BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0001.p (496 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DL24.1 B1DL24_9BACL Protoporphyrinogen oxidase EC=1.3.3.4 508 e-142 Q5L282.1 Q5L282_GEOKA Protoporphyrinogen IX /coproporphyrinogen ... 395 e-108 C3B073.1 C3B073_BACMY Protoporphyrinogen oxidase 394 e-108 C3AIG6.1 C3AIG6_BACMY Protoporphyrinogen oxidase 394 e-108 C3J2N2.1 C3J2N2_9BACI Protoporphyrinogen oxidase EC=1.3.3.4 394 e-107 >B1DL24.1 B1DL24_9BACL Protoporphyrinogen oxidase EC=1.3.3.4 Length = 477 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 252/479 (52%), Positives = 331/479 (69%), Gaps = 19/479 (3%) Query: 7 KVVIIGGGLSGLSAAFYVRKYYREAGIQPDIILVEKDKSLGGKIETLHREGFVIEKGPDS 66 ++V+IGGG+SGLS+AFY+++ G Q + +V+ LGGKI TL R+GFVIEKGPDS Sbjct: 4 RIVVIGGGISGLSSAFYLQREAERQGKQVKLTIVDGAPVLGGKINTLQRDGFVIEKGPDS 63 Query: 67 FLARKTVMSELAKELEIDHELVTTNPNAKKTYILQRGKLHPMPAGLVLGIPTELKPFLKS 126 FL+RK + ELAKEL I++EL N N K +YI++ GKLHPMP G+VLGIPT L F+++ Sbjct: 64 FLSRKLPIIELAKELGIENELTAQNENGKTSYIMKGGKLHPMPKGMVLGIPTNLDTFMQT 123 Query: 127 GLVTFGGKMRAMMDFIIPPRRSTEDESLGALIERRLGTEVLENMTEPLLAGIYAGDMRKI 186 L++ GK R+++D +P DESLG + RR+G E++++++EPLLAGIYAGD+ K+ Sbjct: 124 TLLSDEGKARSLLDLEMPRAAPEGDESLGDFLSRRIGAEIVKHISEPLLAGIYAGDLSKL 183 Query: 187 SLQATFPQFGDVERKHGSLIRGMMMGRKPAETHTG-------TKKSAFLTFRKGLQSLVH 239 S+QATFPQF + ER++GSLIRG M +K H K S FL+F++GL ++VH Sbjct: 184 SIQATFPQFAEAERQYGSLIRGTQMQQKNKAPHQAPDYLPEELKNSVFLSFKEGLSTMVH 243 Query: 240 ALIHELHDVEQRXXXXXXXXXXXXXXXXXXXXSQVAGTGPSPRYEIELDNGELVPADDVY 299 AL L VE+R + + G + YE+ L++GE+VPAD V Sbjct: 244 ALDQALQSVERRLGDAVVAI------------NPLNGEDAASNYEVVLESGEVVPADHVL 291 Query: 300 VTVQNFAAADLLRPHVDVSALDSVNYVSVANVVMAFAKKDIHTEYDGSGFLVPRKEGRNI 359 VTV F AADLL P VDV+ L ++NYVSVANVVMAF K +DGSGFLVPR EG I Sbjct: 292 VTVPAFHAADLLEPLVDVTELRAINYVSVANVVMAFEKSTFDRAFDGSGFLVPRSEGLRI 351 Query: 360 TACTWTSTKWLHTSPDDRVLLRCYVGRSGDEQNVELPDAALTELVLKDLKEIMGITAQPM 419 TACTWTS KWLHTSP+D+VLLRCYVGR+GDE+ LPD L + VL+D+++++GI A P+ Sbjct: 352 