BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0013.p (449 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D823.1 B1D823_9BACL Major facilitator superfamily MFS_1 486 e-135 A6CMR7.1 A6CMR7_9BACI Tetracycline resistence protein TETA(L)/TETK 214 2e-53 C0Z634.1 C0Z634_BREBN Probable tetracycline resistance protein 143 3e-32 B3K9N7.1 B3K9N7_9BACI Major facilitator superfamily MFS_1 133 3e-29 Q6HMC4.1 Q6HMC4_BACHK Major facilitator family transporter; poss... 128 1e-27 >B1D823.1 B1D823_9BACL Major facilitator superfamily MFS_1 Length = 446 Score = 486 bits (1251), Expect = e-135, Method: Compositional matrix adjust. Identities = 243/415 (58%), Positives = 304/415 (73%), Gaps = 2/415 (0%) Query: 1 VNDKVVMPLWTFCLFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIY 60 +ND++VMP+W LF+VVMNTTMFNVS+P+II DL I++DLGSW++SSYSIGYALSTVIY Sbjct: 1 MNDRIVMPVWMLGLFVVVMNTTMFNVSVPSIINDLGITSDLGSWIVSSYSIGYALSTVIY 60 Query: 61 SRLSDXXXXXXXXXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSR 120 SRLSD +SV+G+FAH+F +LL RILQS SR Sbjct: 61 SRLSDIAPIRRLVAVGLLILGLSSVLGIFAHNFALLLTARILQSAGAGTMAGLGLVLASR 120 Query: 121 YVPVARRGATIAMISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXX 180 Y+PV RRG I+MI+ GSAMAFGLGPIVGG+IS+YFGWNGLF VTC Sbjct: 121 YIPVERRGRAISMIAAGSAMAFGLGPIVGGVISEYFGWNGLFVVTCLVLVILPVLLKLLP 180 Query: 181 KEQPQAAVFDLYGAALTVINAGSLLVAVTSQSYIWLAVSVASFAVHAIHLRRARD--SFI 238 KE PQ A FD +GAA TVINA SLL+AVT QS IWL SV + +H +H+R+A+ +F+ Sbjct: 181 KENPQPASFDYWGAAWTVINATSLLIAVTQQSVIWLLASVITIVIHVLHIRKAQGNGTFV 240 Query: 239 NPKLLQMPGYLKLTAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFL 298 NP++ + PGY++L +GF +L++NLGNLFLMPL L+NLFH+S ++IG IAPGAILSA L Sbjct: 241 NPEVFRQPGYIRLLVVGFCLLIINLGNLFLMPLVLANLFHKSEMLIGFLIAPGAILSAVL 300 Query: 299 TRFVGRWIDRYGNLRFLLIGHGILATVLAVFALGLQVSPLVLLFGYLCFSPAFSATMASL 358 T FVGRW+DRYGN+RFL IG +L V+A+F L SP V++ GYL FSPAFSAT+ASL Sbjct: 301 TPFVGRWLDRYGNVRFLFIGTAVLTVVMALFMTALGSSPFVIMCGYLLFSPAFSATLASL 360 Query: 359 NNEASGILPRTWIGSGMGLLQLIQFFGGSVSVAVCGLLLHAQRTLAPSGAYRNVY 413 NN+ S ILPR IGSGMG++QLIQFFGGS+SVA+CG+LLH ++ AY +VY Sbjct: 361 NNQTSSILPRHLIGSGMGMMQLIQFFGGSLSVAICGVLLHKMSSITLEHAYNHVY 415 >A6CMR7.1 A6CMR7_9BACI Tetracycline resistence protein TETA(L)/TETK Length = 444 Score = 214 bits (544), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 139/403 (34%), Positives = 215/403 (53%) Query: 11 TFCLFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRLSDXXXXX 70 + +++VVMNTTMFNV+LP I+ D ISA G+WV+S YSI A+ T+ Y+RLSD Sbjct: 20 SLAIWLVVMNTTMFNVALPNILNDFSISASEGAWVVSGYSIVLAICTITYTRLSDYLPIR 79 Query: 71 XXXXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVPVARRGAT 130 +S+IG FA S+++LL+ R++Q+ SRY+P++ RG Sbjct: 80 RLIAIGISLFGLSSIIGFFAESYSLLLLARLMQATGAAAIPGLSMVFASRYIPLSTRGRA 139 Query: 131 IAMISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKEQPQAAVFD 190 +AMI+ S++ FGLGP+VGG+I++Y WN LF VT KE+ FD Sbjct: 140 MAMIASASSLGFGLGPVVGGMITEYLAWNYLFIVTLMVIALIPVLLRILPKEEVAKGKFD 199 