BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0016.p (434 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DH69.1 B1DH69_9BACL Xanthine permease 639 0.0 C0ZJ15.1 C0ZJ15_BREBN Xanthine permease 582 e-164 Q2B416.1 Q2B416_9BACI Xanthine permease 461 e-128 Q65I87.1 Q65I87_BACLD Xanthine permease 443 e-122 A8FEE6.1 A8FEE6_BACP2 NCS2 family uracil:xanthine symporter-2 439 e-121 >B1DH69.1 B1DH69_9BACL Xanthine permease Length = 424 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/416 (75%), Positives = 363/416 (87%) Query: 1 MLSKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQII 60 ML KQ+ LG QHVLAMYAGAV+VPL+VGGAL+L+GTQMAYLIAADLFTCGLAT+LQ++ Sbjct: 1 MLGKQRAFTLGFQHVLAMYAGAVVVPLIVGGALHLNGTQMAYLIAADLFTCGLATILQVL 60 Query: 61 SSKHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFF 120 +K+FGS LPV+LGCTFTAV PIIAIAS SNLATAYGAII+SGLFVVLAAP+YGKLLKFF Sbjct: 61 GTKYFGSRLPVILGCTFTAVGPIIAIASASNLATAYGAIILSGLFVVLAAPLYGKLLKFF 120 Query: 121 PXXXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFM 180 P LSLIPVAMNNVAGGQGSADFG PRN VNRF GF+ Sbjct: 121 PVIVTGSVVTIIGLSLIPVAMNNVAGGQGSADFGLPRNLLLALGTLAVILLVNRFAKGFL 180 Query: 181 RSVSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIV 240 RS+SVL+GL AGT+ GYAMGIV F+ V ASW ++ QPFYFG P+FS+TA+FTMIIVNIV Sbjct: 181 RSISVLIGLAAGTIAGYAMGIVSFAPVSDASWFNMVQPFYFGTPQFSLTAVFTMIIVNIV 240 Query: 241 SMVESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLT 300 SMVESTGVY AVG+ATDQ+VEQKQ++NGLRSEG AIMLGGLFNAFPYTAFSQNVGL++LT Sbjct: 241 SMVESTGVYLAVGRATDQKVEQKQIINGLRSEGAAIMLGGLFNAFPYTAFSQNVGLITLT 300 Query: 301 RVKTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDL 360 RVKTR+VIFAAGGIMVVLGL+PKLAA+TTV+PNAVLGGAM+VMFGSVAASG+SILSEV+L Sbjct: 301 RVKTRDVIFAAGGIMVVLGLIPKLAAITTVIPNAVLGGAMVVMFGSVAASGISILSEVNL 360 Query: 361 RKDGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLS 416 R++ NLLI ACSIAVGLGS+ +P +FDQLP+FA+MLLQNGIV+GS+TAI++N+ LS Sbjct: 361 REERNLLIVACSIAVGLGSSAVPQVFDQLPDFAKMLLQNGIVTGSVTAILMNLLLS 416 >C0ZJ15.1 C0ZJ15_BREBN Xanthine permease Length = 436 Score = 582 bits (1501), Expect = e-164, Method: Compositional matrix adjust. Identities = 294/434 (67%), Positives = 350/434 (80%), Gaps = 4/434 (0%) Query: 1 MLSKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQII 60 ML+K KI+ LGLQH+LAMYAGAV+VPL++GGALNL+ TQ+AYLIAADLFTCG+ATLLQ++ Sbjct: 1 MLAKHKIVTLGLQHILAMYAGAVVVPLIIGGALNLTPTQIAYLIAADLFTCGIATLLQVL 60 Query: 61 SSKHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFF 120 +++ G LPVVLGCTFTAV PIIAIAS SNLATAYGAIIISG+FVVLAAP++GKLL+FF Sbjct: 61 GTRYTGIRLPVVLGCTFTAVGPIIAIASTSNLATAYGAIIISGIFVVLAAPLFGKLLRFF 120 Query: 121 PXXXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFM 180 P LSLIPVAMNN AGGQG DFG P N +NRF TGF+ Sbjct: 121 PTVVQGSVVTIIGLSLIPVAMNNAAGGQGMPDFGQPHNLLLALGTLVIILLINRFFTGFI 180 Query: 181 RSVSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIV 240 R++SVL+GL+ GT + Y MG+V F+ V ASW S+ +PFYFG P+FSI AI TMI+VNI+ Sbjct: 181 RAISVLLGLIVGTAVAYTMGMVSFANVAEASWFSVVEPFYFGTPQFSIVAIVTMILVNII 240 Query: 241 SMVESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLT 