BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0238.0004.p (608 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9AJR6.2 Q9AJR6_BACGL Alpha-D-mannosidase 380 e-103 A5UT79.1 A5UT79_ROSS1 Alpha-mannosidase EC=3.2.1.24 374 e-101 A7NMZ2.1 A7NMZ2_ROSCS Alpha-mannosidase EC=3.2.1.24 370 e-100 Q5KYP9.1 Q5KYP9_GEOKA Alpha-D-mannosidase EC=3.2.1.24 369 e-100 A9KS60.1 A9KS60_CLOPH Glycoside hydrolase family 38 367 1e-99 >Q9AJR6.2 Q9AJR6_BACGL Alpha-D-mannosidase Length = 1047 Score = 380 bits (976), Expect = e-103, Method: Compositional matrix adjust. Identities = 236/613 (38%), Positives = 323/613 (52%), Gaps = 70/613 (11%) Query: 10 GKQSELKNACNESGGGQPQ-LPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELW 68 G+ + + +E+ G + + W G+LYLE HRGTYT+ NKR NRK E++YREAE Sbjct: 489 GRAGDFFDKLHETVAGTDRYVHTWDGELYLEYHRGTYTSQAYNKRMNRKLELMYREAEWL 548 Query: 69 QTLAGSSIAPELQRSIAGQLHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGE 128 +L + + L++GW +L NQFHDIIPGS+I Y+ + EY E LG+ Sbjct: 549 HSLGAVAGGGDWSGYPKEALYEGWTTILRNQFHDIIPGSSIGAVYQDARAEYAEAERLGK 608 Query: 129 SSLQPGLEALTAQVAAAGEGIPYVLFNSLGWERDAVV--PVHLPEGRTWAAYDEAGSPLH 186 ++Q E L Q EG ++N ++R +V PV G EAG PL Sbjct: 609 EAIQAAAERLAGQ----AEGAVVSVYNGAAFDRTELVEIPVQAGIGSQGGWLGEAGRPLR 664 Query: 187 --------LDLAADLSARDXXXXXXXXXXXXXXXXXSIERATEQATGQVTAYIHVPSIPG 238 L LA D+ A + + GQ T S+ Sbjct: 665 AEFDGSRWLVLAEDVPATGAATLRF-----------------DPSAGQQT------SVKA 701 Query: 239 FGYKTVWLRQVDEAVTGSMPERRELNDSWETPHYRLAFNERGEITSLFDKSAGREVVQPG 298 F + LR TPHY LAF+E G + L+D+ REV+ G Sbjct: 702 FEWADGVLR---------------------TPHYELAFDEAGRLVRLYDRGLSREVLPRG 740 Query: 299 GRANRFHFFHDRPTLWDAWDIDSRYEAQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSE 358 RAN F D+P +++AWDID Y+ + +L + ++ G ++ VLR W G S Sbjct: 741 ARANELQVFEDKPKMFEAWDIDLFYQEKRREVTDLRKLELVECGALRAVLRLEWAYGASV 800 Query: 359 ITQDLILYAHDRRIDFKTHVDWNEAHKLLKVGFPVDVVADKATYEIPFGALERPTHRNTS 418 I Q L++YA DRRIDF T VDW E +LLKV FPV++ A +ATY+I FG ++RPTH NTS Sbjct: 801 IEQRLVVYADDRRIDFVTRVDWREHQQLLKVAFPVEIRATEATYDIQFGNVKRPTHWNTS 860 Query: 419 WEQAQYEVCGHRFADVSEHGYGVSLLNDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAH 478 W+ A++E GH++ D+SE GYGVSLLNDCKYG+DI+ + IRLSL+++ PD ADQG H Sbjct: 861 WDHARFETVGHQWVDLSERGYGVSLLNDCKYGHDIKDNVIRLSLIKSAIDPDPNADQGEH 920 Query: 479 DFTYALYPHTGDWRSAHTLRKAAELNHEVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDT 538 FTY+LYPH GDW S T A LN+ + A+ G+ S +R DG H+ D Sbjct: 921 LFTYSLYPHAGDWASGGTATAAWALNNPLLAVPGASAFAGK-----SLLRADGAHIAFDA 975 Query: 539 VKPAEDGRGSVLRLYESSGGRETVKLAWNQPFSEIYISNALEAELEPV-IHHEGEFELNF 597 +K AED V+RL+E +GGR L P EI + P+ H+G+ L F Sbjct: 976 IKKAEDEDALVVRLHEYAGGRGLTTLT---PGCEIASWQECDLLERPIGDRHDGDKPLRF 1032 Query: 598 --APFEIKTIYIR 608 AP+EIKT IR Sbjct: 1033 ELAPYEIKTFLIR 1045 >A5UT79.1 A5UT79_ROSS1 Alpha-mannosidase EC=3.2.1.24 Length = 1057 Score = 374 bits (961), Expect = e-101, Method: Compositional matrix adjust. Identities = 217/586 (37%), Positives = 312/586 (53%), Gaps = 53/586 (9%) Query: 25 GQPQLPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSI 84 P+LP W G+LYLE HRGTYT+ R K+NNR+AE+L REAE A + A Q Sbjct: 522 NNPRLPVWVGELYLEYHRGTYTSQARTKQNNRQAELLLREAEWLNAWAVTQGAINRQ--- 578 Query: 85 AGQLHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAA 144 L W+ VLLNQFHDI+PGS++ Y SE+++ EV E+ + LT + Sbjct: 579 -ADLDAAWRTVLLNQFHDILPGSSVPLVYVESERQFAEVRETVETVRHAAITDLT-ESDG 636 Query: 145 AGEGIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXX 204 G +FNSL W+R V + L D+ P Sbjct: 637 NGATPRLAVFNSLAWDRTECVAIPL---------DDRPRP-------------------- 667 Query: 205 XXXXXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELN 264 + + E G + S+P FGY + D+ + P R + Sbjct: 668 -------AVAGVTQTVEHLDGTRALLVEA-SVPAFGYTAI-----DKVSRPTPPARFTVR 714 Query: 265 DSW-ETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRY 323 + + +R+ F+ GEI SL+D RE++ G N+ + DRP WDAWDID Y Sbjct: 715 REYLASDEFRIEFDTHGEIGSLYDARYEREIIAEGATGNQLVLYEDRPMFWDAWDIDPFY 774 Query: 324 EAQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEA 383 +P ++ E V+ G ++ + + G+S I Q + L+ + RRIDF T VDW E Sbjct: 775 LEKPYPIHDISEWRVVEEGPLRATVEIVRRFGKSTIRQRISLWRNSRRIDFDTEVDWQER 834 Query: 384 HKLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSL 443 LL+V FP+ + A +AT EI FGA+ERPTHRNTSW+ A++EVC HR+ D+SE GYGV+L Sbjct: 835 QNLLRVLFPLRLNATRATCEIQFGAVERPTHRNTSWDWARFEVCAHRWVDLSEGGYGVAL 894 Query: 444 LNDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAEL 503 +N+ KYG+ + +T+ +SLL++ PD AD+G H F Y+L PH GDWR A R+A EL Sbjct: 895 MNNGKYGHSLLHNTLGISLLKSAVIPDPQADRGVHRFMYSLLPHAGDWREAQVTRRAYEL 954 Query: 504 NHEVPAIAADGKTTGQLPSTG-SFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETV 562 N + + G++P+ G SF+R + HVV++T+K A+DG G ++R+YE+ R V Sbjct: 955 NAPLRPVT----VMGEVPAEGRSFLRVESDHVVVETIKTADDGDGLIVRMYEAHNQRGQV 1010 Query: 563 KLAWNQPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKTIYIR 608 +L + +P + LE E PV + N PFEIKTI +R Sbjct: 1011 RLDFGRPVESAVEVDLLERETGPVTVDGQTVQCNVRPFEIKTIRVR 1056 >A7NMZ2.1 A7NMZ2_ROSCS Alpha-mannosidase EC=3.2.1.24 Length = 1061 Score = 370 bits (951), Expect = e-100, Method: Compositional matrix adjust. Identities = 218/585 (37%), Positives = 309/585 (52%), Gaps = 51/585 (8%) Query: 25 GQPQLPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSI 84 P+LP W G+LYLE HRGTYT+ R K+NNR+AE+L REAE A + A Q Sbjct: 522 NNPRLPVWVGELYLEYHRGTYTSQARTKQNNRQAELLLREAEWLNAWAVTHGAINRQ--- 578 Query: 85 AGQLHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAA 144 L W+ VLLNQFHDI+PGS++ Y SE+++ EV E+ + LT + Sbjct: 579 -ADLDAAWRTVLLNQFHDILPGSSVPLVYVESEQQFAEVRDTVETVRHAAITDLTGS-HS 636 Query: 145 AGEGIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXX 204 G +FN L W+R V + L + R PL Sbjct: 637 NGAAPHLAVFNGLAWDRTECVAIPLEDLR---------QPL------------------- 668 Query: 205 XXXXXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELN 264 + EQ G T + ++P FGY + + + R Sbjct: 669 --------IEGPTQVVEQIDGTRTLLVEA-TVPAFGYAALRAARHPSPPAQFITRR---- 715 Query: 265 DSWETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYE 324 D + +R+ F+ GEI SL+D RE++ G N+ + DRP WDAWDID Y Sbjct: 716 