BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0067.p (513 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB23.1 B3KB23_9BACI 2-isopropylmalate synthase 843 0.0 B1DL46.1 B1DL46_9BACL 2-isopropylmalate synthase 771 0.0 C0Z9C8.1 LEU1_BREBN 2-isopropylmalate synthase EC=2.3.3.13 607 e-171 B7GH19.1 LEU1_ANOFW 2-isopropylmalate synthase EC=2.3.3.13 556 e-156 C5D5M0.1 C5D5M0_9BACI 2-isopropylmalate synthase 546 e-153 >B3KB23.1 B3KB23_9BACI 2-isopropylmalate synthase Length = 512 Score = 843 bits (2179), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/513 (80%), Positives = 454/513 (88%), Gaps = 1/513 (0%) Query: 1 LRKIYVFDTTLRDGEQSPGVNLNTREKVEIAYQLEKLGIDRMEAGFPAASPGDLXXXXXX 60 +RKIYVFDTTLRDGEQSPGVNLNTREKVEIA QLEKL IDRMEAGFPAASPGDL Sbjct: 1 MRKIYVFDTTLRDGEQSPGVNLNTREKVEIALQLEKLKIDRMEAGFPAASPGDLAAVNAV 60 Query: 61 XXXXXXXTLIGLSRSRESDIDAVREALQGAQDPCIHLFLATSPIHRQHKLRMEKAQVLET 120 T+IGLSRSRE+DIDAVREALQGAQDPCIH+FLATSPIHR+ KLRMEK QVLET Sbjct: 61 ARAVKDVTVIGLSRSRENDIDAVREALQGAQDPCIHVFLATSPIHRKFKLRMEKQQVLET 120 Query: 121 AQAAIRYAKKYFSKLEFSLEDAGRTERDFMAEMVAMAIREGANVVNIPDTVGYLNPSEYG 180 AQA+IRYAKKYFSK+EFSLEDAGRTE DFMAE+VAMA+REGANVVNIPDTVGYLNPSEYG Sbjct: 121 AQASIRYAKKYFSKVEFSLEDAGRTELDFMAEVVAMAVREGANVVNIPDTVGYLNPSEYG 180 Query: 181 AIFKFLKDTVPDIEKVQLSAHCHNDLGMATANTLAAIQNGADQIEGTINGIGERAGNTAI 240 AIFK+LK+ VPDIEKVQLSAHCHNDLGMATANTLAAI NGADQIEGTINGIGERAGNTAI Sbjct: 181 AIFKYLKENVPDIEKVQLSAHCHNDLGMATANTLAAILNGADQIEGTINGIGERAGNTAI 240 Query: 241 EEVALALETRSEFFDAKTSLVLSEISRTSRLVSRLTGMVVPGNKAIVGANAFAHESGIHQ 300 EE+A+ALETRSEFF AKT L L+EI+RTSRLVS+LTGMVVPGNKAIVGANAFAHESGIHQ Sbjct: 241 EEIAMALETRSEFFGAKTGLALTEIARTSRLVSKLTGMVVPGNKAIVGANAFAHESGIHQ 300 Query: 301 DGMLKEKTTYEIMTPETIGLKESKLVLGKHSGRHAFRDKLRDLGYDLLEEELNXXXXXXX 360 DGMLKEKTTYEIMTPE+IGLKESKLVLGKHSGRHAFR+KL DLGY+L EE++N Sbjct: 301 DGMLKEKTTYEIMTPESIGLKESKLVLGKHSGRHAFREKLLDLGYELTEEQVNDAFAKFK 360 Query: 361 XXXXXXXEVSDEDILALLEEKLLDTPEVFSLQTIYVTYGNEATPSATITIKGPEPQPIVA 420 EVSDED+LALLEEKL+DTPEVF L+TI+V YGNE+TP+A + I E Q I Sbjct: 361 VLADKKKEVSDEDLLALLEEKLIDTPEVFKLETIFVAYGNESTPTARVRI-ATEDQTIEE 419 Query: 421 VAEGNGSVDAIYNAIDQATREEVTLGDYSIKAVSRGKDAQGEVHVVLSQGEVAAQGRGLS 480 