Query: 191 LYGAALTVINAGSLLVAVTSQSYIWLAVSVASFAVHAIHLRRARDSFINPKLLQMPGYLK 250 GA LT ++ + L+ +++ ++ +L + + + + R+ FI P L++ Y Sbjct: 200 FIGAVLTGLSVTAFLLFLSTFNWPFLLAGILLLGLLWLRINRSALPFIQPDLIKDKRYRL 259 Query: 251 LTAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFLTRFVGRWIDRYG 310 L I F + + L LMPL L ++F ++ IG I PGA+ SA +VGR IDRYG Sbjct: 260 LLCISFLGFLTHFAILLLMPLMLGSIFSKAPSSIGFIIFPGAMFSAVAAIYVGRLIDRYG 319 Query: 311 NLRFLLIGHGILATVLAVFALGLQVSPLVLLFGYLCFSPAFSATMASLNNEASGILPRTW 370 N+ L H +L +F +S ++ GY+ S FS+ +S NE S IL + + Sbjct: 320 NITVLFGAHVLLLISTLIFYFLSPLSEYWIMAGYMFTSFGFSSLSSSSTNETSRILSKEY 379 Query: 371 IGSGMGLLQLIQFFGGSVSVAVCGLLLHAQRTLAPSGAYRNVY 413 +GSG+G+ QL F G + V +LL +G ++ Y Sbjct: 380 VGSGIGMKQLTHFVGSASGSVVGAILLEWHGQGFSTGDFQRSY 422 >C0Z634.1 C0Z634_BREBN Probable tetracycline resistance protein Length = 455 Score = 143 bits (361), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 115/395 (29%), Positives = 185/395 (46%), Gaps = 8/395 (2%) Query: 4 KVVMPLWTFCLFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRL 63 + V+ + F L I VM+ TMFN+ LP I + H+S SWV S++ + YA+ TV+Y +L Sbjct: 11 EKVLRVLVFTLMITVMSATMFNIVLPQIRTEFHLSFSQVSWVTSAFLLIYAVGTVMYGKL 70 Query: 64 SDXXXXXXXXXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVP 123 +D SVIG FA ++ ++L RI+Q+ RY P Sbjct: 71 ADSYKLKHLLTFGLILFAIGSVIGFFADNYGMVLFGRIVQAAGAAVVPATSSIIPVRYFP 130 Query: 124 VARRGATIAMISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKEQ 183 RG + + G A+ LGP++ L+ W LF + +E+ Sbjct: 131 PESRGRALGISMTGMAIGSALGPVLSSLLVSLLDWRWLFCMPLFTLFAVPFFRKYLEEEE 190 Query: 184 PQAAVFDLYGAALTVINAGSLLVAVTSQSYIWLAVSVASFAVHAIHLRRARDSFINPKLL 243 +A D G L I LL+A+T+QS + + SF + + +R A + FI P L Sbjct: 191 REAVKMDWIGGGLLAITIALLLLAITNQSLLLALGCILSFLLFLVRIRTAAEPFIRPGLF 250 Query: 244 QMPGYLKLTAIGFSILVLNLGN----LFLMPLALSNLFHQSALMIGLYIAPGAILSAFLT 299 + Y ++G I L +G+ FL P L+++ H + +IG + PGAI++AFL Sbjct: 251 RSKRY----SLGLIIAFLMIGSGYSFAFLSPQLLADVNHLAPGLIGFVMVPGAIVTAFLG 306 Query: 300 RFVGRWIDRYGNLRFLLIGHGILATVLAVFALGLQVSPLVLLFGYLCFSPAFSATMASLN 359 + G D+ GN +L + +F+ VSP + + + S M S++ Sbjct: 307 KKAGILADKKGNSYLFYTASALLLSSFVLFSSFAGVSPFFIAVFLIFGNVGLSFMMISMS 366 Query: 360 NEASGILPRTWIGSGMGLLQLIQFFGGSVSVAVCG 394 N S LP+ IG GMGLL ++ F G+++ + G Sbjct: 367 NAISQSLPKEQIGVGMGLLAMLNFIAGALAATLYG 401 >B3K9N7.1 B3K9N7_9BACI Major facilitator superfamily MFS_1 Length = 464 Score = 133 bits (335), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 110/387 (28%), Positives = 170/387 (43%), Gaps = 8/387 (2%) Query: 12 FCLFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRLSDXXXXXX 71 F + + M+ TMFN+ LP I Q+ +S SWV S Y + YA+ +V+Y +L+D Sbjct: 21 FTMILSSMSATMFNIVLPEISQEFRLSFAQVSWVSSIYMLIYAIGSVLYGKLADTYKLKN 80 Query: 72 XXXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVPVARRGATI 131 S+ GL A ++ ++L+ RILQ+ RY P RG + Sbjct: 81 LVTFGLLLFFVGSITGLAAQAYWMVLLGRILQAAGAAVIPAIAMIIPVRYFPAENRGRAL 140 Query: 132 AMISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKEQPQAAVFDL 191 + + G A+ +GP+V L+ F W LF + E+ Q A D Sbjct: 141 GITATGLAIGNAIGPVVSALVVSTFHWRWLFFIPLLILVTLPLYRKYLGNEKGQGARIDW 200 Query: 192 YGAALTVINAGSLLVAVTSQSYIWLAVSVASFAVHAIHLRRARDSFINPKLLQMPGYLKL 251 G L LL+AVT +I+ S+ F + +R A+ F+ P+L + Y Sbjct: 201 LGGGLLAGTVALLLLAVTQGGWIFGLGSLILFLAFLLRIRSAKAPFVQPRLFRNQSYTVG 260 Query: 252 TAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFLTRFVGRWIDRYGN 311 +G I+ + FL P LS+L + IG + PGA+++A L G+ D G Sbjct: 261 LVLGMFIMSVGYALPFLTPQLLSDLNGLAPGWIGFVMVPGAVVTAMLGGRGGKLADTKGT 320 Query: 312 LRFLLIGHGILATVL----AVFALGLQVSPLVLLFGYLCFSPAFSATMASLNNEASGILP 367 RFL IL + + FA VSPL++ + + SL+ S LP Sbjct: 321 -RFLFYAASILLLLCFALLSTFA---GVSPLLIALFLIFGNVGIMFMQISLSKTISLSLP 376 Query: 368 RTWIGSGMGLLQLIQFFGGSVSVAVCG 394 + G GMGL L+ F G+++ V G Sbjct: 377 KEQTGVGMGLFSLLNFLSGAIATGVYG 403 >Q6HMC4.1 Q6HMC4_BACHK Major facilitator family transporter; possible metal-tetracycline/H+ antiporter Length = 463 Score = 128 bits (321), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 108/419 (25%), Positives = 188/419 (44%), Gaps = 23/419 (5%) Query: 13 CLFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRLSDXXXXXXX 72 L VMN TMFNV+LP I ++ ++ SW+++SY + YA+ +++ +L+D Sbjct: 19 TLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDL 78 Query: 73 XXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVPVARRGATIA 132 S++GL A + V+++ R++Q+ RY +RG + Sbjct: 79 LTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALG 138 Query: 133 MISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKEQPQAAVFDLY 192 +VG A+ LGP+ GLI+ + W +F ++ + +A D+ Sbjct: 139 TSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDIL 198 Query: 193 GAALTVINAGSLLVAVTSQSYIWLAVSVASFAVHAIHLRRARDSFINPKLLQMPGYLKLT 252 G L ++ L+A+T + A+ A + +R+A++ FI P L + + Sbjct: 199 GGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAKEPFIKPILFKNKNFSIGL 258 Query: 253 AIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFLTRFVGRWIDRYGNL 312 + F ++ F+ P LS + + IG + P AI SA + R GR D GN Sbjct: 259 LLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNF 318 Query: 313 R-------FLLIGHGILATVLAVFALGLQVSPLVLLFGYLCFSPAFSATMASLNNEASGI 365 F+ + +L+T + V A V L+L+FG + + S++N S Sbjct: 319 ALVFIASVFIFLAFSLLSTFIGVSAF---VIALILIFGNV----GQTFMQISMSNTISQT 371 Query: 366 LPRTWIGSGMGLLQLIQFFGGSVSVAVCGLLLHAQRT---LAP------SGAYRNVYGI 415 L + G GMGLL +I F G+++++V G LL T L P + Y N++G+ Sbjct: 372 LSKEETGVGMGLLSMINFISGAMAMSVVGKLLDKGSTSLKLNPFVANEAANMYSNIFGV 430 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,115,988,500 Number of extensions: 76069956 Number of successful extensions: 301476 Number of sequences better than 1.0e-05: 4923 Number of HSP's gapped: 298445 Number of HSP's successfully gapped: 4925 Length of query: 449 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 310 Effective length of database: 1,751,175,118 Effective search space: 542864286580 Effective search space used: 542864286580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)