BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0023.p (270 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4ILF6.1 A4ILF6_GEOTN 2,5-diketo-D-gluconic acid reductase 439 e-121 C0ZJH8.1 C0ZJH8_BREBN Probable 2,5-diketo-D-gluconic acid reduct... 429 e-118 O34678.1 YTBE_BACSU Uncharacterized oxidoreductase ytbE EC=1.-.-.- 413 e-114 B7JEM0.1 B7JEM0_BACC0 Oxidoreductase, aldo/keto reductase family 407 e-112 C3GRA5.1 C3GRA5_BACTU YtbE (Aldo/keto reductase YtbE) 407 e-112 >A4ILF6.1 A4ILF6_GEOTN 2,5-diketo-D-gluconic acid reductase Length = 281 Score = 439 bits (1129), Expect = e-121, Method: Compositional matrix adjust. Identities = 199/270 (73%), Positives = 239/270 (88%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L++GV MPWFG+GVF+VEEG EL+ AVK+AI +GYRS+DTAAIY NE GVGQ + E + E Sbjct: 12 LHNGVKMPWFGIGVFKVEEGPELVNAVKTAIKHGYRSIDTAAIYGNEVGVGQGIREGMKE 71 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 ++RE+LF+TSKVWN DLGYE+T+AAY+ SL KLGL+YLDLYLIHWPV+GKYKDAWRAL Sbjct: 72 TGISREELFVTSKVWNTDLGYESTIAAYETSLTKLGLEYLDLYLIHWPVEGKYKDAWRAL 131 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 ETLY GRVKAIGVSNFQIHHLED+++DA+I PMVNQVE HP L+Q+EL+ FC+E GIQ+ Sbjct: 132 ETLYKEGRVKAIGVSNFQIHHLEDLMKDAEIKPMVNQVEYHPRLTQKELQSFCQEHGIQL 191 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 EAWSPLMQGQLL+ VL+EIA + KSVAQVILRWDLQ+G+VTIPK+TKEHRI+ENASVF Sbjct: 192 EAWSPLMQGQLLNNEVLQEIANKYNKSVAQVILRWDLQNGVVTIPKTTKEHRIVENASVF 251 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 DFEL+ DM++IDGLNQ+ R GPDPD+FDF Sbjct: 252 DFELTEEDMKKIDGLNQNHRVGPDPDNFDF 281 >C0ZJH8.1 C0ZJH8_BREBN Probable 2,5-diketo-D-gluconic acid reductase EC=1.1.1.- Length = 280 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 200/270 (74%), Positives = 230/270 (85%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L +GV MPW GLGVF+VEEG EL AVK+AI +GYRS+DTAAIY NE GVG+ + L E Sbjct: 11 LYNGVKMPWMGLGVFKVEEGQELELAVKNAIKHGYRSIDTAAIYNNEEGVGRGIRAGLQE 70 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 + REDLF+TSKVWNADLGYE+TL AY+ SL+KLGL+YLDLYLIHWPV+GK+K+AWRAL Sbjct: 71 AGITREDLFVTSKVWNADLGYESTLQAYETSLKKLGLEYLDLYLIHWPVEGKFKEAWRAL 130 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 ETLY G VKAIGVSNF +HHLE++++DA+I PMVNQVE HP LSQ ELR +CKEQGIQ Sbjct: 131 ETLYKKGLVKAIGVSNFHVHHLEELLKDAEIKPMVNQVEFHPRLSQDELRAYCKEQGIQF 190 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 EAWSPLMQGQLLD PVLK IA HGKS+AQVI+RWDLQ+G+VTIPKSTKEHRI+ENASVF Sbjct: 191 EAWSPLMQGQLLDNPVLKGIAEKHGKSIAQVIIRWDLQNGVVTIPKSTKEHRIVENASVF 250 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 DFELS DME I LNQ+ R GPDPD+FDF Sbjct: 251 DFELSKEDMEMIHSLNQNHRVGPDPDNFDF 280 >O34678.1 YTBE_BACSU Uncharacterized oxidoreductase ytbE EC=1.-.-.- Length = 280 Score = 413 bits (1062), Expect = e-114, Method: Compositional matrix adjust. Identities = 201/270 (74%), Positives = 235/270 (87%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L++GV MPWFGLGVFQVEEG+EL+ AVK+AI +GYRS+DTAAIY NE GVG+ + E + E Sbjct: 11 LHNGVEMPWFGLGVFQVEEGSELVNAVKTAIVHGYRSIDTAAIYGNEAGVGEGIREGIEE 70 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 ++REDLFITSKVWNADLGYE TLAA++ SL KLGLDYLDLYLIHWPV+GKYK+AWRAL Sbjct: 71 AGISREDLFITSKVWNADLGYEETLAAFETSLSKLGLDYLDLYLIHWPVEGKYKEAWRAL 130 