AEGNGSVDAIYNAID+ T EEVTL DYSIK+V+ GKDAQGEVHVVLSQ +VAAQGRGLS Sbjct: 420 EAEGNGSVDAIYNAIDKVTGEEVTLADYSIKSVTHGKDAQGEVHVVLSQNDVAAQGRGLS 479 Query: 481 TDILEASARAYLNALNKLLEKRKTYTKRDHANL 513 TDILEASARAY++A+N L+EKRKT+++R+ A L Sbjct: 480 TDILEASARAYVDAVNVLIEKRKTFSRRERATL 512 >B1DL46.1 B1DL46_9BACL 2-isopropylmalate synthase Length = 514 Score = 771 bits (1992), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/513 (73%), Positives = 436/513 (84%) Query: 1 LRKIYVFDTTLRDGEQSPGVNLNTREKVEIAYQLEKLGIDRMEAGFPAASPGDLXXXXXX 60 +RKIY+FDTTLRDGEQSPGVNLNT+EKVEIA QLEKLG+DR+EAGFPAASPGDL Sbjct: 1 MRKIYIFDTTLRDGEQSPGVNLNTKEKVEIALQLEKLGVDRIEAGFPAASPGDLAAVNAV 60 Query: 61 XXXXXXXTLIGLSRSRESDIDAVREALQGAQDPCIHLFLATSPIHRQHKLRMEKAQVLET 120 T+IGLSRSRE DIDAVREAL+GAQDP IH+FLATSPIHR+HKLRMEK QVLET Sbjct: 61 ASAVKNATIIGLSRSREQDIDAVREALKGAQDPSIHVFLATSPIHRKHKLRMEKEQVLET 120 Query: 121 AQAAIRYAKKYFSKLEFSLEDAGRTERDFMAEMVAMAIREGANVVNIPDTVGYLNPSEYG 180 A+ AI YAKKYFSK+EFSLEDAGRTE DF+ +M MAIR GA+VVN+PDTVGYLNP EYG Sbjct: 121 AERAITYAKKYFSKIEFSLEDAGRTELDFVCQMTDMAIRAGASVVNLPDTVGYLNPLEYG 180 Query: 181 AIFKFLKDTVPDIEKVQLSAHCHNDLGMATANTLAAIQNGADQIEGTINGIGERAGNTAI 240 IFK +K+ VP+IEK+QLSAHCH+DLGMATAN LAAI NGADQIEGTINGIGERAGNTA+ Sbjct: 181 NIFKTVKENVPNIEKIQLSAHCHDDLGMATANALAAILNGADQIEGTINGIGERAGNTAL 240 Query: 241 EEVALALETRSEFFDAKTSLVLSEISRTSRLVSRLTGMVVPGNKAIVGANAFAHESGIHQ 300 EEVALALETR ++F AKT L L EI+RTSRLVS+LTGMV+PGNKAIVGANAFAHESGIHQ Sbjct: 241 EEVALALETRFDYFGAKTGLNLQEIARTSRLVSKLTGMVIPGNKAIVGANAFAHESGIHQ 300 Query: 301 DGMLKEKTTYEIMTPETIGLKESKLVLGKHSGRHAFRDKLRDLGYDLLEEELNXXXXXXX 360 DGMLKEKTTYEI++PETIGL++SKLVLGKHSGRHAFR+KL DLGY+L EE +N Sbjct: 301 DGMLKEKTTYEIISPETIGLRDSKLVLGKHSGRHAFREKLIDLGYELGEEAVNAAFAKFK 360 Query: 361 XXXXXXXEVSDEDILALLEEKLLDTPEVFSLQTIYVTYGNEATPSATITIKGPEPQPIVA 420 V+DEDI A LEEKL+DTPEV++L+ I V+YGN++TPSA++ I+ + + Sbjct: 361 DLADRKKHVTDEDIRAFLEEKLVDTPEVYTLEAIQVSYGNQSTPSASVRIRTADSETREE 420 Query: 421 VAEGNGSVDAIYNAIDQATREEVTLGDYSIKAVSRGKDAQGEVHVVLSQGEVAAQGRGLS 480 VA GNGSVDAIYNAID+ T E V L DYSIK+VS+GKDAQGEVHVVL Q +++AQGRGLS Sbjct: 421 VAIGNGSVDAIYNAIDKVTAEAVELEDYSIKSVSQGKDAQGEVHVVLKQDDISAQGRGLS 480 Query: 481 TDILEASARAYLNALNKLLEKRKTYTKRDHANL 