BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0022.p (401 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7Z7J0.1 A7Z7J0_BACA2 YtbD 469 e-130 O34367.1 YTBD_BACSU Uncharacterized MFS-type transporter ytbD 458 e-127 B1D9E1.1 B1D9E1_9BACL Major facilitator superfamily MFS_1 454 e-126 B4BI89.1 B4BI89_9BACI Major facilitator superfamily MFS_1 451 e-125 B3KCF1.1 B3KCF1_9BACI Major facilitator superfamily MFS_1 444 e-123 >A7Z7J0.1 A7Z7J0_BACA2 YtbD Length = 399 Score = 469 bits (1206), Expect = e-130, Method: Compositional matrix adjust. Identities = 247/399 (61%), Positives = 280/399 (70%) Query: 1 MSLDRKKSTXXXXXXXISAFAIGTTEFISVGLLPLISDDLHISVTTAGLTVTLYALGVTF 60 MS R+ +SAFAIGTTEFISVGLLPLIS DLHI VTTAGLTV++YALGVTF Sbjct: 1 MSSHRQSYLPALLALAVSAFAIGTTEFISVGLLPLISQDLHIPVTTAGLTVSMYALGVTF 60 Query: 61 GAPVLTSLTTAISRKTXXXXXXXXFIAGNSLAAAAGGISMLLAARILSALSHGLFMSIAS 120 GAPVLTSLT+ +SRKT FIAGN+LAA+A I +LLAAR++SA SHG+FMSI + Sbjct: 61 GAPVLTSLTSGMSRKTLLLWVMLIFIAGNTLAASASSIGVLLAARVISAFSHGIFMSIGA 120 Query: 121 TIAADLVTEDRRASAISMMFTGLTVATVTGVPLGTFLGQQLGWRXXXXXXXXXXXXXXXX 180 TIAA++V E +RASAIS+MFTGLTVATVTGVP GT+LGQQ GWR Sbjct: 121 TIAANIVPEHKRASAISIMFTGLTVATVTGVPFGTYLGQQFGWRIAFAVIVAVGIIAFLT 180 Query: 181 XXXXXXXXXRKGTRTPLREQVKLVTNGRLLLAFAITALGYGGTFVVFTYLSPLLHEISGF 240 RKGT+T EQVKLVTNGRLLL F ITALGYGGTFVVFTYLSPLL E++GF Sbjct: 181 NALLVPSHLRKGTKTAFSEQVKLVTNGRLLLLFIITALGYGGTFVVFTYLSPLLQEVTGF 240 Query: 241 QEQTVAVILLVYXXXXXXXXXXXXXXXNRKPLNALFYMFAFQAAVLLVLTFTAPFKAAAL 300 Q++TVA ILLVY NR PL ALFYMF QAAVL VLTFTAPFK A L Sbjct: 241 QKETVAAILLVYGIAIAIGNMIGGRLSNRNPLKALFYMFIVQAAVLFVLTFTAPFKTAGL 300 Query: 301 ITIFFMGLLAFMNVPGLQVYVVMLADRFAPSARDVASXXXXXXXXXXXXXXXXXXXXVTD 360 ITI FMGLLAFMNVPGLQVYVVML++RFAPSA D AS +TD Sbjct: 301 ITIIFMGLLAFMNVPGLQVYVVMLSERFAPSAVDTASALNIAAFNAGIAIGSFAGGIITD 360 Query: 361 HLGLVHTPWIGSIMVLGAVVLTAWSRALENRDGRKLQSQ 399 +G++HT WIG++MVL AVVLT WSR LE RD L+ + Sbjct: 361 SIGIIHTAWIGALMVLSAVVLTGWSRKLEKRDRTVLERR 399 >O34367.1 YTBD_BACSU Uncharacterized MFS-type transporter ytbD Length = 396 Score = 458 bits (1178), Expect = e-127, Method: Compositional matrix adjust. Identities = 239/395 (60%), Positives = 278/395 (70%) Query: 1 MSLDRKKSTXXXXXXXISAFAIGTTEFISVGLLPLISDDLHISVTTAGLTVTLYALGVTF 60 M+ K + +SAFAIGTTEFISVGLLPLI+DDL I VTTAGLTV+LYALGVTF Sbjct: 1 MTSANKSNIPALLALAVSAFAIGTTEFISVGLLPLIADDLDIPVTTAGLTVSLYALGVTF 60 Query: 61 GAPVLTSLTTAISRKTXXXXXXXXFIAGNSLAAAAGGISMLLAARILSALSHGLFMSIAS 120 GAP+LTSLT+++SRKT FIAGN++AA A I +LLAAR++SA SHG+FMSI S Sbjct: 61 GAPILTSLTSSMSRKTLLLWIMFIFIAGNTMAATASSIGILLAARVISAFSHGVFMSIGS 120 Query: 121 TIAADLVTEDRRASAISMMFTGLTVATVTGVPLGTFLGQQLGWRXXXXXXXXXXXXXXXX 180 TIAAD+V ED+RASAIS+MFTGLTVATVTGVP GTF+GQQ GWR Sbjct: 121 TIAADIVPEDKRASAISIMFTGLTVATVTGVPFGTFIGQQFGWRFAFMVIIAVGIIAFIT 180 Query: 181 XXXXXXXXXRKGTRTPLREQVKLVTNGRLLLAFAITALGYGGTFVVFTYLSPLLHEISGF 240 RKGT+T +R+Q+KLVTN RLLL F ITALGYGGTFVVFTYLSPLL E++GF Sbjct: 181 NGILVPSKLRKGTKTTMRDQLKLVTNSRLLLLFVITALGYGGTFVVFTYLSPLLQEVTGF 240 Query: 241 QEQTVAVILLVYXXXXXXXXXXXXXXXNRKPLNALFYMFAFQAAVLLVLTFTAPFKAAAL 300 + TVAVILL Y NR P+ ALFYMF QA VL VLTFTAP++AA L Sbjct: 241 KAGTVAVILLGYGIAIAIGNMIGGKLSNRNPIAALFYMFIVQAIVLFVLTFTAPYQAAGL 300 Query: 301 ITIFFMGLLAFMNVPGLQVYVVMLADRFAPSARDVASXXXXXXXXXXXXXXXXXXXXVTD 360 ITI MGLLAFMNVPGLQVYVVMLA+RF PSA DVAS +TD Sbjct: 301 ITILCMGLLAFMNVPGLQVYVVMLAERFVPSAVDVASAMNIAAFNAGIALGSYLGGVITD 360 Query: 361 HLGLVHTPWIGSIMVLGAVVLTAWSRALENRDGRK 395 +GL+HT WIG +MV+GAV+LT WSR +E RD ++ Sbjct: 361 SIGLIHTAWIGGLMVVGAVILTGWSRLMEKRDRQE 395 >B1D9E1.1 B1D9E1_9BACL Major facilitator superfamily MFS_1 Length = 402 Score = 454 bits (1169), Expect = e-126, Method: Compositional matrix adjust. Identities = 235/401 (58%), Positives = 281/401 (70%) Query: 1 MSLDRKKSTXXXXXXXISAFAIGTTEFISVGLLPLISDDLHISVTTAGLTVTLYALGVTF 60 M + +S IS+FAIGTTEFISVGLLPLIS+DL ISVTTAGL+VTLYA+GVT Sbjct: 1 MVTKQSRSALALLALAISSFAIGTTEFISVGLLPLISEDLDISVTTAGLSVTLYAVGVTV 60 Query: 61 GAPVLTSLTTAISRKTXXXXXXXXFIAGNSLAAAAGGISMLLAARILSALSHGLFMSIAS 120 GAP+LT+LT+ + RKT FI GNSLAA+AG I+MLL AR++S+ SHG+FMSI S Sbjct: 61 GAPLLTALTSRVPRKTLLIAIMIIFILGNSLAASAGSIAMLLIARVISSFSHGVFMSIGS 120 Query: 121 TIAADLVTEDRRASAISMMFTGLTVATVTGVPLGTFLGQQLGWRXXXXXXXXXXXXXXXX 180 TIAADLV EDRRASAI+MMF+GLTVATVTGVPLGT LGQ +GWR Sbjct: 121 TIAADLVPEDRRASAIAMMFSGLTVATVTGVPLGTLLGQHMGWRTAFIAIAVIGVIALIS 180 Query: 181 