TACTWTSNKWLHTSPEDKVLLRCYVGRAGDEEVAMLPDDELKKAVLEDIQKVLGIDAVPV 411 Query: 420 FTEITRLKHSMPQYPVGHPARIAALRSELTQKLPGVYAFGAGYDGIGMPDCIKQAKLTA 478 FTEITRL SMPQYPVGH AALR L ++LPGVY GA + G+G+PDCI+ K A Sbjct: 412 FTEITRLPRSMPQYPVGHLENTAALRDRLAEELPGVYVTGAAFGGVGLPDCIRSGKEAA 470 >Q5L282.1 Q5L282_GEOKA Protoporphyrinogen IX /coproporphyrinogen III oxidase Length = 479 Score = 395 bits (1015), Expect = e-108, Method: Compositional matrix adjust. Identities = 211/485 (43%), Positives = 292/485 (60%), Gaps = 24/485 (4%) Query: 6 RKVVIIGGGLSGLSAAFYVRKYYREAGIQPDIILVEKDKSLGGKIETLHREGFVIEKGPD 65 R VVIIGGG++GL+AA+Y++K RE + LVE LGGK++T+ R+GFVIE+GPD Sbjct: 6 RTVVIIGGGITGLTAAYYLQKAIREQRLPLACKLVEATHRLGGKVQTVVRDGFVIERGPD 65 Query: 66 SFLARKTVMSELAKELEIDHELVTTNPNAKKT-YILQRGKLHPMPAGLVLGIPTELKPFL 124 SFLARKT L KE+ ++HE+V NA T YIL GKL+P+P G V+GIPT + PFL Sbjct: 66 SFLARKTSAFRLVKEVGLEHEIVH---NATGTSYILVNGKLYPIPGGAVMGIPTRIAPFL 122 Query: 125 KSGLVTFGGKMRAMMDFIIPPRRSTEDESLGALIERRLGTEVLENMTEPLLAGIYAGDMR 184 +GL + GK+RA +DF++PP + D LG RRLG EV++N+ EPLL+GIYAGD+ Sbjct: 123 TTGLFSPAGKLRAALDFVLPPVKVDGDMPLGRFFRRRLGDEVVDNLIEPLLSGIYAGDID 182 Query: 185 KISLQATFPQFGDVERKHGSLIRGMMMGRKPAETHTGTKKSAFLTFRKGLQSLVHALIHE 244 ++SL ATFPQF +E+K+GSL+RG R+ +K AF T + GLQSLV Sbjct: 183 EMSLMATFPQFFQLEQKYGSLVRG---ARRTTPKEQKERKGAFQTLKTGLQSLVD----- 234 Query: 245 LHDVEQRXXXXXXXXXXXXXXXXXXXXSQVAGTGPSPRYEIELDNGELVPADDVYVTVQN 304 +VE+R + Y ++L GE+ AD + VT + Sbjct: 235 --EVEKRLEPGSVIKGVRVERIVRTDSA----------YRLQLSTGEVWGADGIIVTTPH 282 Query: 305 FAAADLLRPHVDVSALDSVNYVSVANVVMAFAKKDIHTEYDGSGFLVPRKEGRNITACTW 364 D+ + + SV SVA V +AF ++ I + DG+GF+V R+ +TACTW Sbjct: 283 HVVPDMFADYPFFAPFRSVPLTSVATVALAFPEEAIRQDIDGTGFVVSRRSDYTMTACTW 342 Query: 365 TSTKWLHTSPDDRVLLRCYVGRSGDEQNVELPDAALTELVLKDLKEIMGITAQPMFTEIT 424 T KW HT+P + LLRCYVGR GDE+ V+ PD + +VL DL ++M I QP F I+ Sbjct: 343 THKKWPHTAPPGKALLRCYVGRPGDEEIVDQPDDEIVRVVLDDLNKVMNIAGQPEFAVIS 402 Query: 425 RLKHSMPQYPVGHPARIAALRSELTQKLPGVYAFGAGYDGIGMPDCIKQAKLTAESAAGR 484 R K +MPQY VGH R+A ++ ++ LPGV+ G+ Y+G+G+PDCI