BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0019.p (368 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8MKB9.1 A8MKB9_ALKOO Spore germination protein 248 6e-64 B8I8X3.1 B8I8X3_CLOCE Spore germination protein 245 6e-63 Q892K2.1 Q892K2_CLOTE Spore germination protein KB 200 2e-49 B0TA86.1 B0TA86_HELMI Spore germination protein kb, putative 198 8e-49 Q0AXQ3.1 Q0AXQ3_SYNWW Putative uncharacterized protein 189 4e-46 >A8MKB9.1 A8MKB9_ALKOO Spore germination protein Length = 366 Score = 248 bits (634), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 127/354 (35%), Positives = 215/354 (60%) Query: 1 MSKEIIPAGQAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGL 60 M+KE+I Q ISI +LF+IGT+L G + ++ +W Y+R+ L Sbjct: 1 MNKEVISTRQGISIMILFLIGTTLVAGGANKAQQDAWISISFAILMAIPLCFMYSRITTL 60 Query: 61 FPGKDLYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPML 120 GK+L++++ ++FG + G++++ LY WY H+GS+V+RN EF + V+ TP + Sbjct: 61 LYGKNLFEIVEILFGRILGKIITILYTWYFFHIGSLVIRNITEFIQVVSFPDTPQPFVAI 120 Query: 121 VIGLLCVWVVKAGMEVLGRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVL 180 +GL+ ++++K+G+E LGR +F+L + + +I LS PK +L P+L +G+K V Sbjct: 121 FMGLVVIYMIKSGIEGLGRWTEFVLPVVLFTLIIIIFLSAPKVNLMNLRPILYNGFKPVF 180 Query: 181 SDTIGSFTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDI 240 + FPFAE +VF F+ L + K+ S +IAG++I +++RN++VLG Sbjct: 181 QGAVSLIIFPFAETIVFTVVFSSLQNHRKSFKIYFVSILIAGLVIFSISIRNILVLGASS 240 Query: 241 LSSLYFPSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSY 300 + +LYFPSY AV I+IGDFL RIE ++ + + K+S+ L+A S G+A++F L +Y Sbjct: 241 IGTLYFPSYSAVSLIDIGDFLQRIEVIVSVTLIVSSIAKLSVFLFATSLGIAQIFNLKNY 300 Query: 301 RSVVLQLGLIMVYLAVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEI 354 S+ + L+M+ L++ IY M+M ++ Y++PFQ+ IP+ +W+TAEI Sbjct: 301 TSIATPICLLMINLSIIIYGSTMEMFHWIERFLPYYSIPFQLIIPLCIWITAEI 354 >B8I8X3.1 B8I8X3_CLOCE Spore germination protein Length = 368 Score = 245 bits (625), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 137/368 (37%), Positives = 218/368 (59%), Gaps = 2/368 (0%) Query: 1 MSKEIIPAGQAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGL 60 MSKE+I QA +I ++F +G++L +G+ G + N W Y ++ Sbjct: 1 MSKELITQKQASAIMIMFALGSTLVLGAGGAAKNDVWIAILISLLMSVPIMLLYCKIQMS 60 Query: 61 FPGKDLYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPML 120 +P K++Y++ FG V G+++S ++IWY+ HLGS+V+RNF EF V+L TP L Sbjct: 61 YPNKNIYEIFDNAFGKVIGKIMSLMFIWYSFHLGSLVIRNFTEFIVNVSLPKTPQNIVGL 120 Query: 121 VIGLLCVWVVKAGMEVLGRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVL 180 + LC+W VK+G+EV+ R + T+ V+ V+ +L +P + + +L PVL G V+ Sbjct: 121 FMVFLCIWAVKSGIEVISRWTAIMFPVTLFVVFVVTILFVPLFNFTNLKPVLYYGMGPVI 180 Query: 181 SDTIGSFTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDI 240 + F FPFAE V+FL L ++ KV S +I+G+ L+V++R L+ LG Sbjct: 181 NTAFSVFAFPFAETVIFLAVLGNLREQRGVYKVYFFSLLISGISTLMVSVRTLLTLGVPN 240 Query: 241 LSSLYFPSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSY 300 +S ++FP+Y +V I+IGDFL RIE + A++F+ A FIK+S+CLY+ S G+A + L SY Sbjct: 241 ISVIFFPTYASVRLIDIGDFLQRIEITVAMVFLFAGFIKISICLYSTSIGIAHLMGLSSY 300 Query: 301 RSVVLQLGLIMVYLAVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRS 360 R VV LGL M L++ +Y + ++M ++ + YK YA FQV +PVI ++ E A + Sbjct: 301 RQVVAVLGLAMSVLSIVVYSNTLEMFDWSDNFYKYYAFIFQVILPVITFIAIE--ARKIY 358 Query: 361 GKKTAAQQ 368 +K Q Sbjct: 359 SRKKKLQH 366 >Q892K2.1 Q892K2_CLOTE Spore germination protein KB Length = 369 Score = 200 bits (509), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 104/368 (28%), Positives = 197/368 (53%), Gaps = 3/368 (0%) Query: 1 MSKEIIPAGQAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGL 60 M KE+I Q + + IG L W Y ++ L Sbjct: 5 MKKEVISNKQIQLLIFSYCIGAYLLFSMGAGIKQDIWISSILAIIFTIPIVIMYGKIMNL 64 Query: 61 FPGKDLYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPML 120 +PGK+ +D+L VFG + G++L+ ++I++A LG+ +LR+F +F K AL STPM+ PM+ Sbjct: 65 YPGKNFFDILEEVFGRMVGKVLNIIFIFHAFFLGAYILRDFADFIKVTALFSTPMIVPMI 124 Query: 121 VIGLLCVWVVKAGMEVLGRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVL 180 IG+L +W++ G+EVL AKFL+ ++ + +I ++ IP+ +L PV + K +L Sbjct: 125 CIGILSIWILTEGIEVLAAWAKFLIRIILISMILIWIMLIPEMHISNLQPVFYADLKDIL 184 Query: 181 SDTIGSFTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDI 240 + TFPF+E+++FL F+ + V + ++ G+++++ T+ NLM+LG ++ Sbjct: 185 KQALHLITFPFSEVIIFLNFFDYVNYNSKTKNVFIKPLILGGMLVVLYTMINLMLLGGEL 244 Query: 241 LSSLYFPSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSY 300 S Y+P Y ++ R+N+G+ R+E +I F F++++ C+ S G+ KVF L Y Sbjct: 245 YSFFYYPGYESIKRLNLGNEFQRLEIIVSIAFTIIQFLEINFCILGVSKGITKVFNLKEY 304 Query: 301 RSVVLQLGLIMVYLAVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRS 360 R V++ + ++++ ++ M+ ++ Y + Q+ +P I+++ A I +R Sbjct: 305 RRVLIPVIILLITFDYVMFGSAMEAFEIVRELWPAYGIVIQIVLPFIIFIFATI---KRK 361 Query: 361 GKKTAAQQ 368 + + Sbjct: 362 NQLVKCNK 369 >B0TA86.1 B0TA86_HELMI Spore germination protein kb, putative Length = 377 Score = 198 bits (503), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 127/368 (34%), Positives = 192/368 (52%), Gaps = 6/368 (1%) Query: 3 KEIIPAGQAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGLFP 62 KE + Q I + VLFIIG+SL + ++ W Y R+ L Sbjct: 10 KEQVSFKQGIYLIVLFIIGSSLILPIGAKAKQDLWIAALLALVFALPMVVVYGRILSLNA 69 Query: 63 GKDLYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPMLVI 122 G DL ++ +FG + G+ + +Y +YA L +VL NFGEF VA+ TP L M + Sbjct: 70 GMDLLEIATRLFGTIIGKAIILVYAFYAFTLSGLVLINFGEFISVVAIPETPKLVIMFFL 129 Query: 123 GLLCVWVVKAGMEVLGRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVLSD 182 LL +W VK