BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0011.p (159 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DD20.1 B1DD20_9BACL Elongation factor G domain protein 270 3e-71 Q1VQ31.1 Q1VQ31_9FLAO Tetracycline resistance protein 138 2e-31 Q97J38.1 Q97J38_CLOAB Tetracycline resistance protein, tetQ fami... 121 2e-26 Q1PLK8.1 Q1PLK8_CLOPE Ribosomal protection-like tetracycline res... 121 2e-26 C1IBC5.1 C1IBC5_9CLOT Tetracycline resistance protein 121 2e-26 >B1DD20.1 B1DD20_9BACL Elongation factor G domain protein Length = 379 Score = 270 bits (691), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 126/158 (79%), Positives = 140/158 (88%) Query: 2 QGLYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVV 61 QGL GWEV DLKVTL EGQHHVWHTHPLDF VATPMAIM+GL+ VGTKLLEP+LQ RIVV Sbjct: 222 QGLKGWEVVDLKVTLIEGQHHVWHTHPLDFVVATPMAIMNGLDSVGTKLLEPVLQFRIVV 281 Query: 62 PEENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSF 121 PEENGGRVMND+V MRGTFE P LQG+R++IEGR+P ATSL+Y + L+S TKGR T +F Sbjct: 282 PEENGGRVMNDIVLMRGTFEAPSLQGDRLLIEGRVPAATSLEYSMELNSLTKGRGTMATF 341 Query: 122 FAGYEECPPDVRAERTRRGVNPLDQARYILSVRKALQG 159 FAGYEECPPD AERTRRGVNPLDQ++YILSVRKALQG Sbjct: 342 FAGYEECPPDAAAERTRRGVNPLDQSKYILSVRKALQG 379 >Q1VQ31.1 Q1VQ31_9FLAO Tetracycline resistance protein Length = 660 Score = 138 bits (347), Expect = 2e-31, Method: Composition-based stats. Identities = 69/159 (43%), Positives = 97/159 (61%) Query: 1 MQGLYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIV 60 +QG+ GWEVTD+K+TL +G+ H H+ P DF +ATPM IM GL GT LLEPIL I Sbjct: 497 VQGILGWEVTDVKITLIKGEDHNVHSRPGDFNLATPMGIMQGLKIGGTHLLEPILSFEIK 556 Query: 61 VPEENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTS 120 E G+++++L R FE P + + G +P+ATSLD+ + L++ T G+ Sbjct: 557 ANEALLGKIVSELSTRRANFETPQFVDDTFRLRGTIPVATSLDFSIKLNAITSGKLRLKL 616 Query: 121 FFAGYEECPPDVRAERTRRGVNPLDQARYILSVRKALQG 159 F GY+ CP A R +GVNPLD+A++IL R A + Sbjct: 617 QFYGYDTCPKGEGAIREYKGVNPLDEAQWILHRRGAYKA 655 >Q97J38.1 Q97J38_CLOAB Tetracycline resistance protein, tetQ family, GTPase Length = 652 Score = 121 bits (304), Expect = 2e-26, Method: Composition-based stats. Identities = 59/157 (37%), Positives = 91/157 (57%) Query: 2 QGLYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVV 61 QGL+GWEVTD KVTLT G+ + P DF TPM M+ L R T LLEP+ + + + Sbjct: 495 QGLFGWEVTDAKVTLTCGEFFSPASTPSDFRNVTPMVFMEALYRAQTVLLEPLYEFELRI 554 Query: 62 PEENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSF 121 P++ + + DL MR F+ P++ G+ I G +P+ S +Y + ++SYTKG+ FT+ Sbjct: 555 PQDVLSKAIWDLETMRAIFDSPIVIGDEFSIRGLIPVENSKEYKMKIASYTKGKGMFTTK 614 Query: 122 FAGYEECPPDVRAERTRRGVNPLDQARYILSVRKALQ 158 F GY+E R + +PL++ Y+L A++ Sbjct: 615 FYGYKEVSAKFAKARPKTTYDPLNKKEYLLYKLNAIR 651 >Q1PLK8.1 Q1PLK8_CLOPE Ribosomal protection-like tetracycline resistance protein Length = 652 Score = 121 bits (304), Expect = 2e-26, Method: Composition-based stats. Identities = 57/157 (36%), Positives = 93/157 (59%) Query: 2 QGLYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVV 61 QGL+GWEVTD+KVTL+ G+ + P DF TPM M+ L + T LLEP+ + + + Sbjct: 495 QGLFGWEVTDVKVTLSCGEFFSPASTPADFRNVTPMVFMEALYKAQTVLLEPLHEFELKI 554 Query: 62 PEENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSF 121 P+ + + DL MR TF+ P++ G+ I+G +P+ S +Y + ++SYT+GR F + Sbjct: 555 PQNALSKAVWDLETMRATFDNPIVIGDEFSIKGLIPVENSKEYKMKIASYTEGRGMFVTK 614 Query: 122 FAGYEECPPDVRAERTRRGVNPLDQARYILSVRKALQ 158 F GY+E + R + +PL++ Y+L A++ Sbjct: 615 FYGYKEASAEFSKARKKTTYDPLNKKEYLLHKLNAIR 651 >C1IBC5.1 C1IBC5_9CLOT Tetracycline resistance protein Length = 652 Score = 121 bits (304), Expect = 2e-26, Method: Composition-based stats. Identities = 57/157 (36%), Positives = 93/157 (59%) Query: 2 QGLYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVV 61 QGL+GWEVTD+KVTL+ G+ + P DF TPM M+ L + T LLEP+ + + + Sbjct: 495 QGLFGWEVTDVKVTLSCGEFFSPASTPADFRNVTPMVFMEALYKAQTVLLEPLHEFELKI 554 Query: 62 PEENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSF 121 P+ + + DL MR TF+ P++ G+ I+G +P+ S +Y + ++SYT+GR F + Sbjct: 555 PQNALSKAVWDLETMRATFDNPIVIGDEFSIKGLIPVENSKEYKMKIASYTEGRGMFVTK 614 Query: 122 FAGYEECPPDVRAERTRRGVNPLDQARYILSVRKALQ 158 F GY+E + R + +PL++ Y+L A++ Sbjct: 615 FYGYKEASAEFSKARKKTTYDPLNKKEYLLHKLNAIR 651 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 955,611,834 Number of extensions: 37132201 Number of successful extensions: 77934 Number of sequences better than 1.0e-05: 2795 Number of HSP's gapped: 76890 Number of HSP's successfully gapped: 2795 Length of query: 159 Length of database: 3,060,696,203 Length adjustment: 120 Effective length of query: 39 Effective length of database: 1,930,174,403 Effective search space: 75276801717 Effective search space used: 75276801717 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)