DYLASDEFRIEFDANGEIGSLYDARYEREIIAEGATGNQLVLYEDRPMFWDAWDIDPFYL 775 Query: 325 AQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAH 384 +P + E V+ G ++ + + G+S I Q + L+ ++RRIDF+T VDW E Sbjct: 776 EKPYPIHNISEWRVIEEGPLRATVEIARRFGRSTIRQRISLWHNNRRIDFETEVDWQERQ 835 Query: 385 KLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLL 444 LL+V FP+ + A +AT EI FGA+ERPTHRNTSW+ A++EVC HR+ D+SE GYGV+LL Sbjct: 836 NLLRVLFPLRLNAARATCEIQFGAVERPTHRNTSWDWARFEVCAHRWIDLSEGGYGVALL 895 Query: 445 NDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELN 504 N+ KYG+ + +T+ +SLL++ PD AD+G H F Y+L PH GDWR A R+A ELN Sbjct: 896 NNGKYGHSLLHNTLGISLLKSAVIPDPQADRGLHRFIYSLLPHAGDWREAQVTRRAYELN 955 Query: 505 HEVPAIAADGKTTGQLPSTG-SFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETVK 563 + + G++P+ G SF+ D HV+++T+K A+DG G ++R+YE+ R V+ Sbjct: 956 APLRPVT----VAGEIPAEGRSFLHVDSDHVIVETIKTADDGDGLIVRMYEAHNQRGQVR 1011 Query: 564 LAWNQPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKTIYIR 608 L + QP + LE E PV N PFEIKTI +R Sbjct: 1012 LEFGQPVESAVEVDLLERETGPVTVDGRTVRCNVRPFEIKTIRVR 1056 >Q5KYP9.1 Q5KYP9_GEOKA Alpha-D-mannosidase EC=3.2.1.24 Length = 1044 Score = 369 bits (948), Expect = e-100, Method: Compositional matrix adjust. Identities = 223/601 (37%), Positives = 322/601 (53%), Gaps = 49/601 (8%) Query: 10 GKQSELKNACNESGGGQPQ-LPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELW 68 G+ E NE Q + W G+LYLE HRGTYT+ NKR NRK E+LYRE E W Sbjct: 487 GRADEFFERLNEKIRNTDQYVHVWDGELYLEYHRGTYTSQAYNKRMNRKMELLYRETE-W 545 Query: 69 QTLAGSSIAPELQRSIAGQLHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGE 128 + +++ + R +L++GWK++L NQFHDIIPGS+I E YE S +EY + E Sbjct: 546 MGIVSATLNSDWSRYPFERLNEGWKIILRNQFHDIIPGSSIREVYEDSREEYEK----AE 601 Query: 129 SSLQPGLEALTAQVAAAGEGIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLD 188 + L+ + +G ++FNS W R +V + A + Sbjct: 602 KYALSAWKNLSESIIVEKKGDTVIVFNSSPWVRTDLVRI------------PATTKTKKG 649 Query: 189 LAADLSARDXXXXXXXXXXXXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQ 248 + D+S R+ ER + I V +P G+ T+ Sbjct: 650 IWKDVSGRELKA----------------ERVGDNWV------ILVEDVPPLGFSTIRFYD 687 Query: 249 VDEAVTGSMPERRELNDS-WETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFF 307 V+ T + S +P+Y++ +N++G+I S++D A REV+ G N F Sbjct: 688 VEADNTLVQTNMFHVGSSEVISPYYKIKWNDKGQIISIYDLQAKREVLDDGKCGNVLQVF 747 Query: 308 HDRPTLWDAWDIDSRYEAQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYA 367 D+P +DAWDID Y+ + +L + + V+ F WK S I QD+ILY Sbjct: 748 EDKPLRFDAWDIDIFYQEKKREVEDLQHVSIKEITSLYAVIHFEWKYMDSIIKQDMILYT 807 Query: 368 HDRRIDFKTHVDWNEAHKLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVC 427 H RRIDFKT VDW E ++LLKV FPV + + ATY+I FG ++RPTH NTSW+ A++E Sbjct: 808 HSRRIDFKTEVDWKEQNQLLKVAFPVKIRSTLATYDIQFGNVKRPTHWNTSWDYAKFETV 867 Query: 428 GHRFADVSEHGYGVSLLNDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPH 487 H++AD+SE GYGVSLLNDCKYGYDI+ + IRLSL+++ +PD DQG H FTY+L PH Sbjct: 868 AHQWADLSETGYGVSLLNDCKYGYDIKDNVIRLSLIKSATYPDYQQDQGYHIFTYSLLPH 927 Query: 488 TGDWRSAHTLRKAAELNHEVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRG 