjct: 122 ALVASSVPKERIGYATGLLQSFITAGTIMGPLFGGVLAEKIGFRMIFVITGCVLLLATLV 181 Query: 191 XXXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYV 250 VKE F E + Q S+ E F+ + +LPALF VT ++QFA+ + + +LP+Y+ Sbjct: 182 ITFTVKENFVPPEKKE--QSSLREDFQMIFSSKELPALFFVTVMIQFALFSIIPVLPIYI 239 Query: 251 EKLHGS-AVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQ 309 +L GS +A WAG+V A GV+N+ ASP LG+L D+ G+ ++L ++L+ AA+ IPQ Sbjct: 240 SQLLGSEGAKVALWAGIVQACMGVANVFASPQLGRLGDRFGSQKVLLFSLLAAAIIFIPQ 299 Query: 310 AFVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 V +VWQL+ +RFL+G+ +GGLLPSVNAL+R TP SR +G+N+S +++G+MLG Sbjct: 300 GLVHTVWQLVALRFLLGLSLGGLLPSVNALLRRATPVHMVSRVYGYNNSFVSIGSMLG 357 Score = 69.7 bits (169), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 51/184 (27%), Positives = 91/184 (49%), Gaps = 8/184 (4%) Query: 2 KGSESTLKNWETWKV-----NLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGL 56 K +S+L+ E +++ L L+F ++ + I P L +Y+++ LG +G + L Sbjct: 195 KKEQSSLR--EDFQMIFSSKELPALFFVTVMIQFALFSIIPVLPIYISQLLGSEGAKVAL 252 Query: 57 WAGLIFAANFMTSFLFQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXX 116 WAG++ A + + P G+L D++G + +LL S A++ + G T WQ Sbjct: 253 WAGIVQACMGVANVFASPQLGRLGDRFGSQKVLLFSLLAAAIIFIPQGLVHTVWQLVALR 312 Query: 117 -XXNGTISGFNPASIALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRP 175 ++ G P+ AL+ TP + G S G++LGP+IGG LA ++ Sbjct: 313 FLLGLSLGGLLPSVNALLRRATPVHMVSRVYGYNNSFVSIGSMLGPMIGGFLAGYVSISG 372 Query: 176 IFYI 179 +FY+ Sbjct: 373 VFYM 376 >A0AM75.1 A0AM75_LISW6 Complete genome Length = 402 Score = 264 bits (674), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 146/357 (40%), Positives = 219/357 (61%), Gaps = 4/357 (1%) Query: 12 ETWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQG-DAIGLWAGLIFAANFMTSF 70 E+WK NL V+W G FL G+ +I PFL LY+ ++LGV D + LW+G+ ++ F+ S Sbjct: 2 ESWKKNLYVVWVGCFLTGTGLNLIMPFLPLYI-EELGVHNPDQVSLWSGIALSSTFLVSA 60 Query: 71 LFQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASI 130 + P+WGKLAD+ GR+IMLLR++ GMA+ ++LMG +Q G SG+ + Sbjct: 61 IMSPIWGKLADQKGRRIMLLRAALGMAIAMILMGLVSNVYQFVGLRLLMGIFSGYISTAN 120 Query: 131 ALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXX 190 AL++ P+ R G+A+G + + +V+G ++GPLIGGAL+D G RP+FYI G Sbjct: 121 ALIATQVPRHRSGWALGALSTAAVSGVLIGPLIGGALSDSFGVRPVFYITGVLLLGSFFL 180 Query: 191 XXXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYV 250 VKEKF E ++ S E F L K + +LF T ++Q A + +L LYV Sbjct: 181 TLFFVKEKFTPLEKQEMR--SGKEVFLSLEKPGLIVSLFITTMMIQIASNSVNPILTLYV 238 Query: 251 EKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQA 310 L G+A +IAF +G++++V GV+ ++A+P LG+ D++G+ RIL ALIG+ L IP A Sbjct: 239 RDLAGNAQNIAFISGMIASVPGVAALIAAPRLGRWGDRIGSERILLGALIGSMLLQIPMA 298 Query: 311 FVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 F + QL I+RFL+G+ G LLP+V +L+ TP R FG+N S +GN++G Sbjct: 299 