BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0017.p (384 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8MKB8.1 A8MKB8_ALKOO Spore germination B3 GerAC family protein 252 6e-65 B8I8X5.1 B8I8X5_CLOCE Germination protein, Ger(X)C family 224 9e-57 B0TA88.1 B0TA88_HELMI Spore germination protein b3, gerac, putative 213 3e-53 Q24NX6.1 Q24NX6_DESHY Putative uncharacterized protein 212 6e-53 B8FX22.1 B8FX22_DESHD Germination protein, Ger(X)C family 212 6e-53 >A8MKB8.1 A8MKB8_ALKOO Spore germination B3 GerAC family protein Length = 384 Score = 252 bits (643), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 134/374 (35%), Positives = 222/374 (59%), Gaps = 2/374 (0%) Query: 13 CIVMLPCLSGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQNQAGFKML 72 I+ + LSGCWNYTE+D I+IV+GVAIDK K +TAE+V+IQG L Q + + Sbjct: 11 IIIPMTILSGCWNYTEIDKIAIVSGVAIDKGREGNKFMITAEIVEIQGSLTQGNVKARRI 70 Query: 73 SLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTETRSDVFI 132 G T+FD +RN++ I+ KKL+W+H KA++ S++VA+E LI +D+ SRD +TR + + Sbjct: 71 QAEGETLFDAIRNILRISAKKLYWNHTKAVVLSQEVAKEDLIHILDFLSRDNQTRLTLNL 130 Query: 133 FVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLETMGNHAVA 192 FVS E TA E+ S T I FE+ M+ S + +++ + ID + + G Sbjct: 131 FVSREKTAWELFEQQSITTDIRGFEMDYMLISNNNLSKSGAMDMIELIDTISSEGISPYL 190 Query: 193 PLIYIHEQNGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGVNDKNGVP 252 P I I + +K +N A+F KDK++G L+G +TK LFAKD IKGGVL ++ + Sbjct: 191 PSIGITLNDEKKTSELNSIAVFQKDKLLGFLDGSDTKFFLFAKDRIKGGVLMISPEESHQ 250 Query: 253 TY--SLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAIEERAGE 310 + +LEIL++ TK++P ++ KL +++ T + E + E S ++ A + Sbjct: 251 DHKVTLEILNNTTKIRPVYLDEKLTMKIDIHTEVVIGENDFLIDYINREGRSQLKAAAEQ 310 Query: 311 ALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKLDITVDSKVII 370 L++++ VI K+Q+++ +DI G+G II + P +W ++ +WD+ F + + V ++ I Sbjct: 311 QLKEDVQNVIHKIQKDFGSDILGFGRIIKAYHPSLWKNIKYQWDELFKTVAVDVHVELDI 370 Query: 371 ESTAKTSRSIKMGD 384 + S I++G+ Sbjct: 371 IGSGINSTPIRVGE 384 >B8I8X5.1 B8I8X5_CLOCE Germination protein, Ger(X)C family Length = 380 Score = 224 bits (572), Expect = 9e-57, Method: Compositional matrix adjust. Identities = 121/364 (33%), Positives = 206/364 (56%), Gaps = 3/364 (0%) Query: 21 SGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQNQAGFKMLSLTGSTIF 80 +GCWNY E++ +SIVAG AIDK N K +++ E++D++ G + + K + G ++ Sbjct: 20 TGCWNYREINQMSIVAGAAIDK-APNGKYKVSIEIIDLKTGGTEAKVKTKKVETYGDSVV 78 Query: 81 DIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTETRSDVFIFVSGEPTA 140 D VRN IS L+W H + II S+ +A EG+++ +D +SRD E R + I VS E TA Sbjct: 79 DAVRNAISFNANTLYWGHTEIIIISKDIAEEGIVQILDCFSRDAEPRLSMDILVSKEDTA 138 Query: 141 REILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLETMGNHAVAPLIYIHEQ 200 EIL+ S T + +FE+ +++ EK + VEI+ FI+ L G + P+I + Sbjct: 139 EEILDSQSITTEVRTFEINKILDIEKRLGKSLKVEIYQFINALGETGISPIMPVIGLTIN 