513 TDILEASARAY++ALN+L+EKRK+ +RD +L Sbjct: 481 TDILEASARAYIDALNRLIEKRKSPGRRDKMSL 513 >C0Z9C8.1 LEU1_BREBN 2-isopropylmalate synthase EC=2.3.3.13 Length = 512 Score = 607 bits (1564), Expect = e-171, Method: Compositional matrix adjust. Identities = 293/502 (58%), Positives = 378/502 (75%) Query: 1 LRKIYVFDTTLRDGEQSPGVNLNTREKVEIAYQLEKLGIDRMEAGFPAASPGDLXXXXXX 60 +R I +FDTTLRDGEQSPGVN++T EKVEIA QLEKLG++RMEAGF AASPGD Sbjct: 1 MRTIEIFDTTLRDGEQSPGVNISTNEKVEIALQLEKLGVNRMEAGFAAASPGDQKSVAEV 60 Query: 61 XXXXXXXTLIGLSRSRESDIDAVREALQGAQDPCIHLFLATSPIHRQHKLRMEKAQVLET 120 T++ L+R+ + D+D EAL+ AQ+ +H+FLATSPIHRQ KL M K +VL Sbjct: 61 AKRVKNATVVSLARAVKDDMDKAYEALRNAQNASLHVFLATSPIHRQFKLNMSKEEVLAR 120 Query: 121 AQAAIRYAKKYFSKLEFSLEDAGRTERDFMAEMVAMAIREGANVVNIPDTVGYLNPSEYG 180 A A+ YAKKYF++++FS EDA RTE DF+AE+V I+ GA VNIPDTVGY+ P +YG Sbjct: 121 AVEAVTYAKKYFTEVQFSAEDAARTEIDFLAEVVEAVIKAGATTVNIPDTVGYMTPYQYG 180 Query: 181 AIFKFLKDTVPDIEKVQLSAHCHNDLGMATANTLAAIQNGADQIEGTINGIGERAGNTAI 240 IF+ LK VP + ++LS HCH+DLGMA AN+LAA++ GA Q+EGTINGIGERAGN A+ Sbjct: 181 NIFRELKKRVPSTDLIRLSCHCHDDLGMAVANSLAAVEGGATQVEGTINGIGERAGNAAL 240 Query: 241 EEVALALETRSEFFDAKTSLVLSEISRTSRLVSRLTGMVVPGNKAIVGANAFAHESGIHQ 300 EEVALALETR +++ A T L L EI+RTS+LVSRLTGM+VPGNKA+VGANAFAHESGIHQ Sbjct: 241 EEVALALETRKDYYQATTKLNLKEIARTSQLVSRLTGMIVPGNKAVVGANAFAHESGIHQ 300 Query: 301 DGMLKEKTTYEIMTPETIGLKESKLVLGKHSGRHAFRDKLRDLGYDLLEEELNXXXXXXX 360 DG+LKE TTYEI+ PE++G K +KLVLGKHSGRHAF++KL DLGY L +EE+N Sbjct: 301 DGVLKEVTTYEIIRPESVGFKSNKLVLGKHSGRHAFKEKLVDLGYHLEQEEVNAAFAAFK 360 Query: 361 XXXXXXXEVSDEDILALLEEKLLDTPEVFSLQTIYVTYGNEATPSATITIKGPEPQPIVA 420 E++D+DILAL++ K++ PE F L+++ + YGN + P+A++ + + Sbjct: 361 VLCDKKKEITDDDILALVDSKMVRGPEAFQLESVQLAYGNISVPTASVRLVRADGSVCEE 420 Query: 421 VAEGNGSVDAIYNAIDQATREEVTLGDYSIKAVSRGKDAQGEVHVVLSQGEVAAQGRGLS 480 A GNGSVD+IY AID+AT EEV+L DY I +V+ G+DA GEV V L Q ++ GRG+S Sbjct: 421 AACGNGSVDSIYKAIDRATGEEVSLVDYKILSVTHGQDALGEVFVRLQQDDLIVTGRGVS 480 Query: 481 TDILEASARAYLNALNKLLEKR 502 TD+LEASA AY+ A+NK++E+R Sbjct: 481 TDVLEASAIAYVRAVNKIMERR 502 >B7GH19.1 LEU1_ANOFW 2-isopropylmalate synthase EC=2.3.3.13 Length = 514 Score = 556 bits (1432), Expect = e-156, Method: Compositional matrix adjust. Identities = 281/506 (55%), Positives = 370/506 (73%), Gaps = 3/506 (0%) Query: 1 LRKIYVFDTTLRDGEQSPGVNLNTREKVEIAYQLEKLGIDRMEAGFPAASPGDLXXXXXX 60 +RKI +FDTTLRDGEQS GVNLN EK+EIA QLE+LG+D +EAGFPAAS GD Sbjct: 1 MRKINIFDTTLRDGEQSAGVNLNLHEKLEIARQLERLGVDIIEAGFPAASKGDFQAVQAI 60 Query: 61 XXXXXXXTLIGLSRSRESDIDAVREALQGAQDPCIHLFLATSPIHRQHKLRMEKAQVLET 120 ++ GLSRS +SDIDA EAL+ +P +H+F+ATSPIH Q+KLRM QV+ET Sbjct: 61 AQTVRNCSVTGLSRSVQSDIDAAWEALKDGAEPRLHVFIATSPIHMQYKLRMTPEQVVET 120 Query: 121 AQAAIRYAKKYFSKLEFSLEDAGRTERDFMAEMVAMAIREGANVVNIPDTVGYLNPSEYG 180 A ++RYAKKYF +++S EDA R++ F+A +V I GANV+NIPDTVGY+ P +YG Sbjct: 121 AVESVRYAKKYFPIVQWSAEDACRSDLPFLATIVEKVIEAGANVINIPDTVGYITPKQYG 180 Query: 181 AIFKFLKDTVPDIEKVQLSAHCHNDLGMATANTLAAIQNGADQIEGTINGIGERAGNTAI 240 IF FLK V +IEK+ LSAHCH+DLGMA AN+LAAI+ GA QIEGTINGIGERAGN AI Sbjct: 181 DIFSFLKKNVRNIEKISLSAHCHDDLGMAVANSLAAIEAGATQIEGTINGIGERAGNAAI 240 Query: 241 EEVALALETRSEFFDAKTSLVLSEISRTSRLVSRLTGMVVPGNKAIVGANAFAHESGIHQ 300 EEVA+AL R +++ A+T L L EI RTS LVS+LTGM+VP NKAIVG NAFAHESGIHQ Sbjct: 241 EEVAVALYIRKDYYQAETRLNLQEIKRTSSLVSKLTGMIVPANKAIVGKNAFAHESGIHQ 300 Query: 301 DGMLKEKTTYEIMTPETIGLKESKLVLGKHSGRHAFRDKLRDLGYDLLEEELNXXXXXXX 360 DG+LKEKTTYEI++P+ +G++ + +VLGKHSGRHAFR ++++LGY L EEE+N Sbjct: 301 DGVLKEKTTYEIISPQLVGVQSNSMVLGKHSGRHAFRTRIQELGYSLTEEEVNRLFVRFK 360 Query: 361 XXXXXXXEVSDEDILALLEEKLLDTPEVF-SLQTIYVTYGNEATPSATITIKGPEPQPIV 419 +++D+D++AL+ ++ LDT + F L +I V YG P+A + +K E I Sbjct: 361 DLADKKKDITDDDLIALILDEKLDTYKNFYQLCSIQVQYGTNQIPTAVVVLKDGEGNDIQ 420 Query: 420 AVAEGNGSVDAIYNAIDQATREEVTLGDYSIKAVSRGKDAQGEVHVVLSQGEVAAQGRGL 479 A G GSV+A+YNA+++A + VTL DY I++V G+DA +V+V +S A GRG Sbjct: 421 EAATGAGSVEALYNALEKALQLPVTLLDYRIESVGSGRDALAQVYVKVSLDRKEASGRGT 480 Query: 480 STDILEASARAYLNALNKL--LEKRK 503 + D+LEASA+AY++A+N++ +EK K Sbjct: 481 AQDVLEASAKAYIHAVNRMFVIEKMK 506 >C5D5M0.1 C5D5M0_9BACI 2-isopropylmalate synthase Length = 515 Score = 546 bits (1406), Expect = e-153, Method: Compositional matrix adjust. Identities = 263/500 (52%), Positives = 366/500 (73%), Gaps = 1/500 (0%) Query: 1 LRKIYVFDTTLRDGEQSPGVNLNTREKVEIAYQLEKLGIDRMEAGFPAASPGDLXXXXXX 60 +RKI +FDTTLRDGEQS