BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0005.p (512 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DA53.1 B1DA53_9BACL Transmembrane protein 512 e-143 Q8Y9K0.1 Q8Y9K0_LISMO Transmembrane protein 462 e-128 Q4EVT0.1 Q4EVT0_LISMO Membrane protein, putative 462 e-128 B8DA99.1 B8DA99_LISMH Transmembrane protein 461 e-128 Q4EIG7.1 Q4EIG7_LISMO Membrane protein, putative 461 e-127 >B1DA53.1 B1DA53_9BACL Transmembrane protein Length = 511 Score = 512 bits (1318), Expect = e-143, Method: Compositional matrix adjust. Identities = 263/504 (52%), Positives = 358/504 (71%), Gaps = 6/504 (1%) Query: 4 DNLVKTTLRQRIMPAGLRVSPAFAAAFGVLLVTVIALFTAPYIGMADNGDFFRNIYSNGL 63 + L + L Q I+ +SPA AAFGV L+ +I LF PYIGMADNGD++R +YSNGL Sbjct: 2 NKLQRMKLVQTIIIWQKYISPASLAAFGVALILLIELFIPPYIGMADNGDYYRILYSNGL 61 Query: 64 YFNLPDYDSKYFGYFVKHFGIYQYYNENGATLDSSQSLFIKLSMGLNKLVFSSTVFDIRF 123 YFN PDY S+ GYFVK+FGI+QYYNEN A L SSQ+LF++LS+ +++ FDIR Sbjct: 62 YFNAPDYTSQTLGYFVKNFGIFQYYNENSALLYSSQTLFVRLSLMISEWFGYGRQFDIRI 121 Query: 124 QAAIYTVLYVAAIYLLIEAVTCKMSRKQGMIIAVIAILVFGDTGYTAYFNSFYSESVVMI 183 QAA+Y+VLYV A+YLL++A T K SR G +A+ AI VFGD GY AYFNSFYSESVV+I Sbjct: 122 QAALYSVLYVGAVYLLVKAATWKASRLAGYGLALGAIFVFGDIGYLAYFNSFYSESVVLI 181 Query: 184 MMIFVFASWLLLYRKRYNDYALLGLFVLSSIILTTSKQQNAPVGMIIAVLGISLVWIRRD 243 M++++F++ +LLY+++YNDY LL LF S++ILTTSKQQNAP+G+I+A + + V+++R+ Sbjct: 182 MLMYLFSAGMLLYQRQYNDYFLLFLFGASALILTTSKQQNAPIGLIVAAVAVLFVFLKRN 241 Query: 244 RLFRSLTLVSMALLMLAGIATYLNISKEFVNINQYHAMTRGVLKESSNPETTLKSFGIDE 303 R+FR+L + +L+L + TY+ I ++FVNIN+YHAMTRGVL + NPE L F ID Sbjct: 242 RIFRTLAGMQAVILILTAVGTYVLIPEQFVNINKYHAMTRGVLMTADNPEEALDFFEIDR 301 Query: 304 QYAILKGSIYYEQYGTVDVNSPMLEKDFYSRYGFGSILKYYATNPGQLGSILNVAARSAF 363 Q+A+L G+IYY+Q+ + V S ++K F +YGF SI +YY T+P G I AAR+AF Sbjct: 302 QFAVLTGAIYYDQFSPIPVESEEMQKQFLDKYGFPSIARYYLTHPDSAGRIFETAARNAF 361 Query: 364 SIKPAAMGNYEESEGKAFRAQSHFFTAYSLLKQKLAPKTFGFILLWMALVIGLYMPSCVK 423 S + +GNYE+S K F ++ F+ YS LK+ APKT GF++LWM ++ GLY S Sbjct: 362 SNR-TKLGNYEKSADKPFGKRTLAFSGYSYLKEWAAPKTSGFVVLWMIVIAGLYAASFYH 