XXXXXXXXXRKGTRTPLREQVKLVTNGRLLLAFAITALGYGGTFVVFTYLSPLLHEISGF 240 RKG RT L E K++ NGRLLLAFAIT LGYGGTFVVFTYLSPLLH+I+GF Sbjct: 181 NLLLIPNTLRKGNRTKLSEPFKVLKNGRLLLAFAITMLGYGGTFVVFTYLSPLLHDITGF 240 Query: 241 QEQTVAVILLVYXXXXXXXXXXXXXXXNRKPLNALFYMFAFQAAVLLVLTFTAPFKAAAL 300 +E T+A IL++Y N+KPL+AL MF QA +LLVLTFTAP+ AAL Sbjct: 241 KESTIAGILVLYGVAIAAGNLIGGKAANKKPLSALLVMFILQAVILLVLTFTAPYAVAAL 300 Query: 301 ITIFFMGLLAFMNVPGLQVYVVMLADRFAPSARDVASXXXXXXXXXXXXXXXXXXXXVTD 360 +TI MGLLAFMNVPGLQ+YVV+LA+R+ P + DV S +TD Sbjct: 301 VTITLMGLLAFMNVPGLQMYVVLLAERYMPKSIDVVSALNISAFNAGIAIGAYVGGIITD 360 Query: 361 HLGLVHTPWIGSIMVLGAVVLTAWSRALENRDGRKLQSQAA 401 LGL+HTPWIG+IMVLGAV LTAWSR+LEN+D + + +A+ Sbjct: 361 ELGLIHTPWIGAIMVLGAVALTAWSRSLENKDTKSKELKAS 401 >B4BI89.1 B4BI89_9BACI Major facilitator superfamily MFS_1 Length = 397 Score = 451 bits (1160), Expect = e-125, Method: Compositional matrix adjust. Identities = 243/396 (61%), Positives = 281/396 (70%) Query: 1 MSLDRKKSTXXXXXXXISAFAIGTTEFISVGLLPLISDDLHISVTTAGLTVTLYALGVTF 60 M+LD+K+ST +SAFAIGTTEFISVGLLPLIS DLH+SV+TAGLTV+LYALGVTF Sbjct: 1 MTLDKKRSTLALLALAVSAFAIGTTEFISVGLLPLISQDLHVSVSTAGLTVSLYALGVTF 60 Query: 61 GAPVLTSLTTAISRKTXXXXXXXXFIAGNSLAAAAGGISMLLAARILSALSHGLFMSIAS 120 GAP+LTS+T+ +SRK+ FI GNS+AA+A I +LL AR+LSA SHG+FMSI S Sbjct: 61 GAPILTSVTSNMSRKSLLLWIMIIFIIGNSIAASATSIGVLLVARVLSAFSHGVFMSIGS 120 Query: 121 TIAADLVTEDRRASAISMMFTGLTVATVTGVPLGTFLGQQLGWRXXXXXXXXXXXXXXXX 180 TIAA LV E+RRASAIS+MFTGLTVAT+TGVP GTF+GQQ GWR Sbjct: 121 TIAASLVPENRRASAISIMFTGLTVATITGVPFGTFIGQQFGWRIAFIFIVVVGIIAFIA 180 Query: 181 XXXXXXXXXRKGTRTPLREQVKLVTNGRLLLAFAITALGYGGTFVVFTYLSPLLHEISGF 240 RKG RT L +Q KL+TNGRLLL F ITALGYGGTFVVFTYLSPLL EI+GF Sbjct: 181 NGILVPSNLRKGARTTLGDQFKLLTNGRLLLLFIITALGYGGTFVVFTYLSPLLQEITGF 240 Query: 241 QEQTVAVILLVYXXXXXXXXXXXXXXXNRKPLNALFYMFAFQAAVLLVLTFTAPFKAAAL 300 E+TVAVILL+Y N+ P+ ALFYMF QA VL +LTFTAPFKAA Sbjct: 241 HEKTVAVILLIYGVAIAIGNVMGGKLSNQNPIGALFYMFIVQAIVLCILTFTAPFKAATF 300 Query: 301 ITIFFMGLLAFMNVPGLQVYVVMLADRFAPSARDVASXXXXXXXXXXXXXXXXXXXXVTD 360 ITI MGLLAFMNVPGLQVYVVMLA+RF PSA DVAS +TD Sbjct: 301 ITIILMGLLAFMNVPGLQVYVVMLAERFVPSAVDVASAINIAAFNAGIAIGSYLGGVITD 360 Query: 361 