BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0016.p (490 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I8X6.1 B8I8X6_CLOCE GerA spore germination protein 501 e-140 A8MKB7.1 A8MKB7_ALKOO GerA spore germination protein 469 e-130 B0TA89.1 B0TA89_HELMI Spore germination protein ka 458 e-127 Q892K0.1 Q892K0_CLOTE Spore germination protein KA 407 e-111 Q97TS7.1 Q97TS7_CLOAB Spore germination protein, GRKA 389 e-106 >B8I8X6.1 B8I8X6_CLOCE GerA spore germination protein Length = 519 Score = 501 bits (1289), Expect = e-140, Method: Compositional matrix adjust. Identities = 230/486 (47%), Positives = 348/486 (71%), Gaps = 4/486 (0%) Query: 1 MEDNLARVRQIFSNDGTLRVRFIENNHGTPLRCCLIYVEGMIDRTLLQNGITKPVMAYPF 60 + N+ R IF +D T VR +EN H ++CC++++E MID T++ I +P+M Sbjct: 37 LSKNIDLFRNIFRDDDTFIVRHVENQHNKSIKCCVLFMEEMIDTTIVNENILRPIM---- 92 Query: 61 KEEDCGNSAELIEKLRAEIIALSDITVTTGLDEVVGGIVGGNTLLLLDGYAGALSISTQG 120 ++ N+ + + + + +I +I ++ ++ + I+ G+T+LLL+ AL IS+ G Sbjct: 93 QDSTLKNNNDTLVQFQNRVITSDNIKRSSDVNLLTNSILKGDTVLLLEDCYEALIISSIG 152 Query: 121 LETRAIEEPTTEKAVRGPREGFTESLFVNLTLIRRKVQNPDLKFVFRDIGSRTKTQTCIC 180 +++RAI+EP EK +RGPREGFTE L +NL+L+R++++ PDLKF F +G++T T+ CIC Sbjct: 153 VKSRAIQEPEAEKLIRGPREGFTEPLMINLSLLRKRLETPDLKFKFLTLGTKTNTKICIC 212 Query: 181 YMEGLASPEILAELQGRLDRVEIDHVLDTGYLAELIRDEPYSPFEMLGSTERPDAVVGKI 240 Y++ L + +IL E++ RL ++ID +L +GY+ ELI DEPYSPF+ +GSTERPD VVGK+ Sbjct: 213 YLDSLVNRKILNEVETRLRSIKIDGILASGYIGELINDEPYSPFKTVGSTERPDVVVGKL 272 Query: 241 MEGRIALLIEGSPFALTLPYVFVENFQAAEDYYINYYFASFNRFLRVMGAFMSISIPAAY 300 +EGRIA++++G+P ALT+PYVF+E FQ EDYYINYYF+S +R LR++G +++S+PA Y Sbjct: 273 LEGRIAIVVDGTPVALTVPYVFIEYFQINEDYYINYYFSSISRILRILGFILTVSVPAIY 332 Query: 301 VALVTYSQEMVPTLLLLSIATARLSVPFPTVVEALIMLTIFEILREAGARIPTSIGQAVS 360 VAL+T+ QEM+PT LL+SI+++R VPFPT+VE +++L +FEILRE G R+PT+IGQA+S Sbjct: 333 VALMTFQQEMIPTPLLISISSSRQGVPFPTIVETMLLLIVFEILRETGTRMPTNIGQALS 392 Query: 361 IVGALVLGQAAVDARIVSAPMVIVVGLTGITTLLNPRLTGPMIIVRXXXXXXXXXXXXXX 420 IVGALVLGQA+V A+IVSAP+VIVV ++GIT+L+ P++ G I++R Sbjct: 393 