BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0002.p (321 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLB1.1 B3KLB1_9BACI Ferrochelatase EC=4.99.1.1 479 e-133 B1DL25.1 B1DL25_9BACL Ferrochelatase EC=4.99.1.1 420 e-115 C1PSD3.1 C1PSD3_ALIAC Ferrochelatase 301 4e-80 B7DNV2.1 B7DNV2_9BACL Ferrochelatase EC=4.99.1.1 298 6e-79 B1YKJ8.1 B1YKJ8_EXIS2 Ferrochelatase EC=4.99.1.1 275 5e-72 >B3KLB1.1 B3KLB1_9BACI Ferrochelatase EC=4.99.1.1 Length = 313 Score = 479 bits (1232), Expect = e-133, Method: Compositional matrix adjust. Identities = 228/312 (73%), Positives = 265/312 (84%), Gaps = 1/312 (0%) Query: 4 VTTKIGVLVMSYGTPESLEGVEAYYTHIRRGHAPSPEQLKELKDRYEAIVGGVFPLRENT 63 + KIGVLVMSYGTPES+EG+E+YYTHIRRG+ PS EQLKEL DRYEAIVGGVFPLRENT Sbjct: 1 MKNKIGVLVMSYGTPESMEGIESYYTHIRRGNKPSEEQLKELTDRYEAIVGGVFPLRENT 60 Query: 64 NRQVEALQAALNNGHAGEKVEFVCYQGLKHARPFIEDGVEAMAKDGITHGVGIVLAPHYS 123 +RQV LQ LN +EFVCYQGLKHA P+IEDGVE M +DGI VGIVLAPHYS Sbjct: 61 DRQVRTLQDTLNADPRAIDLEFVCYQGLKHAAPYIEDGVEQMVRDGIKEAVGIVLAPHYS 120 Query: 124 VMSVGTYIKRAKEKAEASGIEMAFVESYHLHPELIEVLSRRVSAKLDLFEEAGAVREDVR 183 MS+G+Y+KRA+ KA+ G++++FVESYH+HP+LIE + RVSAKL+ FE+AGAVR+ VR Sbjct: 121 TMSIGSYVKRAQAKADELGLQVSFVESYHMHPKLIEAFADRVSAKLNQFEQAGAVRDSVR 180 Query: 184 VLFSAHSLPERILSMGDPYRDQLLATSEAVAKQAGVRNWQFTWQSAGRTAEPWLGPDILE 243 VLFSAHSLPERILS+GDPY+DQLL TS+AVA++AGV+ WQFTWQSAGRTAEPWLGPDIL+ Sbjct: 181 VLFSAHSLPERILSVGDPYQDQLLETSKAVAEKAGVKQWQFTWQSAGRTAEPWLGPDILD 240 Query: 244 TLHELS-EAQVKYVLVAPVGFVSDHLEVLYDLDIEAQALASELDMRLMRIDSLNSDPAYM 302 TL L+ E QV+ VLVAPVGFVSDHLEVLYDLDIEA+++A E+DMRL RIDSLNSDP YM Sbjct: 241 TLQTLNKEEQVEDVLVAPVGFVSDHLEVLYDLDIEAKSIAKEMDMRLERIDSLNSDPLYM 300 Query: 303 SVLSDVVRTAAG 314 LSD V T G Sbjct: 301 EALSDSVITLIG 312 >B1DL25.1 B1DL25_9BACL Ferrochelatase EC=4.99.1.1 Length = 315 Score = 420 bits (1079), Expect = e-115, Method: Compositional matrix adjust. Identities = 208/315 (66%), Positives = 242/315 (76%), Gaps = 5/315 (1%) Query: 1 MESVTTKIGVLVMSYGTPESLEGVEAYYTHIRRGHAPSPEQLKELKDRYEAIVGGVFPLR 60 M + KIGVLVMSYGTP+SL+ VEAYYTHIRRGHAP+PEQLKEL DRYEAIVGGVFPLR Sbjct: 1 MSEMNNKIGVLVMSYGTPQSLDDVEAYYTHIRRGHAPTPEQLKELTDRYEAIVGGVFPLR 60 Query: 61 ENTNRQVEALQAALNNGHAGEKVEFVCYQGLKHARPFIEDGVEAMAKDGITHGVGIVLAP 120 ENTN QV LQ L G +VCYQGLKHA P+IEDGVE MA+DGI VGIVLAP Sbjct: 61 ENTNGQVAGLQDKLEQLAPGR---YVCYQGLKHAVPYIEDGVEQMARDGIKEAVGIVLAP 117 Query: 121 HYSVMSVGTYIKRAKEKAEASGIEMAFVESYHLHPELIEVLSRRVSAKLDLFEEAGAVRE 180 HYS+MSVG+Y KRA+EKA GI+M+FV+ YHLHP+L++ L+ RV+ L E+ Sbjct: 118 HYSIMSVGSYNKRAQEKAAELGIKMSFVKEYHLHPKLLQALNERVTDGLQRLSESSGNAA 177 Query: 181 DVRVLFSAHSLPERILSMGDPYRDQLLATSEAVAKQAGV--RNWQFTWQSAGRTAEPWLG 238 V+VLFSAHSLPE+I MGDPY QLLATS+AVAK A V ++WQFTWQSAGRT EPWLG Sbjct: 178 PVKVLFSAHSLPEKIREMGDPYEHQLLATSDAVAKLANVDAQDWQFTWQSAGRTREPWLG 237 Query: 239 PDILETLHELSEAQVKYVLVAPVGFVSDHLEVLYDLDIEAQALASELDMRLMRIDSLNSD 298 PDIL+TL L++ +K VLVAPVGFVSDHLEVLYDLD+EA+ A+EL + L RI LN D Sbjct: 238 PDILDTLKVLTDEGIKAVLVAPVGFVSDHLEVLYDLDLEAKQFAAELGITLERITMLNKD 297 Query: 299 PAYMSVLSDVVRTAA 313 P YM L++ V A Sbjct: 298 PLYMETLAESVMNAG 312 >C1PSD3.1 C1PSD3_ALIAC Ferrochelatase Length = 306 Score = 301 bits (772), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 156/307 (50%), Positives = 204/307 (66%), Gaps = 8/307 (2%) Query: 6 TKIGVLVMSYGTPESLEGVEAYYTHIRRGHAPSPEQLKELKDRYEAIVGGVFPLRENTNR 65 T +GVL+M+YGTP SL+ VEAYYT IRRG PSP +L +L RYEAI GGV PLRE T R Sbjct: 2 TTLGVLMMAYGTPRSLDEVEAYYTDIRRGRKPSPAELADLVRRYEAI-GGVSPLREITER 60 Query: 66 QVEALQAALNNGHAGEKVEFVCYQGLKHARPFIEDGVEAMAKDGITHGVGIVLAPHYSVM 125 Q +A++ LN A + F Y G+KH P I D V M +DGI + +VLAPHYS M Sbjct: 61 QAKAVEHVLN---AQGETAFRVYLGMKHTEPRIADAVADMRRDGIERAIALVLAPHYSAM 117 Query: 126 SVGTYIKRAKEKAEASGIEMAFVESYHLHPELIEVLSRRVSAKLDLFEEAGAVREDVRVL 185 SVGTY ++A+E A G ++ V +HL P ++ L+ RV L L + E V+ Sbjct: 118 SVGTYHEQAREAARDGGPKLYCVNQWHLEPRFLDALASRVEEALRLCKSP----EQAMVI 173 Query: 186 FSAHSLPERILSMGDPYRDQLLATSEAVAKQAGVRNWQFTWQSAGRTAEPWLGPDILETL 245 F+AHSLP RIL MGDPY DQL + EAVAK+ G+ ++ F WQSAGRT EPWLGPD+LE L Sbjct: 174 FTAHSLPARILDMGDPYVDQLRESGEAVAKRLGLAHYTFGWQSAGRTQEPWLGPDLLEIL 233 Query: 246 HELSEAQVKYVLVAPVGFVSDHLEVLYDLDIEAQALASELDMRLMRIDSLNSDPAYMSVL 305 L++ + V+V GFV+DHLEVLYD+DIEA+ A EL ++L+R +N DP ++ + Sbjct: 234 ESLAKEGYREVVVCSQGFVADHLEVLYDIDIEAKQRAEELGLKLVRTRQMNDDPDFVRAV 293 Query: 306 SDVVRTA 312 +DV+ A Sbjct: 294 ADVIERA 300 >B7DNV2.1 B7DNV2_9BACL Ferrochelatase EC=4.99.1.1 Length = 306 Score = 298 bits (762), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 155/307 (50%), Positives = 203/307 (66%), Gaps = 8/307 (2%) Query: 6 TKIGVLVMSYGTPESLEGVEAYYTHIRRGHAPSPEQLKELKDRYEAIVGGVFPLRENTNR 65 T +GVL+M+YGTP SL+ VEAYYT IRRG PSP +L +L RYEAI GGV PLRE T R Sbjct: 2 TTLGVLMMAYGTPRSLDEVEAYYTDIRRGRKPSPAELADLVRRYEAI-GGVSPLREITER 60 Query: 66 QVEALQAALNNGHAGEKVEFVCYQGLKHARPFIEDGVEAMAKDGITHGVGIVLAPHYSVM 125 Q +A++ LN+ GE F Y G+KH P + D V M +DGI + +VLAPHYS M Sbjct: 61 QAKAVEQILNS--QGETA-FRVYLGMKHTEPRVADAVADMRRDGIERAIALVLAPHYSAM 