BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0010.p (405 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJA3.1 B3KJA3_9BACI Glutathionylspermidine synthase 586 e-165 B1DBS4.1 B1DBS4_9BACL Glutathionylspermidine synthase 522 e-146 C2TYX0.1 C2TYX0_BACCE Glutathionylspermidine synthase 169 6e-40 C2VMC6.1 C2VMC6_BACCE Glutathionylspermidine synthase 168 8e-40 C2UWR9.1 C2UWR9_BACCE Glutathionylspermidine synthase 166 5e-39 >B3KJA3.1 B3KJA3_9BACI Glutathionylspermidine synthase Length = 399 Score = 586 bits (1511), Expect = e-165, Method: Compositional matrix adjust. Identities = 274/396 (69%), Positives = 326/396 (82%) Query: 5 DSVFEYLDQSGLERAPRVEQLHKLGFTWADLEDEEYWLDAVAVMRGETYKELEEASVKLW 64 +SVF R RV +L +LGFTWA+LE E YW+D + +R ETY+ELEEA+ LW Sbjct: 4 ESVFTVSGLPDPNRNGRVSELAELGFTWANLEGEAYWIDQIVTIRQETYRELEEAAAVLW 63 Query: 65 SILDKAVRYVHRRHDLYDLLGIPPVLWQMLDDTPLPEPGLISRYARFDFAIAGDGTIKLL 124 + LDK VRYVHR H +Y L+GIPP+LWQMLD+ PLPE GLISRYARFDFAIA DG+IKLL Sbjct: 64 ATLDKTVRYVHRNHHIYSLIGIPPILWQMLDECPLPEEGLISRYARFDFAIAHDGSIKLL 123 Query: 125 ELNADTPTGYVEASVATPWICGQAGISSRNNGMKERLAAAWSIEQPDTAACVAYGTHQED 184 ELNADTPTGYVEAS+ATPW+C QAGI S N M+E +A AW+ E+PDTAAC+AYG H ED Sbjct: 124 ELNADTPTGYVEASIATPWMCAQAGIYSPNERMQELVAQAWAAERPDTAACIAYGEHLED 183 Query: 185 SGTIEALVAHSGLDIRCVDCLDLWIDEGTVRDAENRIIKRMFALYPKEWMAVDDGGEALA 244 SGTIEALV HSGLDI+ VDCLDLW+DEG ++D E R I RMFALYPKEWM+VDDGG+ALA Sbjct: 184 SGTIEALVRHSGLDIKLVDCLDLWVDEGMLKDGEGREIHRMFALYPKEWMSVDDGGDALA 243 Query: 245 YAIESGQLQLFNGPHSILLQSKGLIAAVWGMYELGLLFSEDERQAIASYILPTYNKPXXX 304 YAIE+G+LQLFN PHSILLQSKGL+AA+WGMYELGLLF E ER+AI Y+LPTYNKP Sbjct: 244 YAIETGRLQLFNSPHSILLQSKGLLAAMWGMYELGLLFDEQEREAIVKYMLPTYNKPVFS 303 Query: 305 XXXXXXXXXXREGGSVRIFDDSGALELADEDGFDSSVLFPTVYQKRAELARIQTPEGELH 364 REGGSV+++D +G LE+ D+DG+D+SV+FPTVYQ+RAELAR++T EGE H Sbjct: 304 GNFVSKSMFGREGGSVKLYDQTGQLEVEDQDGYDTSVMFPTVYQRRAELARVETAEGEFH 363 Query: 365 LLTGMFVINGVPCGLLGRAGGPITGNASHFIALGVR 400 LLTG+FV+NG+PCGLLGRAGG ITGN+SHFIA+GV+ Sbjct: 364 LLTGLFVLNGMPCGLLGRAGGLITGNSSHFIAMGVK 399 >B1DBS4.1 B1DBS4_9BACL Glutathionylspermidine synthase Length = 410 Score = 522 bits (1344), Expect = e-146, Method: Compositional matrix adjust. Identities = 246/405 (60%), Positives = 306/405 (75%) Query: 1 MSPADSVFEYLDQSGLERAPRVEQLHKLGFTWADLEDEEYWLDAVAVMRGETYKELEEAS 60 M+ VF + + +R +V L +GF+WADLE E YW+D V M Y+ELE+AS Sbjct: 1 MTITTEVFTVIGEPDTDRTRKVGILKDMGFSWADLESETYWIDQVVGMDRTIYEELEQAS 60 Query: 61 VKLWSILDKAVRYVHRRHDLYDLLGIPPVLWQMLDDTPLPEPGLISRYARFDFAIAGDGT 120 +LW ILDK RY+H +HDLY L+GIP +LW ++D P+PE G++SRYARFD+AI+ DG+ Sbjct: 61 RELWLILDKTARYIHGKHDLYQLIGIPTILWDLIDSLPMPEKGVMSRYARFDYAISPDGS 120 Query: 121 IKLLELNADTPTGYVEASVATPWICGQAGISSRNNGMKERLAAAWSIEQPDTAACVAYGT 180 IK+LELNADTPTGYVEAS+ATPW+C AGI + N MKE +A+AW+IE+PDT ACVAYG+ Sbjct: 121 IKMLELNADTPTGYVEASIATPWLCELAGIRNVNGKMKELVASAWAIERPDTVACVAYGS 180 Query: 181 HQEDSGTIEALVAHSGLDIRCVDCLDLWIDEGTVRDAENRIIKRMFALYPKEWMAVDDGG 240 H EDSGTIEAL HSGL++R DCL+LW+DEG +++ + + I RMFALYPKEWMA DDGG Sbjct: 181 HLEDSGTIEALAKHSGLNVRLEDCLNLWVDEGILKNGQGKPIHRMFALYPKEWMAHDDGG 240 Query: 241 EALAYAIESGQLQLFNGPHSILLQSKGLIAAVWGMYELGLLFSEDERQAIASYILPTYNK 300 EALAYA+E G L LFN HSILLQSKGL AA WG+YEL +LF + ER+ I Y+LPTYNK Sbjct: 241 EALAYAVEKGYLNLFNSVHSILLQSKGLQAAAWGLYELDVLFDKHERETIERYMLPTYNK 300 Query: 301 PXXXXXXXXXXXXXREGGSVRIFDDSGALELADEDGFDSSVLFPTVYQKRAELARIQTPE 360 P REGGSV++FD SG LE+ D++ FD+S LFP VYQKR L+RI+T Sbjct: 301 PVFDGDFVSKSMFGREGGSVKMFDQSGELEMEDQEAFDTSELFPVVYQKRTRLSRIRTAA 360 Query: 361 GELHLLTGMFVINGVPCGLLGRAGGPITGNASHFIALGVRELEDE 405 GELHLL GMF+ING GLLGRAGGPITGN+SHFI +GV E ++E Sbjct: 361 GELHLLVGMFMINGETAGLLGRAGGPITGNSSHFIPIGVLEPDEE 405 >C2TYX0.1 C2TYX0_BACCE Glutathionylspermidine synthase Length = 415 Score = 169 bits (427), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 120/403 (29%), Positives = 194/403 (48%), Gaps = 34/403 (8%) Query: 23 EQLHKLGFTWADLEDEEYWLDAVAV----MRGETYKELEEASVKLWSILDKAVRYVHRRH 78 E+ + GFTW L + E W +A M TY + +A+ ++ +L + +Y+ Sbjct: 19 EEAGRNGFTWPSLLENEEWNQYMATGMFRMSVNTYTAISKATEEIMYVLYRTYQYIVHTT 78 Query: 79 DLYDLLGIPPVLWQMLDDTPLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTGYVEAS 138 + + LG PP W++ + GL S + R DF + GD IKL+E+N DTPTGY+E S Sbjct: 79 EDFQKLGFPPETWEI---ARMKHTGLFSYFTRLDFIVNGD-VIKLIEVNCDTPTGYLEPS 134 Query: 139 VATPWICGQAGISSRNNGMKERLAAAW-------SIEQPDTAACVAYGTHQEDSGTIEAL 191 VA +C ++ N+ ++E + AW +I+ +T +Y H ED T++ L Sbjct: 135 