BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0001.p (398 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGD3.1 B3KGD3_9BACI Major facilitator superfamily MFS_1 450 e-124 C0Z5D0.1 C0Z5D0_BREBN Multidrug resistance protein 432 e-119 B1D626.1 B1D626_9BACL Major facilitator superfamily MFS_1 422 e-116 A4ING5.1 A4ING5_GEOTN Multidrug-efflux transporter 360 1e-97 Q5WGK5.1 Q5WGK5_BACSK Major facilitator (MFS) superfamily multid... 357 1e-96 >B3KGD3.1 B3KGD3_9BACI Major facilitator superfamily MFS_1 Length = 401 Score = 450 bits (1157), Expect = e-124, Method: Compositional matrix adjust. Identities = 244/381 (64%), Positives = 286/381 (75%), Gaps = 6/381 (1%) Query: 1 MLLLMLNIFLAFTGIGLVVPIMPTYMNEXXXXXXXXXXXXAAFSLTQLILSPLAGGLSDR 60 +LLLM NIFL FTGIGLV+PIMPTYM E AAFSLTQL+ SPLAG LSD Sbjct: 11 ILLLMFNIFLVFTGIGLVIPIMPTYMTELHISGSTVGMLVAAFSLTQLLFSPLAGRLSDI 70 Query: 61 MGRKKIIVGGLVVFALSELLFGLAESSWLLFASRMLGGAGAAMIMPAVMAYVADTTSMEE 120 MGRKKII+ G++VFA+SE LFG A + LLFA+RMLGG GAA+IMPAVMAY AD TS EE Sbjct: 71 MGRKKIIISGMIVFAISEWLFGAASTPTLLFAARMLGGIGAALIMPAVMAYTADVTSNEE 130 Query: 121 RAKGMGLINAAITTGFIIGPGLGGYLAELGIRXXXXXXXXXXXXXXXMTLFVLPESRSSA 180 RA+GMG INAAITTGFIIGPG+GG++AE GIR +TL +LPES + Sbjct: 131 RARGMGFINAAITTGFIIGPGIGGFIAEFGIRVPFYAAAVAGGIAALVTLLLLPESSAKQ 190 Query: 181 ELGAAKSTQENKDSKDSLITQLVRSYREPYFFGLVIVFVLSFGLANYETVFGLFVDHKFG 240 E A + K ++SL QLVRS+REPYF L+IVFVLSFGLANYETVFGLFVDHKFG Sbjct: 191 E---APTVLGRK--QESLAVQLVRSFREPYFLSLIIVFVLSFGLANYETVFGLFVDHKFG 245 Query: 241 FTPKDIAFVITFGSISGAVIQVTAFSWILNRFGENKVIXXXXXXXXXXXXXXXFVHGYWA 300 FTPKDIA +ITFGSI+GAV+Q T F WILNRFGE +VI FVH YW Sbjct: 246 FTPKDIAMIITFGSIAGAVVQATIFGWILNRFGEKRVITLSLLTAGICILLTLFVHKYWL 305 Query: 301 IVTVTFIVFLAMDILRPAVGTQLSKMASESQQGFVMGMNSAYTSLGNIAGPIVAGVLFDL 360 I VTF+VFL++DILRPA+GTQ+SK+A + QQG+V G+NSAYTSLGNIAGP+VAG LFDL Sbjct: 306 IFLVTFLVFLSIDILRPAIGTQMSKLAKD-QQGYVAGLNSAYTSLGNIAGPLVAGYLFDL 364 Query: 361 DINLPYTVAALVLMLCFIMSL 381 DIN PY A++VL+LCF +++ Sbjct: 365 DINFPYISASIVLILCFFLAM 385 >C0Z5D0.1 C0Z5D0_BREBN Multidrug resistance protein Length = 409 Score = 432 bits (1111), Expect = e-119, Method: Compositional matrix adjust. Identities = 236/398 (59%), Positives = 