BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0005.p (388 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K812.1 B3K812_9BACI Glycerate kinase EC=2.7.1.31 456 e-126 C1PC21.1 C1PC21_BACCO Glycerate kinase EC=2.7.1.31 451 e-125 Q97FA6.1 Q97FA6_CLOAB Uncharacterized conserved protein, YHAD fa... 449 e-124 Q74J59.1 Q74J59_LACJO Glycerate kinase 443 e-122 Q897N1.1 Q897N1_CLOTE Glycerate kinase EC=2.7.1.31 432 e-119 >B3K812.1 B3K812_9BACI Glycerate kinase EC=2.7.1.31 Length = 382 Score = 456 bits (1172), Expect = e-126, Method: Compositional matrix adjust. Identities = 226/375 (60%), Positives = 277/375 (73%) Query: 10 KEKTFVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGG 69 ++ VLAPDSFKESMTAKEVC AME+G++K P IHVPMADGGEGT+QSLVDA+GG Sbjct: 3 QDYVIVLAPDSFKESMTAKEVCEAMERGIKKGNPNVTCIHVPMADGGEGTMQSLVDATGG 62 Query: 70 EIHYKEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELI 129 +H V GPLG +V A YGI G G T +EMASASGIHLVP R+PL+TTTYGTGELI Sbjct: 63 SVHTVTVTGPLGDQVEASYGISGDGSTGVLEMASASGIHLVPIAQRDPLVTTTYGTGELI 122 Query: 130 RECLDXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISA 189 + CLD SATNDGGAG+ +ALG + LD G EL GGG L RLA +D+S Sbjct: 123 KACLDHGVSRLLIGIGGSATNDGGAGVVQALGGKLLDAEGKELAFGGGELGRLARLDLSG 182 Query: 190 LDERLQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLH 249 D RLQ V++ VACDVTNPLCGE GAS+VFGPQKGATPEM+++LD NL +YA V+KQQL Sbjct: 183 FDPRLQDVEVEVACDVTNPLCGETGASQVFGPQKGATPEMIEQLDRNLRNYAGVMKQQLG 242 Query: 250 KDVRDLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQ 309 KD+ D P F L+KGI++V++YTGL+EK+ AD+V+TGEGG+DFQ Sbjct: 243 KDIIDYPGAGAAGGLGGGLMAFLNGTLKKGIDMVVDYTGLEEKVRQADMVWTGEGGMDFQ 302 Query: 310 TKFGKTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEG 369 T++GKTP GVAR AK G+ V+A+AG +G+GID LY++G AIFGI+PGA+ L + LA G Sbjct: 303 TQYGKTPLGVARVAKKHGKPVVALAGRVGDGIDVLYDQGMDAIFGIMPGAATLSEALANG 362 Query: 370 PQNVERTCENIARIL 384 QNVERT ENI R++ Sbjct: 363 VQNVERTSENIVRLM 377 >C1PC21.1 C1PC21_BACCO Glycerate kinase EC=2.7.1.31 Length = 356 Score = 451 bits (1159), Expect = e-125, Method: Compositional matrix adjust. Identities = 228/355 (64%), Positives = 267/355 (75%) Query: 34 MEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHYKEVNGPLGQKVTAKYGILGG 93 ME G++K P A I VPMADGGEGTVQSLVDA+GG I+ + V GPLG VTA YGILG Sbjct: 1 MEAGIKKAIPDAVCIQVPMADGGEGTVQSLVDATGGTIYQQTVTGPLGHPVTACYGILGD 60 Query: 94 GQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECLDXXXXXXXXXXXXSATNDGG 153 G+TA IEMASASGIHLV KET+NPL+TTTYGTGELI+ CLD SATNDGG Sbjct: 61 GETAVIEMASASGIHLVTKETKNPLVTTTYGTGELIKACLDKKVKQILIGIGGSATNDGG 120 Query: 154 AGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDERLQHVQMIVACDVTNPLCGEQ 213 AGMA+ALG +FLDE G LP GGG+L LA ID++ LD RL+ VQ+ VA DVTNPLCGE Sbjct: 121 AGMAQALGVKFLDERGGMLPFGGGALGDLAEIDLAGLDPRLKEVQIFVASDVTNPLCGEN 180 Query: 