BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0007.p (337 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJA6.1 B3KJA6_9BACI Ion transport 2 domain protein 504 e-141 B1DK47.1 B1DK47_9BACL Ion transport 2 domain protein 467 e-130 A6CU64.1 A6CU64_9BACI Potassium channel protein 256 2e-66 C4KZ24.1 C4KZ24_EXISA Ion transport 2 domain protein 249 3e-64 B1YEW8.1 B1YEW8_EXIS2 Ion transport 2 domain protein 244 1e-62 >B3KJA6.1 B3KJA6_9BACI Ion transport 2 domain protein Length = 340 Score = 504 bits (1297), Expect = e-141, Method: Compositional matrix adjust. Identities = 228/337 (67%), Positives = 287/337 (85%) Query: 1 MHFLLRLSGKLMRLKKKSIGLIILAFIVISATIAFLLEPGTFSNWFNAFYWVMTTMSTVG 60 MHF LRLS K+MRL+K +I +I++ F+V+ +T+AFLLEP TF +WFNA YWV+TTM+TVG Sbjct: 1 MHFFLRLSLKMMRLRKTTISVIVVLFVVLCSTLAFLLEPETFHHWFNALYWVLTTMATVG 60 Query: 61 YGDFFAGTVAGKLFTIFLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLI 120 YGD++A TV GK+ TIF+YIFGIGLLSLVIGK+I++ G +QR+RGAG L+F G HV++I Sbjct: 61 YGDYYAATVPGKILTIFIYIFGIGLLSLVIGKVIESFGSMQRQRGAGRLNFKGSGHVIII 120 Query: 121 NWNRKTQAAVDEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGA 180 NW++K +A+DEIL Y+P IV+ID+S +HP++ +E VHF+SGDP+SDD+L++A I A Sbjct: 121 NWSKKAMSAIDEILSYSPQSEIVVIDDSSRHPVEHLEQVHFVSGDPASDDVLMRANILEA 180 Query: 181 KAAIVFGDTRIDEASLTDGKTLLIASSIERIAPQVHTTVEIMQEKNIQNFRHVHVNEFVL 240 K+AIVF D RIDE++L DGK+LLI SSIERIAP VHTTVEIMQEK++QNFRH VNEFVL Sbjct: 181 KSAIVFADARIDESALVDGKSLLIVSSIERIAPDVHTTVEIMQEKHVQNFRHNQVNEFVL 240 Query: 241 SHDAISRLAVRSALQEGNSEVITQLLSREHGDDIYEIPRDAAWHTYGDAFQALLRRGATL 300 SHDA+SRLAVR+ALQEGNS+V+ QLLSR+HGDDIYE+ + AW TYGDAFQ LLR+GATL Sbjct: 241 SHDAVSRLAVRAALQEGNSDVLMQLLSRQHGDDIYEVALNPAWKTYGDAFQDLLRQGATL 300 Query: 301 LSDRGDLGINRKLDQPIPQDARLYIVSDEETYRQIKG 337 +SDR DL INRKLD PIP+DARLY+VSDE T +I G Sbjct: 301 ISDRSDLSINRKLDLPIPRDARLYVVSDEATMNRIAG 337 >B1DK47.1 B1DK47_9BACL Ion transport 2 domain protein Length = 338 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 217/335 (64%), Positives = 272/335 (81%) Query: 1 MHFLLRLSGKLMRLKKKSIGLIILAFIVISATIAFLLEPGTFSNWFNAFYWVMTTMSTVG 60 M F LR K++R+ K++I ++++AF+ SATIA+ +EP TF WFNA YWVMTTM+TVG Sbjct: 1 MPFFLRTMMKVLRVPKRTIFIVVIAFVFGSATIAYAIEPTTFHTWFNALYWVMTTMATVG 60 Query: 61 YGDFFAGTVAGKLFTIFLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLI 120 YGD+FA TV GK+FT+F+Y+FGIGLLSLVIGKIID + + + AG L+FHGKDH++LI Sbjct: 61 YGDYFAKTVWGKIFTLFIYVFGIGLLSLVIGKIIDLLSTVYKHWEAGELNFHGKDHIILI 120 Query: 121 NWNRKTQAAVDEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGA 180 NW++K Q A++EIL ++P IV+IDES +HP+ +HFISGDPSS++IL KA ++ A Sbjct: 121 