547 GDW +T+++A LN+ P A G+ + S + ++++D VK AED Sbjct: 928 QGDWVEGNTVKEAWYLNN--PLTYAVGEP---IRKKYSLFGTNTDNIMVDVVKRAEDSNR 982 Query: 548 SVLRLYESSGGRETVKLAWNQPFSEIYISNALEAEL-EPVIHHEGEFELNFAPFEIKTIY 606 VLRL+E +G R V + + P + +E E+ E I + EF++N P+EIKT Sbjct: 983 IVLRLHEFTGRRNKVYITSDLPIFAWQECDLMEREVGEGTISEKLEFDIN--PYEIKTFL 1040 Query: 607 I 607 I Sbjct: 1041 I 1041 >A9KS60.1 A9KS60_CLOPH Glycoside hydrolase family 38 Length = 1043 Score = 367 bits (943), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 203/574 (35%), Positives = 314/574 (54%), Gaps = 49/574 (8%) Query: 32 WRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSIAGQLHKG 91 W G+LY+E HRGT+T+H NK+ NRK E LYRE E W T+ + ++ L + L G Sbjct: 513 WDGELYMEYHRGTFTSHAYNKKMNRKMEFLYRETE-WLTVMAAMLSGSLHTAKQEDLTNG 571 Query: 92 WKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGEGIPY 151 WK++L NQFHDIIPGS+I E Y+ +Y++ ++ + L+ L + Y Sbjct: 572 WKILLTNQFHDIIPGSSIKEVYDDCVVDYKQAESIANHVKEEALQLLVRESKDT-----Y 626 Query: 152 VLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXXXXXXXXX 211 + N+ W R+ V + +GR DE G L L Sbjct: 627 TIRNNSSWLRNDCVEIPY-DGRKGNFVDEKGENLKAQL---------------------- 663 Query: 212 XXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELNDSWETPH 271 G + +++ ++ G+ V R ++ A + N ETP Sbjct: 664 -------------GPHSWIVYLQNVAPMGFAKV--RFIEAAREEEVLPFEYSNHCIETPF 708 Query: 272 YRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYEAQPAGEA 331 Y + N+ G+ITS++DK R+V+ PG RAN F D+P DAWDID Y+ + + Sbjct: 709 YLIQLNKEGQITSIYDKENKRQVIAPGERANVLQVFEDKPMDNDAWDIDIFYQEKMREVS 768 Query: 332 ELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAHKLLKVGF 391 +L + G ++ V F+WK S I QD+ LYA+ RRIDF+T+VD++E +LLKV F Sbjct: 769 DLEHFEIKEIGTLRAVFSFQWKYMNSVIKQDMTLYAYSRRIDFETYVDYHEKRQLLKVAF 828 Query: 392 PVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLLNDCKYGY 451 PVD+ + ATY+I +G ++RP H NTSW+QA++E H++ D+SE+ YGVSLLN+CKYG+ Sbjct: 829 PVDIRSTYATYDIQYGNVKRPNHWNTSWDQAKFETVAHKWVDLSEYNYGVSLLNNCKYGH 888 Query: 452 DIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELNHEVPAIA 511 D++ + +RL+L++ PD + DQG H FTY+L PH GD+ T ++A LN ++ AIA Sbjct: 889 DVKDNLLRLTLIKTAMHPDYLQDQGEHSFTYSLLPHKGDFVKGETEKEAFALNQKLEAIA 948 Query: 512 ADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETVKLAWNQPFS 571 + LP+ SF+ D +++ +D +K +ED V+R ++ SG + VKL + Sbjct: 949 GE----CVLPNNYSFVSIDNEYITIDAMKQSEDKEYLVVRFHDYSGSKNIVKLQTGFKVT 1004 Query: 572 EIYISNALEAELEPVIHHEGEFELNFAPFEIKTI 605 E ++ E ++ H++ + L P+EIKTI Sbjct: 1005 EWVEADLRERPMDDT-HYDSDIILEVKPYEIKTI 1037 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,911,148,098 Number of extensions: 170610432 Number of successful extensions: 353067 Number of sequences better than 1.0e-05: 326 Number of HSP's gapped: 351998 Number of HSP's successfully gapped: 599 Length of query: 608 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 466 Effective length of database: 1,722,912,073 Effective search space: 802877026018 Effective search space used: 802877026018 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)