Q + Sbjct: 403 RWKRAMPQYTVGHRERLAHIKRQMGDALPGVFLAGSSYEGLGLPDCIDQGEKAVRDVLLY 462 Query: 485 LVQQP 489 L Q P Sbjct: 463 LQQAP 467 >C3B073.1 C3B073_BACMY Protoporphyrinogen oxidase Length = 473 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 205/470 (43%), Positives = 290/470 (61%), Gaps = 20/470 (4%) Query: 6 RKVVIIGGGLSGLSAAFYVRKYYREAGIQPDIILVEKDKSLGGKIETLHREGFVIEKGPD 65 +KVVIIGGG++GL+ A+Y++K RE G+ D +L+E LGGKI+T+ ++GF IE+GPD Sbjct: 3 KKVVIIGGGITGLTTAYYLQKEIREKGLPIDTLLIEASGKLGGKIQTVRKDGFTIERGPD 62 Query: 66 SFLARKTVMSELAKELEIDHELVTTNPNAKKTYILQRGKLHPMPAGLVLGIPTELKPFLK 125 SFL RK + LA+ELE+ ELV N ++++L +LH MP+G ++GIPT++ PFL Sbjct: 63 SFLERKESAARLARELELGDELV--NNATGQSFVLVNNRLHKMPSGSMMGIPTQVTPFLF 120 Query: 126 SGLVTFGGKMRAMMDFIIPPRRSTEDESLGALIERRLGTEVLENMTEPLLAGIYAGDMRK 185 SGL + GK+RA DF++P + D+SLG RRLG EV+EN+ EPLL+GIYAGD+ + Sbjct: 121 SGLFSPIGKVRAGFDFVLPRSKPVSDQSLGQFFRRRLGNEVVENLIEPLLSGIYAGDIDQ 180 Query: 186 ISLQATFPQFGDVERKHGSLIRGMMMGRKPAETHTGTKKSAFLTFRKGLQSLVHALIHEL 245 +SL ATFPQF VE+K+ S+ GM P + K FLT + GLQS+V + +L Sbjct: 181 MSLMATFPQFYQVEQKYRSISLGMRT-LAPKKIKDVKPKGIFLTLKTGLQSIVEKIEEQL 239 Query: 246 HDVEQRXXXXXXXXXXXXXXXXXXXXSQVAGTGPSPRYEIELDNGELVPADDVYVTVQNF 305 D +VA G Y I L NG+ + AD + V + Sbjct: 240 ED---------------GTVVKGTRIEKVAKMGDG--YHITLSNGKEIEADSIVVAASHK 282 Query: 306 AAADLLRPHVDVSALDSVNYVSVANVVMAFAKKDIHTEYDGSGFLVPRKEGRNITACTWT 365 + + + ++ SVANVV+AF K+ I + DG+GF+V R +ITACTWT Sbjct: 283 VLPSMFAQYKEFRFFRNIPSTSVANVVLAFPKEAIKRDIDGTGFVVSRNSDFSITACTWT 342 Query: 366 STKWLHTSPDDRVLLRCYVGRSGDEQNVELPDAALTELVLKDLKEIMGITAQPMFTEITR 425 KW HT+P+ +VLLRCYVGR GDE VE D + + +L+DL++ M I A P FT ++R Sbjct: 343 HKKWPHTTPEGKVLLRCYVGRPGDEAIVEQTDEEIVQFILEDLQKTMDIKADPDFTVVSR 402 Query: 426 LKHSMPQYPVGHPARIAALRSELTQKLPGVYAFGAGYDGIGMPDCIKQAK 475 K +MPQY VGH R+ L++ + ++LPGVY G+ Y G G+PDCI Q + Sbjct: 403 WKDAMPQYTVGHKERMTKLKTFMDKELPGVYLAGSSYAGSGLPDCIDQGE 452 >C3AIG6.1 C3AIG6_BACMY Protoporphyrinogen oxidase Length = 473 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 