420 Query: 424 AVKARNMRAFQRMVLILASMGVGLSGIFVSIIGAGDADISKHEFLFTLCFDLVTFMLAAD 483 AVK R +R R+ +IL M GL + VS+IGAGDAD++KHEFLF + FDLVTF + AD Sbjct: 421 AVKERALRRTLRLSMILMMMLSGLCAMAVSVIGAGDADLAKHEFLFAVAFDLVTFWVIAD 480 Query: 484 VIGRRVSAGRHPAARK-AGQY-PE 505 + RR+ P A+K AG+ PE Sbjct: 481 CVCRRLWG---PNAKKNAGEEDPE 501 >Q8Y9K0.1 Q8Y9K0_LISMO Transmembrane protein Length = 491 Score = 462 bits (1188), Expect = e-128, Method: Compositional matrix adjust. Identities = 216/465 (46%), Positives = 327/465 (70%) Query: 21 RVSPAFAAAFGVLLVTVIALFTAPYIGMADNGDFFRNIYSNGLYFNLPDYDSKYFGYFVK 80 ++P A V ++T+IALF PYIGMADNGDFFR SNGL+ N +YD+ FG+FVK Sbjct: 15 HLTPQIFAVICVFIITIIALFMPPYIGMADNGDFFRIFSSNGLFVNNTNYDALQFGHFVK 74 Query: 81 HFGIYQYYNENGATLDSSQSLFIKLSMGLNKLVFSSTVFDIRFQAAIYTVLYVAAIYLLI 140 FGIYQY+NEN + SSQS+FI++++ LNKL +S+TVFD+RF + L + AIYLL+ Sbjct: 75 EFGIYQYFNENQVAIYSSQSIFIQMALLLNKLFWSTTVFDVRFLGGLQLALLLPAIYLLV 134 Query: 141 EAVTCKMSRKQGMIIAVIAILVFGDTGYTAYFNSFYSESVVMIMMIFVFASWLLLYRKRY 200 +T KM G ++A + + +F DT YTAYFNSF+SE +++IMM+++ A +LLLY+ +Y Sbjct: 135 AGLTAKMKGWPGYVVAALTVFIFADTAYTAYFNSFFSEGLILIMMLYISAGFLLLYQHKY 194 Query: 201 NDYALLGLFVLSSIILTTSKQQNAPVGMIIAVLGISLVWIRRDRLFRSLTLVSMALLMLA 260 NDYA+LGL ++S+IL T+KQQNAP+ ++IAV GI + +IR++R FR + ++ L+ Sbjct: 195 NDYAMLGLIFVASLILITAKQQNAPIAVVIAVCGILVFFIRKNRAFRISAAATFLVIFLS 254 Query: 261 GIATYLNISKEFVNINQYHAMTRGVLKESSNPETTLKSFGIDEQYAILKGSIYYEQYGTV 320 G+ Y I EFV INQY MTRGVL +S NPE +L+ G++ ++A+LKG+ +Y++Y + Sbjct: 255 GVVMYAFIPGEFVTINQYQTMTRGVLLDSENPEKSLEEMGMNSEFALLKGTNFYQKYKMI 314 Query: 321 DVNSPMLEKDFYSRYGFGSILKYYATNPGQLGSILNVAARSAFSIKPAAMGNYEESEGKA 380 D++S ++EK+FY Y F ++L YY NP Q G +L+++A+++FSI+P MGN+E++ G Sbjct: 315 DLDSKLMEKEFYPNYNFVTVLSYYLENPKQFGKMLDLSAQNSFSIRPFEMGNFEKATGYK 374 Query: 381 FRAQSHFFTAYSLLKQKLAPKTFGFILLWMALVIGLYMPSCVKAVKARNMRAFQRMVLIL 440 F+ ++HFF+AYS +K+K AP F F++LW + + Y S K + +N+R LI+ Sbjct: 375 FKEKTHFFSAYSDVKEKFAPAKFTFLILWALVFLICYGVSAFKHFREKNIRGTLLFDLIV 434 Query: 441 ASMGVGLSGIFVSIIGAGDADISKHEFLFTLCFDLVTFMLAADVI 485 +G G + I