FAQNPLQLGILRFLLGLTDGALLPAVQSLLTKNTPREVSGRIFGYNQSFQYIGNVIG 355 Score = 70.5 bits (171), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 48/164 (29%), Positives = 79/164 (48%), Gaps = 2/164 (1%) Query: 18 LIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLFQPLWG 77 ++ L+ ++ + P LTLY+ +DL I +G+I + + + + P G Sbjct: 213 IVSLFITTMMIQIASNSVNPILTLYV-RDLAGNAQNIAFISGMIASVPGVAALIAAPRLG 271 Query: 78 KLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISG-FNPASIALVSGN 136 + D+ G + +LL + G L+ + M FAQ P Q G G PA +L++ N Sbjct: 272 RWGDRIGSERILLGALIGSMLLQIPMAFAQNPLQLGILRFLLGLTDGALLPAVQSLLTKN 331 Query: 137 TPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIV 180 TP+ G G QS G ++GPLIG ++A GY +F +V Sbjct: 332 TPREVSGRIFGYNQSFQYIGNVIGPLIGSSVAAHFGYGDVFLVV 375 >B8GI60.1 B8GI60_METPE Major facilitator superfamily MFS_1 Length = 399 Score = 262 bits (670), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 147/394 (37%), Positives = 223/394 (56%), Gaps = 5/394 (1%) Query: 12 ETWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDA-IGLWAGLIFAANFMTSF 70 + WK NLIV WFG F+ G++ I P L LY+ + LGV A I ++G+ F F+ S Sbjct: 2 QNWKRNLIVCWFGMFMTGMGLSQIAPVLPLYI-QHLGVDNTALIEQFSGIAFGVTFIISA 60 Query: 71 LFQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASI 130 +F P+WG ADK+GRK MLLR+S GMA+VI MGFAQ +Q G I+G++ A Sbjct: 61 IFSPIWGLAADKFGRKSMLLRASLGMAIVIGCMGFAQNVYQLIGLRLLQGVITGYSSACT 120 Query: 131 ALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXX 190 AL++ T + G+A+G + + S+AGT+LGP+IGG +A+ +G++ +F+I G Sbjct: 121 ALIATQTDREHAGWALGTLSTSSIAGTLLGPMIGGYIAENLGFQEVFFITGALLLIAFIA 180 Query: 191 XXXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYV 250 V E F R + +S+ E + + LF +F++ A+ + +L +Y+ Sbjct: 181 TALFVNESFTRQDRM---VLSIKETWGTVPHKSLTLILFVSSFVMTLALYSVQPILTIYI 237 Query: 251 EKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQA 310 +L + +A AG+ + +G+++++A+ LGKLSDK+G +++ ALI A L IPQA Sbjct: 238 TQLSSTTSHVALLAGMTFSASGLASIVAASQLGKLSDKIGPQKVMLAALIVAGLIFIPQA 297 Query: 311 FVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLGAVX 370 FVT WQL+ +RF++G+ + GL+PSVN L++ TPD R FGFN S LG G+V Sbjct: 298 FVTDPWQLMALRFVLGLAIAGLIPSVNTLLKRITPDSLTGRVFGFNMSAGYLGVFGGSVL 357 Query: 371 XXXXXXXXXXXXXXXXXXXXXXXNTVWVRLKLYK 404 N VWV K+YK Sbjct: 358 GGQVAAYLGIRSVFFITGALLLVNAVWVYFKVYK 391 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,153,919,773 Number of extensions: 82657944 Number of successful extensions: 412689 Number of sequences better than 1.0e-05: 2874 Number of HSP's gapped: 413128 Number of HSP's successfully gapped: 3392 Length of query: 412 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 274 Effective length of database: 1,760,596,133 Effective search space: 482403340442 Effective search space used: 482403340442 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)