214 GASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVRDLPXXXXXXXXXXXXXIFTQ 273 GAS VFGPQKGAT EMV LD NL+HYAA++K+QL KDV ++P +FT Sbjct: 181 GASHVFGPQKGATKEMVALLDTNLSHYAAIIKEQLGKDVAEVPGAGAAGGLGAGLMVFTD 240 Query: 274 AVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFGKTPYGVARAAKAGGQKVIAV 333 AVL+ GIE+V+EYTGL++K+ AD+V TGEG IDFQT+FGKTPYGVARAAK G+ VIAV Sbjct: 241 AVLKSGIELVVEYTGLEQKVKDADIVLTGEGSIDFQTQFGKTPYGVARAAKKHGKPVIAV 300 Query: 334 AGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNVERTCENIARILKLSE 388 AGY+GE ID LYE+G AIFGI+P LE L G N+ERTCENIAR+L L++ Sbjct: 301 AGYVGEQIDVLYEKGIDAIFGILPRPMALEDALQTGAANIERTCENIARLLVLAK 355 >Q97FA6.1 Q97FA6_CLOAB Uncharacterized conserved protein, YHAD family Length = 380 Score = 449 bits (1154), Expect = e-124, Method: Compositional matrix adjust. Identities = 221/378 (58%), Positives = 276/378 (73%) Query: 10 KEKTFVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGG 69 K T VLAPDSFKESMTAKEVC+AMEKG++KV + IHVPMADGGEGT+QSLVDA+ G Sbjct: 2 KSFTIVLAPDSFKESMTAKEVCLAMEKGIKKVNDRINCIHVPMADGGEGTMQSLVDATNG 61 Query: 70 EIHYKEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELI 129 +++ V GPL KV A+YGILG G+ IEMASASGIHLVPKE RNPLITTTYGTGEL+ Sbjct: 62 KVYSLNVMGPLLDKVEAQYGILGNGEIGVIEMASASGIHLVPKEKRNPLITTTYGTGELV 121 Query: 130 RECLDXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISA 189 + CLD S+TNDGG G +ALGA+FLDE G ++ GGGSL +L ID+S Sbjct: 122 KACLDRGVKKILIGIGGSSTNDGGVGAIKALGAKFLDEEGKDIGFGGGSLSKLRKIDLSE 181 Query: 190 LDERLQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLH 249 LDERL V++ VACDV NPLCG+ GAS +FGPQKGAT EMV+ LD NL HYA ++K+Q Sbjct: 182 LDERLSKVEIEVACDVNNPLCGKNGASNIFGPQKGATKEMVETLDENLKHYAKIIKEQCK 241 Query: 250 KDVRDLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQ 309 KDV D+P F L++GI +VI+Y+GL+EK+ D+VFTGEG ID+Q Sbjct: 242 KDVIDMPGAGAAGGLGAGLVAFLDGKLERGIGLVIKYSGLEEKVKDCDIVFTGEGSIDYQ 301 Query: 310 TKFGKTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEG 369 TKFGKTP GVA+ AK + VIA+AG +G+ I+ LYEEG +IFGI+ G LE++L +G Sbjct: 302 TKFGKTPIGVAKLAKKYDKPVIALAGRVGDNIEDLYEEGIDSIFGIIRGVKTLEEVLKDG 361 Query: 370 PQNVERTCENIARILKLS 387 +N+E+T ENI R++ L+ Sbjct: 362 KENIEKTSENIMRLINLN 379 >Q74J59.1 Q74J59_LACJO Glycerate kinase Length = 376 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 223/373 (59%), Positives = 274/373 (73%) Query: 14 FVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHY 73 +V+APDSFKESMTAKEVC AMEKG++K AA I VPMADGGEGTV SLVDA+ G+ Sbjct: 4 YVVAPDSFKESMTAKEVCDAMEKGIKKADSAAEVIKVPMADGGEGTVNSLVDATHGQRVI 63 Query: 74 KEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECL 133 EV GPLG K++A YGILG G TA IEMA ASG+ +V K+ RNP+ITTT+GTGELIR+ L Sbjct: 64 VEVTGPLGNKISAYYGILGNGTTAVIEMAKASGLEIVEKKKRNPMITTTFGTGELIRDAL 123 Query: 134 DXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDER 193 D S+TNDGG+GMA+ALGA+ LD++ ++P GGG+LD+L IDIS LD R Sbjct: 124 DHNVKEIIIGLGGSSTNDGGSGMAQALGAKLLDQNNHQIPFGGGNLDKLDKIDISNLDSR 183 Query: 194 LQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVR 253 LQ V++I+A DVTNPL G++GASRVFG QKGATPEMV+KL+ NL HYA +VK+ L+KDV Sbjct: 184 LQDVKIILASDVTNPLIGKEGASRVFGLQKGATPEMVEKLENNLQHYAKIVKRDLNKDVA 243 Query: 254 DLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFG 313 + FT +++G+++ IE T L+EK+ D VFTGEGG DFQTKFG Sbjct: 244 SVSGAGAAGGLGAGLMAFTTCEMRQGVDLAIEVTKLEEKIRDTDYVFTGEGGTDFQTKFG 303 Query: 314 KTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNV 373 KTPYGVA+ K + VI++AGY+GEGID+LY EGFTAIFGI+PGA L L GP NV Sbjct: 304 KTPYGVAKLGKKYHKPVISLAGYLGEGIDSLYSEGFTAIFGIIPGACDLSTALKNGPSNV 363 Query: 374 ERTCENIARILKL 386 RT ENI R+LK Sbjct: 364 ARTTENIVRLLKF 376 >Q897N1.1 Q897N1_CLOTE Glycerate kinase EC=2.7.1.31 Length = 378 Score = 432 bits (1112), Expect = e-119, Method: Compositional matrix adjust. Identities = 214/372 (57%), Positives = 268/372 (72%) Query: 14 FVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHY 73 FVLAPDSFKESMTAKEV +ME GL+ ++P A YI VPMADGGEGT+Q+L+DA+ GEI Sbjct: 3 FVLAPDSFKESMTAKEVATSMENGLKNIFPKADYIKVPMADGGEGTLQALIDATNGEIFK 62 Query: 74 KEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECL 133 EV GPLG+ +TA++GILG G TA IEMASASG++ + E RNPLITTT+GTGELI+ L Sbjct: 63 VEVLGPLGEPITAEFGILGNGTTAVIEMASASGLYHIEPENRNPLITTTFGTGELIKHAL 122 Query: 134 DXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDER 193 D SATNDGGAGM +ALG + L +SG ++P GGG+L++L SID+S LD R Sbjct: 123 DKNVHQIFIGIGGSATNDGGAGMIQALGGKLLTKSGEDIPFGGGALNKLHSIDLSNLDPR 182 Query: 194 LQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVR 253 L V + VACDV NPL G+ GAS +FGPQKGATPE+V+ LD NL HYA V+K+ L D+ Sbjct: 183 LSEVSIDVACDVNNPLTGKNGASYIFGPQKGATPEIVELLDENLKHYAKVIKKYLQMDIE 242 Query: 254 DLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFG 313 +P F A L+KG+++VIEYT L+EKL AD VFTGEG ID+QTKFG Sbjct: 243 SIPGAGAAGGLGGGLMAFLSANLKKGVDLVIEYTHLEEKLKNADFVFTGEGAIDYQTKFG 302 Query: 314 KTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNV 373 KTPYGVA AK VI ++G IG+G + LYE G T+IFGI+PGA +++ L G N+ Sbjct: 303 KTPYGVASIAKKYSIPVIVLSGKIGDGCEVLYEGGITSIFGILPGAVDIKEALKSGAVNI 362 Query: 374 ERTCENIARILK 385 ERT EN+ R++K Sbjct: 363 ERTSENVGRLIK 374 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,220,629,873 Number of extensions: 93814645 Number of successful extensions: 232931 Number of sequences better than 1.0e-05: 1081 Number of HSP's gapped: 231058 Number of HSP's successfully gapped: 1095 Length of query: 388 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 250 Effective length of database: 1,760,596,133 Effective search space: 440149033250 Effective search space used: 440149033250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 132 (55.5 bits)