BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0022.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZEZ2.1 C0ZEZ2_BREBN Putative uncharacterized protein 224 7e-57 Q97G02.1 Q97G02_CLOAB Possible aminoglycoside phosphotransferase... 203 1e-50 B8I0J4.1 B8I0J4_CLOCE Aminoglycoside phosphotransferase 175 4e-42 B3K882.1 B3K882_9BACI Aminoglycoside phosphotransferase 168 4e-40 B1DCR8.1 B1DCR8_9BACL Aminoglycoside phosphotransferase 167 1e-39 >C0ZEZ2.1 C0ZEZ2_BREBN Putative uncharacterized protein Length = 269 Score = 224 bits (570), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 113/268 (42%), Positives = 167/268 (62%), Gaps = 6/268 (2%) Query: 4 KRIGEGRTAEVQEYGTGKILKLYRADIPAEYVEHEYEVSKYVHKQGIQTPQPIELVTLGE 63 K+I EGRTAEV KILKLYR P E V++EYEV++ V I P+ +L+ Sbjct: 2 KKISEGRTAEVFAQDQEKILKLYRDGFPMEAVKYEYEVNRIVAFLEIPAPRAYDLIDFDH 61 Query: 64 RKGILFQQIHGSSMLRLIGEKPWRLGEYARQLASLHHDLHKLQ------GPEAFGKQKES 117 +KGI+F++I GS++LR+ + P L ++ A LH+ +HK + G + QKE Sbjct: 62 KKGIVFERIEGSTLLRMGVQFPNELDCLTKEFAELHYRIHKYELDVERIGGSSVLNQKEV 121 Query: 118 LRSSIVAAPMLTMEEKTPVLEKLEQLPEGDRLLHGDFHPDNVLIDGQAWTIDWMTGMSGN 177 L ++ A L+ E K ++E L+ LP+G+RL HGDFHP+NV+I + W IDWMTGM GN Sbjct: 122 LARNVQNASQLSSEVKHEIIEYLKNLPDGNRLCHGDFHPENVMIGERNWVIDWMTGMIGN 181 Query: 178 PAGDAARSVMMFSMGALPPGASLSTRLVTGFIRQRLTKGYIREYLRLSGLSYAEVNAWVL 237 PAGD AR++++F G LP A S + +R ++ Y+ +YL SGL +++++ W+L Sbjct: 182 PAGDVARTLLLFRFGTLPDEAPSSVKDALELMRDKINGVYLEQYLSYSGLQFSDIDEWML 241 Query: 238 PVASARLTEGIPLAEKEQLVREIRRRLR 265 P+A+ARL+E IP EK L+ I RL+ Sbjct: 242 PIAAARLSEWIPNEEKALLLNLIEERLK 269 >Q97G02.1 Q97G02_CLOAB Possible aminoglycoside phosphotransferase (Protein kinase related), diverged Length = 284 Score = 203 bits (517), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 101/267 (37%), Positives = 169/267 (63%), Gaps = 9/267 (3%) Query: 3 GKRIGEGRTAEVQEYGTGKILKLYRADIPAEYVEHEYEVSKYVHKQGIQTPQPIELVTLG 62 GK I +G+T+EV EYG KILKL+R D+P + +E+EYEVS + K+ P+ +LV + Sbjct: 23 GKLIAQGKTSEVFEYGQDKILKLFRKDLPEKNIENEYEVSFNLSKKMKLVPKVYDLVEVD 82 Query: 63 ERKGILFQQIHGSSMLRLIGEKPWRLGEYARQLASLHHDLHKLQGPEAFG--KQKESLRS 120 +RKGI++++++G++M+ +I KPW++ + A++LA LH +Q P F K +L+ Sbjct: 83 DRKGIIYEKVNGTTMMEVISSKPWKVKKEAQRLAELH---EAIQKPVDFELPDYKTTLKE 139 Query: 121 SIVAAPMLTMEEKTPVLEKLEQLPEGDRLLHGDFHPDNVLI--DGQAWTIDWMTGMSGNP 178 +I +L + K + + +++L + D L HGDFHPDNVLI +G+ IDWMT G+ Sbjct: 140 NISKTELLEEDIKKRLYDYIDELKDDDLLCHGDFHPDNVLITEEGEV-IIDWMTASKGSR 198 Query: 179 AGDAARSVMMFSMGALPPGASLSTRLVTGFIRQRLTKGYIREYLRLSGLSYAEVNAWVLP 238 D AR+ +M GA P S +++ F+R R GYI+ Y++++G++ ++ W LP Sbjct: 199 IADIARTSVMLKFGASPVKRSAEKKIIK-FVRNRFYSGYIKHYMKITGINKEQIEEWELP 257 Query: 239 VASARLTEGIPLAEKEQLVREIRRRLR 265 +A+ARL E +P +EK L++ I +++ Sbjct: 258 IAAARLNEWLPKSEKVDLLKFIDMKMK 284 >B8I0J4.1 B8I0J4_CLOCE Aminoglycoside phosphotransferase Length = 268 Score = 175 bits (443), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 96/267 (35%), Positives = 156/267 (58%), Gaps = 11/267 (4%) Query: 3 GKRIGEGRTAEVQEYGTGKILKLYRADIPAE-YVEHEYEVSKYVHKQGIQTPQPIELVTL 61 GK +G G TAEV E+G K+LKLY + +V+HE +V VH+ GI +P + V + Sbjct: 4 GKLLGSGVTAEVYEWGEDKVLKLYFNKYSNDDWVDHEAKVGYLVHESGIMSPAVFDKVEI 63 Query: 62 