HLGLVHTPWIGSIMVLGAVVLTAWSRALENRDGRKL 396 GL+HTPWIG++MVLGAV+LT WSR LE +D + + Sbjct: 361 LFGLIHTPWIGALMVLGAVILTDWSRTLERKDQKSV 396 >B3KCF1.1 B3KCF1_9BACI Major facilitator superfamily MFS_1 Length = 405 Score = 444 bits (1142), Expect = e-123, Method: Compositional matrix adjust. Identities = 237/392 (60%), Positives = 274/392 (69%) Query: 1 MSLDRKKSTXXXXXXXISAFAIGTTEFISVGLLPLISDDLHISVTTAGLTVTLYALGVTF 60 M++D+K+S +SAFAIGTTEFISVGLLPLI++D++ISVTTAGLTV++YALGVTF Sbjct: 1 MTIDKKRSLLALIALAVSAFAIGTTEFISVGLLPLIAEDMNISVTTAGLTVSMYALGVTF 60 Query: 61 GAPVLTSLTTAISRKTXXXXXXXXFIAGNSLAAAAGGISMLLAARILSALSHGLFMSIAS 120 GAP+LTSLT+ +SRKT FI GNSLAA+A I +LLAAR+LSA SHG+FMSI S Sbjct: 61 GAPILTSLTSRMSRKTLLLWIMIIFIIGNSLAASAASIGVLLAARVLSAFSHGVFMSIGS 120 Query: 121 TIAADLVTEDRRASAISMMFTGLTVATVTGVPLGTFLGQQLGWRXXXXXXXXXXXXXXXX 180 IAA +V E+RRASAIS+MFTGLTVATVTGVPLGTF+GQQLGWR Sbjct: 121 IIAASVVPENRRASAISIMFTGLTVATVTGVPLGTFIGQQLGWRFAFVAIVAVGIVALIA 180 Query: 181 XXXXXXXXXRKGTRTPLREQVKLVTNGRLLLAFAITALGYGGTFVVFTYLSPLLHEISGF 240 KG RT R+Q KL+TNGRLLL ITALGYGGTFVVFTYLSPLL +I+GF Sbjct: 181 NSILVPSDLPKGERTSFRDQAKLMTNGRLLLMLLITALGYGGTFVVFTYLSPLLQDITGF 240 Query: 241 QEQTVAVILLVYXXXXXXXXXXXXXXXNRKPLNALFYMFAFQAAVLLVLTFTAPFKAAAL 300 E TVAVILLVY NR PL AL YMF QA VL LTFTAPF+AA L Sbjct: 241 NENTVAVILLVYGIAIAVGNVIGGKAANRNPLKALLYMFIAQALVLFALTFTAPFQAAGL 300 Query: 301 ITIFFMGLLAFMNVPGLQVYVVMLADRFAPSARDVASXXXXXXXXXXXXXXXXXXXXVTD 360 ITI MG LAFMNVPGLQVYVVMLA+RFAP A ++AS +T+ Sbjct: 301 ITIILMGFLAFMNVPGLQVYVVMLAERFAPGAVNMASALNIAAFNAGIAIGAFLGGLITN 360 Query: 361 HLGLVHTPWIGSIMVLGAVVLTAWSRALENRD 392 +GL+HT WIG++MV GAVVLT WS LE +D Sbjct: 361 SMGLIHTAWIGALMVAGAVVLTGWSLKLERKD 392 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,620,901,199 Number of extensions: 49371800 Number of successful extensions: 221833 Number of sequences better than 1.0e-05: 2366 Number of HSP's gapped: 220843 Number of HSP's successfully gapped: 2416 Length of query: 401 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 263 Effective length of database: 1,760,596,133 Effective search space: 463036782979 Effective search space used: 463036782979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)