291/398 (73%), Gaps = 4/398 (1%) Query: 1 MLLLMLNIFLAFTGIGLVVPIMPTYMNEXXXXXXXXXXXXAAFSLTQLILSPLAGGLSDR 60 +L+LM NIFL FTGIGLV+PIMP YM AAFSLTQL+ SP+AG +D Sbjct: 11 LLILMFNIFLVFTGIGLVIPIMPKYMESLGITGGTIGLLVAAFSLTQLLFSPIAGRWADS 70 Query: 61 MGRKKIIVGGLVVFALSELLFGLAESSWLLFASRMLGGAGAAMIMPAVMAYVADTTSMEE 120 GRKKIIV GLVVFA+SE++FG+A S LLF SR+LGG AA+IMPAVMAY AD T+ EE Sbjct: 71 FGRKKIIVIGLVVFAISEVMFGIANSPVLLFVSRLLGGISAALIMPAVMAYSADITTNEE 130 Query: 121 RAKGMGLINAAITTGFIIGPGLGGYLAELGIRXXXXXXXXXXXXXXXMTLFVLPESRSSA 180 RA GMG I AAITTGFIIGPG+GGY+AE GIR +TL +L ES+ + Sbjct: 131 RAAGMGYITAAITTGFIIGPGIGGYIAEFGIRVPFYSAGIAGLIAACITLLILKESKPTE 190 Query: 181 ELGAAKSTQENKDSKDSLITQLVRSYREPYFFGLVIVFVLSFGLANYETVFGLFVDHKFG 240 E + + ++ + SL++QL+ SYREPYFF L+IVFV+SFGLAN+ETVF LFVDHKFG Sbjct: 191 E--SLADSSSSEKQRSSLLSQLMYSYREPYFFSLIIVFVMSFGLANFETVFSLFVDHKFG 248 Query: 241 FTPKDIAFVITFGSISGAVIQVTAFSWILNRFGENKVIXXXXXXXXXXXXXXXFVHGYWA 300 F PKDIAF+ITFGSI+GAV+Q+TAFSWI+NRFGE +VI FVHG+W Sbjct: 249 FEPKDIAFIITFGSIAGAVVQLTAFSWIMNRFGEKRVISICLLFAGLFIILTLFVHGFWM 308 Query: 301 IVTVTFIVFLAMDILRPAVGTQLSKMASESQQGFVMGMNSAYTSLGNIAGPIVAGVLFDL 360 I VTFIVFLA+DILRPA+ TQ+S +A + QQG+V G+NSA+TSLGNIAGPIVAG LFD+ Sbjct: 309 IFAVTFIVFLAIDILRPAISTQMSMLAKD-QQGYVAGLNSAFTSLGNIAGPIVAGFLFDI 367 Query: 361 DINLPYTVAALVLMLCFIMSLGSRKRMNRPDLQAKQAD 398 DIN PY +A L+L++CF++SLG + R N D + + Sbjct: 368 DINYPYGLACLILLICFVLSLGVKNR-NLKDAKTASSQ 404 >B1D626.1 B1D626_9BACL Major facilitator superfamily MFS_1 Length = 400 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 227/375 (60%), Positives = 271/375 (72%), Gaps = 3/375 (0%) Query: 12 FTGIGLVVPIMPTYMNEXXXXXXXXXXXXAAFSLTQLILSPLAGGLSDRMGRKKIIVGGL 71 FTGIGLV+PIMP +M AAFSLTQL+ SPLAG LSD+ GRK++IV G+ Sbjct: 22 FTGIGLVIPIMPKFMETLGITGGIVGLLVAAFSLTQLLFSPLAGRLSDKFGRKRMIVIGM 81 Query: 72 VVFALSELLFGLAESSWLLFASRMLGGAGAAMIMPAVMAYVADTTSMEERAKGMGLINAA 131 +VFA+SE +FG AESS LLF SR+LGG AAMIMPAVMAY AD T+ EERA GMG INAA Sbjct: 82 LVFAVSEAMFGFAESSVLLFVSRLLGGVSAAMIMPAVMAYAADITTKEERASGMGFINAA 141 Query: 132 ITTGFIIGPGLGGYLAELGIRXXXXXXXXXXXXXXXMTLFVLPESRSSAELGAAKSTQEN 191 ITTGFIIGPG+GGY+AE GIR + L +LPES+ E A T Sbjct: 142 ITTGFIIGPGIGGYIAEFGIRAPFYAAGAAGVLAAVINLIILPESKPVQE--EATETSAA 