VANEVLCRYHDVNHPNH-IEEHIVQAWEQIKHDYNIDPEETIYFSSYDWHDEDHQTVQFL 193 Query: 192 VAHS-GLDIRCVDCLDLWIDEGTVRDAENRIIKRMFALYPKEWMA--VDDGGEALAYA-- 246 ++ V D+ + + + + I ++ LYP E++ VD G+ + Sbjct: 194 RSYCLNQPTEYVGIQDIVVTDDGIYTPNGKRIHYLYRLYPIEYLVTDVDKNGKRIGLQFL 253 Query: 247 --IESGQLQLFNGPHSILLQSKGLIAAVWGMYELGLLFSEDERQAIASYILPTY--NKPX 302 I G++++ N P + ++Q+K ++A +W +YE + F E+ER I Y LPTY NKP Sbjct: 254 NHIAQGRVKIINPPAAFVMQNKNMLALIWKLYEKAVFFEEEERVIINKYFLPTYFTNKPF 313 Query: 303 XXXXXXXXXX--XXREGGSVRIFDDSGALELADEDGFDSSVLFPTVYQKRAELA--RIQT 358 REGG V I+++ EL ED + +YQ+ E+ I T Sbjct: 314 KERNESFVSKPLYGREGGGVSIYEND---ELLAEDKTEYYFEQRKIYQQYIEMPDYTIDT 370 Query: 359 PEGEL--HLLTGMFVINGVPCGLLGRAGGPITGNASHFIALGV 399 +G LL G I+G GL R G ITGN S F + + Sbjct: 371 WDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTI 413 >C2VMC6.1 C2VMC6_BACCE Glutathionylspermidine synthase Length = 415 Score = 168 bits (426), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 120/403 (29%), Positives = 194/403 (48%), Gaps = 34/403 (8%) Query: 23 EQLHKLGFTWADLEDEEYWLDAVAV----MRGETYKELEEASVKLWSILDKAVRYVHRRH 78 E+ + GFTW L + E W +A M TY + +A+ ++ +L + +Y+ Sbjct: 19 EEAGRNGFTWPSLLENEEWNQYMATGMFRMSVNTYTAISKATEEIMYVLYRTYQYIVHTT 78 Query: 79 DLYDLLGIPPVLWQMLDDTPLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTGYVEAS 138 + + LG PP W++ + GL S + R DF + GD IKL+E+N DTPTGY+E S Sbjct: 79 EDFQKLGFPPETWEI---ARMKHTGLFSYFTRLDFIVNGD-VIKLIEVNCDTPTGYLEPS 134 Query: 139 VATPWICGQAGISSRNNGMKERLAAAW-------SIEQPDTAACVAYGTHQEDSGTIEAL 191 VA +C ++ N+ ++E + AW +I+ +T +Y H ED T++ L Sbjct: 135 VANEVLCRYHDVNHPNH-IEEHIVQAWEQIKHDYNIDPEETIYFSSYDWHDEDHQTVQFL 193 Query: 192 VAHS-GLDIRCVDCLDLWIDEGTVRDAENRIIKRMFALYPKEWMA--VDDGGEALAYA-- 246 ++ V D+ + + + + I ++ LYP E++ VD G+ + Sbjct: 194 RSYCLNQPTEYVGIQDIVVTDDGIYTPNGKRIHYLYRLYPIEYLVTDVDKNGKRIGLQFL 253 Query: 247 --IESGQLQLFNGPHSILLQSKGLIAAVWGMYELGLLFSEDERQAIASYILPTY--NKPX 302 I G++++ N P + ++Q+K ++A +W +YE + F E+ER I Y LPTY NKP Sbjct: 254 NHIAQGRVKIINPPAAFVMQNKNVLALIWKLYEKAVFFEEEERVIINKYFLPTYFTNKPF 313 Query: 303 XXXXXXXXXX--XXREGGSVRIFDDSGALELADEDGFDSSVLFPTVYQKRAELA--RIQT 358 REGG V I+++ EL ED + +YQ+ E+ I T Sbjct: 314 KERNESFVSKPLYGREGGGVSIYEND---ELLAEDKTEYYFEQRKIYQQYIEMPDYTIDT 370 Query: 359 