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 ETLY GR+KAIGVSNFQIHHLED++ A+I PM+NQVE HP L+Q+EL R+C+ QGIQ+ Sbjct: 131 ETLYKEGRIKAIGVSNFQIHHLEDLMTAAEIKPMINQVEFHPRLTQKELIRYCQNQGIQM 190 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 EAWSPLMQGQLLD PVL +IA + KSVAQ+ILRWDLQHGI+TIPKSTKEHRI ENASVF Sbjct: 191 EAWSPLMQGQLLDHPVLADIAQTYNKSVAQIILRWDLQHGIITIPKSTKEHRIKENASVF 250 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 DFEL+ DM RID LN++ R GPDPD+FDF Sbjct: 251 DFELTQDDMNRIDALNENLRVGPDPDNFDF 280 >B7JEM0.1 B7JEM0_BACC0 Oxidoreductase, aldo/keto reductase family Length = 279 Score = 407 bits (1046), Expect = e-112, Method: Compositional matrix adjust. Identities = 196/270 (72%), Positives = 231/270 (85%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 LN+GV MPWFGLGVF+VEEG EL++AVKSAI GYRS+DTAAIY NE+ VG+ + + Sbjct: 10 LNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEA 69 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 +AREDLFITSKVWNAD GYE T+AAY+ SL+KLGLDYLDLYL+HWP +GKYKD WRAL Sbjct: 70 TGIAREDLFITSKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEGKYKDTWRAL 129 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 ETLY RV+AIGVSNFQIHHL+DV++DA+I PM+NQVE HP L+Q+EL+ FCKEQGIQ+ Sbjct: 130 ETLYKEKRVRAIGVSNFQIHHLQDVMKDAEIKPMINQVEYHPRLTQKELQAFCKEQGIQM 189 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 EAWSPLMQGQLLD L+EIA HGK+ AQVILRWDLQ+G++TIPKSTKEHRII NA VF Sbjct: 190 EAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEHRIIANADVF 249 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 +FEL+ DME+ID LNQ+ R GPDPD+FDF Sbjct: 250 NFELTKEDMEKIDALNQNHRVGPDPDNFDF 279 >C3GRA5.1 C3GRA5_BACTU YtbE (Aldo/keto reductase YtbE) Length = 279 Score = 407 bits (1046), Expect = e-112, Method: Compositional matrix adjust. Identities = 196/270 (72%), Positives = 231/270 (85%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 LN+GV MPWFGLGVF+VEEG EL++AVKSAI GYRS+DTAAIY NE+ VG+ + + Sbjct: 10 LNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEA 69 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 +AREDLFITSKVWNAD GYE T+AAY+ SL+KLGLDYLDLYL+HWP +GKYKD WRAL Sbjct: 70 TGIAREDLFITSKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEGKYKDTWRAL 129 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 ETLY RV+AIGVSNFQIHHL+DV++DA+I PM+NQVE HP L+Q+EL+ FCKEQGIQ+ Sbjct: 130 ETLYKEKRVRAIGVSNFQIHHLQDVMKDAEIKPMINQVEYHPRLTQKELQAFCKEQGIQM 189 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 EAWSPLMQGQLLD L+EIA HGK+ AQVILRWDLQ+G++TIPKSTKEHRII NA VF Sbjct: 190 EAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEHRIIANADVF 249 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 +FEL+ DME+ID LNQ+ R GPDPD+FDF Sbjct: 250 NFELTKEDMEKIDALNQNHRVGPDPDNFDF 279 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,595,822,825 Number of extensions: 62731841 Number of successful extensions: 195874 Number of sequences better than 1.0e-05: 5752 Number of HSP's gapped: 185689 Number of HSP's successfully gapped: 5792 Length of query: 270 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 137 Effective length of database: 1,807,701,208 Effective search space: 247655065496 Effective search space used: 247655065496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)