NWSKKAQYAIEEILSFSPTSHIVVIDESDKHPVTGRPEIHFISGDPSSEEILEKAMLKQA 180 Query: 181 KAAIVFGDTRIDEASLTDGKTLLIASSIERIAPQVHTTVEIMQEKNIQNFRHVHVNEFVL 240 KA I+F DTRIDE +L DGKTLLIASSIER+ P +HTTVEI QEKN +NFRHVHVN+FVL Sbjct: 181 KAVIIFADTRIDEPALADGKTLLIASSIERLHPAIHTTVEITQEKNTKNFRHVHVNDFVL 240 Query: 241 SHDAISRLAVRSALQEGNSEVITQLLSREHGDDIYEIPRDAAWHTYGDAFQALLRRGATL 300 S+DA+SRLAVR+ALQ+G+SE+I QL+ RE GDDIYEIP W TY DAFQ L GATL Sbjct: 241 SYDAVSRLAVRAALQQGSSEIINQLIRREVGDDIYEIPVRPEWKTYKDAFQGTLEYGATL 300 Query: 301 LSDRGDLGINRKLDQPIPQDARLYIVSDEETYRQI 335 LSDRGDLGINRKL+ PIP DARLY+VSDE TYR++ Sbjct: 301 LSDRGDLGINRKLNDPIPPDARLYVVSDELTYRKL 335 >A6CU64.1 A6CU64_9BACI Potassium channel protein Length = 330 Score = 256 bits (655), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 127/323 (39%), Positives = 201/323 (62%), Gaps = 6/323 (1%) Query: 18 SIGLIILAFIVISATIAF-LLEPGTFSNWFNAFYWVMTTMSTVGYGDFFAGTVAGKLFTI 76 S+ L++ A IV+ T + +LEP TF F F+W MTT++TVGYGD+F T AG++ + Sbjct: 4 SVILVLSAAIVLFGTFSIHILEPETFPTLFEGFWWTMTTLTTVGYGDYFPVTTAGRILGV 63 Query: 77 FLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLINWNRKTQAAVDEILCY 136 FL+IFGIG+L L+I KI+D + + G L + K H + I W+ +T+ A+ E+L Sbjct: 64 FLFIFGIGILGLLISKIMDVLATYHTLKREGKLVYKNKGHYIYIGWSARTERAIKEVLAN 123 Query: 137 TPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGAKAAIVFGDTRIDEASL 196 D IV++D P + +HFISGDPS+++ L+KA I A +F D I + L Sbjct: 124 RADKEIVLVDHLDSSPFSH-DQLHFISGDPSNEETLMKANITEADRVAIFSDMSITQPVL 182 Query: 197 TDGKTLLIASSIERIAPQ----VHTTVEIMQEKNIQNFRHVHVNEFVLSHDAISRLAVRS 252 DGK+LLIAS++E ++ + +H+ VE+M+EKNI F H+ V++FVLS+D++S L ++ Sbjct: 183 ADGKSLLIASAVEALSKKHNANIHSVVEVMEEKNIPLFNHIDVDDFVLSNDSVSLLMAKA 242 Query: 253 ALQEGNSEVITQLLSREHGDDIYEIPRDAAWHTYGDAFQALLRRGATLLSDRGDLGINRK 312 LQ G + + QLLS++ G +I+E A W +Y DA Q L RGA L++ D+ + Sbjct: 243 TLQPGTTGLFRQLLSKKFGKNIHEFTPLADWKSYKDASQFLFDRGAVLIAVNEDMDFSNA 302 Query: 313 LDQPIPQDARLYIVSDEETYRQI 335 ++ + +D LYI+ D++ Y+ + Sbjct: 303 AEKKLDKDDTLYIICDDQIYQDL 325 >C4KZ24.1 C4KZ24_EXISA Ion transport 2 domain protein Length = 339 Score = 249 bits (636), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 127/339 (37%), Positives = 209/339 (61%), Gaps = 5/339 (1%) Query: 1 MHFLLRLSGKLMRLKKKSIGLIILAFIVISATIAFLLEPGTFSNWFNAFYWVMTTMSTVG 60 MH L R+ L +L ++ L + I++ + ++LEP TF + F++F+W MTT++TVG Sbjct: 1 MHILFRVWKGLAKLSFLNVVLASIGIIILGTVLGYVLEPETFGSLFDSFWWTMTTLTTVG 60 Query: 61 YGDFFAGTVAGKLFTIFLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLI 120 YGDF+ +VAG+ IFL++FGIG++ +IGK+++ QR + G L + G+ H V I Sbjct: 61 YGDFYPSSVAGRWLGIFLFLFGIGIIGALIGKLVEIGATFQRLKREGRLVYRGEGHYVYI 120 Query: 121 NWNRKTQAAVDEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGA 180 W+ KT+ A+DE+L Y P IV+ID+ + P + VHF+SGD S + +LL+A + A Sbjct: 121 GWSPKTKKAIDEVLNYEPKAEIVLIDQLVEEPYVH-DQVHFVSGDASDEAVLLQANVLKA 179 Query: 181 KAAIVFGDTRIDEASLTDGKTLLIASSIERIAPQ----VHTTVEIMQEKNIQNFRHVHVN 236 + +F D RI E L DGK+LLIAS++E ++ + +HT VE+ +E+NI F+HV V+ Sbjct: 180 RRVAIFADARITETLLADGKSLLIASAVEALSSEYQVDLHTVVEVCEERNISKFKHVRVD 239 Query: 237 EFVLSHDAISRLAVRSALQEGNSEVITQLLSREHGDDIYEIPRDAAWHTYGDAFQALLRR 296 +F+L++D++S L ++ LQ G + + QLLS++ G +I + W A ALL Sbjct: 240 DFILANDSVSLLMAKATLQPGTTAIFRQLLSKQSGGNIQALKPKVEWLNVRQANTALLES 299 Query: 297 GATLLSDRGDLGINRKLDQPIPQDARLYIVSDEETYRQI 335 G TL++ L + LD P+ + LY+V ++ + ++ Sbjct: 300 GVTLIAVNDRLDVAMMLDLPLQETDMLYVVCHDDVFERL 338 >B1YEW8.1 B1YEW8_EXIS2 Ion transport 2 domain protein Length = 339 Score = 244 bits (622), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 125/331 (37%), Positives = 203/331 (61%), Gaps = 6/331 (1%) Query: 5 LRLSGKLMRLKKKSIGLIILAFIVISATIAFLLEPGTFSNWFNAFYWVMTTMSTVGYGDF 64 LR ++ L+ +++ F+ A I +L EP TF +F A YW MTT+ TVGYGDF Sbjct: 12 LRFLRRMSFLRMRTLAYASFLFVFGVALIMYLAEPDTFGTYFRAVYWTMTTVVTVGYGDF 71 Query: 65 FAGTVAGKLFTIFLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLINWNR 124 F T G+ TIF++IFGIG++ +I K++D + ++ ++ G L H ++ ++R Sbjct: 72 FPNTDFGRFMTIFVFIFGIGIVGGLISKLVDGVQLIRNQKERGLLQVKETGHTLVFGYSR 131 Query: 125 KTQAAVDEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGAKAAI 184 +++ +DE+L T +++ID+ + P H++SGDP+ D L +A I+ A AI Sbjct: 132 RSKHVIDELLETT---DVILIDDLEREPFSH-PRFHYVSGDPALDATLQRANIKKATRAI 187 Query: 185 VFGDTRIDEASLTDGKTLLIASSIERIAPQVHTTVEIMQEKNIQNFRHVHVNEFVLSHDA 244 V D I A L DG+TLLIA+SIER P ++T VE+M E+++ +F V V+E +L + Sbjct: 188 VLADHSIPPA-LADGRTLLIAASIERANPNIYTIVEMMLEEHLDSFEQVKVDEVLLGDET 246 Query: 245 ISRLAVRSALQEGNSEVITQLLSREHGDDIYEIPRDAAWHTYGDAFQALLRRGATLLSDR 304 ++R+A+ +A G S+V+T LL+++ GD ++ +P W T+ DAF LL G T+LSD Sbjct: 247 VARMAILAAYHPGVSKVVTNLLTKD-GDGMFSLPAKPEWQTFRDAFHILLEDGVTILSDG 305 Query: 305 GDLGINRKLDQPIPQDARLYIVSDEETYRQI 335 L +NR+L++PIP + L ++ EET ++I Sbjct: 306 KRLDLNRRLNEPIPPNTTLIVLCSEETAQRI 336 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,964,231,078 Number of extensions: 82384378 Number of successful extensions: 272495 Number of sequences better than 1.0e-05: 703 Number of HSP's gapped: 272309 Number of HSP's successfully gapped: 703 Length of query: 337 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 201 Effective length of database: 1,779,438,163 Effective search space: 357667070763 Effective search space used: 357667070763 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)