198 Query: 201 NGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGVNDKNGVPTYSLEILS 260 + +K ++G A+F DK+ ++T+ LF + I GVL VN+ + +L I+ Sbjct: 199 SEEKTSELSGTAVFKGDKLAYMFGQEDTQYFLFVNNKIHEGVLVVNNDDD--NITLRIIK 256 Query: 261 SRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAIEERAGEALEQEILAVI 320 ++TK+KP NGKL + T L E+ S E I I++ A + L+ ++ +VI Sbjct: 257 NKTKLKPVYNNGKLHFDIYIKTKVALSEINKSTKLENVEDIDKIQKIAEKYLKNKVESVI 316 Query: 321 RKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKLDITVDSKVIIESTAKTSRSI 380 + +Q EY DIFG+G + + P +W ++ WD+ F + + V+ ++ ++++ S+ I Sbjct: 317 KAVQNEYGIDIFGFGRSVRQDFPNLWKEINPDWDNVFKNVPVNVNVEIQVKNSGLLSKKI 376 Query: 381 KMGD 384 +GD Sbjct: 377 VIGD 380 >B0TA88.1 B0TA88_HELMI Spore germination protein b3, gerac, putative Length = 379 Score = 213 bits (541), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 118/381 (30%), Positives = 214/381 (56%), Gaps = 13/381 (3%) Query: 13 CIVMLPCLSGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQNQAGF--- 69 C+ ++ +GCWNY E+ +++IV G A DK +E I LT E++ + G +G Sbjct: 3 CLCLILPSAGCWNYREIRELAIVVGAAFDKQ--DEGIHLTVELMSTKTGEKTEGSGTLGI 60 Query: 70 --KMLSLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTETR 127 + G T+F+ VRNMI+ TGK+L+WSHAKA I SE++A++G+I+ +DW +RD E R Sbjct: 61 RPQRFESYGKTVFEAVRNMIAKTGKRLYWSHAKAFIVSEEMAKDGMIQLIDWINRDAEVR 120 Query: 128 SDVFIFVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVE--IWDFIDKLET 185 +I V+ A +IL S +S +L + +R +Y++S + + DF++++E+ Sbjct: 121 LQTWILVTRGEKASDILKTKSKLFDTVSMQLEEALR--RYSNSGKLLSSTVGDFVEEMES 178 Query: 186 MGNHAVAPLIYIHEQNGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIK-GGVLG 244 G A APL+ E G K ++ G A+F +++VG L+ +T+++LF +D +K GG++ Sbjct: 179 PGIGATAPLVQTAENAGDKIPQIGGTALFRGERLVGYLDADQTRNLLFIRDRMKGGGLIA 238 Query: 245 VNDKNGVPTYSLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAI 304 ++ + SLEI S TK + V + ++++ + E+ T + E + Sbjct: 239 ISTEEPKTKVSLEIYRSDTKRQLHVEGHRPVIELTVEPLADIAEISTEKDVISLEKKEEL 298 Query: 305 EERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDR-WDDEFAKLDIT 363 E+A + L +E+ IR++Q +Y D+FG+G++I+ +P++W + W F L++ Sbjct: 299 TEKANKQLARELQETIREVQEKYQTDVFGFGQMIYRRRPDLWEAIEQTGWGRLFPDLEVR 358 Query: 364 VDSKVIIESTAKTSRSIKMGD 384 ++ +A S K+G+ Sbjct: 359 TIVNFKLKGSALLSEPTKVGE 379 >Q24NX6.1 Q24NX6_DESHY Putative uncharacterized protein Length = 389 Score = 212 bits (539), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 113/365 (30%), Positives = 210/365 (57%), Gaps = 13/365 (3%) Query: 13 CIVMLPCLSGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQ-------GGLDQN 65 C ++ ++GCWN E++ ++IV G+ IDK +IQ+TA++ + + GG D Sbjct: 14 CTPLILNITGCWNQRELNSLAIVMGLGIDKAQEPNQIQVTAQIANPKALKSSSLGGPDSQ 73 Query: 66 QAGFKMLSLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTE 125 F + TG T+F I+R+ + +KL++ H + +I A EG+ + +D+++RD E Sbjct: 74 --AFWNIQNTGDTLFSILRDFTHESNRKLYFPHIQVLILGRDYAEEGIQKELDFFARDHE 131 