300 SM ESTGVYFA+GK T+ +V VV GLR EG+AI+LGG+FNAFPYTAFSQNVGL+SLT Sbjct: 241 SMAESTGVYFALGKVTNTKVTDHDVVKGLRGEGVAIVLGGIFNAFPYTAFSQNVGLVSLT 300 Query: 301 RVKTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDL 360 +K+R+V+ AGGI+VVLGLLPKLAALTTV+PNAVLGGAMI MFG V ASG++ILS+VDL Sbjct: 301 GIKSRDVMIGAGGILVVLGLLPKLAALTTVIPNAVLGGAMIAMFGMVVASGINILSQVDL 360 Query: 361 RKDGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLSRTQE 420 K+ NLLIAACSIAVGLGSA +P+MFDQLP A+M+LQNGIV+GSLTA+ILNI L T + Sbjct: 361 SKNENLLIAACSIAVGLGSAAVPTMFDQLPTLAKMMLQNGIVTGSLTAVILNILLVHTNK 420 Query: 421 NA---APA-ATVPE 430 A APA TV E Sbjct: 421 KASQTAPANVTVSE 434 >Q2B416.1 Q2B416_9BACI Xanthine permease Length = 435 Score = 461 bits (1185), Expect = e-128, Method: Compositional matrix adjust. Identities = 234/428 (54%), Positives = 305/428 (71%) Query: 3 SKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIISS 62 S KI +LG+QHVLAMYAGAVIVPL+VGGAL LSG ++ YL++ D+ CG+AT+LQ+ + Sbjct: 4 SPFKIASLGIQHVLAMYAGAVIVPLIVGGALGLSGEELTYLVSIDILMCGVATILQVWRN 63 Query: 63 KHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFPX 122 + FG GLPVVLGCTFTAVSP+IAI + YG+I++SGLFV+L +G L++FFP Sbjct: 64 QFFGIGLPVVLGCTFTAVSPMIAIGGQYGITAIYGSILVSGLFVILVGKYFGNLVRFFPP 123 Query: 123 XXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMRS 182 ++LIPVAMNN+AGG+GSAD+G+ N + R TGF+RS Sbjct: 124 VVTGSVVTIIGITLIPVAMNNMAGGEGSADYGSLSNLALAFGTLFFIIILYRLFTGFIRS 183 Query: 183 VSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVSM 242 VS+L+GL AGT + MG FS VG ASW + QPFYFG P F AI TMII+ +VS+ Sbjct: 184 VSILLGLFAGTAAAFLMGKSDFSAVGEASWFHMVQPFYFGMPTFEPAAILTMIIIAMVSL 243 Query: 243 VESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTRV 302 VESTGVYFA+G +++++K + NG R+EGLAI+LGGLFNAFPYT +SQNVGLL L+ V Sbjct: 244 VESTGVYFALGDICGKKLDKKDLANGYRAEGLAIVLGGLFNAFPYTTYSQNVGLLQLSGV 303 Query: 303 KTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLRK 362 KT+NVI+ AG +V+LGL+PK+ A+TTV+P+AVLGGAMI MFG V A G+ +LS+VD Sbjct: 304 KTKNVIYTAGVFLVLLGLVPKIGAMTTVIPSAVLGGAMIAMFGMVIAYGIKMLSQVDFSS 363 Query: 363 DGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLSRTQENA 422 NLLI ACS+ +GLG +P +F ++PE R+L NGIV+GSLTAI LN+ + A Sbjct: 364 QENLLIIACSVGMGLGVTAVPGLFAEMPESIRILTDNGIVAGSLTAIALNLVFNVKTSKA 423 Query: 423 APAATVPE 430 AA+ E Sbjct: 424 KTAASKLE 431 >Q65I87.1 Q65I87_BACLD Xanthine permease Length = 438 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 231/434 (53%), Positives = 304/434 (70%), Gaps = 7/434 (1%) Query: 2 LSKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIIS 61 L+ K ++L +QHVLAMYAGAV+VPL+VGGAL L+ Q+ YL++AD+ CG+ATLLQ+ Sbjct: 3 LTAGKTVSLSIQHVLAMYAGAVVVPLIVGGALGLTPAQLTYLVSADILMCGVATLLQVWK 62 Query: 62 SKHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFP 121 ++ FG GLPVVLGCTFTAVSP+I+IAS + YG+II G+ VV+ + +GKL+KFFP Sbjct: 63 NRFFGIGLPVVLGCTFTAVSPMISIASTYGIPAIYGSIIAGGVIVVIISFFFGKLVKFFP 122 Query: 122 XXXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMR 181 ++LIPVAMNN+AGG+GS+DFG N + RFT+GF++ Sbjct: 123 PVVTGSVVTVIGITLIPVAMNNMAGGEGSSDFGELSNLALAFSVLLVIVLLYRFTSGFIK 