V+I+G G+AD++KH FLF +CFDL + AA ++ Sbjct: 435 LLIGSGFAVILVTIVGDGEADLTKHNFLFNVCFDLTMLIGAASLL 479 >Q4EVT0.1 Q4EVT0_LISMO Membrane protein, putative Length = 491 Score = 462 bits (1188), Expect = e-128, Method: Compositional matrix adjust. Identities = 216/465 (46%), Positives = 327/465 (70%) Query: 21 RVSPAFAAAFGVLLVTVIALFTAPYIGMADNGDFFRNIYSNGLYFNLPDYDSKYFGYFVK 80 ++P A V ++T+IALF PYIGMADNGDFFR SNGL+ N +YD+ FG+FVK Sbjct: 15 HLTPQIFAVICVFIITIIALFMPPYIGMADNGDFFRIFSSNGLFVNNTNYDALQFGHFVK 74 Query: 81 HFGIYQYYNENGATLDSSQSLFIKLSMGLNKLVFSSTVFDIRFQAAIYTVLYVAAIYLLI 140 FGIYQY+NEN + SSQS+FI++++ LNKL +S+TVFD+RF + L + AIYLL+ Sbjct: 75 EFGIYQYFNENQVAIYSSQSIFIQMALLLNKLFWSTTVFDVRFLGGLQLALLLPAIYLLV 134 Query: 141 EAVTCKMSRKQGMIIAVIAILVFGDTGYTAYFNSFYSESVVMIMMIFVFASWLLLYRKRY 200 +T KM G ++A + + +F DT YTAYFNSF+SE +++IMM+++ A +LLLY+ +Y Sbjct: 135 AGLTAKMKGWPGYVVAALTVFIFADTAYTAYFNSFFSEGLILIMMLYISAGFLLLYQHKY 194 Query: 201 NDYALLGLFVLSSIILTTSKQQNAPVGMIIAVLGISLVWIRRDRLFRSLTLVSMALLMLA 260 NDYA+LGL ++S+IL T+KQQNAP+ ++IAV GI + +IR++R FR + ++ L+ Sbjct: 195 NDYAMLGLIFVASLILITAKQQNAPIAVVIAVCGILVFFIRKNRAFRISAAATFLVIFLS 254 Query: 261 GIATYLNISKEFVNINQYHAMTRGVLKESSNPETTLKSFGIDEQYAILKGSIYYEQYGTV 320 G+ Y I EFV INQY MTRGVL +S NPE +L+ G++ ++A+LKG+ +Y++Y + Sbjct: 255 GVVMYAFIPGEFVTINQYQTMTRGVLLDSENPEKSLEEMGMNSEFALLKGTNFYQKYKMI 314 Query: 321 DVNSPMLEKDFYSRYGFGSILKYYATNPGQLGSILNVAARSAFSIKPAAMGNYEESEGKA 380 D++S ++EK+FY Y F ++L YY NP Q G +L+++A+++FSI+P MGN+E++ G Sbjct: 315 DLDSKLMEKEFYPNYNFVTVLSYYLENPKQFGKMLDLSAQNSFSIRPFEMGNFEKATGYK 374 Query: 381 FRAQSHFFTAYSLLKQKLAPKTFGFILLWMALVIGLYMPSCVKAVKARNMRAFQRMVLIL 440 F+ ++HFF+AYS +K+K AP F F++LW + + Y S K + +N+R LI+ Sbjct: 375 FKEKTHFFSAYSDVKEKFAPAKFTFLILWALVFLICYGVSAFKHFREKNIRGTLLFDLIV 434 Query: 441 ASMGVGLSGIFVSIIGAGDADISKHEFLFTLCFDLVTFMLAADVI 485 +G G + I V+I+G G+AD++KH FLF +CFDL + AA ++ Sbjct: 435 LLIGSGFAVILVTIVGDGEADLTKHNFLFNVCFDLTMLIGAASLL 479 >B8DA99.1 B8DA99_LISMH Transmembrane protein Length = 491 Score = 461 bits (1186), Expect = e-128, Method: Compositional matrix adjust. Identities = 217/479 (45%), Positives = 332/479 (69%) Query: 7 VKTTLRQRIMPAGLRVSPAFAAAFGVLLVTVIALFTAPYIGMADNGDFFRNIYSNGLYFN 66 +K ++ M ++P A V ++T+IALF PYIGMADNGDFFR SNGL+ N Sbjct: 1 MKGKIKSSSMWLWHHLTPQIFAVICVFIITLIALFVPPYIGMADNGDFFRIFSSNGLFVN 60 Query: 67 LPDYDSKYFGYFVKHFGIYQYYNENGATLDSSQSLFIKLSMGLNKLVFSSTVFDIRFQAA 126 +YD+ FG+FVK FGIYQY+NEN + SSQS+FI++++ LNKL +S+TVFD+RF Sbjct: 61 NTNYDALQFGHFVKEFGIYQYFNENQVAIYSSQSIFIQMALLLNKLFWSTTVFDVRFLGG 120 Query: 127 IYTVLYVAAIYLLIEAVTCKMSRKQGMIIAVIAILVFGDTGYTAYFNSFYSESVVMIMMI 186 + L + AIYLL+ +T KM G ++A + + +F DT YTAYFNSF+SE +++IMM+ Sbjct: 121 LQLALLLPAIYLLVAGLTAKMKGWPGYVVAALTVFIFADTAYTAYFNSFFSEGLILIMML 180 Query: 187 FVFASWLLLYRKRYNDYALLGLFVLSSIILTTSKQQNAPVGMIIAVLGISLVWIRRDRLF 246 ++ A +LLLY+ +YNDYA+LGL ++S+IL T+KQQNAP+ ++IAV GI + +IR++R F Sbjct: 181 YISAGFLLLYQHKYNDYAMLGLIFVASLILITAKQQNAPIAVVIAVCGILVFFIRKNRAF 240 Query: 247 RSLTLVSMALLMLAGIATYLNISKEFVNINQYHAMTRGVLKESSNPETTLKSFGIDEQYA 306 R + ++ L+G+ Y I EFV INQY MTRGVL +S NPE +L+ G++ ++A Sbjct: 241 RISAAATFFVIFLSGVVMYAFIPGEFVTINQYQTMTRGVLLDSENPEKSLEEMGMNSEFA 300 Query: 307 ILKGSIYYEQYGTVDVNSPMLEKDFYSRYGFGSILKYYATNPGQLGSILNVAARSAFSIK 366 +LKG+ +Y++Y +D++S ++EK+FY Y F ++L YY NP Q G +L+++A+++FSI+ Sbjct: 301 LLKGTNFYQKYKMIDLDSKLMEKEFYPNYNFVTVLSYYLENPKQFGKMLDLSAQNSFSIR 360 Query: 367 PAAMGNYEESEGKAFRAQSHFFTAYSLLKQKLAPKTFGFILLWMALVIGLYMPSCVKAVK 426 P MGN+E++ G F+ ++HFF+ YS +K+KLAP F F++LW + + Y S K + Sbjct: 361 PFEMGNFEKATGYKFKEKTHFFSIYSDVKEKLAPAKFTFLILWALVFLICYGVSAFKHFR 420 Query: 427 ARNMRAFQRMVLILASMGVGLSGIFVSIIGAGDADISKHEFLFTLCFDLVTFMLAADVI 485 +N+R L++ +G G + I V+I+G G+AD++KH FLF +CFDL + AA ++ Sbjct: 421 EKNIRGMLLFDLVVLLIGSGFAVILVTIVGDGEADLTKHNFLFNVCFDLTMLIGAASLL 479 >Q4EIG7.1 Q4EIG7_LISMO Membrane protein, putative Length = 491 Score = 461 bits (1185), Expect = e-127, Method: Compositional matrix adjust. Identities = 215/465 (46%), Positives = 327/465 (70%) Query: 21 RVSPAFAAAFGVLLVTVIALFTAPYIGMADNGDFFRNIYSNGLYFNLPDYDSKYFGYFVK 80 ++P A V ++T+IALF PYIGMADNGDFFR SNGL+ N +YD+ FG+FVK Sbjct: 15 