IVGALVLGQASVQAKIVSAPVVIVVAISGITSLMIPKIQGAAIVLRVIFLLLSAFLGFYG 452 Query: 421 XXXXXXXXVIHLMSLRSFGVPYMLGVGSIRPQDIKDTAIRAPWWDMYLRPAVMGARNMKR 480 ++HL +RSFGVPY+L + S+ P D+KDT IRAP W M RP ++ NM R Sbjct: 453 LIFGITGTLLHLCEMRSFGVPYLLYLTSMNPYDLKDTIIRAPLWKMKKRPKLISTDNMMR 512 Query: 481 KLPGKR 486 ++ G++ Sbjct: 513 QVIGRK 518 >A8MKB7.1 A8MKB7_ALKOO GerA spore germination protein Length = 496 Score = 469 bits (1207), Expect = e-130, Method: Compositional matrix adjust. Identities = 221/485 (45%), Positives = 328/485 (67%), Gaps = 4/485 (0%) Query: 2 EDNLARVRQIFSNDGTLRVRFIENNHGTPLRCCLIYVEGMIDRTLLQNGITKPVMAYPFK 61 + N+ +++IF +D TL R +N + T L+C +++++GM++ + I KP++ Sbjct: 14 DKNIEMIKEIFDHDATLVSRLFQNQNNTCLKCGIVFIDGMVNDGHINEDIIKPILTNAV- 72 Query: 62 EEDCGNSAELIEKLRAEIIALSDITVTTGLDEVVGGIVGGNTLLLLDGYAGALSISTQGL 121 N A+L ++L+ EII+ S+++ T +++++ IV G+T+L L+ AL I TQ Sbjct: 73 ---VKNKADLFDQLQYEIISASNVSRTDDVNKIIEEIVSGSTVLFLENANSALIIDTQQY 129 Query: 122 ETRAIEEPTTEKAVRGPREGFTESLFVNLTLIRRKVQNPDLKFVFRDIGSRTKTQTCICY 181 ETR+IEEP +EK +RGPREGFTE++ N++++RRK++ LK FR G ++ T+TCICY Sbjct: 130 ETRSIEEPVSEKTLRGPREGFTENILTNISMVRRKLKTNHLKLEFRVFGKQSHTKTCICY 189 Query: 182 MEGLASPEILAELQGRLDRVEIDHVLDTGYLAELIRDEPYSPFEMLGSTERPDAVVGKIM 241 ++G+ + +IL EL RL+ +D VLD Y+ E I+D P S F+ G+TERPD V K++ Sbjct: 190 IDGIVNKKILQELYRRLEDFHMDGVLDVNYIQEFIKDSPLSLFDTTGTTERPDVVAAKLL 249 Query: 242 EGRIALLIEGSPFALTLPYVFVENFQAAEDYYINYYFASFNRFLRVMGAFMSISIPAAYV 301 EGRIA++I+GSP LT+P++F+E FQ+ EDYY+++YF+S R LRV +ISIPA Y+ Sbjct: 250 EGRIAIIIDGSPVVLTVPFLFIEYFQSCEDYYVSFYFSSIGRLLRVFSFIFTISIPAIYL 309 Query: 302 ALVTYSQEMVPTLLLLSIATARLSVPFPTVVEALIMLTIFEILREAGARIPTSIGQAVSI 361 +LVT+ QE++PT LLLSI+ AR VPFPTV+E + +L IFEILRE+G R+P +G+++SI Sbjct: 310 SLVTFHQELIPTNLLLSISAARQGVPFPTVIELVALLIIFEILRESGTRMPIPMGESLSI 369 Query: 362 VGALVLGQAAVDARIVSAPMVIVVGLTGITTLLNPRLTGPMIIVRXXXXXXXXXXXXXXX 421 VGALVLGQAAV+AR V AP+VI+V +T IT L+ ++ G I +R Sbjct: 370 VGALVLGQAAVEARFVGAPIVIIVSITAITGLMISQIKGASITLRFAFLLISATLGLYGY 429 Query: 422 XXXXXXXVIHLMSLRSFGVPYMLGVGSIRPQDIKDTAIRAPWWDMYLRPAVMGARNMKRK 481 +I L +RSFG+PYM V SI Q++KDTAIRAPWW