205/470 (43%), Positives = 290/470 (61%), Gaps = 20/470 (4%) Query: 6 RKVVIIGGGLSGLSAAFYVRKYYREAGIQPDIILVEKDKSLGGKIETLHREGFVIEKGPD 65 +KVVIIGGG++GL+ A+Y++K RE G+ D +L+E LGGKI+T+ ++GF IE+GPD Sbjct: 3 KKVVIIGGGITGLTTAYYLQKEIREKGLPIDTLLIEASGKLGGKIQTVRKDGFTIERGPD 62 Query: 66 SFLARKTVMSELAKELEIDHELVTTNPNAKKTYILQRGKLHPMPAGLVLGIPTELKPFLK 125 SFL RK + LA+ELE+ ELV N ++++L +LH MP+G ++GIPT++ PFL Sbjct: 63 SFLERKESAARLARELELGDELV--NNATGQSFVLVNNRLHKMPSGSMMGIPTQVTPFLF 120 Query: 126 SGLVTFGGKMRAMMDFIIPPRRSTEDESLGALIERRLGTEVLENMTEPLLAGIYAGDMRK 185 SGL + GK+RA DF++P + D+SLG RRLG EV+EN+ EPLL+GIYAGD+ + Sbjct: 121 SGLFSPIGKVRAGFDFVLPRSKPVSDQSLGQFFRRRLGNEVVENLIEPLLSGIYAGDIDQ 180 Query: 186 ISLQATFPQFGDVERKHGSLIRGMMMGRKPAETHTGTKKSAFLTFRKGLQSLVHALIHEL 245 +SL ATFPQF VE+K+ S+ GM P + K FLT + GLQS+V + +L Sbjct: 181 MSLMATFPQFYQVEQKYRSISLGMRT-LAPKKIKDVKPKGIFLTLKTGLQSIVEKIEEQL 239 Query: 246 HDVEQRXXXXXXXXXXXXXXXXXXXXSQVAGTGPSPRYEIELDNGELVPADDVYVTVQNF 305 D +VA G Y I L NG+ + AD + V + Sbjct: 240 ED---------------GTVVKGTRIEKVAKMGDG--YHITLSNGKEIEADSIVVAASHK 282 Query: 306 AAADLLRPHVDVSALDSVNYVSVANVVMAFAKKDIHTEYDGSGFLVPRKEGRNITACTWT 365 + + + ++ SVANVV+AF K+ I + DG+GF+V R +ITACTWT Sbjct: 283 VLPSMFAQYKEFRFFRNIPSTSVANVVLAFPKEAIKRDIDGTGFVVSRNSDFSITACTWT 342 Query: 366 STKWLHTSPDDRVLLRCYVGRSGDEQNVELPDAALTELVLKDLKEIMGITAQPMFTEITR 425 KW HT+P+ +VLLRCYVGR GDE VE D + + +L+DL++ M I A P FT ++R Sbjct: 343 HKKWPHTTPEGKVLLRCYVGRPGDEAIVEQTDEEIVQFILEDLQKTMDIKADPDFTVVSR 402 Query: 426 LKHSMPQYPVGHPARIAALRSELTQKLPGVYAFGAGYDGIGMPDCIKQAK 475 K +MPQY VGH R+ L++ + ++LPGVY G+ Y G G+PDCI Q + Sbjct: 403 WKDAMPQYTVGHKERMTKLKTFMDKELPGVYLAGSSYAGSGLPDCIDQGE 452 >C3J2N2.1 C3J2N2_9BACI Protoporphyrinogen oxidase EC=1.3.3.4 Length = 479 Score = 394 bits (1012), Expect = e-107, Method: Compositional matrix adjust. Identities = 210/485 (43%), Positives = 292/485 (60%), Gaps = 24/485 (4%) Query: 6 RKVVIIGGGLSGLSAAFYVRKYYREAGIQPDIILVEKDKSLGGKIETLHREGFVIEKGPD 65 R VVIIGGG++GL+AA+Y++K RE + LVE LGGK++T+ R+GFVIE+GPD Sbjct: 6 