G+EV+ R A+ + I+ V LL PK++ P+L + + Sbjct: 130 ILLAIWAVKEGIEVIARMAEIYFPWIIISAIVTFLLLSPKFEIQRFYPILYQNHQKIFQA 189 Query: 183 TIGSFTFPFAEIVVFLGAFNVLP--KKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDI 240 I F+FP AE+V+FL +LP K+ S K + ++ + ++V+G + Sbjct: 190 AIDLFSFPLAEMVLFL---LLLPSSKQASVVKQYMLGLLLGFFAVFTSITTVMVVVGSEQ 246 Query: 241 LSSLYFPSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSY 300 + YFP ++AV +I+IGDFL R+E +I + A FIK+ +CL AS G+ VF Y Sbjct: 247 YGAHYFPGFLAVQKISIGDFLQRLEVVIFLITLFAGFIKICVCLLGASRGICHVFGFADY 306 Query: 301 RSVVLQLGLIMVYLAVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRS 360 R +V +G + + A+ IY QM +A IY YA PFQV +P+++W+ AEI A R Sbjct: 307 RFLVTPVGFLSLLFALNIYSSTKQMARWADAIYPYYAFPFQVLLPLLIWIVAEIKA-RAE 365 Query: 361 GKKTAAQQ 368 GKK A + Sbjct: 366 GKKGAPAK 373 >Q0AXQ3.1 Q0AXQ3_SYNWW Putative uncharacterized protein Length = 366 Score = 189 bits (480), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 118/349 (33%), Positives = 191/349 (54%), Gaps = 7/349 (2%) Query: 18 FIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGLFPGKDLYDMLILVFGAV 77 F+ G S+ + + + +W + L F GK L ++ LV+G + Sbjct: 20 FVFGASVIIAPGQGAEHDAWIAIALGLMEGLIIAGIFTALARHFKGKTLVEINSLVYGKI 79 Query: 78 AGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPMLVIGLLCVWVVKAGMEVL 137 G +S L+IWY HLG++VL N+ F K +TP +++I L+C V+ G+EVL Sbjct: 80 LGNFISILFIWYLFHLGALVLNNYSRFLKLELYPATPKTLTLILIILVCASTVRRGIEVL 139 Query: 138 GRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVLSDTIGSFTFPFAEIVVF 197 R + L+ TI++ LL +P H+L+P+LD +L + G+ FPF + V F Sbjct: 140 ARCSLILVTLTIIMAIFDTLLVVPHMVLHNLMPILDIPLGKLLWSSHGTAVFPFGDAVAF 199 Query: 198 LG--AFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDILSSLY-FPSYVAVGR 254 L AF P+KG +A + MIAG++++ V +RN VLG ++++Y +P+Y+ Sbjct: 200 LMVLAFVNKPEKGRSA--VFKGIMIAGLVLIGVAVRNAAVLGQ--MNAIYTYPTYITAQV 255 Query: 255 INIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSYRSVVLQLGLIMVYL 314 I++GD LTR+E AI +T F K+S+ Y G+A+VF L SYR ++L +G++MV L Sbjct: 256 IDVGDVLTRVEVLVAINLITMGFFKISVAYYGTVLGLAQVFNLQSYRPLILPIGILMVIL 315 Query: 315 AVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRSGKK 363 A+ + +M FA Y IY +PFQ+AIP+I A++ + G + Sbjct: 316 ALTNVSNTAEMFDFANQGYPIYTVPFQIAIPLITLFVAKLRKLPKIGGE 364 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,893,890,971 Number of extensions: 71143490 Number of successful extensions: 289207 Number of sequences better than 1.0e-05: 456 Number of HSP's gapped: 288768 Number of HSP's successfully gapped: 456 Length of query: 368 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 231 Effective length of database: 1,770,017,148 Effective search space: 408873961188 Effective search space used: 408873961188 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)