BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0075.p (360 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB26.1 B3KB26_9BACI 3-isopropylmalate dehydrogenase EC=1.1.1.85 625 e-177 B1DL44.1 B1DL44_9BACL 3-isopropylmalate dehydrogenase EC=1.1.1.85 618 e-175 C0Z9C9.1 C0Z9C9_BREBN 3-isopropylmalate dehydrogenase EC=1.1.1.85 471 e-131 A3DHI2.1 A3DHI2_CLOTH 3-isopropylmalate dehydrogenase EC=1.1.1.85 434 e-120 B4BC97.1 B4BC97_CLOTM 3-isopropylmalate dehydrogenase EC=1.1.1.85 434 e-120 >B3KB26.1 B3KB26_9BACI 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 358 Score = 625 bits (1613), Expect = e-177, Method: Compositional matrix adjust. Identities = 304/358 (84%), Positives = 324/358 (90%) Query: 1 MSEVKKIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPED 60 M+E KKIAVIAGDGIGPEVVAEA K++K+TEE+FG +F TE ALFGGIAIDE+GTPLPE+ Sbjct: 1 MAETKKIAVIAGDGIGPEVVAEAEKIIKRTEEVFGLSFTTEHALFGGIAIDEKGTPLPEE 60 Query: 61 TLEICRSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDAS 120 TL +C+SADAVLLGAVGGPKWDNN KELRPETGLLGIRK LGLFSNLRPAV+FDCLKDAS Sbjct: 61 TLSVCKSADAVLLGAVGGPKWDNNAKELRPETGLLGIRKELGLFSNLRPAVIFDCLKDAS 120 Query: 121 TLKPEVLEGTDLMVVRELTGGIYFGDKLRRQGEFGEEAVDTCVYNVTEVERIVRQAFEIA 180 TLKPEVLEGTDLMVVRELTGGIYFG+K RR+G GEEAVDTCVYN EVERIV QAFEIA Sbjct: 121 TLKPEVLEGTDLMVVRELTGGIYFGEKFRREGAGGEEAVDTCVYNTAEVERIVHQAFEIA 180 Query: 181 GKRRNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVI 240 KRR KLASVDKANVLETSRLWRE VNRIAPEYP VELEHVLVDNCAMQLLRRPSSFDVI Sbjct: 181 QKRRKKLASVDKANVLETSRLWRETVNRIAPEYPDVELEHVLVDNCAMQLLRRPSSFDVI 240 Query: 241 VTENMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATIL 300 VTENMFGDILSDEAAMLTGSIGMLASAS+GEG++GLYEPVHGSAPDIAGQGLANPIATIL Sbjct: 241 VTENMFGDILSDEAAMLTGSIGMLASASMGEGAFGLYEPVHGSAPDIAGQGLANPIATIL 300 Query: 301 SLALMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLIVAAIR 358 S+ALM+RLTFGY LDAGHRT+DIAVDKSKALST +MGDLI AAIR Sbjct: 301 SVALMYRLTFGYEDAADAIERAVAEVLDAGHRTADIAVDKSKALSTTEMGDLIAAAIR 358 >B1DL44.1 B1DL44_9BACL 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 359 Score = 618 bits (1594), Expect = e-175, Method: Compositional matrix adjust. Identities = 302/359 (84%), Positives = 323/359 (89%) Query: 1 MSEVKKIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPED 60 MSEVKKIAVIAGDGIGPEVV EALKV+KKTEELFGY FE E LFGGIAIDE+GTPLP++ Sbjct: 1 MSEVKKIAVIAGDGIGPEVVGEALKVMKKTEELFGYQFEFEHGLFGGIAIDEKGTPLPQE 60 Query: 61 TLEICRSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDAS 120 TL+IC+ ADAVLLGAVGGPKWDNN KELRPETGLLGIRKALGLFSN+RPA VFDCLK+AS Sbjct: 61 TLDICKKADAVLLGAVGGPKWDNNSKELRPETGLLGIRKALGLFSNIRPANVFDCLKEAS 120 Query: 121 TLKPEVLEGTDLMVVRELTGGIYFGDKLRRQGEFGEEAVDTCVYNVTEVERIVRQAFEIA 180 TLKPEVLEGTDL+VVRELTGGIYFG+K RR+G GEEAVDTCVYNV EVERIVRQAF+IA Sbjct: 121 