BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0003.p (259 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D7U7.1 C5D7U7_9BACI Nitrilase/cyanide hydratase and apolipopro... 350 6e-95 B4BI28.1 B4BI28_9BACI Nitrilase/cyanide hydratase and apolipopro... 345 2e-93 A4ILL3.1 A4ILL3_GEOTN Nitrilase 345 2e-93 Q5L1E4.1 Q5L1E4_GEOKA Hypothetical conserved protein 343 6e-93 B7GLF6.1 B7GLF6_ANOFW Predicted amidohydrolase 343 1e-92 >C5D7U7.1 C5D7U7_9BACI Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase Length = 271 Score = 350 bits (898), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 161/258 (62%), Positives = 191/258 (74%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEG 60 +KI+ LQLDIAFGNP N D ++LPELWTTGYDLTRL EIAD G Sbjct: 3 IKIACLQLDIAFGNPNENERRVEREIEKISKNHPDIIVLPELWTTGYDLTRLDEIADEGG 62 Query: 61 NATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEH 120 TKA + LA+ ++IVAGS+A + D G+ N+MYI R G + GEYSKLHLF+LMDEH Sbjct: 63 MRTKAFIQKLAKSHHVNIVAGSIARKTDRGVTNTMYIADRNGIIVGEYSKLHLFQLMDEH 122 Query: 121 LYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRA 180 LYLQPG GLFTL+ CAG+ICYDIRFPEW+R H GAE+LF+ AEWPLPRL+HWR Sbjct: 123 LYLQPGEEMGLFTLENTRCAGVICYDIRFPEWIRVHMVQGAEVLFVVAEWPLPRLSHWRT 182 Query: 181 LLISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKV 240 LL +RAIENQCYVIACNRAG DP+N FAGHS+++DPWGE+I EA+ L +IDLQKV Sbjct: 183 LLAARAIENQCYVIACNRAGSDPNNVFAGHSLVVDPWGEIIAEANEEPCTLMAQIDLQKV 242 Query: 241 REVRAQIPVFSDRRPELY 258 REVR QIP+F+DRR Y Sbjct: 243 REVRKQIPIFADRRANDY 260 >B4BI28.1 B4BI28_9BACI Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase Length = 271 Score = 345 bits (886), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 157/259 (60%), Positives = 194/259 (74%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEG 60 ++I+ LQLDIAFG+PQ N +G D ++LPELWTTGYDLTRL EIAD Sbjct: 3 IRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLGEIADDNA 62 Query: 61 NATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEH 120 K S LA+ GIH VAGSVA + D+G+ N+M + +R G++ EYSK+HLF+LMDEH Sbjct: 63 ERAKTFASRLAQTYGIHFVAGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMDEH 122 Query: 121 LYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRA 180 L+LQ G GLF+LD SCAG+ICYDIRFPEW+R H SGAE+LF+ AEWPLPRL HWR Sbjct: 123 LHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHALSGAELLFVVAEWPLPRLHHWRT 182 Query: 181 LLISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKV 240 LL++RAIENQCYV+ACNRAG DP+N FAGHS+IIDPWGE++ EA IL +ID V Sbjct: 183 LLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPALV 242 Query: 241 REVRAQIPVFSDRRPELYQ 259 R+VR++IPVF+DRRP LY+ Sbjct: 243 RDVRSRIPVFADRRPRLYE 261 >A4ILL3.1 A4ILL3_GEOTN Nitrilase Length = 271 Score = 345 bits (885), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 157/259 (60%), Positives = 194/259 (74%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEG 60 ++I+ LQLDIAFG+PQ N +G D ++LPELWTTGYDLTRL EIAD Sbjct: 3 IRIACLQLDIAFGDPQKNEQQAEKAVELAVKEGADIIVLPELWTTGYDLTRLDEIADDNA 62 Query: 61 NATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEH 120 K S LA+ GIH VAGSVA + D+G+ N+M + +R G++ EYSK+HLF+LMDEH Sbjct: 63 ERAKTFASRLAQTYGIHFVAGSVAKKTDAGVTNTMIVANRNGQIIKEYSKVHLFQLMDEH 