BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0234.0002.p (354 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1Y5M6.1 B1Y5M6_LEPCP Cobalamin B12-binding domain protein 277 1e-72 C1SVK5.1 C1SVK5_9DELT Predicted cobalamin binding protein 241 1e-61 O26227.1 O26227_METTH Conserved protein 228 6e-58 A6CFX0.1 A6CFX0_9PLAN Sensor protein EC=2.7.13.3 226 4e-57 A9EQU7.1 A9EQU7_SORC5 Putative cobalamin binding protein 218 5e-55 >B1Y5M6.1 B1Y5M6_LEPCP Cobalamin B12-binding domain protein Length = 398 Score = 277 bits (708), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 142/355 (40%), Positives = 213/355 (60%), Gaps = 14/355 (3%) Query: 7 NAGERLKALAEQLAEEVTLRQYERQPELRQRFGPSGVARTKQDSLYHFRYLAQSVALDSP 66 +AG +L+ A LA+ V + ++ R P LR R+G +G A+ ++D+ YHF YLA +V SP Sbjct: 47 SAGHQLRDQAGALADAVVVCEFARYPRLRARYGAAGRAKARRDAGYHFAYLADAVDAGSP 106 Query: 67 LLFINYIIWLKVLLAQYKITAEDLQINLDLMKEAISSQVETPEKELILSYLDMGLHHTRG 126 LF +YI W+KVLLAQ + +EDL +L M + + Q+ P + ++ +D Sbjct: 107 ALFNDYIGWVKVLLAQRNVRSEDLDHHLACMADVLREQMPPPVADPAIAMIDGA------ 160 Query: 127 EESLPT-------FLQPEKPYYREAEEYLKLLLAGERRKASEFVLGLHEGGVPIRDLYLH 179 +LPT F+ P + A EY+ LL G R+ A V E G +RDLYL Sbjct: 161 RAALPTLPSTSASFIDPGERLAPLAREYVHTLLGGYRKAAGRLVFDAAERGESVRDLYLQ 220 Query: 180 LFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYPRWIQAAQTGGKTLVAVGVGEE 239 +FQ + E+G LWQ+ +I+VAQEH+C+A TQ ++SQL PR I + GG +V V + + Sbjct: 221 VFQPALREVGLLWQMKKISVAQEHFCSAATQVVMSQLLPRAITDTR-GGHRVVVVCISGD 279 Query: 240 LHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSVTMAYHVSEVQRL 299 LHE+G RM+ DFFEM GW+ Y G++ P + +++ L E+ AD+LAVS TM H+ V+ L Sbjct: 280 LHEVGARMVGDFFEMAGWDAYYCGASTPHDAVVQALAERAADVLAVSATMGCHLHGVKEL 339 Query: 300 IASIRTHAGLDRIKILVGGMPFNIDRTLWEKVGADGYAPDAKGALEVASQLISQN 354 I +R R++++VGG PF +D L VGADG A DA+ A+ +A ++Q+ Sbjct: 340 IERVRADPRCARVRVMVGGPPFTVDPALCRAVGADGTASDAEAAVALAGHWLAQS 394 >C1SVK5.1 C1SVK5_9DELT Predicted cobalamin binding protein Length = 349 Score = 241 bits (614), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 124/301 (41%), Positives = 191/301 (63%), Gaps = 3/301 (0%) Query: 53 HFRYLAQSVALDSPLLFINYIIWLKVLLAQYKITAEDLQINLDLMKEAISSQVETPEKEL 112 H YL+++V LF Y+ W + L A+ + L+ L +++ + + + Sbjct: 50 HLLYLSEAVRSGEVELFTEYMHWAQSLEAEGGYSQHYLETVLQCLQKVLGYHLPESQARF 109 Query: 113 ILSYLDMGLHHTRGEESLP-TFLQPEKPYYREAEEYLKLLLAGERRKASEFVLGLHEGGV 171 + +LD + + + P +++ + P +A+ YL LLAG+R A + + + GG Sbjct: 110 VDPFLDAAVAALYDDTTPPASYISAKSPLAEQAQTYLDALLAGKRHDALQLIQTMARGGA 169 Query: 172 PIRDLYLHLFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYPRWIQAAQTGGKTL 231 P++D+YL +FQ+ QYEIGRLWQL +I+VAQEH+CTA TQ ++QLYP +I A + G+TL Sbjct: 170 PVQDIYLDVFQSVQYEIGRLWQLNQISVAQEHFCTAVTQMAMAQLYP-YIFATRRVGRTL 228 Query: 232 VAVGVGEELHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSVTMAY 291 VA VG ELHE+G RM+ADFFEM GW+T YLG+N P+ +I+ + +Q AD++ +SVTM Y Sbjct: 229 VASCVGGELHELGPRMVADFFEMAGWDTYYLGANTPSASVIQTVLDQRADVVGISVTMTY 288 Query: 292 HVSEVQRLIASIRTHAGLDRIKILVGGMPFNIDRTLWEKVGADGYAPDAKGALEVASQLI 351 +V + Q LIA++R G D ++ILVGG PF I TL +GADG A +A+ A+ +A L+ Sbjct: 289 YVHKAQDLIAALRDELG-DSVRILVGGYPFVISTTLARDIGADGSAQNAQDAVRLAEHLV 347 Query: 352 S 352 + Sbjct: 348 A 348 >O26227.1 O26227_METTH Conserved protein Length = 314 Score = 228 bits (581), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 122/304 (40%), Positives = 181/304 (59%), Gaps = 3/304 (0%) Query: 48 QDSLYHFRYLAQSVALDSPLLFINYIIWLKVLLAQYKITAEDLQINLDLMKEAISSQVET 107 QD YH YL +++ SP LF +Y++W VLL + E L+ ++ ++ AI + Sbjct: 12 QDINYHLDYLEEAILNSSPELFSDYVLWADVLLKNLGLPEECLRGSIKSIERAIMKLADD 71 Query: 108 PEKELILSYLDMGLHHTRGEESLPTFLQPEKPYYREAEEYLKLLLAGERRKASEFVLGLH 167 + Y+ L + L ++P AEEYL+L+L E R+A E ++ Sbjct: 72 EVADKASEYISGALKILETSHPSSSHLG-DQPLRELAEEYLRLVLNTEAREARELIVSSL 130 Query: 168 EGGVPIRDLYLHLFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYPRWIQAAQTG 227 E G+ + D+YL +F+ Q+EIGRLWQ +I+VA EHY T+ TQSI+S+LYP A++ Sbjct: 131 ESGIRVEDIYLQVFEPVQHEIGRLWQTNQISVAHEHYATSVTQSIMSELYPYIYSASERR 190 Query: 228 GKTLVAVGVGEELHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSV 287 LVA V ELHEIG+RM++DFFEM GW + YLG+N P E + + DL+A+S Sbjct: 191 NLKLVAACVNNELHEIGIRMVSDFFEMNGWESIYLGANTPPEDFSGIVSDLKPDLVALSA 250 Query: 288 TMAYHVSEVQRLIASIRTHAGLDRIKILVGGMPFNIDRTLWEKVGADGYAPDAKGALEVA 347 TM ++VS V+ +I + DR ++VGG PFN+D LW+KVGAD +A DA GA++VA Sbjct: 251 TMTFNVSHVRHIIELLDELE--DRPPVMVGGYPFNVDIKLWKKVGADLHAADASGAVKVA 308 Query: 348 SQLI 351 + + Sbjct: 309 EEFL 312 >A6CFX0.1 A6CFX0_9PLAN Sensor protein EC=2.7.13.3 Length = 760 Score = 226 bits (575), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 121/313 (38%), Positives = 179/313 (57%), Gaps = 2/313 (0%) Query: 39 GPSGVARTKQDSLYHFRYLAQSVALDSPLLFINYIIWLKVLLAQYKITAEDLQINLDLMK 98 G V R +Q S H LA S+ S +L +Y+ W+ L ++ LD + Sbjct: 4 GTPPVTRLEQ-SETHILILAASIEFKSTVLLNDYVHWVTRLPESVSPYFCEISHFLDALI 62 Query: 99 EAISSQVETPEKELILSYLDMGLHHTRGEESLPTFLQPEKPYYREAEEYLKLLLAGERRK 158 + ++ ++ Y+D + + + + P + YL LLA R Sbjct: 63 SVVEAEFPQEVANVVQHYIDTARNSLLVDRPVEPPARVCPPLAQLQHNYLTSLLATHRTD 122 Query: 159 