199 Query: 192 KDSKDSLITQLVRSYREPYFFGLVIVFVLSFGLANYETVFGLFVDHKFGFTPKDIAFVIT 251 + ITQL SYREPYF L+IVFV+SFGLAN+ETVFGLFVDHKFG+ PKDIAF+IT Sbjct: 200 GKEQRGFITQLRYSYREPYFLALIIVFVMSFGLANFETVFGLFVDHKFGYEPKDIAFIIT 259 Query: 252 FGSISGAVIQVTAFSWILNRFGENKVIXXXXXXXXXXXXXXXFVHGYWAIVTVTFIVFLA 311 FGSI+GAV+QVT FSWIL FGE +VI FVH +W + VTFI+FLA Sbjct: 260 FGSIAGAVVQVTIFSWILRTFGERRVISICLLFAGLFIVLTLFVHKFWMVFAVTFIIFLA 319 Query: 312 MDILRPAVGTQLSKMASESQQGFVMGMNSAYTSLGNIAGPIVAGVLFDLDINLPYTVAAL 371 +DILRPA+ TQ+S M ++ QQG+V G+NSA+TSLGNIAGPI+AG LFD+DIN PYT+A + Sbjct: 320 IDILRPAISTQMS-MVAKDQQGYVAGLNSAFTSLGNIAGPIIAGFLFDMDINYPYTLACV 378 Query: 372 VLMLCFIMSLGSRKR 386 VL+LCF ++L RKR Sbjct: 379 VLLLCFALTLSDRKR 393 >A4ING5.1 A4ING5_GEOTN Multidrug-efflux transporter Length = 404 Score = 360 bits (925), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 194/385 (50%), Positives = 259/385 (67%), Gaps = 7/385 (1%) Query: 1 MLLLMLNIFLAFTGIGLVVPIMPTYMNEXXXXXXXXXXXXAAFSLTQLILSPLAGGLSDR 60 ML+LM N+ L F GIGL++PIMPT++ + A FSLTQ + SP+AG ++D Sbjct: 11 MLILMFNLLLIFIGIGLIIPIMPTFIEQLGISGGTVGLLVAVFSLTQFLFSPVAGRMADA 70 Query: 61 MGRKKIIVGGLVVFALSELLFGLAESSWLLFASRMLGGAGAAMIMPAVMAYVADTTSMEE 120 +GRKK+IV G+++FA SE FG A+ + +F SR++GG AA+ MPAVMAY AD T+ EE Sbjct: 71 VGRKKLIVSGMLLFAFSEWQFGYADHVFEMFFSRVIGGISAALTMPAVMAYAADITTNEE 130 Query: 121 RAKGMGLINAAITTGFIIGPGLGGYLAELGIRXXXXXXXXXXXXXXXMTLFVLPESRSSA 180 RA G+G INAAIT GF+IGPG+GGY+AE GIR +T LPES SSA Sbjct: 131 RAAGIGYINAAITMGFMIGPGIGGYMAEWGIRVPFNCAAAVGVMAAVITALFLPESHSSA 190 Query: 181 ELGAAKSTQENKDSKDSLITQLVRSYREPYFFGLVIVFVLSFGLANYETVFGLFVDHKFG 240 G + KD + QL + +R+PYFF L+I+F LSFGLAN+ET+F LFVDH+FG Sbjct: 191 STGTVQ-----KDHVPFFV-QLAQVHRQPYFFSLLIIFALSFGLANFETIFSLFVDHRFG 244 Query: 241 FTPKDIAFVITFGSISGAVIQVTAFSWILNRFGENKVIXXXXXXXXXXXXXXXFVHGYWA 300 F+PK+IA +IT GSI G ++Q+T F W+L FGE +V+ + Sbjct: 245 FSPKEIALIITIGSIGGPLVQLTTFGWLLGHFGEQRVVSACLLLSALFIVLTLLASTWTM 304 Query: 301 IVTVTFIVFLAMDILRPAVGTQLSKMASESQQGFVMGMNSAYTSLGNIAGPIVAGVLFDL 360 ++ T +VFLA+DILRPAV TQ+SK+ E+QQG++ G+NSA+TSLGNI GP VAGVLFD+ Sbjct: 305 LLVATVLVFLAIDILRPAVSTQMSKI-DETQQGYIAGLNSAFTSLGNIIGPAVAGVLFDV 363 Query: 361 DINLPYTVAALVLMLCFIMSLGSRK 385 DI PY +A++VL LCF++SL K