182 Query: 182 SVSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVS 241 S+S+L+GL+AGT MG V F+ V +A WV + PFYFG P F +T I TM +V IVS Sbjct: 183 SISILIGLIAGTAGAALMGKVDFTEVANAGWVQMIHPFYFGAPVFEVTPILTMSLVLIVS 242 Query: 242 MVESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTR 301 +VESTGVYFA+G TD+++ +K + G R+EG+A++LGG+FNAFPYTA+SQNVGL+ LT Sbjct: 243 LVESTGVYFALGDLTDRRLTEKDLGKGYRAEGIAVLLGGVFNAFPYTAYSQNVGLVQLTG 302 Query: 302 VKTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLR 361 VK +VI AG +++LGL PK AALTT++P VLGGAM+ MFG V A G+ +LS VD Sbjct: 303 VKKNSVIALAGAFLILLGLFPKAAALTTIIPKPVLGGAMVAMFGMVIAYGIKMLSRVDFG 362 Query: 362 KDGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILN-IF----LS 416 K NLLI ACSI +GLG V+P MF LP+ ++L +NGIV+GS TAI+LN IF S Sbjct: 363 KQENLLIVACSIGIGLGVTVVPQMFQNLPDAVKLLTENGIVAGSFTAILLNAIFHIHPFS 422 Query: 417 RTQENAAPAATVPE 430 R E P A E Sbjct: 423 RHAEQ--PEAVSAE 434 >A8FEE6.1 A8FEE6_BACP2 NCS2 family uracil:xanthine symporter-2 Length = 439 Score = 439 bits (1128), Expect = e-121, Method: Compositional matrix adjust. Identities = 217/411 (52%), Positives = 292/411 (71%) Query: 3 SKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIISS 62 K K L+LG+QHVLAMYAGAV+VPL+VG AL L+ Q+ YLI+AD+F CG ATLLQ+ + Sbjct: 5 KKAKTLSLGIQHVLAMYAGAVLVPLIVGDALGLTPAQLTYLISADIFMCGAATLLQLWKN 64 Query: 63 KHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFPX 122 + FG GLPVVLGCTFTAVSP+IAI S +++ YG+II SG ++L + +GKL+KFFP Sbjct: 65 RFFGIGLPVVLGCTFTAVSPMIAIGSKYGISSIYGSIIASGCIIILLSFFFGKLVKFFPP 124 Query: 123 XXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMRS 182 ++LIPVAMNN+AGG+GSAD+G+ N + RFTTGFM++ Sbjct: 125 VVTGSVVTIIGITLIPVAMNNMAGGEGSADYGSFENLGLAFLVLFIIVLLYRFTTGFMKA 184 Query: 183 VSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVSM 242 +++L+G++ GT + MG V + V +A + +PFYFG P F I TM +V IVS+ Sbjct: 185 IAILIGILLGTAVAAFMGKVETAEVANAQVFRMIEPFYFGMPTFEFAPIMTMTLVAIVSL 244 Query: 243 VESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTRV 302 VESTGVYFA+G T++ + +K + G R+EG+A++LGG+FNAFPYTA+SQNVGL+ LT V Sbjct: 245 VESTGVYFALGDLTNRSLNEKDLAKGYRAEGIAVLLGGIFNAFPYTAYSQNVGLIQLTGV 304 Query: 303 KTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLRK 362 K VI G ++++ GL PK+AA TT++P +VLGGAM+ MFG V A G+ +LS VD K Sbjct: 305 KKNQVIVVTGALLMLFGLFPKIAAFTTIIPKSVLGGAMVAMFGMVIAYGIKMLSRVDFAK 364 Query: 363 DGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNI 413 NLLI ACS+ +GLG V+P MFD LPE ++L NGIV+GS TAI+L+I Sbjct: 365 QENLLIVACSVGIGLGVTVVPQMFDHLPESIKLLTSNGIVAGSFTAILLHI 415 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,217,824,432 Number of extensions: 84827451 Number of successful extensions: 404691 Number of sequences better than 1.0e-05: 2700 Number of HSP's gapped: 397354 Number of HSP's successfully gapped: 2740 Length of query: 434 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 295 Effective length of database: 1,751,175,118 Effective search space: 516596659810 Effective search space used: 516596659810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)