Query: 126 TRSDVFIFVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLET 185 TR ++++ VS E AR++LN+ I + +++++ D+ S T ++ DF+ L + Sbjct: 132 TRLNIWLVVS-EGNARDVLNIKPVIDRIPAINISKLLDDQDPNSQTSTSQLNDFLSDLIS 190 Query: 186 MGNHAVAPLIYIHEQNGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGV 245 APL+ I + G ++ +V G A+F DK+VG+LN ET+ +L+A+D +K G++ V Sbjct: 191 PTTAPTAPLVEIIRKGGNESLQVKGTAVFKGDKLVGELNKYETRGLLWARDEVKSGIIDV 250 Query: 246 NDKNGVPTYSLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAIE 305 SLEI+ S+TKV +V+ +++++V L +E+ E ++AIE Sbjct: 251 ECPECGSKVSLEIIDSKTKVSAEIVDNQVIMKVYIQEKGALAAQYCAENLRTKEKVAAIE 310 Query: 306 ERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKL--DIT 363 A++ E+LA +K Q E +ADIFG+GE++ + + W +++ WD+ F ++ ++T Sbjct: 311 RNKAAAIQDEVLAAFKKAQ-ELNADIFGFGEVVRKKHLKEWKNMQEHWDEIFPEIIVEVT 369 Query: 364 VDSKV 368 VD+ + Sbjct: 370 VDASI 374 >B8FX22.1 B8FX22_DESHD Germination protein, Ger(X)C family Length = 389 Score = 212 bits (539), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 113/365 (30%), Positives = 210/365 (57%), Gaps = 13/365 (3%) Query: 13 CIVMLPCLSGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQ-------GGLDQN 65 C ++ ++GCWN E++ ++IV G+ IDK +IQ+TA++ + + GG D Sbjct: 14 CTPLILNITGCWNQRELNSLAIVMGLGIDKAQEPNQIQVTAQIANPKALKSSSLGGPDSQ 73 Query: 66 QAGFKMLSLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTE 125 F + TG T+F I+R+ + +KL++ H + +I A EG+ + +D+++RD E Sbjct: 74 --AFWNIQNTGDTLFSILRDFTHESNRKLYFPHIQVLILGRDYAEEGIQKELDFFARDHE 131 Query: 126 TRSDVFIFVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLET 185 TR ++++ VS E AR++LN+ I + +++++ D+ S T ++ DF+ L + Sbjct: 132 TRLNIWLVVS-EGNARDVLNIKPVIDRIPAINISKLLDDQDPNSQTSTSQLNDFLSDLIS 190 Query: 186 MGNHAVAPLIYIHEQNGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGV 245 APL+ I + G ++ +V G A+F DK+VG+LN ET+ +L+A+D +K G++ V Sbjct: 191 PTTAPTAPLVEIIRKGGNESLQVKGTAVFKGDKLVGELNKYETRGLLWARDEVKSGIIDV 250 Query: 246 NDKNGVPTYSLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAIE 305 SLEI+ S+TKV +V+ +++++V L +E+ E ++AIE Sbjct: 251 ECPECGSKVSLEIIDSKTKVSAEIVDNQVIMKVYIQEKGALAAQYCAENLRTKEKVAAIE 310 Query: 306 ERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKL--DIT 363 A++ E+LA +K Q E +ADIFG+GE++ + + W +++ WD+ F ++ ++T Sbjct: 311 RNKAAAIQDEVLAAFKKAQ-ELNADIFGFGEVVRKKHLKEWKNMQEHWDEIFPEIIVEVT 369 Query: 364 VDSKV 368 VD+ + Sbjct: 370 VDASI 374 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,052,202,393 Number of extensions: 82591995 Number of successful extensions: 202740 Number of sequences better than 1.0e-05: 444 Number of HSP's gapped: 201968 Number of HSP's successfully gapped: 445 Length of query: 384 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 247 Effective length of database: 1,770,017,148 Effective search space: 437194235556 Effective search space used: 437194235556 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)