G+NLN +EK+EIA QLE+LG+D +EAGFPA+S GD Sbjct: 1 MRKINIFDTTLRDGEQSAGINLNLQEKLEIARQLERLGVDIIEAGFPASSKGDFQAVKQI 60 Query: 61 XXXXXXXTLIGLSRSRESDIDAVREALQGAQDPCIHLFLATSPIHRQHKLRMEKAQVLET 120 ++ GL+RS +SDIDA EAL+G +P +HLF+ATSPIH ++KL+M QV+ET Sbjct: 61 AETIKTCSVTGLARSVQSDIDAAWEALKGGAEPRLHLFIATSPIHMKYKLQMTPEQVIET 120 Query: 121 AQAAIRYAKKYFSKLEFSLEDAGRTERDFMAEMVAMAIREGANVVNIPDTVGYLNPSEYG 180 A +++YAK+YF +++S EDA R+E F+A+++ I+ GA V+NIPDTVGY+ P EYG Sbjct: 121 AVESVKYAKRYFPIVQWSAEDACRSELPFLAKIITEVIKAGATVINIPDTVGYITPKEYG 180 Query: 181 AIFKFLKDTVPDIEKVQLSAHCHNDLGMATANTLAAIQNGADQIEGTINGIGERAGNTAI 240 IF FL + VP+IEKV LSAHCH+DLGMA AN+LAAI++GA QIEGTINGIGERAGN A+ Sbjct: 181 NIFTFLSNNVPNIEKVSLSAHCHDDLGMAVANSLAAIEHGATQIEGTINGIGERAGNAAL 240 Query: 241 EEVALALETRSEFFDAKTSLVLSEISRTSRLVSRLTGMVVPGNKAIVGANAFAHESGIHQ 300 EE+A+AL R +++ A+T L L EI RTS LVS+LTG+V+P NKA++G NAFAHESGIHQ Sbjct: 241 EEIAVALYIRKDYYQAETRLNLQEIKRTSNLVSKLTGVVIPPNKAVIGKNAFAHESGIHQ 300 Query: 301 DGMLKEKTTYEIMTPETIGLKESKLVLGKHSGRHAFRDKLRDLGYDLLEEELNXXXXXXX 360 DG+LKEKTTYEI++PE +G++ + +VLGKHSGRHA R+++ +LGY L +EE+N Sbjct: 301 DGVLKEKTTYEIISPELVGVQSNSMVLGKHSGRHALRNRVEELGYTLSDEEVNKLFVRFK 360 Query: 361 XXXXXXXEVSDEDILALL-EEKLLDTPEVFSLQTIYVTYGNEATPSATITIKGPEPQPIV 419 +++D+D++AL+ EEK + + L ++ V YG P+A + +K + I Sbjct: 361 ELADKKKDITDDDLVALIFEEKFDHFKDFYQLSSLQVQYGTNQIPTAVVVLKDGQGNEIQ 420 Query: 420 AVAEGNGSVDAIYNAIDQATREEVTLGDYSIKAVSRGKDAQGEVHVVLSQGEVAAQGRGL 479 A G GSV+A+YN +++ + VTL DY I++VS G+DA +V V + ++ GRG Sbjct: 421 EAATGAGSVEALYNTLERCFKTSVTLLDYRIESVSGGRDALAQVFVKVRVNDIETSGRGT 480 Query: 480 STDILEASARAYLNALNKLL 499 + D+LEASA+AY+NA+N++ Sbjct: 481 AQDVLEASAKAYINAVNRVF 500 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,687,987,628 Number of extensions: 104593431 Number of successful extensions: 230137 Number of sequences better than 1.0e-05: 2756 Number of HSP's gapped: 224509 Number of HSP's successfully gapped: 2765 Length of query: 513 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 373 Effective length of database: 1,741,754,103 Effective search space: 649674280419 Effective search space used: 649674280419 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)