M RP V+ +N++RK Sbjct: 430 IFAIATLLISLFKIRSFGIPYMSNVTSIHLQELKDTAIRAPWWYMKYRPKVLAYKNIQRK 489 Query: 482 LPGKR 486 G R Sbjct: 490 STGGR 494 >B0TA89.1 B0TA89_HELMI Spore germination protein ka Length = 508 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 227/488 (46%), Positives = 322/488 (65%), Gaps = 11/488 (2%) Query: 2 EDNLARVRQIFSNDGTLRVRFI----ENNHGTPLRCCLIYVEGMIDRTLLQNGITKPVMA 57 ++N+ +++I +ND + R + ++NH RCC++YV+GM+D LL + + Sbjct: 25 DENMRILKKILANDDLIIYRPLTLEGQDNH----RCCVVYVDGMVDSDLLHENVLTRLQD 80 Query: 58 YPFKEEDCGNSAELIEKLRAEIIALSDITVTTGLDEVVGGIVGGNTLLLLDGYAGALSIS 117 +++ EL+ II+ ++T + + ++ GN LL+LD + I Sbjct: 81 VRWQQVP---DDELLSLFVKRIISAGNVTTVAKVRPAIQALLYGNVLLVLDSADAVIQIE 137 Query: 118 TQGLETRAIEEPTTEKAVRGPREGFTESLFVNLTLIRRKVQNPDLKFVFRDIGSRTKTQT 177 +G E RAI EP TE+ VRGPREGF E++ NL +IRRKV NP+LK V+R+IG+RTKT+ Sbjct: 138 AKGWEKRAISEPETERVVRGPREGFIENIVTNLAMIRRKVPNPNLKMVYREIGTRTKTKI 197 Query: 178 CICYMEGLASPEILAELQGRLDRVEIDHVLDTGYLAELIRDEPYSPFEMLGSTERPDAVV 237 CI Y+EG++ E+L ELQ RLD+V++D +L+ Y+ ELIRD P SPFE LG+TERPD V Sbjct: 198 CIVYLEGVSRKELLEELQRRLDKVQLDGILEAEYIIELIRDAPLSPFETLGTTERPDTAV 257 Query: 238 GKIMEGRIALLIEGSPFALTLPYVFVENFQAAEDYYINYYFASFNRFLRVMGAFMSISIP 297 GK++EGRI ++++GSPF +TLPY+F+E FQA +DYY N+Y+++ NR LR + F++ S P Sbjct: 258 GKLLEGRILIVVDGSPFVITLPYLFIEYFQANKDYYENFYYSTVNRLLRYLSFFLTTSTP 317 Query: 298 AAYVALVTYSQEMVPTLLLLSIATARLSVPFPTVVEALIMLTIFEILREAGARIPTSIGQ 357 A +VAL + QEM+PT LL SI+ A+ VPFPTVVEA +MLT F+ILREAG R+P+ +GQ Sbjct: 318 AIFVALTAFHQEMIPTRLLFSISAAKEGVPFPTVVEAFLMLTAFQILREAGIRLPSPVGQ 377 Query: 358 AVSIVGALVLGQAAVDARIVSAPMVIVVGLTGITTLLNPRLTGPMIIVRXXXXXXXXXXX 417 AVSIVGALVLG AAV A+I+SAPMVIV +TGIT+ + P + G ++ Sbjct: 378 AVSIVGALVLGDAAVTAKIISAPMVIVAAVTGITSFIVPEMPGVAFLLSLFFLFAASFLG 437 Query: 418 XXXXXXXXXXXVIHLMSLRSFGVPYMLGVGSIRPQDIKDTAIRAPWWDMYLRPAVMGARN 477 IHL +RSFGV Y+ + SIR QD+KDTAIRAPWW M RP ++ + Sbjct: 438 LFGYIFAVIALTIHLAGIRSFGVNYLDSITSIRLQDLKDTAIRAPWWWMTYRPRLLTPND 497 Query: 478 MKRKLPGK 485 + PG+ Sbjct: 498 KRLADPGR 505 >Q892K0.1 Q892K0_CLOTE Spore germination protein KA Length = 518 Score = 407 bits (1045), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/477 (41%), Positives = 301/477 (63%), Gaps = 5/477 (1%) Query: 10 QIFSNDGTLRVRFIENNHGTPLRCCLIYVEGMIDRTLLQNGITKPVMAYPFKEEDCGNSA 69 IF D T+ R +N + + L+ C+I+++GM++ ++ I +M + S Sbjct: 43 NIFPGDNTIIYRQFKNKN-SQLKFCVIFIDGMVNTEIINENILPQLMNTTVNSK----SK 97 Query: 70 ELIEKLRAEIIALSDITVTTGLDEVVGGIVGGNTLLLLDGYAGALSISTQGLETRAIEEP 129 LI ++ E++ ++++ + L++++ ++ G ++L +DG+ L ++T E R+IEEP Sbjct: 98 NLINCIKEEVLIVNEVKESNRLEDILSSLLMGKSILFIDGFEKVLILNTIQWEKRSIEEP 157 Query: 130 TTEKAVRGPREGFTESLFVNLTLIRRKVQNPDLKFVFRDIGSRTKTQTCICYMEGLASPE 189 E V+GPREGF ESL N++LIRR++++ +LK F++IG RTKT CI YM+ + +P+ Sbjct: 158 QGESVVKGPREGFNESLITNISLIRRRIKSQELKLQFKEIGVRTKTTICIAYMDDIVNPK 217 Query: 190 ILAELQGRLDRVEIDHVLDTGYLAELIRDEPYSPFEMLGSTERPDAVVGKIMEGRIALLI 249 IL EL+ RL++++I+ V TG + ELIRD P SPF +G+TERPD V K+++GRIA+L Sbjct: 218 ILKELENRLNKIDIEGVFSTGTMQELIRDNPMSPFNTIGNTERPDVVASKLLQGRIAILC 277 Query: 250 EGSPFALTLPYVFVENFQAAEDYYINYYFASFNRFLRVMGAFMSISIPAAYVALVTYSQE 309 EGSP LTLPY+F+E FQ EDYY N+ +AS NR LR++ ++I+IPA YVA T+ +E Sbjct: 278 EGSPTVLTLPYLFIEQFQVNEDYYDNFIYASVNRILRIIAFILTITIPAIYVAFTTFHKE 337 Query: 310 MVPTLLLLSIATARLSVPFPTVVEALIMLTIFEILREAGARIPTSIGQAVSIVGALVLGQ 369 M+P L SI AR VP PT +EA++M+ FEI+REAG R+P IG VSIVGALVLG Sbjct: 338 MIPIKLAFSIYLAREGVPLPTSIEAILMVITFEIIREAGIRLPKHIGSTVSIVGALVLGD 397 Query: 370 AAVDARIVSAPMVIVVGLTGITTLLNPRLTGPMIIVRXXXXXXXXXXXXXXXXXXXXXXV 429 AAV+A+ VSAP+VIV + GI+ L+ L ++I R V Sbjct: 398 AAVNAKFVSAPIVIVAAIAGISELVLYELRTAIVIWRFIFLILASILGLYGVIFAGMALV 457 Query: 430 IHLMSLRSFGVPYMLGVGSIRPQDIKDTAIRAPWWDMYLRPAVMGARNMKRKLPGKR 486 I LMS+++FG+PYML + + +I D IRAPWW + R + ++ K KR Sbjct: 458 IQLMSIKTFGIPYMLKLIDLDKYNIVDATIRAPWWLLKYRTKFISKDRVRLKDYIKR 514 >Q97TS7.1 Q97TS7_CLOAB Spore germination protein, GRKA Length = 533 Score = 389 bits (998), Expect = e-106, Method: Compositional matrix adjust. Identities = 188/457 (41%), Positives = 297/457 (64%), Gaps = 1/457 (0%) Query: 31 LRCCLIYVEGMIDRTLLQNGITKPVMAYP-FKEEDCGNSAELIEKLRAEIIALSDITVTT 89 ++ LI+ +G+ ++ ++ I KP M K + S+ +++ ++ +I++ D+ + Sbjct: 66 IKAALIFFKGLTEKKIINQTIMKPFMYDDRIKSLEPDLSSGILKVVKDGLISVGDVKESD 125 Query: 90 GLDEVVGGIVGGNTLLLLDGYAGALSISTQGLETRAIEEPTTEKAVRGPREGFTESLFVN 149 ++ +V + GNT+ LLDG AL + TQG +RAIEEP T+ +RGPREGF+E+L N Sbjct: 126 DINNLVSTCLSGNTIFLLDGSNKALIVDTQGWPSRAIEEPKTDVVIRGPREGFSENLNSN 185 Query: 150 LTLIRRKVQNPDLKFVFRDIGSRTKTQTCICYMEGLASPEILAELQGRLDRVEIDHVLDT 209 ++L+RRK++NP+L F IG +T T C+ Y++ + +P+++ E++ RLD+++ D +L++ Sbjct: 186 MSLLRRKIKNPNLTFENMKIGKQTDTDICLVYLKNVVNPKLIEEIRTRLDKIDTDSILES 245 Query: 210 GYLAELIRDEPYSPFEMLGSTERPDAVVGKIMEGRIALLIEGSPFALTLPYVFVENFQAA 269 GY+ + I DE +S F +G++E+PD V KI+EGR A+L++G+PFALT+P +F+E FQ++ Sbjct: 246 GYIEQYIEDESFSIFPTIGNSEKPDIVAAKILEGRAAILVDGTPFALTVPMLFIEGFQSS 305 Query: 270 EDYYINYYFASFNRFLRVMGAFMSISIPAAYVALVTYSQEMVPTLLLLSIATARLSVPFP 329 EDYY Y+ASF R LR + +SI +P YVAL T+ QE++PT LL ++A A VPFP Sbjct: 306 EDYYSRPYYASFIRILRFVSFSISILLPVTYVALSTFHQELIPTTLLFTMAAAHEGVPFP 365 Query: 330 TVVEALIMLTIFEILREAGARIPTSIGQAVSIVGALVLGQAAVDARIVSAPMVIVVGLTG 389 +EAL+M+ +FEILREAG R+P ++GQAVSIVGALV+G+A V A +V MVIVV LT Sbjct: 366 AFLEALMMMIVFEILREAGIRLPRNVGQAVSIVGALVIGEAVVSAGLVGGIMVIVVALTA 425 Query: 390 ITTLLNPRLTGPMIIVRXXXXXXXXXXXXXXXXXXXXXXVIHLMSLRSFGVPYMLGVGSI 449 I + + P T I+R +IHL+SLRSFG PY+ + + Sbjct: 426 IASFVVPSYTDVTAILRLLLLFLGALLGIYGIGIGLLAVLIHLVSLRSFGTPYLSPLAPV 485 Query: 450 RPQDIKDTAIRAPWWDMYLRPAVMGARNMKRKLPGKR 486 +D+KD+ +R P W M+ RP +G N+++ G + Sbjct: 486 TARDLKDSFLRFPLWSMFTRPRTIGWHNLRKSNSGNK 522 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.140 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,607,967,454 Number of extensions: 103102416 Number of successful extensions: 276974 Number of sequences better than 1.0e-05: 1083 Number of HSP's gapped: 275246 Number of HSP's successfully gapped: 1084 Length of query: 490 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 350 Effective length of database: 1,741,754,103 Effective search space: 609613936050 Effective search space used: 609613936050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)