GERKGILFQQIHGSSMLRLIGEKPWRLGEYARQLASLHHDLHKLQGPEAFGKQKESLRSS 121 RKGI+F++I G+S++ + +PW L Y +QLA L +++HK QKE S Sbjct: 64 NGRKGIVFERIFGNSIIERLIIEPWELYNYVQQLAVLQYNIHKFSS-NGLPTQKERFTSK 122 Query: 122 IVAAPMLTMEEKTPVLEKLEQLPEGDRLLHGDFHPDNVLIDGQAWT-IDWMTGMSGNPAG 180 I A+ + +L+ +E LP+G+ + HGD + +NV++ G IDW + +GNP G Sbjct: 123 IRASSSILRGRLKRILDYMESLPDGESICHGDLYFNNVILSGNKLVPIDWNSAYTGNPLG 182 Query: 181 DAARSVMMFSMGALPP---GASLSTRLVTGFIRQRLTKGYIREYLRLSGLSYAEVNAWVL 237 D AR+ ++ PP G ++T + R + Y+ EY++L+ + Y +++AW+L Sbjct: 183 DVARTCLIIR----PPNLSGVPDPMNMLTYYPRLLAHQVYVNEYIKLAKVKYEDIDAWML 238 Query: 238 PVASARLTEGIPLAEKEQLVREIRRRL 264 PVA++RL + IP EK+ L++ I +RL Sbjct: 239 PVAASRLKDNIP-GEKKWLMKLIDKRL 264 >B3K882.1 B3K882_9BACI Aminoglycoside phosphotransferase Length = 268 Score = 168 bits (426), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 96/265 (36%), Positives = 143/265 (53%), Gaps = 3/265 (1%) Query: 3 GKRIGEGRTAEVQEYGTGKILKLYRADIPAEYVEHEYEVSKYVHKQGIQTPQPIELVTLG 62 G IG+GR+AEV E+G + LKLY E+V +E E+ + V + G+ P ++V + Sbjct: 4 GALIGKGRSAEVYEWGESQALKLYYEGYRPEWVTYEAEMGRIVAEAGVPAPSVFDMVDIE 63 Query: 63 ERKGILFQQIHGSSMLRLIGEKPWRLGEYARQLASLHHDLHKLQGPEAFGKQKESLRSSI 122 R G+++++I G SMLR + P++L + AR +A L H H QKE L +I Sbjct: 64 GRTGLVYERITGPSMLRQVQSAPFQLTKCARDMAQLQHATHCCTT-SLLPPQKEKLEHAI 122 Query: 123 VAAPMLTMEEKTPVLEKLEQLPEGDRLLHGDFHPDNVLI-DGQAWTIDWMTGMSGNPAGD 181 + A + E+ + + L+ PEGD + HGDFHPDNV++ + IDW GNP D Sbjct: 123 LDASGILQEKAQAICKVLDSFPEGDSICHGDFHPDNVIVAENGCRAIDWNNAHVGNPLCD 182 Query: 182 AARSVMMFSMGALPPGASLSTRLVTGFIRQRLTKGYIREYLRLSGLSYAEVNAWVLPVAS 241 AR+ +MF P L R K Y+ EY RLS +++ W+LPVA+ Sbjct: 183 VARTSLMFQSPFNPVDQPGILVLPLRMARMLWNKVYLNEYCRLSRAKLEDIDRWMLPVAA 242 Query: 242 ARLTEGIPLAEKEQLVREIRRRLRS 266 ARL E +P E++ L+ I RLR+ Sbjct: 243 ARLREDVP-GERDWLLGLIDDRLRA 266 >B1DCR8.1 B1DCR8_9BACL Aminoglycoside phosphotransferase Length = 264 Score = 167 bits (422), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 96/266 (36%), Positives = 149/266 (56%), Gaps = 7/266 (2%) Query: 2 TGKRIGEGRTAEVQEYGTGKILKLYRADIPAEYVEHEYEVSKYVHKQGIQTPQPIELVTL 61 G+ IG+G TAEV E+G +++KLY +IP +++ +E + V++ G+ +P+ V + Sbjct: 3 AGQLIGKGMTAEVYEWGEAQVVKLYYGNIPKDWLHYESRIGTAVYQAGVPSPEVFGQVEV 62 Query: 62 GERKGILFQQIHGSSMLRLIGEKPWRLGEYARQLASLHHDLHKLQGPEAFGKQKESLRSS 121 R G+++++I G S+L + + Y+ LA LH D+H+ + +QK+ + Sbjct: 63 EGRSGLVYEKIAGQSLLAFMLGDMDQAEHYSILLARLHADIHRCRA-SGLPRQKDRMAMF 121 Query: 122 IVAAPMLTMEEKTPVLEKLEQLPEGDRLLHGDFHPDNVLIDGQAWT-IDWMTGMSGNPAG 180 + + + PVLE LE+LP+G+ + HGD HPDNVL G T IDWM SG+P Sbjct: 122 VRQSASFLGDRVEPVLEALERLPDGESVCHGDLHPDNVLQAGDRLTVIDWMDANSGDPLC 181 Query: 181 DAARSVMMFSMGALPPGASLSTRLVTGFIRQRLTKGYIREYLRLSGLSYAEVNAWVLPVA 240 D AR+ MM L P S+ + QRL + Y+ EY RL+G ++ W LPVA Sbjct: 182 DVARTSMML----LSPFVSMPPLSIPSDTGQRLNEAYLSEYCRLTGTKREAIDRWRLPVA 237 Query: 241 SARLTEGIPLAEKEQLVREIRRRLRS 266 +ARL E IP E+E L+ I R L + Sbjct: 238 AARLRERIP-GEQEWLLTYIDRELEA 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,656,452,883 Number of extensions: 70054942 Number of successful extensions: 189929 Number of sequences better than 1.0e-05: 102 Number of HSP's gapped: 189736 Number of HSP's successfully gapped: 103 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)