HLTPQIFAVICVFIITLIALFVPPYIGMADNGDFFRIFSSNGLFVNNTNYDALQFGHFVK 74 Query: 81 HFGIYQYYNENGATLDSSQSLFIKLSMGLNKLVFSSTVFDIRFQAAIYTVLYVAAIYLLI 140 FGIYQY+NEN + SSQS+FI++++ LNKL +S+TVFD+RF + L + AIYLL+ Sbjct: 75 EFGIYQYFNENQVAIYSSQSIFIQMALLLNKLFWSTTVFDVRFLGGLQLALLLPAIYLLV 134 Query: 141 EAVTCKMSRKQGMIIAVIAILVFGDTGYTAYFNSFYSESVVMIMMIFVFASWLLLYRKRY 200 +T KM G ++A + + +F DT YTAYFNSF+SE +++IMM+++ A +LLLY+ +Y Sbjct: 135 AGLTAKMKGWPGYVVAALTVFIFADTAYTAYFNSFFSEGLILIMMLYISAGFLLLYQHKY 194 Query: 201 NDYALLGLFVLSSIILTTSKQQNAPVGMIIAVLGISLVWIRRDRLFRSLTLVSMALLMLA 260 NDYA+LGL ++S+IL T+KQQNAP+ ++IAV GI + +IR++R FR + ++ L+ Sbjct: 195 NDYAMLGLIFVASLILITAKQQNAPIAVVIAVCGILVFFIRKNRAFRISAAATFFVIFLS 254 Query: 261 GIATYLNISKEFVNINQYHAMTRGVLKESSNPETTLKSFGIDEQYAILKGSIYYEQYGTV 320 G+ Y I EFV INQY MTRGVL +S NPE +L+ G++ ++A+LKG+ +Y++Y + Sbjct: 255 GVVMYAFIPGEFVTINQYQTMTRGVLLDSENPEKSLEEMGMNSEFALLKGTNFYQKYKMI 314 Query: 321 DVNSPMLEKDFYSRYGFGSILKYYATNPGQLGSILNVAARSAFSIKPAAMGNYEESEGKA 380 D++S ++EK+FY Y F ++L YY NP Q G +L+++A+++FSI+P MGN+E++ G Sbjct: 315 DLDSKLMEKEFYPNYNFVTVLSYYLENPKQFGKMLDLSAQNSFSIRPFEMGNFEKATGYK 374 Query: 381 FRAQSHFFTAYSLLKQKLAPKTFGFILLWMALVIGLYMPSCVKAVKARNMRAFQRMVLIL 440 F+ ++HFF+ YS +K+KLAP F F++LW + + Y S K + +N+R L++ Sbjct: 375 FKEKTHFFSIYSDVKEKLAPAKFTFLILWALVFLICYGVSAFKHFREKNIRGMLLFDLVI 434 Query: 441 ASMGVGLSGIFVSIIGAGDADISKHEFLFTLCFDLVTFMLAADVI 485 +G G + I V+I+G G+AD++KH FLF +CFDL + AA ++ Sbjct: 435 LLIGSGFAVILVTIVGDGEADLTKHNFLFNVCFDLTMLIGAASLL 479 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.139 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,838,578,077 Number of extensions: 116248638 Number of successful extensions: 377566 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 377437 Number of HSP's successfully gapped: 42 Length of query: 512 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 372 Effective length of database: 1,741,754,103 Effective search space: 647932526316 Effective search space used: 647932526316 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 134 (56.2 bits)