ASEFVLGLHEGGVPIRDLYLHLFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYP 218 A V+ + GV I +YL + Q +Q+E+GRLWQ G+I+VAQEHYCTA TQ ++SQL P Sbjct: 123 ALNMVMKAIQSGVEIESIYLDVIQPAQHELGRLWQTGQISVAQEHYCTAATQFVMSQLQP 182 Query: 219 RWIQAAQTGGKTLVAVGVGEELHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQ 278 W + +TLVA VG+ELHE+GLR++AD FE+ GWNT YLG+N+P E + L Sbjct: 183 -WFLKTTSADQTLVATCVGDELHEVGLRIVADLFEVSGWNTIYLGANVPPESIAETLASS 241 Query: 279 PADLLAVSVTMAYHVSEVQRLIASIRTHAGLDRIKILVGGMPFNIDRTLWEKVGADGYAP 338 A +LA+S TM H+ + +I +R+H+G +++KIL GG PFNID LW++VGAD A Sbjct: 242 RAQILAISCTMTQHLFAMADVIRIVRSHSGCEQVKILAGGYPFNIDPYLWKRVGADAAAL 301 Query: 339 DAKGALEVASQLI 351 D K A++VA++LI Sbjct: 302 DGKDAIDVANRLI 314 >A9EQU7.1 A9EQU7_SORC5 Putative cobalamin binding protein Length = 502 Score = 218 bits (556), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 120/311 (38%), Positives = 183/311 (58%), Gaps = 14/311 (4%) Query: 49 DSLYHFRYLAQSVALDSPLLFINYIIWLKVLLAQYKITAEDLQINL----DLMKEAISSQ 104 D+ H LA ++ SP LF Y W + L Q+ + +L L D++ + + Sbjct: 195 DARRHVAQLAAALDNGSPELFTAYAEWAQTRLVQHGLDPAELAGYLRALGDVLALTLPER 254 Query: 105 VETPEKELILSYLDMGLHHTRGEESLPTFLQPEKPYYREAEEYLKLLLAGERRKASEFVL 164 + + + + LD L T E+ P+F++PE P A YL L+ +RR+A + Sbjct: 255 LAEAPRRYLAAALDR-LTSTHAED--PSFVRPEAPLGAVACTYLDALIERDRRRAVRVIR 311 Query: 165 GLHEGGVPIRDLYLHLFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYPRWIQAA 224 + G PIRD+Y H+ + QYE+GR WQL ++++A EHYCTA TQ I+ QLYP + +A Sbjct: 312 EALDAGAPIRDIYAHVLEPCQYEVGRRWQLRQLSIAGEHYCTAVTQMILGQLYP-LVVSA 370 Query: 225 QTGGKTLVAVGVGEELHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLA 284 G+ LVA V LHEIG R +ADFFEM GW+T YLG++ PAE +I+ + ++ AD+LA Sbjct: 371 DRAGRRLVATAVDGNLHEIGARFVADFFEMAGWDTFYLGASTPAEHVIQEVVQRKADVLA 430 Query: 285 VSVTMAYHVSEVQRLIASIRTHAGLDRIK---ILVGGMPFNIDRTLWEKVGADGYAPDAK 341 VS ++ +S V+++I ++R G DR + +L GG PF + LW ++GADG AP A+ Sbjct: 431 VSAALSDQLSSVRQVITAVR---GDDRCRGVVVLAGGQPFRVVDDLWRQLGADGSAPSAE 487 Query: 342 GALEVASQLIS 352 A+ A QL++ Sbjct: 488 RAVRTAEQLLA 498 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,075,479,549 Number of extensions: 85267947 Number of successful extensions: 250229 Number of sequences better than 1.0e-05: 259 Number of HSP's gapped: 250237 Number of HSP's successfully gapped: 259 Length of query: 354 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 217 Effective length of database: 1,770,017,148 Effective search space: 384093721116 Effective search space used: 384093721116 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)