TLKPEVLEGTDLIVVRELTGGIYFGEKFRREGASGEEAVDTCVYNVQEVERIVRQAFDIA 180 Query: 181 GKRRNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVI 240 R +LASVDKANVLETSRLWREVVNRIAPEYP+VELEHVLVDNCAMQLLRRPSSFDVI Sbjct: 181 MTRSKRLASVDKANVLETSRLWREVVNRIAPEYPEVELEHVLVDNCAMQLLRRPSSFDVI 240 Query: 241 VTENMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATIL 300 VTENMFGDILSDEAAMLTGSIGML+SASLGEGS+GLYEPVHGSAPDIAGQG++NPIATIL Sbjct: 241 VTENMFGDILSDEAAMLTGSIGMLSSASLGEGSFGLYEPVHGSAPDIAGQGISNPIATIL 300 Query: 301 SLALMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLIVAAIRK 359 S+ALMFRLTFGY LDAGHRT DIAVDKS A+ T MGDLIVAA+ K Sbjct: 301 SVALMFRLTFGYHEAAQVIEDAVKNVLDAGHRTGDIAVDKSTAIGTTAMGDLIVAAMTK 359 >C0Z9C9.1 C0Z9C9_BREBN 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 364 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 230/353 (65%), Positives = 273/353 (77%) Query: 1 MSEVKKIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPED 60 M + +IAV+ GDGIGPE++ EA+KVL EL G F E+ GGIAID GTPLPE+ Sbjct: 1 MKKNYRIAVLPGDGIGPEIMQEAVKVLTLVGELEGVTFTCEEGRIGGIAIDTDGTPLPEE 60 Query: 61 TLEICRSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDAS 120 T+ + + ADAVLLGAVGGPKWD NP LRPETGLLGIRKALGLF+N+RPA + L DAS Sbjct: 61 TVTLAKQADAVLLGAVGGPKWDQNPGHLRPETGLLGIRKALGLFANIRPATMHSSLVDAS 120 Query: 121 TLKPEVLEGTDLMVVRELTGGIYFGDKLRRQGEFGEEAVDTCVYNVTEVERIVRQAFEIA 180 TLKPEV+ G DL+VVRELTGGIYFG+K R G GE A D C+Y+ EVERI+R F+IA Sbjct: 121 TLKPEVVSGVDLIVVRELTGGIYFGEKKRYDGPNGEVAEDQCIYHEAEVERIIRVGFDIA 180 Query: 181 GKRRNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVI 240 KR+ +L SVDKANVLE+SRLWR+V R+A +YP VEL H LVD+CAMQL+R P FDVI Sbjct: 181 RKRQKRLVSVDKANVLESSRLWRKVAERVAADYPDVELSHQLVDSCAMQLVREPKQFDVI 240 Query: 241 VTENMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATIL 300 VTENMFGDILSD+AAMLTGSIGML+SASL GS+GLYEPVHGSAPDIAG+G+ANP+ATIL Sbjct: 241 VTENMFGDILSDQAAMLTGSIGMLSSASLAAGSFGLYEPVHGSAPDIAGKGIANPLATIL 300 Query: 301 SLALMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLI 353 S+A+M RL+ G L+AGHRT DIA D+SKA+ T +MG L+ Sbjct: 301 SVAMMLRLSLGMDEAAAAVENAVWSVLEAGHRTGDIAADRSKAIGTIEMGQLV 353 >A3DHI2.1 A3DHI2_CLOTH 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 362 Score = 434 bits (1116), Expect = e-120, Method: Compositional matrix adjust. Identities = 216/358 (60%), Positives = 272/358 (75%), Gaps = 5/358 (1%) Query: 6 KIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEIC 65 KIAV+ GDGIGPEV+ +A+KV++ E++ ++FE ++ L GG AID G P P++TLE+C Sbjct: 5 KIAVLPGDGIGPEVIEQAVKVIEAVGEIYNHSFELKEGLLGGCAIDATGEPFPKETLELC 64 Query: 66 RSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKPE 125 +S+DAVLLGAVGGPKWDN P + RPE GLLGIR ALGL++NLRPAV++ LK AS L+ + Sbjct: 65 KSSDAVLLGAVGGPKWDNLPGDKRPEAGLLGIRGALGLYANLRPAVIYQALKGASPLRSD 124 Query: 126 VL-EGTDLMVVRELTGGIYFGDKLRRQGE-FGEEAVDTCVYNVTEVERIVRQAFEIAGKR 