PEGEL--HLLTGMFVINGVPCGLLGRAGGPITGNASHFIALGV 399 +G LL G I+G GL R G ITGN S F + + Sbjct: 371 WDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTI 413 >C2UWR9.1 C2UWR9_BACCE Glutathionylspermidine synthase Length = 415 Score = 166 bits (419), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 119/403 (29%), Positives = 193/403 (47%), Gaps = 34/403 (8%) Query: 23 EQLHKLGFTWADLEDEEYWLDAVAV----MRGETYKELEEASVKLWSILDKAVRYVHRRH 78 E+ + GFTW L + E W +A M TY + +A+ ++ +L + +Y+ Sbjct: 19 EEAGRNGFTWPSLLENEEWNQYMATGMFRMSVNTYTAISKATEEIMYVLYRTYQYIVHTT 78 Query: 79 DLYDLLGIPPVLWQMLDDTPLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTGYVEAS 138 + + LG PP W++ + GL S + R DF + GD IKL+E+N DTPTGY+E S Sbjct: 79 EDFQKLGFPPETWEI---ARMKHTGLFSYFTRLDFIVNGD-VIKLIEVNCDTPTGYLEPS 134 Query: 139 VATPWICGQAGISSRNNGMKERLAAAW-------SIEQPDTAACVAYGTHQEDSGTIEAL 191 VA +C ++ N+ ++E + AW +I+ +T +Y H ED T++ L Sbjct: 135 VANEVLCRYHDVNHPNH-IEEHIVQAWEQIKHDYNIDPEETIYFSSYDWHDEDHQTVQFL 193 Query: 192 VAHS-GLDIRCVDCLDLWIDEGTVRDAENRIIKRMFALYPKEWMA--VDDGGEALAYA-- 246 ++ + D+ + + + I ++ LYP E++ VD G+ + Sbjct: 194 RSYCLNQPTEYIGIQDIVVTGDGIYTPNGKRIHYLYRLYPIEYLVTDVDKNGKRIGLQFL 253 Query: 247 --IESGQLQLFNGPHSILLQSKGLIAAVWGMYELGLLFSEDERQAIASYILPTY--NKPX 302 I G++++ N P + ++Q+K ++A +W +YE + F E+ER I Y LPTY NKP Sbjct: 254 NHIARGRVRIINPPAAFVMQNKSVLALIWKLYEKAVFFEEEERGIINKYFLPTYFTNKPF 313 Query: 303 XXXXXXXXXX--XXREGGSVRIFDDSGALELADEDGFDSSVLFPTVYQKRAELA--RIQT 358 REGG V I+++ EL ED + +YQ+ E+ I T Sbjct: 314 TERNESFVSKPLYGREGGGVSIYEND---ELLAEDKTEYYFEQRKIYQQYIEMPDYTIDT 370 Query: 359 PEGEL--HLLTGMFVINGVPCGLLGRAGGPITGNASHFIALGV 399 +G LL G I+G GL R G ITGN S F + + Sbjct: 371 WDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTI 413 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,590,117,670 Number of extensions: 111695304 Number of successful extensions: 236756 Number of sequences better than 1.0e-05: 465 Number of HSP's gapped: 235943 Number of HSP's successfully gapped: 465 Length of query: 405 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 267 Effective length of database: 1,760,596,133 Effective search space: 470079167511 Effective search space used: 470079167511 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)