BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0010.p (558 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2K8N7.1 C2K8N7_STAAU Cardiolipin synthetase 111 2e-22 C2GCF9.1 C2GCF9_STAAU Cardiolipin synthetase 111 2e-22 Q6GH88.1 CLS1_STAAR Cardiolipin synthetase 1 EC=2.7.8.- 111 2e-22 Q6G9P0.1 Q6G9P0_STAAS Putative cardiolipin synthase 109 7e-22 B9M6Q1.1 B9M6Q1_GEOSF Phospholipase D/Transphosphatidylase 106 5e-21 >C2K8N7.1 C2K8N7_STAAU Cardiolipin synthetase Length = 493 Score = 111 bits (277), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 107/415 (25%), Positives = 170/415 (40%), Gaps = 74/415 (17%) Query: 124 TRNNRVDAFVNGPQCLDMLLEEIGKARRYIHLSIMLFFNDGSGNRIADALLRALERGVQV 183 T NN+VD F++G D +L++I A+ YIHL F DG G RI AL L++G++V Sbjct: 127 TENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEV 186 Query: 184 RIMVNYTVTALGYGHNLEVGKFSEISDQLKKAGAKLQDTFNSYYTAAEWHRRRAELKAQG 243 +I+ + + N+++ F D K G +++ F S + Sbjct: 187 KILYDDVGSK-----NVKMANF----DHFKSLGGEVEAFFASKLPLLNFR---------- 227 Query: 244 VPESILFLQDKVQEDVELTGLNVIDHRKFMIIDGITSIIGSLNFGDQYTFAGPIEPSASV 303 +N +HRK +IIDG +G N GD+Y G + Sbjct: 228 --------------------MNNRNHRKIIIIDGQLGYVGGFNIGDEYLGLGKLG----- 262 Query: 304 QVDGRPMGVPAREEEWHDGCFRIRGAAALPLNAVFRSRWLLLGGDPFDPEDAFYRPEGNT 363 W D RI+G A L F W P D Y P+ N Sbjct: 263 --------------YWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNG 308 Query: 364 DLGSEECTLFVSFPGNPVNLIQQYYLDLITYAAEETVIVNPYLI-DQAFWDRLGGVXXXG 422 LG+ + S P + + I+ Y +I A + + +PY I D ++ + + G Sbjct: 309 PLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSG 368 Query: 423 GLGPECSCHLTI-CNPLEVNDHPTNRAAVRSNMYNPFCNGVSFYDYSATGRFSHWKITY- 480 HL I C P DHP A SN + +GV Y Y F H K+ Sbjct: 369 -----VDVHLMIPCKP----DHPLVYWATFSNASDLLSSGVKIYTYE--NGFIHSKMCLI 417 Query: 481 DHRSRAVFHGSYNINERSACHDFELGLLVKGEAFAAKVKTMIDYDLSVSRKITDK 535 D +V G+ N++ RS +FE+ V E A ++ ++D++ S+++T++ Sbjct: 418 DDEIVSV--GTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTEE 470 >C2GCF9.1 C2GCF9_STAAU Cardiolipin synthetase Length = 493 Score = 111 bits (277), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 107/415 (25%), Positives = 170/415 (40%), Gaps = 74/415 (17%) Query: 124 TRNNRVDAFVNGPQCLDMLLEEIGKARRYIHLSIMLFFNDGSGNRIADALLRALERGVQV 183 T NN+VD F++G D +L++I A+ YIHL F DG G RI AL L++G++V Sbjct: 127 TENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEV 186 Query: 184 RIMVNYTVTALGYGHNLEVGKFSEISDQLKKAGAKLQDTFNSYYTAAEWHRRRAELKAQG 243 +I+ + + N+++ F D K G +++ F S + Sbjct: 187 KILYDDVGSK-----NVKMANF----DHFKSLGGEVEAFFASKLPLLNFR---------- 227 Query: 244 VPESILFLQDKVQEDVELTGLNVIDHRKFMIIDGITSIIGSLNFGDQYTFAGPIEPSASV 303 +N +HRK +IIDG +G N GD+Y G + Sbjct: 228 --------------------MNNRNHRKIIIIDGQLGYVGGFNIGDEYLGLGKLG----- 262 Query: 304 QVDGRPMGVPAREEEWHDGCFRIRGAAALPLNAVFRSRWLLLGGDPFDPEDAFYRPEGNT 363 W D RI+G A L F W P D Y P+ N Sbjct: 263 --------------YWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNG 308 Query: 364 DLGSEECTLFVSFPGNPVNLIQQYYLDLITYAAEETVIVNPYLI-DQAFWDRLGGVXXXG 422 LG+ + S P + + I+ Y +I A + + +PY I D ++ + + G Sbjct: 309 PLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSG 368 Query: 423 GLGPECSCHLTI-CNPLEVNDHPTNRAAVRSNMYNPFCNGVSFYDYSATGRFSHWKITY- 480 HL I C P DHP A SN + +GV Y Y F H K+ Sbjct: 369 -----VDVHLMIPCKP----DHPLVYWATFSNASDLLSSGVKIYTYE--NGFIHSKMCLI 417 Query: 481 DHRSRAVFHGSYNINERSACHDFELGLLVKGEAFAAKVKTMIDYDLSVSRKITDK 535 D +V G+ N++ RS +FE+ V E A ++ ++D++ S+++T++ Sbjct: 418 DDEIVSV--GTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTEE 470 >Q6GH88.1 CLS1_STAAR Cardiolipin synthetase 1 EC=2.7.8.- Length = 493 Score = 111 bits (277), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 107/415 (25%), Positives = 170/415 (40%), Gaps = 74/415 (17%) Query: 124 TRNNRVDAFVNGPQCLDMLLEEIGKARRYIHLSIMLFFNDGSGNRIADALLRALERGVQV 183 T NN+VD F++G D +L++I A+ YIHL F DG G RI AL L++G++V Sbjct: 127 TENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEV 186 Query: 184 RIMVNYTVTALGYGHNLEVGKFSEISDQLKKAGAKLQDTFNSYYTAAEWHRRRAELKAQG 243 +I+ + + N+++ F D K G +++ F S + Sbjct: 187 KILYDDVGSK-----NVKMANF----DHFKSLGGEVEAFFASKLPLLNFR---------- 227 Query: 244 VPESILFLQDKVQEDVELTGLNVIDHRKFMIIDGITSIIGSLNFGDQYTFAGPIEPSASV 303 +N +HRK +IIDG +G N GD+Y G + Sbjct: 228 --------------------MNNRNHRKIIIIDGQLGYVGGFNIGDEYLGLGKLG----- 262 Query: 304 QVDGRPMGVPAREEEWHDGCFRIRGAAALPLNAVFRSRWLLLGGDPFDPEDAFYRPEGNT 363 W D RI+G A L F W P D Y P+ N Sbjct: 263 --------------YWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNG 308 Query: 364 DLGSEECTLFVSFPGNPVNLIQQYYLDLITYAAEETVIVNPYLI-DQAFWDRLGGVXXXG 422 LG+ + S P + + I+ Y +I A + + +PY I D ++ + + G Sbjct: 309 PLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSG 368 Query: 423 GLGPECSCHLTI-CNPLEVNDHPTNRAAVRSNMYNPFCNGVSFYDYSATGRFSHWKITY- 480 HL I C P DHP A SN + +GV Y Y F H K+ Sbjct: 369 -----VDVHLMIPCKP----DHPLVYWATFSNASDLLSSGVKIYTYE--NGFIHSKMCLI 417 Query: 481 DHRSRAVFHGSYNINERSACHDFELGLLVKGEAFAAKVKTMIDYDLSVSRKITDK 535 D +V G+ N++ RS +FE+ V E A ++ ++D++ S+++T++ Sbjct: 418 DDEIVSV--GTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTEE 470 >Q6G9P0.1 Q6G9P0_STAAS Putative cardiolipin synthase Length = 493 Score = 109 bits (273), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 105/413 (25%), Positives = 167/413 (40%), Gaps = 74/413 (17%) Query: 124 TRNNRVDAFVNGPQCLDMLLEEIGKARRYIHLSIMLFFNDGSGNRIADALLRALERGVQV 183 T NN+VD F++G D +L++I A+ YIHL F DG G RI AL L++G++V Sbjct: 127 TENNKVDHFIDGNDLYDQVLQDIKNAKEYIHLEYYTFALDGLGQRILKALEEKLKQGLEV 186 Query: 184 RIMVNYTVTALGYGHNLEVGKFSEISDQLKKAGAKLQDTFNSYYTAAEWHRRRAELKAQG 243 +I+ + + +++ F D K G +++ F S + Sbjct: 187 KILYDDVGSK-----KVKMANF----DHFKSLGGEVEAFFASKLPLLNFR---------- 227 Query: 244 VPESILFLQDKVQEDVELTGLNVIDHRKFMIIDGITSIIGSLNFGDQYTFAGPIEPSASV 303 +N +HRK ++IDG +G N GD+Y G + Sbjct: 228 --------------------MNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLG----- 262 Query: 304 QVDGRPMGVPAREEEWHDGCFRIRGAAALPLNAVFRSRWLLLGGDPFDPEDAFYRPEGNT 363 W D RI+G A L F W P D Y P+ N Sbjct: 263 --------------YWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNG 308 Query: 364 DLGSEECTLFVSFPGNPVNLIQQYYLDLITYAAEETVIVNPYLI-DQAFWDRLGGVXXXG 422 LG+ + S P + + I+ Y +I A + + +PY I D ++ + + G Sbjct: 309 PLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSG 368 Query: 423 GLGPECSCHLTI-CNPLEVNDHPTNRAAVRSNMYNPFCNGVSFYDYSATGRFSHWKITY- 480 HL I C P DHP A SN + +GV Y Y F H K+ Sbjct: 369 -----VDVHLMIPCKP----DHPLVYWATYSNASDLLSSGVKIYTYE--NGFIHSKMCLI 417 Query: 481 DHRSRAVFHGSYNINERSACHDFELGLLVKGEAFAAKVKTMIDYDLSVSRKIT 533 D +V G+ N++ RS +FE+ V E A ++ ++D++ S+++T Sbjct: 418 DDEMVSV--GTANMDFRSFELNFEVNAFVYDEKLAKDLRVAYEHDITKSKQLT 468 >B9M6Q1.1 B9M6Q1_GEOSF Phospholipase D/Transphosphatidylase Length = 395 Score = 106 bits (265), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 108/421 (25%), Positives = 167/421 (39%), Gaps = 90/421 (21%) Query: 123 TTRNNRVDAFVNGPQCLDMLLEEIGKARRYIHLSIMLFFNDGSGNRIADALLRALERGVQ 182 +R NRV+ F NG + L + IG+A + I L L +D +G +ADA+L+A ERGV+ Sbjct: 30 ASRGNRVELFRNGEEFFSALFQAIGQAEKVICLEFYLIRDDNTGRALADAVLQAAERGVK 89 Query: 183 VRIMVNYTVTALGYGHNLEVGKFSEISDQLKK--AGAKLQDTFN--SYYTAAEWHRRRAE 238 V ++ +Y +G F ++ G + FN ++ W +R Sbjct: 90 VYLLYDY------------IGCFDTPGAYFRRLEKGGIVCCPFNPPTFSKGIGWFDKR-- 135 Query: 239 LKAQGVPESILFLQDKVQEDVELTGLNVIDHRKFMIIDGITSIIGSLNFGDQYTFAGPIE 298 DHRK +IDG T+ G LN GD+Y Sbjct: 136 -----------------------------DHRKIAVIDGRTAFTGGLNIGDEYA------ 160 Query: 299 PSASVQVDGRPMGVPAREEEWHDGCFRIRGAAALPLNAVFRSRWLLLGGDPFDPEDAFYR 358 G + W D R+ G A LN +FR W + Sbjct: 161 ------------GCGEDFDRWRDVGIRLEGPAVPELNRLFRENW---------HRERSTI 199 Query: 359 PEGNTD------LGSEECTLFVSFPGNPVNLIQQYYLDLITYAAEETVIVNPYLIDQAFW 412 PEG D G +E + P + + I+ + I A+E VI NPY + Sbjct: 200 PEGCVDPYLLDSAGEDEVLIVNGGPHHNRSFIRSAFRMAIAGASESIVIANPYFVPGPRV 259 Query: 413 DRLGGVXXXGGLGPECSCHLTICNPLEVNDHPTNRAAVRSNMYNPFCNGVSFYDYSATGR 472 R + G G + L P + +D P R RS + NG+ Y+ T Sbjct: 260 IR--SLLRAVGRGVQVRLIL----PAK-SDVPIVRLVSRSYYASLLKNGIEIYERQGT-- 310 Query: 473 FSHWKITYDHRSRAVFHGSYNINERSACHDFELGLLVKGEAFAAKVKTMIDYDLSVSRKI 532 H K+ +V GS N+++RS ++E+ ++V +F A+V M+ DL+ SR+I Sbjct: 311 ILHAKVMIIDSCWSVI-GSANLDQRSFHRNYEVNVIVDSPSFGAQVSQMLMADLARSRRI 369 Query: 533 T 533 Sbjct: 370 V 370 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,462,940,882 Number of extensions: 152063804 Number of successful extensions: 383705 Number of sequences better than 1.0e-05: 187 Number of HSP's gapped: 383948 Number of HSP's successfully gapped: 204 Length of query: 558 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 417 Effective length of database: 1,732,333,088 Effective search space: 722382897696 Effective search space used: 722382897696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)