183 ++ +G D+MVVRELTGG+YFG++ R Q E G+ A DT Y+ EVERI R AFE A KR Sbjct: 125 IVKDGIDIMVVRELTGGMYFGERGRVQTENMGQAAFDTEKYSEFEVERIARLAFETAMKR 184 Query: 184 RNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVIVTE 243 KL SVDKANVLE+SRLWREVVNR+A +YP+VEL H+ VDN AMQL+R P+ FDVIVT Sbjct: 185 NKKLTSVDKANVLESSRLWREVVNRVASDYPEVELNHMYVDNAAMQLVRNPAQFDVIVTS 244 Query: 244 NMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATILSLA 303 NMFGDILSDEA+M+TGSIGML SASLGEGS GLYEP+HGSAPDIAGQ ANPIATILS+A Sbjct: 245 NMFGDILSDEASMITGSIGMLPSASLGEGSLGLYEPIHGSAPDIAGQDKANPIATILSVA 304 Query: 304 LMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIA---VDKSKALSTAQMGDLIVAAIR 358 +M + +FG L G+RT+DIA + + T +MG LI++ ++ Sbjct: 305 MMMKYSFGLEDAFRAIENAVVNVLGMGYRTADIASPDTPRENIVGTKEMGRLIISKLK 362 >B4BC97.1 B4BC97_CLOTM 3-isopropylmalate dehydrogenase EC=1.1.1.85 Length = 362 Score = 434 bits (1116), Expect = e-120, Method: Compositional matrix adjust. Identities = 216/358 (60%), Positives = 272/358 (75%), Gaps = 5/358 (1%) Query: 6 KIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEIC 65 KIAV+ GDGIGPEV+ +A+KV++ E++ ++FE ++ L GG AID G P P++TLE+C Sbjct: 5 KIAVLPGDGIGPEVIEQAVKVIEAVGEIYNHSFELKEGLLGGCAIDATGEPFPKETLELC 64 Query: 66 RSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKPE 125 +S+DAVLLGAVGGPKWDN P + RPE GLLGIR ALGL++NLRPAV++ LK AS L+ + Sbjct: 65 KSSDAVLLGAVGGPKWDNLPGDKRPEAGLLGIRGALGLYANLRPAVIYQALKGASPLRSD 124 Query: 126 VL-EGTDLMVVRELTGGIYFGDKLRRQGE-FGEEAVDTCVYNVTEVERIVRQAFEIAGKR 183 ++ +G D+MVVRELTGG+YFG++ R Q E G+ A DT Y+ EVERI R AFE A KR Sbjct: 125 IVKDGIDIMVVRELTGGMYFGERGRVQTENMGQAAFDTEKYSEFEVERIARLAFETAMKR 184 Query: 184 RNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVIVTE 243 KL SVDKANVLE+SRLWREVVNR+A +YP+VEL H+ VDN AMQL+R P+ FDVIVT Sbjct: 185 NKKLTSVDKANVLESSRLWREVVNRVASDYPEVELNHMYVDNAAMQLVRNPAQFDVIVTS 244 Query: 244 NMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATILSLA 303 NMFGDILSDEA+M+TGSIGML SASLGEGS GLYEP+HGSAPDIAGQ ANPIATILS+A Sbjct: 245 NMFGDILSDEASMITGSIGMLPSASLGEGSLGLYEPIHGSAPDIAGQDKANPIATILSVA 304 Query: 304 LMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIA---VDKSKALSTAQMGDLIVAAIR 358 +M + +FG L G+RT+DIA + + T +MG LI++ ++ Sbjct: 305 MMMKYSFGLEDAFRAIENAVVNVLGMGYRTADIASPDTPRENIVGTKEMGRLIISKLK 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,977,170,644 Number of extensions: 81775286 Number of successful extensions: 207356 Number of sequences better than 1.0e-05: 3471 Number of HSP's gapped: 197526 Number of HSP's successfully gapped: 3477 Length of query: 360 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 223 Effective length of database: 1,770,017,148 Effective search space: 394713824004 Effective search space used: 394713824004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)