Sbjct: 364 DIRFPYILASVVLFLCFVLSLRQEK 388 >Q5WGK5.1 Q5WGK5_BACSK Major facilitator (MFS) superfamily multidrug resistance protein Length = 403 Score = 357 bits (916), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 188/390 (48%), Positives = 256/390 (65%), Gaps = 5/390 (1%) Query: 1 MLLLMLNIFLAFTGIGLVVPIMPTYMNEXXXXXXXXXXXXAAFSLTQLILSPLAGGLSDR 60 +LLLM NIF+ F G+GLV+PIMPTYMN A FSLTQ +++P AG SDR Sbjct: 12 ILLLMFNIFIGFVGVGLVIPIMPTYMNVLGISGTIVGLLVAVFSLTQFLVAPYAGSWSDR 71 Query: 61 MGRKKIIVGGLVVFALSELLFGLAESSWLLFASRMLGGAGAAMIMPAVMAYVADTTSMEE 120 GRK IIV GL++FA+SELLFGLA ++ LLF SR+LGG A IMPAVMAYV D T+ E+ Sbjct: 72 YGRKWIIVAGLLLFAVSELLFGLATNAVLLFISRLLGGVSVAFIMPAVMAYVVDITTEED 131 Query: 121 RAKGMGLINAAITTGFIIGPGLGGYLAELGIRXXXXXXXXXXXXXXXMTLFVLPESRSSA 180 R GMG INAAI+TGFIIGP +GG+L E G+R +++ +LPES Sbjct: 132 RGMGMGWINAAISTGFIIGPAIGGFLVEYGMRVPFFAAAGAAALSAVVSMSILPESLDKN 191 Query: 181 ELGAAKSTQENKDSKDSLITQLVRSYREPYFFGLVIVFVLSFGLANYETVFGLFVDHKFG 240 +L A ++ + K+S+ + QL +SYR PYF GL+I+ + S GL+ +ETV GLFVDHK+ Sbjct: 192 KLPAPEARMDRKESQ---LAQLAKSYRTPYFTGLLIILITSLGLSQFETVLGLFVDHKYS 248 Query: 241 FTPKDIAFVITFGSISGAVIQVTAFSWILNRFGENKVIXXXXXXXXXXXXXXXFVHGYWA 300 +TP +IA++I G+I GAV+Q+T F ++N GE K+ F YW Sbjct: 249 YTPNEIAWLIMIGAIVGAVMQLTLFGRLINWIGEKKLTVYCLLVMAVFMVATIFSAQYWM 308 Query: 301 IVTVTFIVFLAMDILRPAVGTQLSKMASESQQGFVMGMNSAYTSLGNIAGPIVAGVLFDL 360 +V +VFLA+D +RPA+ T S++A ++QG V G+N++YTSLGNI GPI+AG LFD+ Sbjct: 309 MVVSVTLVFLAVDFVRPAISTYFSRIAG-NEQGLVAGLNASYTSLGNIGGPILAGALFDV 367 Query: 361 DINLPYTVAALVLMLCFIMSL-GSRKRMNR 389 IN P+ V +L+ + +I+SL RK+ R Sbjct: 368 QINSPFLVGSLIALGGWILSLRWMRKQTER 397 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.141 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,933,557,162 Number of extensions: 69845390 Number of successful extensions: 342277 Number of sequences better than 1.0e-05: 7487 Number of HSP's gapped: 339466 Number of HSP's successfully gapped: 7522 Length of query: 398 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 260 Effective length of database: 1,760,596,133 Effective search space: 457754994580 Effective search space used: 457754994580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)