RTVVIIGGGITGLTAAYYLQKAIREQRLPLACKLVEATHRLGGKVQTVVRDGFVIERGPD 65 Query: 66 SFLARKTVMSELAKELEIDHELVTTNPNAKKT-YILQRGKLHPMPAGLVLGIPTELKPFL 124 SFLARKT L KE+ ++HE+V NA T YIL GKL+P+P G V+GIPT + PFL Sbjct: 66 SFLARKTSAFRLVKEVGLEHEIVH---NATGTSYILVNGKLYPIPGGAVMGIPTRIAPFL 122 Query: 125 KSGLVTFGGKMRAMMDFIIPPRRSTEDESLGALIERRLGTEVLENMTEPLLAGIYAGDMR 184 +GL + GK+RA +DF++PP + D LG RRLG EV++N+ EPLL+GIYAGD+ Sbjct: 123 TTGLFSPAGKLRAALDFVLPPVKVDGDMPLGRFFRRRLGDEVVDNLIEPLLSGIYAGDID 182 Query: 185 KISLQATFPQFGDVERKHGSLIRGMMMGRKPAETHTGTKKSAFLTFRKGLQSLVHALIHE 244 ++SL ATFPQF +E+K+GSL+RG R+ +K AF T + GLQSLV Sbjct: 183 EMSLMATFPQFFQLEQKYGSLVRG---ARRTTPKEQKERKGAFQTLKTGLQSLVD----- 234 Query: 245 LHDVEQRXXXXXXXXXXXXXXXXXXXXSQVAGTGPSPRYEIELDNGELVPADDVYVTVQN 304 +VE+R + Y ++L G++ AD + VT + Sbjct: 235 --EVEKRLEPGSVIKGVRVERIVRTDSA----------YRLQLSTGDVWGADGIIVTTPH 282 Query: 305 FAAADLLRPHVDVSALDSVNYVSVANVVMAFAKKDIHTEYDGSGFLVPRKEGRNITACTW 364 D+ + + SV SVA V +AF ++ I + DG+GF+V R+ +TACTW Sbjct: 283 HVVPDMFADYPFFAPFRSVPLTSVATVALAFPEEAIRQDIDGTGFVVSRRSDYTMTACTW 342 Query: 365 TSTKWLHTSPDDRVLLRCYVGRSGDEQNVELPDAALTELVLKDLKEIMGITAQPMFTEIT 424 T KW HT+P + LLRCYVGR GDE+ V+ PD + +VL DL ++M I QP F I+ Sbjct: 343 THKKWPHTAPPGKALLRCYVGRPGDEEIVDQPDDEIVRVVLDDLNKVMNIAGQPEFAVIS 402 Query: 425 RLKHSMPQYPVGHPARIAALRSELTQKLPGVYAFGAGYDGIGMPDCIKQAKLTAESAAGR 484 R K +MPQY VGH R+A ++ ++ LPGV+ G+ Y+G+G+PDCI Q + Sbjct: 403 RWKRAMPQYTVGHRERLAHIKRQMGDALPGVFLAGSSYEGLGLPDCIDQGEKAVRDVLLY 462 Query: 485 LVQQP 489 L Q P Sbjct: 463 LQQAP 467 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,888,008,158 Number of extensions: 123331200 Number of successful extensions: 308367 Number of sequences better than 1.0e-05: 550 Number of HSP's gapped: 307120 Number of HSP's successfully gapped: 792 Length of query: 496 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 356 Effective length of database: 1,741,754,103 Effective search space: 620064460668 Effective search space used: 620064460668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)