122 Query: 121 LYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRA 180 L+LQ G GLF+LD SCAG+ICYDIRFPEW+R H SGAE+LF+ AEWPLPRL HWR Sbjct: 123 LHLQSGNEPGLFSLDELSCAGVICYDIRFPEWIRTHALSGAELLFVVAEWPLPRLHHWRT 182 Query: 181 LLISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKV 240 LL++RAIENQCYV+ACNRAG DP+N FAGHS+IIDPWGE++ EA IL +ID V Sbjct: 183 LLMARAIENQCYVVACNRAGSDPNNVFAGHSLIIDPWGEILAEADEDPGILLADIDPALV 242 Query: 241 REVRAQIPVFSDRRPELYQ 259 R+VR++IPVF+DRRP LY+ Sbjct: 243 RDVRSRIPVFADRRPRLYE 261 >Q5L1E4.1 Q5L1E4_GEOKA Hypothetical conserved protein Length = 270 Score = 343 bits (881), Expect = 6e-93, Method: Compositional matrix adjust. Identities = 157/259 (60%), Positives = 195/259 (75%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEG 60 ++I+ LQLDIAFG+P N G + ++LPELWTTGYDLTRL EIAD Sbjct: 3 IRIACLQLDIAFGDPNENERCAESAIESAVKDGANIIVLPELWTTGYDLTRLDEIADEGA 62 Query: 61 NATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEH 120 KA S LA+ IH+VAGSVA + +G+ N+M + R+G++ GEYSKLHLF+LMDEH Sbjct: 63 ERAKAFASRLAQAHNIHLVAGSVAKKTTTGVTNTMIVADRSGQIVGEYSKLHLFQLMDEH 122 Query: 121 LYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRA 180 LYLQPG++ GLF+LDG +CAG+ICYDIRFPEW+RAH +GAE+LF+ AEWPLPRL HWR Sbjct: 123 LYLQPGSSLGLFSLDGLACAGVICYDIRFPEWIRAHALAGAEVLFVVAEWPLPRLHHWRT 182 Query: 181 LLISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKV 240 LL++RAIENQCYV+ACNRAG DP+N FAGHS++IDPWGEVI EA IL +ID V Sbjct: 183 LLMARAIENQCYVVACNRAGRDPNNVFAGHSLVIDPWGEVIAEADEKPGILSADIDPALV 242 Query: 241 REVRAQIPVFSDRRPELYQ 259 +VR +IPVF+DRRP Y+ Sbjct: 243 ADVRTRIPVFADRRPHDYE 261 >B7GLF6.1 B7GLF6_ANOFW Predicted amidohydrolase Length = 267 Score = 343 bits (879), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 153/259 (59%), Positives = 195/259 (75%), Gaps = 1/259 (0%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEG 60 M I+ +Q+DIAFG P+ N + D V+LPELWTTGYDLTRL +IAD G Sbjct: 4 MNIACVQMDIAFGAPEKNKQTVVRYMEQIAKEA-DVVVLPELWTTGYDLTRLTDIADENG 62 Query: 61 NATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEH 120 TKA +S LAR +HIV GSVA + I N+MYI R G++ EYSKLHLF+LMDEH Sbjct: 63 AETKAFLSELARTYDVHIVGGSVAKKVADNITNTMYIVHRNGDVVCEYSKLHLFKLMDEH 122 Query: 121 LYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRA 180 LYLQ G GL +LD +CAG+ICYDIRFPEW+RAHT +GAE+LF+ AEWPL RL HWR Sbjct: 123 LYLQAGETTGLCSLDETTCAGVICYDIRFPEWIRAHTLNGAEVLFVVAEWPLSRLHHWRT 182 Query: 181 LLISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKV 240 LL++RAIENQCYV+ACNRAG+DP+NTFAGHSM+IDPWG ++ E + E +++ ID+ +V Sbjct: 183 LLMARAIENQCYVVACNRAGKDPNNTFAGHSMMIDPWGNILAEGNEQETVIKATIDINEV 242 Query: 241 REVRAQIPVFSDRRPELYQ 259 + VRA+IP+F+DRRP++Y+ Sbjct: 243 QRVRARIPIFADRRPDVYK 261 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,481,843,932 Number of extensions: 58940393 Number of successful extensions: 158488 Number of sequences better than 1.0e-05: 2708 Number of HSP's gapped: 152748 Number of HSP's successfully gapped: 2709 Length of query: 259 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 126 Effective length of database: 1,807,701,208 Effective search space: 227770352208 Effective search space used: 227770352208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)