117 Query: 126 SVGTYIKRAKEKAEASGIEMAFVESYHLHPELIEVLSRRVSAKLDLFEEAGAVREDVRVL 185 SV TY K+A+E A G ++ V +HL P ++ L+ RV L L + E V+ Sbjct: 118 SVETYHKQAREAARDGGPKLYCVNQWHLEPRFLDALAARVEEALRLCDSP----ERAMVI 173 Query: 186 FSAHSLPERILSMGDPYRDQLLATSEAVAKQAGVRNWQFTWQSAGRTAEPWLGPDILETL 245 F+AHSLP RIL MGDPY DQL + EAVAK+ G+ ++ F WQSAGRT EPWLGPD+L+ L Sbjct: 174 FTAHSLPARILEMGDPYVDQLRESGEAVAKRLGLAHYTFGWQSAGRTQEPWLGPDLLDML 233 Query: 246 HELSEAQVKYVLVAPVGFVSDHLEVLYDLDIEAQALASELDMRLMRIDSLNSDPAYMSVL 305 L++ + V+V GFV+DHLEVLYD+DIEA+ A EL ++L R +N DP ++ + Sbjct: 234 ESLAKQGYREVVVCSQGFVADHLEVLYDIDIEAKQRAEELGLKLFRTRQMNDDPDFVRAV 293 Query: 306 SDVVRTA 312 +DV+ A Sbjct: 294 ADVIERA 300 >B1YKJ8.1 B1YKJ8_EXIS2 Ferrochelatase EC=4.99.1.1 Length = 310 Score = 275 bits (702), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 151/311 (48%), Positives = 196/311 (63%), Gaps = 8/311 (2%) Query: 8 IGVLVMSYGTPESLEGVEAYYTHIRRGHAPSPEQLKELKDRYEAIVGGVFPLRENTNRQV 67 +G+LVM+YGTP E +E YYTHIRRG PSPE L EL +RYEAI GGV PL + T Q Sbjct: 4 LGLLVMAYGTPYKPEDIERYYTHIRRGRKPSPEALAELTERYEAI-GGVSPLAQVTIDQA 62 Query: 68 EALQAALNNGHAGEKVEFVCYQGLKHARPFIEDGVEAMAKDGITHGVGIVLAPHYSVMSV 127 E L L +AGE +EF Y GLKH PF+ED VE MAKDGIT V +VLAPHYS S+ Sbjct: 63 EELHRQLTEKYAGE-IEFKLYLGLKHIEPFVEDAVEQMAKDGITEAVTVVLAPHYSTFSI 121 Query: 128 GTYIKRAKEKAEASGIEMAFVESYHLHPELIEVLSRRVSAKLDLFEEAGAVREDVRVL-F 186 +Y RAKE A+ GI +A VES++ I+ + D A V ED VL Sbjct: 122 RSYNGRAKEVADKHGIHLASVESWYKEEAFIDWWGTEIRKTFD----ALPVPEDRAVLVV 177 Query: 187 SAHSLPERILSMGDPYRDQLLATSEAVAKQAGVRNWQFTWQSAGRTAEPWLGPDILETLH 246 SAHSLPE+I++ GDPY +QL T++ +A +AGV++ WQSAG+T EPWLGPD+ + Sbjct: 178 SAHSLPEKIIANGDPYPEQLKETADLIAARAGVKHMITGWQSAGQTPEPWLGPDVQDLTR 237 Query: 247 ELSEAQVKYVLV-APVGFVSDHLEVLYDLDIEAQALASELDMRLMRIDSLNSDPAYMSVL 305 ++ EA+ V PVGFV+DHLEVL+D D E + + EL + R N+DPA+M + Sbjct: 238 DVYEAEGYEAFVYVPVGFVADHLEVLFDNDYECKVVCDELGIHYARPIMPNADPAFMKAV 297 Query: 306 SDVVRTAAGQF 316 ++ V G + Sbjct: 298 TEAVSKQVGSY 308 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,775,047,471 Number of extensions: 71306413 Number of successful extensions: 207878 Number of sequences better than 1.0e-05: 1316 Number of HSP's gapped: 205118 Number of HSP's successfully gapped: 1318 Length of query: 321 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 186 Effective length of database: 1,788,859,178 Effective search space: 332727807108 Effective search space used: 332727807108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)